• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.30 no.3
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• pp.186-192
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• 2004

Purpose: Human tooth proteins are highly heterogeneous, comprising diverse proteins derived from a number of genes. The attempts to identify protein for activity of tooth matrix proteins have been defied by several factors. First, the amount of proteins within teeth is very small relative to many extracellular matrix proteins of other tissues. Second, the bioassay system is tedious and needed for long time. Therefore we tried to find easy techniques, which increase the product rate, and an assay of small proteins, with which amino acid sequence is possible without additional procedures. Materials and Methods: Total protein were extracted from 300 g enamel removed teeth and 600 g teeth with 4 mol/L guanidine HCl and purified by gel chromatography. Aliquot of proteins was implanted into muscle pouches in Sprague-Dawley rats for bioassay. By SDS-PAGE and membrane blotting, molecular weight of each protein was estimated and a partial amino acid sequence was obtained. Each fraction blotted on the membrane was cut out and inserted in rat ectopic model. Results: In dissociative method, total tooth proteins were obtained 1mg/ml from enamel removed teeth and 3.5 mg/ml from teeth. In SDS-PAGE, four clear bands at the sites corresponding to 66, 40, 20 and 18 kD. Especially The 66 kD band was clearly exhibited. Amino acid sequencing from tooth could be possible using PVDF membrane blotting technique. In amino acid sequencing, 66 kD protein was identified as albumin. Conclusion: Compared with conventional method for extraction of teeth protein and bioassay of proteins, the methods in this study were easy, time-saving and more productive technique. The matured tooth proteins omitting additional procedure of mechanical removal of enamel were simply analyzed using blotted PVDF membrane. This method seems to make a contribution as a technique for bioassay and amino acid sequencing of protein.

• Mycobiology
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• v.46 no.3
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• pp.269-277
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• 2018

The production of water-soluble pigments by fungal strains indigenous to South Korea was investigated to find those that are highly productive in submerged culture. Among 113 candidates, 34 strains that colored the inoculated potato dextrose agar medium were selected. They were cultured in potato dextrose broth and extracted with ethanol. The productivity, functionality (radical-scavenging activities), and color information (CIELAB values) of the pigment extracts were measured. Five species produced intense yellowish pigments, and two produced intense reddish pigments that ranked the highest in terms of absorbance units produced per day. The pigment extracts of Penicillium miczynskii, Sanghuangporus baumii, Trichoderma sp. 1, and Trichoderma afroharzianum exhibited high radical-scavenging activity. However, the S. baumii extract showed moderate toxicity in the acute toxicity test, which limits the industrial application of this pigment. In conclusion, P. miczynskii KUC1721, Trichoderma sp. 1 KUC1716, and T. afroharzianum KUC21213 were the best fungal candidates to be industrial producers of safe, functional water-soluble pigments.

• Journal of Digital Convergence
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• v.11 no.11
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• pp.499-508
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• 2013

This study was done to examine the affecting factors of successful aging to provide basic information for developing intervention program for the rural elderly. Methods: A cross-sectional descriptive design was used. Data was collected through questionnaire from 208 people with age over 65 residing in an rural community. They were recruited by convenience sampling. Collected data were analyzed by independent t-test, one-way ANOVA, Pearson's correlation coefficients, and multiple regression through SPSS/WIN 21.0 program. Results: Explanatory factors affecting the rural elderly's successful aging were social support, depression, cognitive function, productive activity and Activities of daily living, respectively, and these five factors accounted for 42.1% of the variance in successful aging. Conclusion: The rural elderly's successful aging requires establishment of social support system such as health medical service supply and social activities by being connected with social communities, and for this, this study suggests that there be a necessity of developing customized nursing intervention program.

