• 한국버섯학회지
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• 제13권1호
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• pp.79-83
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• 2015

본 연구는 큰느타리버섯의 베타글루칸 고함유 형질에 관련된 SCAR marker를 개발하기 위해 수행되었다. operon사의 OPA(20개), OPB(20개), OPL(20개), OPP(20개), OPR(20개), OPS(20개) 등 총 120개 primer를 random primer(10 mer)로 사용하여 대립 계통 9종과 베타글루칸 고함유 계통 9 종을 대상으로 RAPD를 이용한 bulked segregant analysis를 실시하여 OP-R03 primer로부터 대립 계통에는 나타나지 않고 베타글루칸 고함유 계통에만 나타나는 특이적인 RAPD 밴드를 얻었다. OP-R03 primer를 이용한 RAPD 결과, 약 91 bp 부근에서 베타글루칸 고함유 계통에 특이적인 DNA 밴드가 관찰되었으며 이 DNA 밴드의 염기서열 말단을 근거로 SCAR 마커로 사용할 specific primer인 OP-R03-1-F와 OP-R03-1-R를 디자인하였다. SCAR 마커 OP-R03-1-F/-1-R primer를 이용하여 PCR을 수행한 결과에서도 91 bp 부근에서 대립 계통과 구별되는 DNA 밴드가 베타글루칸 고함유 계통에서 확인되었으며 random primer인 OP-R03 primer를 이용하여 PCR을 수행했을 때보다 재현성이 높고 진한 DNA 밴드임을 확인할 수 있었다.

• 한국버섯학회지
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• 제12권3호
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• pp.226-231
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• 2014

본 연구는 큰느타리버섯의 고온적응성 형질에 관련된 SCAR marker를 개발하기 위해 수행되었다. operon 사의 OPA(20개), OPB(20개), OPL(20개), OPP(20개), OPR(20개), OPS(20개) 등 총 120개 primer를 random primer(10 mer)로 사용하여 대립 계통 7종과 고온성 계통 7 종을 대상으로 RAPD를 이용한 bulked segregant analysis를 실시하여 OP-A06 primer로부터 대립 계통에는 나타나지 않고 고온성 계통에만 나타나는 특이적인 RAPD 밴드를 얻었다. OP-A06 primer를 이용한 RAPD 결과, 약 385 bp 부근에서 고온성 계통에 특이적인 DNA 밴드가 관찰되었으며 이 DNA 밴드의 염기서열 말단을 근거로 SCAR 마커로 사용할 specific primer인 OP-A06-1-F와 OP-A06-1-R를 디자인하였다. SCAR 마커 OP-A06-1-F/-1-R primer를 이용하여 PCR을 수행한 결과에서도 385 bp 부근에서 대립 계통과 구별되는 DNA 밴드가 고온성 계통에서 확인되었으며 random primer인 OP-A06 primer를 이용하여 PCR을 수행했을 때보다 재현성이 높고 진한 DNA 밴드임을 확인할 수 있었다.

• Rapid Communication in Photoscience
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• 제3권4호
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• pp.76-78
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• 2014

Fluorescent nucleic acids were prepared utilizing the polymerase extension (PEX) reaction to incorporate fluorescent molecules. 2'-Deoxyuridine triphosphate (dUTP) derivatives possessing pyrene molecules as fluorophores were synthesized using the aqueous-phase Sonogashira coupling between 5-Iodo-dUTP and acetylene-linked pyrene molecules. The incorporation of the pyrene (Py)-labeled deoxyuridine triphosphates (PyU) into DNA by polymerase was evaluated by polyacrylamide gel electrophoresis, demonstrating that the PyU can work as a good substrate for the PEX reaction. The fluorescent properties of the functionalized DNA prepared by the PEX reaction were characterized by steady-state fluorescence measurements. The Py-conjugated DNA showed typical emission spectra of the pyrene, and the DNA with two pyrene molecules connected to each other by a diethylene glycol linker exhibited a broadened emission attributed to the electronic interaction between the Py molecules.

