• KSBB Journal
• /
• 제28권3호
• /
• pp.213-215
• /
• 2013

A high-throughput screening strategy was developed to simplify the selection process for improved mannitol producing strain after chemical mutagenesis. Ethylmethyl sulfonate (EMS) was used as a chemical mutagen to alter the fructokinase-I gene which is an essential enzyme to metabolize fructose for growth. Leuconostoc mesenteroides treated with EMS were plated on the modified MRS solid medium containing fructose as a sole carbon source. Strains showing inhibited growth were primarily selected to evaluate the mannitol producing ability. By applying this strategy, L. mesenteroides ATCC 8293 M1, L. mesenteroides ATCC 9135 M3 and L. mesenteroides D1 M3 showed improvement in mannitol production.

• International Journal of Quality Innovation
• /
• 제2권1호
• /
• pp.106-116
• /
• 2001

The purpose of business strategy is to achieve competitive advantages which includes higher efficiency, better quality, more innovation and faster customer response. In other words, The business strategy is to build unique capability of lower cost and/or differentiation. In production aspect, unique capability means better production power with better performance at 3M(Man, Machine, Material) of input and PQCDSM (Product, Quality, Cost, Delivery, Safety, Moral) from output. The Total Productive management (TPM), a series of improvement activities focused on reduction of equipment loss, is a tool to establish business competitive advantages. In this paper, several domestic companies who won the Japan TPM Award have been studied. It is found that there is a strong cause-effect relationship between TPM and competitive advantages because. 1. TPM can change employees mindset effectively. 2. TPM can upgrade employees capabilities. 3. TPM can lead to excellent productivity.

• 대한안전경영과학회지
• /
• 제7권3호
• /
• pp.121-135
• /
• 2005

The TOC and Six Sigma are the most attention-getting concepts for managing manufacturing companies. Using the ideas and methods of the TOC, companies can achieve a large reduction of work-in-process and finished good inventories, significant improvement in scheduling performance, and substantial earnings increase. The six sigma approach derives the overall process of selecting the right project based on their potential to improve performance metrics and selecting and training the right people to get the business results. These two concepts have different backgrounds and different viewpoints for production systems. so, if the two concepts integrate each other, the synergy effects to innovate production systems can be expected. The purpose of this paper suggest that integration strategy between Six Sigma and TOC for profit maximization.

• 대한안전경영과학회:학술대회논문집
• /
• 대한안전경영과학회 2011년도 추계학술대회
• /
• pp.557-561
• /
• 2011

The study proposes three types of production lead time according to the production or demand pattern. First of all, it discusses the difference of three lead times. While pitch time and cycle time are used in push system with process stock and mass conveyor production, the tact time is used in pull system like as JIT based lean production system.

• 한국CDE학회논문집
• /
• 제15권2호
• /
• pp.94-105
• /
• 2010

Todays, there is fast transition about the new manufacturing IT methodologies from the conceptual phase into the practical application phase for the strengthening of enterprise competitiveness in manufacturing industry. One of those new methodologies is PLM (Product Life-cycle Management). PLM methodology consists of 3D CAD for the product design, PDM (Product Data Management) for the data management based on the collaboration platform and lastly DM (Digital Manufacturing). DM has evoluted from the stand-alone computer simulation of early 1980s, and now it covers the overall production development and production. Unfortunately, there exist serious critical problems about the actual application of DM for the real work. This is owing to the transition of the point of view from stand-alone type application (such as flow simulation or robot simulation) to that of business process about product development and production management. In this paper, we propose an application framework for the successful project with the digital manufacturing methodology with the concept of Information Strategy Planning, which enables the systematic diagnosis and the quantitative evaluation. Also, we introduce the actual practice of the proposing framework with the ISP project for 'Analysis & Simulation Technique of manufacturing process project' that is being conducted by Chungnam Techno Park.

• Spatial Information Research
• /
• 제20권6호
• /
• pp.69-76
• /
• 2012

본 연구에서는 산업연관분석을 통해 공간정보산업의 특징을 도출하였다. 이를 위하여 공간정보산업을 재분류하고 이에 맞게 산업연관표를 수정하여 공간정보산업을 분석하였으며 생산유발계수, 영향력계수, 감응력계수를 도출하였다. 그 결과 공간정보산업은 생산유발효과는 작으나 전방연쇄효과가 매우 큰 산업으로 확인되었다. 이를 기반으로 공간정보산업 육성을 위한 정책의 방향성을 '산업생태계 조성, 고도화와 활용도 향상 병행, 장기적 관점 수립'으로 제시하였다.

