Browse > Article
http://dx.doi.org/10.14480/JM.2014.12.3.226

Development of strain-specific SCAR marker for selection of Pleurotus eryngii strains adaptable to high-temperature  

Kim, Su Cheol (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
Kim, Hye Soo (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
Park, So Yeon (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
Ryu, Jae-San (Eco-friendliness Research Department, Gyeongnam-do Agricultural Rearch and Extension Services)
Cho, Soo Jeong (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
Publication Information
Journal of Mushroom / v.12, no.3, 2014 , pp. 226-231 More about this Journal
Abstract
In this study, SCAR marker that differentiates Pleurotus eryngii strains adaptable to high-temperature from control strain was developed. Genomic DNAs of 7 control strains of Pleurotus eryngii and 7 Pleurotus eryngii strains adaptable to high-temperature were analyzed by bulked segregant analysis (BSA) using randomly amplified polymorphic DNA (RAPD). Onehundred twenty RAPD primers were screened on bulked DNA samples and a unique DNA fragment with the size of 385 bp was yielded by OP-A06 primer from the Pleurotus eryngii strains adaptable to high-temperature. A sequence characterized amplified region (SCAR) marker, designated as OP-A06-1-F and OP-A06-1-R, was designed on the basis of the determined sequence. The PCR analysis with the OP-A06-1 primer showed that this SCAR marker can clearly distinguish the Pleurotus eryngii strains adaptable to high-temperature from the control strains.
Keywords
Pleurotus eryngii; High-temperature adaptable strains; SCAR marker; OP-A06-1 primer;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 Kim HK, Cheong JC, Seok SJ, Kim GP, Cha DY, Moon BJ. 1997. The artificial cultivation of Pleurotus eryngii (II). Kor. J. Mycol. 25:311-319.   과학기술학회마을
2 Kim SC, Hwang HS, Cho YJ, Kim HS, Ryu JS, Cho SJ. 2013. Development of a psychrophilic-SCAR marker for Pleurotus eryngii. J. Mushroom Sci. Prod. 11:171-176.(in Korean)   과학기술학회마을   DOI
3 Koveza OV, Gostimsky SA. 2005. Development and study of SCAR marker in pea (Pisum sativum L.). Russian. J. of Genetics. 15:34-35.
4 Lee DJ, Kim KP, Lee BE. 2003. Studies on artificial cultivation of Pleurotus eryngii (De Canolle ex Fries) Quel. Kor. J. Mycol. 15:34-35.
5 Paran I, Michelmore RW. 1993. Development of reliable PCR-based, markers linked to downy mildew resistance genes in lettuce. Theor. Appl. Genet. 85:985-993.
6 Lee MY, Doh EJ, Park CH, Kim YH, Kim ES, Ko BS, Oh SE. 2006. Development of SCAR marker discrimination of Artemisia princeps and Artemisia argyi from other Artemisia Herbs. Biol. Pharm. Bull. 29:629-633.   DOI   ScienceOn
7 Panner GA, Bush A, Wise R, Kim W, Domier L, Kasha K, Laroche A, Scoles G, Molnar SJ, Fedak G. 1993. Reproducibility of random amplified polymorphic DNA(RADP) analysis among laboratories. Genome Res. 2:341-345.   DOI
8 Paran I, Michelmore RW. 1991. Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc. Natl. Acad. Sci. USA. 88:9828-9832.   DOI   ScienceOn
9 Qin LH, Tan QI, Chen MJ, Pan YJ. 2006. Use of intersimple sequence repeats markers to develop strainspecific SCAR markers for Lentinula edodes. FEMS Microbiol. Lett. 257:112-116.   DOI   ScienceOn
10 Bang KH, Sung JS, Park CH, Jin DC, Park CG, Yu HS, Park HW, Seong NS. 2004. Discrimination of atractylodes rhizome white using anatomical characteristics and SCAR Markers. Kor. J. Medicinal Crop Science. 7:53-59.   과학기술학회마을
11 Hashimoto K, Takahashi Z. 1974. Studies on the growth of Pleurotus ostreatus. Mushroom Science. 9:585-593.
12 Hongyan S, Lei W, Yihe G, Ermei F, Jie S, Linde L. 2008. Development of strain-specific SCAR markers for authentication of Ganoderma lucidum. World J. Microbiol. Biotechnol. 24:1223-1226.   DOI
13 Kim MK, Ryu JS, Yoo YB. 2011. Characterization of a new cultivar "Dan Bi" by mono-mono hybridization in Pleurotus eryngii. Kor. J. Mycol. 39:39-43.   과학기술학회마을   DOI   ScienceOn
14 Rajarathnam R, Bano Z. 1987. Pleurotus mushrooms. Part 1A. Morphology, Lifecycle, Tanxonomy. Breeding and cultivation. CRC Critical in Food Science and Nutrition. 26:157-222.   DOI   ScienceOn
15 Ryu JS, Kim MK, Kwon JH, Cho SH, Kim NK, Rho CW, Lee CH, Ro HS, Lee HS. 2007. The growth characteristics of Pleurotus eryngii. Kor. J. Mycol. 35:47-53.   과학기술학회마을   DOI   ScienceOn
16 Seo KI, Jang KY, Yoo YB, Park SY, Kim KH, Kong WS. 2011. Development of suhan strain-specific SCAR marker in Pleurotus ostreatus. Kor. J. Mycol. 39:31-38.   과학기술학회마을   DOI   ScienceOn
17 Song YJ, Jeong MJ, Kim BG, Rho YD, Ryu JC, Yoo YB. 1996. Genetic variability of Pleurotus ostreatus monospore isolates by random amplified polymorphic DNA analysis. Kor. J. Mycol. 24:186-205.   과학기술학회마을
18 Zadrazil F. 1974. The ecology and industrial production of Pleurotus ostreatus, Pleurotus folrida, Pleurotus cornucopiae and Pleurotus eryngii. Mush. Sci. IX (Part 1):21-652.