• Journal of Korean Society of Steel Construction
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• v.20 no.4
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• pp.539-548
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• 2008

This study aims to determine the applicability of the previously published T-type modular profile beam in the manner of producing specimens designed specially for the said purpose, determining their bending and shear behaviors depending on the presence of shear reinforcement, and analyzing the results in comparison with the theoretical equation of plastic deformation. The modular profile beam contributes to bending and shear resistance with the addition of the profile to the form function, and enhances the molding performance through the modular concept. The experimental results showed that the TS series specimens with shear reinforcement have bending behaviors superior to those of the T series specimens without shear reinforcement, which suggests that the used shear reinforcement appropriately bears the shear force. However, it was considered that all the specimens except for the T1-1 specimen failed to have adequate bending performance because of the intermodular slipping caused by the shear failure of the bolts. It is expected that further studies on the T-type modular profile beam, in which shear connectors will be considered as a variable,be performed to develop optimal intermodular connection methods.

• Journal of the Korean Society for Precision Engineering
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• v.1 no.3
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• pp.85-94
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• 1984

The WILDHABER-NOVIKOV gear, one of the circular arc gears, has the large contact area between the convex and concave profiled mating teeth, moves from one end of the tooth to the other axially making a face contact. Hence it provides a large load capacity than the Involute gear and still satisfying the law of gearing. In order to analyze the gear stress, a photoelastic investigation was carried out. Photo elastic model of the WILDHABER-NOVIKOV gears were made of Araldite CT200 in this investigation. For both the many teeth gear and the few teeth gear segments, External gears of all addendum type WILDHABER-NOVIKOV gear and the involute gear were tested. Included were the models with various profile raddi at the same pressure angle 20 .deg. and module 13.5. The flank stresses and fillet stresses of these gears were observed in each case and compared with those of gears. From this investigation, the following results were obtained. A. In the case of having many teeth gear: As the profile radius is increased, the fillet stresses of the WILDHABER-NOVIKOV gear become the same or less than that of the INVOLUTE gea, and the flank stress becomes smaller than that of the INVOLUTE gear. Therefore the better design condition is satisfied with a large profile radius. B. IN the case of having a few teeth gear: As the profile radius is increased the flank stress of WILDHABER-NOVIKOV gear becomes smaller than that of the INVOLUTE gear, but the fillet stresses become larger than that of the INVOLUTE gear. Therefore the larger design condition is satisfied with small profile radius.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.21 no.5
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• pp.135-140
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• 2021

In this paper, we propose an on-line mechanism that simultaneously detects cracks and profiling pavement blocks to detect the displacement of ground surface adjacent to the excavation in the urban area. The proposed method utilizes a 2D laser to profile the information about pavement blocks including the depth and distance among them. In particular, it is designed to enable the detection of cracks and portholes at runtime. For the experiment, real data was collected through Gocator, and trainng was carried out using Faster R-CNN. The performance evaluation shows that our detection precision and recall are more than 90% and the pavement blocks are profiled at the same time. Our proposed mechanism can be used for monitoring management to quantitatively detect the level of excavation risk before a large-scale ground collapse occurs.

• Genomics & Informatics
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• v.18 no.4
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• pp.42.1-42.9
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• 2020

Chromatin immunoprecipitation coupled with high-throughput DNA sequencing (ChIP-Seq) is a powerful technology to profile the location of proteins of interest on a whole-genome scale. To identify the enrichment location of proteins, many programs and algorithms have been proposed. However, none of the commonly used peak calling programs could accurately explain the binding features of target proteins detected by ChIP-Seq. Here, publicly available data on 12 histone modifications, including H3K4ac/me1/me2/me3, H3K9ac/me3, H3K27ac/me3, H3K36me3, H3K56ac, and H3K79me1/me2, generated from a human embryonic stem cell line (H1), were profiled with five peak callers (CisGenome, MACS1, MACS2, PeakSeq, and SISSRs). The performance of the peak calling programs was compared in terms of reproducibility between replicates, examination of enriched regions to variable sequencing depths, the specificity-to-noise signal, and sensitivity of peak prediction. There were no major differences among peak callers when analyzing point source histone modifications. The peak calling results from histone modifications with low fidelity, such as H3K4ac, H3K56ac, and H3K79me1/me2, showed low performance in all parameters, which indicates that their peak positions might not be located accurately. Our comparative results could provide a helpful guide to choose a suitable peak calling program for specific histone modifications.

