• Parasites, Hosts and Diseases
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• 제52권6호
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• pp.613-619
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• 2014

Neospora caninum (Apicomplexa; Sarcocystidae) is a protozoan that causes abortion in cattle, horses, sheep, and dogs as well as neurological and dermatological diseases in dogs. In the central nervous system of dogs infected with N. caninum, cysts were detected that exhibited gliosis and meningitis. Flavonoids are polyphenolic compounds that exhibit antibacterial, antiparasitic, antifungal, and antiviral properties. In this study, we investigated the effects of flavonoids in a well-established in vitro model of N. caninum infection in glial cell cultures. Glial cells were treated individually with 10 different flavonoids, and a subset of cultures was also infected with the NC-1 strain of N. caninum. All of the flavonoids tested induced an increase in the metabolism of glial cells and many of them increased nitrite levels in cultures infected with NC-1 compared to controls and uninfected cultures. Among the flavonoids tested, 3',4'-dihydroxyflavone, 3',4',5,7-tetrahydroxyflavone (luteolin), and 3,3',4',5,6-pentahydroxyflavone (quercetin), also inhibited parasitophorous vacuole formation. Taken together, our findings show that flavonoids modulate glial cell responses, increase NO secretion, and interfere with N. caninum infection and proliferation.

• Journal of Ginseng Research
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• 제28권2호
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• pp.120-126
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• 2004

수천 년 동안 전통적 약제로 사용되어온 고려인삼은 중추신경계의 항상성을 유지하고 면역기능을 강화하는 효능을 나타내는 것으로 알려져 있다. 신경계질환의 진행이 대부분 염증 또는 면역반응을 수반하며, 이로 인하여 손상된 신경세포의 수복과정에 교세포 기원의 매개물질들이 기여하므로, 교세포에서의 cytokine 및 NO생성에 대한 연구는 신경기능과 신경면역기능의 조절 뿐 아니라 신경계 질환에 대한 연구의 초석이라고 할 수 있다. 따라서 본 연구에서는 고려인삼의 신경면역 및 염증반응 조절효과에 대하여 연구하고자 하였으며, 이를 위하여 흰쥐 대뇌피질의 교세포를 일차배양하며 고려인삼사포닌 분획을 처치하여 TNF-$\alpha$, IL-1$\beta$, 및 NO의 생성변동을 연구하였다. 백삼 사포닌은 50-500$\mu\textrm{g}$/ml 용량에서 TNF-$\alpha$와 IL-1$\beta$ 생성을 증가시켰으며, 미세교세포에서 iNOS 발현 및 NO생성을 유도하였고 성상세포의 stellation을 초래하였다. 특히 백삼사포닌 50-100 $\mu\textrm{g}$/ml는 세포독성을 거의 나타내지 않았으므로 이들 용량에 의한 교세포의 적절한 활성화가 중추신경계 면역기능 증가 및 염증반응 조절에 기여할 것으로 생각된다.

• 대한의생명과학회지
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• 제23권3호
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• pp.238-250
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• 2017

Patient's primary tumor-derived tumor cell lines likely represent ideal tools for human tumor biology in vitro and in vivo. Here, we describe eight human gastric carcinoma cell lines derived from established tumors in vivo upon subcutaneous transplantation of primary gastric carcinoma specimens in BALB/c nude mice. These xenografted gastric tumor cell lines (GTX) displayed close similarity with primary gastric tumor tissues in their in vivo growth pattern and genomic alterations. GTX-085 cells were resistant to cisplatin, while GTX-087 was the most sensitive cell line. GTX-085 was the only cell line showing a metastatic potential. Epithelial cell adhesion molecule (EPCAM) expression was especially strong in all tissue samples, as well as in cell cultures. GTX-139, the largest tumor graft obtained after injection, displayed distinct expression of CD44v6, fibroblast growth factor receptor 2 (FGFR2), and prominin 1 (PROM1, also known as CD133). In summary, we established eight xenograft gastric cancer cell lines from gastric cancer patient tissues, with their histological and molecular features consistent with those of the primary tumors. The established GTX cell lines will enable future studies of their responses to various treatments for gastric cancer.

• 식물조직배양학회지
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• 제27권2호
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• pp.149-154
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• 2000

땅두릅 미성숙 화기절편을 이용하여 1 mg/L 2,4-D의 고체배지에서 배발생 캘러스를 유도하였고 동일조건의 액체배지에서 증식시켰다. 배발생 세포를 270$\mu\textrm{m}$의 걸름체로 거른 다음 배발생 세포는 싸이토카이닌이 포함된 MS 액체배지에서 2주 동안 배양하였고 그후 생장조절물질이 없는 MS 액체배지에서 5주간 배양하여 1차 체세포배가 유도되었다. 이들중 어뢰형과 자엽형 시기의 배를 생장조절물질이 없는 MS고체 배지에 치상하였을 때 캘러스의 형성 없이 2차배가 직접 유도되었다. 2차배를 유도할 때 2mg/L kinetin에서 얻어진 1차 배에서 가장 높은 빈도의 2차배가 발생되었다. 식물체 재생은 1 mg/L kinetin 혹은 1 mg/L zeatin을 처리하여 얻어진 1차 자엽형배로부터 유도된 2차 자엽형배를 생장조절물질이 없는 MS 고체배지에 치상하였을 때 높게 나타났다 (각각 25.4%, 28.6%). 2차배로부터의 3차배 발생과 식물체 재생과의 상관 관계를 볼 때 식물체의 재생에 있어서 3차배의 배발생은 저해적인 영향을 주었다.

