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http://dx.doi.org/10.14480/JM.2015.13.1.79

Development of strain-specific SCAR marker for selection of Pleurotus eryngii strains with higher β-glucan  

Kim, Su Cheol (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
Kim, Hye Soo (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
Cho, Yong Un (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
Ryu, Jae-San (Eco-friendliness Research Department, Gyeongnam-do Agricultural Rearch and Extension Services)
Cho, Soo Jeong (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
Publication Information
Journal of Mushroom / v.13, no.1, 2015 , pp. 79-83 More about this Journal
Abstract
In this study, SCAR marker that differentiates Pleurotus eryngii strains with higher ${\beta}$-glucan from control strain was developed. Genomic DNAs of 9 control strains of Pleurotus eryngii and 9 Pleurotus eryngii strains with higher ${\beta}$-glucan were analyzed by bulked segregant analysis (BSA) using randomly amplified polymorphic DNA (RAPD). One-hundred twenty RAPD primers were screened on bulked DNA samples and a unique DNA fragment with the size of 91 bp was yielded by OP-R03 primer from the Pleurotus eryngii strains with higher ${\beta}$-glucan. A sequence characterized amplified region (SCAR) marker, designated as OP-R03-1-F and OP-R03-1-R, was designed on the basis of the determined sequence. The PCR analysis with the OP-R03-1 primer showed that this SCAR marker can clearly distinguish the Pleurotus eryngii strains with higher ${\beta}$-glucan from the control strains.
Keywords
Higher ${\beta}$-glucan; OP-R03-1 primer; Pleurotus eryngii; SCAR marker;
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Times Cited By KSCI : 5  (Citation Analysis)
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