• Genomics & Informatics
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• v.19 no.1
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• pp.8.1-8.12
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• 2021

Cytorace-3 is a laboratory evolved hybrid lineage of Drosophila nasuta nasuta males and Drosophila nasuta albomicans females currently passing ~850 generations. To assess interracial hybridization effects on gene expression in Cytorace-3 we profiled the transcriptomes of mature ovaries and testes by employing Illumina sequencing technology and de novo transcriptome assembling strategies. We found 26% of the ovarian, and 14% of testis genes to be differentially expressed in Cytorace-3 relative to the expressed genes in the parental gonadal transcriptomes. About 5% of genes exhibited additive gene expression pattern in the ovary and 3% in the testis, while the remaining genes were misexpressed in Cytorace-3. Nearly 772 of these misexpressed genes in the ovary and 413 in the testis were either over-or under-dominant. Genes following D. n. nasuta dominance was twice (270 genes) than D. n. albomicans dominance (133 genes) in the ovary. In contrast, only 105 genes showed D. n. nasuta dominance and 207 showed D. n. albomicans dominance in testis transcriptome. Of the six expression inheritance patterns, conserved inheritance pattern was predominant for both ovary (73%) and testis (85%) in Cytorace-3. This study is the first to provide an overview of the expression divergence and inheritance patterns of the transcriptomes in an independently evolving distinct hybrid lineage of Drosophila. This recorded expression divergence in Cytorace-3 surpasses that between parental lineages illustrating the strong impact of hybridization driving rapid gene expression changes.

• Journal of Ginseng Research
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• v.43 no.4
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• pp.572-579
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• 2019

Background: Panax ginseng has been used in traditional medicine to strengthen the body and mental well-being of humans for thousands of years. Many elite ginseng cultivars have been developed, and ginseng cultivation has become well established during the last century. However, heat stress poses an important threat to the growth and sustainable production of ginseng. Efforts have been made to study the effects of high temperature on ginseng physiology, but knowledge of the molecular responses to heat stress is still limited. Methods: We sequenced the transcriptomes (RNA-Seq) of two ginseng cultivars, Chunpoong (CP) and Yunpoong (YP), which are sensitive and resistant to heat stress, respectively, after 1- and 3-week heat treatments. Differential gene expression and gene ontology enrichment along with profiled chlorophyll contents were performed. Results: CP is more sensitive to heat stress than YP and exhibited a lower chlorophyll content than YP. Moreover, heat stress reduced the chlorophyll content more rapidly in CP than in YP. A total of 329 heat-responsive genes were identified. Intriguingly, genes encoding chlorophyll a/b-binding proteins, WRKY transcription factors, and fatty acid desaturase were predominantly responsive during heat stress and appeared to regulate photosynthesis. In addition, a genome-wide scan of photosynthetic and sugar metabolic genes revealed reduced transcription levels for ribulose 1,5-bisphosphate carboxylase/oxygenase under heat stress, especially in CP, possibly attributable to elevated levels of soluble sugars. Conclusion: Our comprehensive genomic analysis reveals candidate loci/gene targets for breeding and functional studies related to developing high temperature-tolerant ginseng varieties.

• Journal of Digital Convergence
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• v.17 no.4
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• pp.355-364
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• 2019

The purpose of this study is to present the implications of analyzing users' market segmentation and characteristics based on the tourism SNS lovemarks experience. The results are as follows. First as a result of conducting factor analysis of tourism SNS lovemarks experience, five factors explained namely, mystery, sensuality, intimacy, trust, reputation. Second, as a result of conducting cluster analysis, four cluster groups were identified (reputation/sensuality centered experience group, high lovemarks experience group, intimacy centered experience group, low lovemarks experience group) and each characteristics were profiled. Third, as a result of Chi-square analysis and correspondence analysis, there was a difference in the characteristics of tourism SNS usage by cluster. Fourth, higher tourism SNS lovemarks experience segmented groups were more likely to recognize the degree of experiential value, satisfaction, behavioral intention.