• 농업생명과학연구
• /
• 제44권2호
• /
• pp.85-96
• /
• 2010

본 연구에서는 우리나라의 대표적 수입 곡물인 콩의 주요 교역국들의 생산, 소비, 무역 및 재고 동향과 각국의 생산변동에 따른 조정유형 분석을 수행한 후, 이를 바탕으로 각국의 국제시장 전이효과를 계측하였다. 분석결과 최근 콩의 국제시장 전이효과가 주요 생산국인 브라질, 파라과이, 캐나다 등을 중심으로 증대되고 있으며, 대부분의 국가가 재고보다는 소비와 무역을 통해 생산변동을 조절하고 있는 것으로 나타나 국제 콩시장의 불안정성 증가가 예상된다. 따라서 대규모 콩 수입국인 우리나라는 일정량의 국내생산기반 유지, 적절한 재고정책의 활용, 선물시장의 활용, 수입선 다변화 등과 같은 해외조달능력의 향상을 통한 안정적인 국내 콩 수급 방안 마련이 시급한 것으로 나타났다.

• KSBB Journal
• /
• 제25권2호
• /
• pp.187-192
• /
• 2010

S. cerevisiae ATCC 24858을 이용한 ethanol 생산에서, aeration 효과를 ethanol 수율, specific ethanol production rate, ethanol 생산성 측면에서 분석하여, 반복 유가식 공정전략을 설계하였다. Ethanol 수율과 ethanol 생산성은 공기를 0.33 vvm 넣었을 때, 공기를 넣지 않고 배양한 것에 비하여 더 큰 값을 보였고, 24시간 마다 배지를 교체한 배양이 36시간 마다 배지를 교체한 배양 보다 더 큰 값을 보였다. 총 ethanol 생산량 값이 가장 큰 경우는 0.33 vvm의 공기를 넣고, 배지를 24시간마다 완전히 갈아주었을 때이고, 이때 가장 많은 703.8 g의 ethanol이 생산되었다.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2002년도 생물공학의 동향 (X)
• /
• pp.293-296
• /
• 2002

Hyperosmotic pressure increased specific antibody productivity ($q_{Ab}$) of recombinant CHO cells (SH2-0.32) while it depressed cell growth. Thus, the use of hyperosmolar medium did not increase the maximum antibody concentration substantially. To overcome this drawback, the feasibility of biphasic culture strategy was investigated. In the biphasic culture, cells were first cultivated in the standard medium with physiological osmolality(294 mOsm/kg) for cell growth. When cells reached the late exponential phase of growth, the spent standard medium was replaced with the fresh hyperosmolar medium (522 mOsm/kg) for antibody production. The ($q_{Ab}$) in growth phase with the standard medium was 2.1 ${\mu}g/10^6cell/day$ while the ($q_{Ab}$) in antibody production phase with the hyperosmolar medium (522 mOsm/kg) was 11.1 ${\mu}g/10^6cell/day$. Northern blot analysis showed a positive relationship between the relative contenet of Ig mRNA and ($q_{Ab}$), indicating that transcriptional regulation was involved in the response of rCHO cells to hyperosmotic pressure. Due to the enhanced ($q_{Ab}$) and increased cell concentration in biphasic culture, the maximum antibody concentration obtained in biphasic culture with 522 mOsm/kg medium exchange was 161% higher than that obtained in batch culture with the standard medium. Taken together, simple biphasic culture strategy based on hyperosmotic culture for improved foreign protein production from rCHO cells is effective in improving antibody production of rCHO cells.

• Journal of Microbiology and Biotechnology
• /
• 제26권3호
• /
• pp.498-502
• /
• 2016

3'-Hydroxygenistein can be obtained from the biotransformation of genistein by the engineered Pichia pastoris X-33 strain, which harbors a fusion gene composed of CYP57B3 from Aspergillus oryzae and a cytochrome P450 oxidoreductase gene (sCPR) from Saccharomyces cerevisiae. P. pastoris X-33 mutants with higher 3'-hydroxygenistein production were selected using a periodic hydrogen peroxide-shocking strategy. One mutant (P2-D14-5) produced 23.0 mg/l of 3'-hydroxygenistein, representing 1.87-fold more than that produced by the recombinant X-33. When using a 5 L fermenter, the P2-D14-5 mutant produced 20.3 mg/l of 3'-hydroxygenistein, indicating a high potential for industrial-scale 3'-hydroxygenistein production.