• Molecules and Cells
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• v.40 no.1
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• pp.24-36
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• 2017

The stability of peptide-MHC complex (pMHC) is an important factor to shape the fate of peptide-specific T cell immune response, but how it influences on T cell activation process is poorly understood. To better understand that, we investigated various T cell activation events driven by $L^d$ MHCI loaded with graded concentrations of P2Ca and QL9 peptides, respectively, with 2C TCR Tg T cells; the binding strength of P2Ca for $L^d$ is measurably weaker than that of QL9, but either peptides in the context of $L^d$ interact with 2C TCR with a similar strength. When their concentrations required for early T cell activation events, which occur within several minutes to an hour, were concerned, $EC_{50}s$ of QL9 were about 100 folds lower than those of P2Ca, which was expected from their association constants for $L^d$. When $EC_{50}s$ for late activation events, which takes over several hours to occur, were concerned, the differences grew even larger (> 300 folds), suggesting that, due to weak binding, $L^d/P2Ca$ dissociate from each other more easily to lose its antigenicity in a short time. Accordingly, fixation of $L^d/P2Ca$ with paraformaldehyde resulted in a significant improvement in its immunogenicity. These results imply that binding strength of a peptide for a MHC is a critical factor to determine the duration of pMHC-mediated T cell activation and thus the attainment of productive T cell activation. It is also suggested that paraformaldehyde fixation should be an effective tool to ameliorate the immunogenicity of pMHC with a poor stability.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.4
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• pp.1833-1843
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• 2011

Recently, Our society socioeconomically has faced the sudden changes. The retirement of the baby-boom generation is expected to become mainstream amid the falling birth rate and the aging society at the fastest rate in the world. As such a baby-boom generation is a core pillar of our economic development in the 1970s and 1980s, the rehiring difficulties after retirement, the insignificant old age preparation and their welfare and medical problem has become national issue. The government has implemented the policy for promoting the senior entrepreneur to participate productive economic activity by actively utilizing the senior's strength from this year. However, in spite of the importance of such a senior entrepreneur, the research to study the senior's desire and the concrete characteristics of the senior entrepreneur is nonexistence. Therefore, this paper empirically analysed the senior's concrete nature, entrepreneurial intention and the impact on the senior entrepreneurship, which is the core of the success entrepreneur and provided stimulating measure.

• Animal Bioscience
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• v.34 no.3_spc
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• pp.443-448
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• 2021

Objective: Astaxanthin is a natural super antioxidant. The present study was carried out to investigate the effect of astaxanthin rich Phaffia rhodozyma (PR) supplementation in diets on laying production performance, egg quality, antioxidant defenses and immune defenses in laying hens. Methods: A total of five hundred and twelve 60-week-old Lohmann Brown laying hens (2,243±12 g) were randomly assigned to four groups, each including 4 replicates with 32 birds per replicate. Astaxanthin rich PR was added to corn-soybean meal diets to produce experimental diets containing 0 (Control), 800 mg/kg, 1,200 mg/kg, and 1,600 mg/kg PR, respectively. The astaxanthin content in the diet was 0.96 mg/kg, 1.44 mg/kg and 1.92 mg/kg respectively. Results: Results showed that dietary PR supplementation tended to increase daily feed intake (p = 0.0512). There was no effect of astaxanthin rich PR on Haugh units, albumen height, egg shape index, eggshell strength, and eggshell thickness at weeks 6 (p>0.05). However, egg yolk color was significantly improved (p<0.05). In addition, astaxanthin rich PR supplementation significantly increased serum glutathione peroxidase and superoxide dismutase activity (p<0.05), increased serum immunoglobulin G content (p<0.05), and reduced malondialdehyde content (p<0.05) in laying hens. Conclusion: In conclusion, astaxanthin rich PR can improve the color of egg yolk, enhance the antioxidant defenses, and regulate the immune function.