• Journal of Animal Reproduction and Biotechnology
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• v.36 no.1
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• pp.35-44
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• 2021

A pregnancy diagnosis is an important standard for control of livestock's reproduction in paricular dairy cattle. High reproductive performance in dairy animals is a essential condition to realize of high life-time production. Pregnancy diagnosis is crucial to shortening the calving interval by enabling the farmer to identify open animals so as to treat or re-breed them at the earliest opportunity. MicroRNAs are short RNA molecules which are critically involved in regulating gene expression during both health and disease. This study is sought to establish the feasible of circulating miRNAs as biomarkers of early pregnancy in cattle. We applied Illumina small-RNA sequencing to profile miRNAs in plasma samples collected from 12 non-pregnant cows ("open" cows: samples were collected before insemination (non-pregnant state) and after pregnancy check at the indicated time points) on weeks 0, 4, 8, 12 and 16. Using small RNA sequencing we identified a total of 115 miRNAs that were differentially expressed weeks 16 relative to non-pregnancy ("open" cows). Weeks 8, 12 and 16 of pregnancy commonly showed a distinct increase in circulating levels of miR-221 and miR-320a. Through genome-wide analyses we have successfully profiled plasma miRNA populations associated with pregnancy in cattle. Their application in the field of reproductive biology has opened up opportunities for research communities to look for pregnancy biomarker molecules in dairy cattle.

• Journal of Animal Science and Technology
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• v.63 no.6
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• pp.1411-1422
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• 2021

Lactobacillus acidophilus is a gram-positive, microaerophilic, and acidophilic bacterial species. L. acidophilus strains in the gastrointestinal tracts of humans and other animals have been profiled, but strains found in the canine gut have not been studied yet. Our study helps in understanding the genetic features of the L. acidophilus C5 strain found in the canine gut, determining its adaptive features evolved to survive in the canine gut environment, and in elucidating its probiotic functions. To examine the canine L. acidophilus C5 genome, we isolated the C5 strain from a Korean dog and sequenced it using PacBio SMRT sequencing technology. A comparative genomic approach was used to assess genetic relationships between C5 and six other strains and study the distinguishing features related to different hosts. We found that most genes in the C5 strain were related to carbohydrate transport and metabolism. The pan-genome of seven L. acidophilus strains contained 2,254 gene families, and the core genome contained 1,726 gene families. The phylogenetic tree of the core genes in the canine L. acidophilus C5 strain was very close to that of two strains (DSM20079 and NCFM) from humans. We identified 30 evolutionarily accelerated genes in the L. acidophilus C5 strain in the ratio of non-synonymous to synonymous substitutions (dN/dS) analysis. Five of these thirty genes were associated with carbohydrate transport and metabolism. This study provides insights into genetic features and adaptations of the L. acidophilus C5 strain to survive the canine intestinal environment. It also suggests that the evolution of the L. acidophilus genome is closely related to the host's evolutionary adaptation process.

• Molecules and Cells
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• v.45 no.8
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• pp.588-602
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• 2022

Various RNA-binding proteins (RBPs) are key components in RNA metabolism and contribute to several neurodevelopmental disorders. To date, only a few of such RBPs have been characterized for their roles in neocortex development. Here, we show that the RBP, Rbms1, is required for radial migration, polarization and differentiation of neuronal progenitors to neurons in the neocortex development. Rbms1 expression is highest in the early development in the developing cortex, with its expression gradually diminishing from embryonic day 13.5 (E13.5) to postnatal day 0 (P0). From in utero electroporation (IUE) experiments when Rbms1 levels are knocked down in neuronal progenitors, their transition from multipolar to bipolar state is delayed and this is accompanied by a delay in radial migration of these cells. Reduced Rbms1 levels in vivo also reduces differentiation as evidenced by a decrease in levels of several differentiation markers, meanwhile having no significant effects on proliferation and cell cycle rates of these cells. As an RNA binding protein, we profiled the RNA binders of Rbms1 by a cross-linked-RIP sequencing assay, followed by quantitative real-time polymerase chain reaction verification and showed that Rbms1 binds and stabilizes the mRNA for Efr3a, a signaling adapter protein. We also demonstrate that ectopic Efr3a can recover the cells from the migration defects due to loss of Rbms1, both in vivo and in vitro migration assays with cultured cells. These imply that one of the functions of Rbms1 involves the stabilization of Efr3a RNA message, required for migration and maturation of neuronal progenitors in radial migration in the developing neocortex.