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http://dx.doi.org/10.4014/jmb.1509.09013

Improving 3'-Hydroxygenistein Production in Recombinant Pichia pastoris Using Periodic Hydrogen Peroxide-Shocking Strategy  

Wang, Tzi-Yuan (Biodiversity Research Center, Academia Sinica)
Tsai, Yi-Hsuan (Department of Biological Sciences and Technology, National University of Tainan)
Yu, I-Zen (Department of Biological Sciences and Technology, National University of Tainan)
Chang, Te-Sheng (Department of Biological Sciences and Technology, National University of Tainan)
Publication Information
Journal of Microbiology and Biotechnology / v.26, no.3, 2016 , pp. 498-502 More about this Journal
Abstract
3'-Hydroxygenistein can be obtained from the biotransformation of genistein by the engineered Pichia pastoris X-33 strain, which harbors a fusion gene composed of CYP57B3 from Aspergillus oryzae and a cytochrome P450 oxidoreductase gene (sCPR) from Saccharomyces cerevisiae. P. pastoris X-33 mutants with higher 3'-hydroxygenistein production were selected using a periodic hydrogen peroxide-shocking strategy. One mutant (P2-D14-5) produced 23.0 mg/l of 3'-hydroxygenistein, representing 1.87-fold more than that produced by the recombinant X-33. When using a 5 L fermenter, the P2-D14-5 mutant produced 20.3 mg/l of 3'-hydroxygenistein, indicating a high potential for industrial-scale 3'-hydroxygenistein production.
Keywords
3'-Hydroxygenistein; orobol; CYP57B3; Pichia pastoris;
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