• 식물병연구
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• 제13권1호
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• pp.71-73
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• 2007

전라남도 호박 주산단지의 2000년 바이러스병 발생포장율은 노지재배 76.1%,억제재배 55%,반촉성재배는 발생이 없었으며, 포장별 이병주율은 반촉성재배 3.6%, 노지재배 100%이었다. 포장에서 채집된 시료에 대한 RT-PCR 검정결과 노지재배는 40점 시료 중 WMV 16점, ZYMV 10점, PRSV 2점이 각각 검출되었으며, WMV, ZYMV, PRSV의 2개 또는 3개 바이러스에 중복감염된 시료도 7점이었다. 억제재배는 21점 시료중 WMV 7점, ZYMV 6점, PRSV 6점, WMV와 PRSV의 2개 바이러스에 중복감염된 시료가 1점이었으며, CMV는 검출되지 않았다.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 1990년도 Proceedings of International Symposium on Korean Ginseng, 1990, Seoul, Korea
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• pp.65-67
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• 1990

The sequence of ginseng peptide gene was designed and synthesized by the solid phase plasmid pUC19. Escherichia coli JM101 cells were transformed with above hybrid plasmids. Ampicillin resistant transformants were screened and identified by in situ colony hybridization and Southern blot techinques. Finally the gene sequencing was done by the Sanger dideoxy method using primer extension.

• Journal of Ginseng Research
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• 제14권2호
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• pp.207-209
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• 1990

The sequence of ginseng peptide gene was designed and synthesized by the solid phase phosphoramidiate method. Synthetic segments were isolated, pllrified and joined to the plasmid pUC19. E.icherichiu coli JM101 cells were transformed with above hybrid plasmids. AmpiciIBin resistant transformants were screened and identified by in situ colony hybridization and Southern blot techniques. Finally the gene sequencing was done by the Sanger dideoxy method using primer extension.

• Journal of Forest and Environmental Science
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• 제31권1호
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• pp.68-71
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• 2015

Korean L. leiolepis of the genus Leontopodium could be discriminate from the foreign L. alpinum using random amplified polymorphic DNA (RAPD). Among the 12 URP markers used for the detection, the URP-5 marker and the URP-7 marker detected polymorphic DNA bands, ranging from 400-1000 bp in the size of amplified DNA fragments.

• Bulletin of the Korean Chemical Society
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• 제30권11호
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• pp.2769-2772
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• 2009

• Corrosion Science and Technology
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• 제20권4호
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• pp.169-174
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• 2021

In this study, performance tests, a field evaluation, and a life cycle cost (LCC) analysis for high performance coating systems were conducted to prepare a plan to reduce the cost of maintenance coating and contribute to the service life extension of steel bridges by applying high performance coatings to steel bridges that will be constructed in the future. From the deterioration models based on the field evaluation for chlorinated rubber and urethane topcoat systems, which have been applied often, the mean service lives were derived as 20.8 and 26.6 years, respectively. For the other coating systems that have not been applied in practice, the coordination factors were differentially applied with evaluation items. The most durable coating system was predicted to be thermal spray coating (TSC) primer/epoxy intermediate coat/fluoride resin topcoat, with a predicted value as long as 42.2 years. The LCC analysis indicates that partial application of high performance coating, such as TSC and fluoride resin, to specific parts vulnerable to corrosion and ultraviolet ray (UV) is more advantageous than the use of general coating systems.

• The Plant Pathology Journal
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• 제20권2호
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• pp.135-141
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• 2004

A filamentous virus was isolated from taro (Colocasia esculenta Schott) showing mosaic and chlorotic feather-ing symptoms in Chuncheon, Gangwon province in 2002. Based on ELISA, its appearance in electron microscope, serological relationships, and RT-PCR using specific primer and nucleotide sequence analysis of the CP gene, the isolated virus was identified as Dasheen mosaic virus (DsMV) and designated as Korean isolated (DsMV-Kr). DsMV was not serologically related to Zantedeschia mosaic virus (ZaMV), which has been reported to infect an Araceae plants. Since the coat protein revealed electrophoretic heterogeneity, about 42 kDa, 39 kDa and 31 kDa by SDS-PAGE, an improved purification method was established for the production of antisera against DsMV-Kr. The purification method used in this study may be effectively applied to the purification of other filamentous viruses.