• Journal of Environmental Science International
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• v.32 no.12
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• pp.955-964
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• 2023

This study was conducted to obtain basic data on efficient and eco-friendly crop cultivation for urban residents who enjoy urban agriculture as a hobby or leisure activity. We planted strawberry(Fragaria x ananassa)(S) and kidney bean (Phaseolus vulgaris var. humilis)(K) in different ratios to analyze the differences in soil environment, growth, physiology, and productivity, and to investigate the effects. Strawberry growth was optimal with S1K2 ratio, whereas the S1K1 ratio treatment showed the highest levels of physiology and productivity. In terms of growth and physiology, kidney beans tended to perform best in S1K2 treatment. The average number of productive pods was two, with a highest average value of 2.3 being recorded in S2K1 treatment planted with a high percentage of strawberries. In terms of growth, physiology, and prodctivity our findings indicate that it would be desirable to plant starwberries and kidney bean in a 1:1 ratio. However, considering the environmental characteristics of walls and rooftops, it is necessary to effectively manage crops suitable for these conditions. Furthermore, additional studies should be conducted to analyze the quality of fruits and seeds producted, both qualitatively and quantitatively in the future.

• The Journal of the Korea institute of electronic communication sciences
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• v.8 no.6
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• pp.933-940
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• 2013

Our country is defenseless against the aging population in society that is suddenly and rapidly increasing. This is expressed as the aging shock and aged society earthquake, warning us of the crisis in the future society. Senior citizens of Korea are a group that does not receive proper education due to various social factors, and it is a group that requires lifelong education more than any other group, and therefore, senior citizens have become a priority target for lifelong education. Thus, the need for lifelong education for seniors can be said to be in establishing a new senior citizen image so that they may live a fuller retirement by improving their abilities to appropriately adapt to the rapidly changing modern society due to the digital times. Furthermore, with the enhanced cultural level and personal desire for a fuller life in the modern society, the necessity for leisure activities has been emphasized. Instead of simply relieving stress through leisure activities, it aims at being provided with educational opportunities through leisure activities. Therefore, senior leisure activity education should be an important method for establishing the senior citizen identity of a self-oriented senior, productive senior, volunteering senior, informed senior, and a well-rounded senior, which are all the image of senior citizens in the 21st century. In addition, a specialized leisure activity program that systematically provides education for leisure activities should be performed from the perspective of lifelong education. Hence, the significance of senior citizen leisure activities as lifelong education is in improving health, enhancing sociability and realizing one's self, while improving the quality of life for senior citizens. It will be greatly meaningful for establishing the new senior citizen image in which they independently live their own lives, while not being dependent on others, as required in the 21st century.

• Journal of Service Research and Studies
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• v.7 no.2
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• pp.1-16
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• 2017

The service that has been recognized as both a non-productive activity and auxiliary activity of manufacturing have become the driving force of the customers' demand with the 'service' itself. The service base is expanding and evolving rapidly. It is important to look at changes in service concepts to understand service systems. Because the service system itself has a cyclic nature based on the concept of service, it can help in the study and the "how" of service by looking at changing the system according to the evolution of the service concept. The ability to organize and utilize relationships is considered to be an important factor for managers in the service economy era. However, the attention of corporate is focused on their internal capabilities and they are familiar with external resources (knowledge and competence of customers). In this case study for each type of service, we analyzed the activities of interacting service providers-consumers in service relationship, and constructed a new service system model emphasizing intangible value and long-term outcome. This study is worth re-examining the role of customers in today's service economy era and actively utilizing a new service model for business performance.

• Review of Culture and Economy
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• v.17 no.2
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• pp.103-135
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• 2014

This research dwells on the new paradigm of policy making of some innovative nations in Korean content industry. Especially this paper did evaluation of governmental policy in recent 5 years (2008~2012) in the field of Korean content industry. According to this research, the program of policy in Korean content industry had been obsessed to passive enrichment focusing on some part of content industry. The whole process of policy, scheme of policy and working flow of policy were very constrained in order to encourage more measurable area like CT(culture technology). Thus, we need new strong policy in this new government launched in 2013. The apparent keyword should be 'management'. New activity of policy need to focus on encourage Korean content industry in the real site of field, not in bureaucratic office in remote site. This change reflecting real filed management system would be productive innovation for policy making and activity in Korea.