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http://dx.doi.org/10.7841/ksbbj.2013.28.3.213

Chemical Mutation of Leuconostoc mesenteroides for Improved Mannitol Production: Development of a High-throughput Screening Strategy  

Lee, Hyeong Rho (Department of Food Science and Technology, Chungbuk National University)
Ahn, Ji Eun (Department of Food Science and Technology, Chungbuk National University)
Han, Nam Soo (Department of Food Science and Technology, Chungbuk National University)
Publication Information
KSBB Journal / v.28, no.3, 2013 , pp. 213-215 More about this Journal
Abstract
A high-throughput screening strategy was developed to simplify the selection process for improved mannitol producing strain after chemical mutagenesis. Ethylmethyl sulfonate (EMS) was used as a chemical mutagen to alter the fructokinase-I gene which is an essential enzyme to metabolize fructose for growth. Leuconostoc mesenteroides treated with EMS were plated on the modified MRS solid medium containing fructose as a sole carbon source. Strains showing inhibited growth were primarily selected to evaluate the mannitol producing ability. By applying this strategy, L. mesenteroides ATCC 8293 M1, L. mesenteroides ATCC 9135 M3 and L. mesenteroides D1 M3 showed improvement in mannitol production.
Keywords
Leuconostoc mesenteroides; Mannitol; Ethylmethyl sulfonate (EMS); High-throughput screening;
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1 Khan, A., A. Bhide, and R. Gadre (2009) Mannitol production from glycerol by resting cells of Candida magnoliae. Bioresour. Technol. 100: 4911-4913.   DOI   ScienceOn
2 Von Weymarn, N., M. Hujanen, and M. Leisola (2002) Production of D-mannitol by heterofermentative lactic acid bacteria. Process Biochem. 37: 1207-1213.   DOI   ScienceOn
3 Soetaert, W., P. T. Vanhooren, and E. J. Vandamme (1999) Production of mannitol by fermentation. Methods Biotechnol. 10: 261-275.   DOI
4 Song, K. H., J. K. Song, S. G. Hong, H. Baek, S. Y. Kim, and H. H. Hyun (2002) Production of mannitol by a novel strain of Candida magnoliae. Biotechnol. Lett. 24: 9-12.   DOI   ScienceOn
5 Wisselink, H., R. A. Weusthuis, G. Eggink, J. Hugenholtz, and G. J. Grobben (2002) Mannitol production by lactic acid bacteria: A review. Int. Dairy J. 12: 151-161.   DOI   ScienceOn
6 Saha, B. C. and F. M. Racine (2010) Effects of pH and corn steep liquor variability on mannitol production by Lactobacillus intermedius NRRL B-3693. Appl. Microbiol. Biotechnol. 87: 553-560.   DOI
7 Helanto, M., J. Aarnikunnas, N. von Weymarn, U. Airaksinen, A. Palva, and M. Leisola (2005) Improved mannitol production by a random mutant of Leuconostoc pseudomesenteroides. J. Biotechnol. 116: 283-294.   DOI   ScienceOn
8 Ghoreishi, S. and R.G. Shahrestani (2009) Innovative strategies for engineering mannitol production. Trends Food Sci. Technol. 20: 263-270.   DOI   ScienceOn