• Fisheries and Aquatic Sciences
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• 제4권4호
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• pp.186-191
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• 2001

Vitellogenin (VTG) and VTG mRNA induction by $estradiol-17\beta\;(E_2)$ were examined in the primary cultures of hepatocyte in the rainbow trout. Hepatocytes were precultured for 2 days, then $E_2$ was added and cultured for another 5 days. Media and hepatocytes were then analyzed by electrophoresis and Northern blotting for VTG and VTG mRNA, respectively. The hepatocytes were formed a few aggregates within 5 days without further spreading to a monolayer. Cell viability and high DNA content were maintained during the incubation. The hepatocyte culture with E2 induced a weak VTG band at a molecular weight of 175kDa on Day 2 after $E_2$ addition. The relative amount of VTG was expressed in percentage of total protein concentrations. VTG was gradually increased as $1.9\%$ on Day 2, $6.3\%$ on Day 4 and $7.3\%$ on Day 5. VTG mRNA band was detected at about 6.6 kb in the culture with $E_2$ at day 1 of culture. The level of VTG mRNA expression linearly increased with time until Day 5 (r=0.97).

• 약학회지
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• 제46권6호
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• pp.466-471
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• 2002

Oxidative stress is considered to be associated with many diseases, such as inflammatory and cardiovascular diseases, aging and cancer. An important etiological mechanism of these diseases may be a causal relationship between the presence of oxidants and the generation of lipid hydroperoxides derived from enzymatic reactions or xenobiotic metabolism. The hydroperoxides can be decomposed to alkoxy- (ROㆍ) and peroxy- (ROOㆍ) free radicals that can oxidize other cell components, resulting in changes in enzyme activity or the generation of mediators, which can cause further cell damage. The aim of this study was to evaluate the ability of aqueous extract from the roots of Platycodon grandiflorum A. DC (Campanulaceae), Changkil (CK), to affect cellular response in primary cultures of rat hepatocytes to t-butyl hydroperoxide (t-BHP) induced oxidative stress and hepatotoxicity. CK-treated cells showed an increased resistance to oxidative challenge, as revealed by a higher percent of survival capacity in respect to control cells. CK reduced t-BHP-enhanced lipid peroxidation measured as production of malondialdehyde and enhanced intracellular reduced glutathione depletion by t-BHP. Furthermore, CK protected from the t-BHP-induced intracellular generation of reactive oxygen species assessed by monitoring dichlorodihydrofluorescein fluorescence. It can be concluded that CK exerts an antioxidant action inside the cell, responsible for the observed modulation of the cellular response to oxidative challenge, and CK have a marked antioxidative and hepatoprotective potency.

• 동의신경정신과학회지
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• 제10권2호
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• pp.59-70
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• 1999

We investigated whether an aqueous extract of Rehmannia glutinosa steamed root (RGAE) inhibits secretion of inflammatory cytolanes from primary cultures of mouse astrocytes. RGAE dose-dependently inhibited the $TNF-{\alpha}$ secretion by astrocytes stimulated with substance P (SP) and lipopolysaccharide (LPS). Interleukin-1 (IL-1) has been shown to elevate $TNF-{\alpha}$ secretion from LPS-stimulated astrocytes while having no effect on astrocytes in the absence of LPS. We therefore also investigated whether IL-1 mediated inhibition of $TNF-{\alpha}$ secretion from primary astrocytes by RGAE. Treatment of RGAE to astrocytes stimulated with both LPS and SP decreased IL-1 secretion to the level observed with LPS alone. Moreover, incubation of astrocytes with IL-1 antibody abolished the synergistic cooperative effect of LPS and SP. These results suggest that RGAE has an antiinflammatory activity on the central nervous system curing some pathological disease states.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2002년도 생물공학의 동향 (X)
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• pp.305-308
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• 2002

접종 초기 root segmentation 은 뿌리의 생장 속도를 감소시키고 그 생산성을 저하시키기도 하지만 F/D ratio를 감소시켜 뿌리가 자랄 수 있는 배지 내 공간을 제공하기 때문에 고농도 배양에 유리할 뿐 아니라, 생산성이 높은 secondary root의 비율을 증가시키기 때문에 decursinol angelate 의 생산량을 중대시킨다. 참당귀 뿌리배양을 위한 plant growth regulator의 최적 조건으로 secondary root의 생장성 측면이나 decursinol angelate 의 생산성 측면에서 4 mg/L 이상의 IBA 첨가가 요구됨을 확인하였다.

• 약학회지
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• 제46권3호
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• pp.185-191
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• 2002

Alzheimer's disease (AD) involves neuronal degeneration with impaired cholinergic transmission, particularly in areas of the brain associated with learning and memory. Several cholinesterase inhibitors are widely prescribed to ameliorate the cognitive deficits in AD patients. In an attempt to examine if tacrine and donepezil, two well-known cholinesterase inhibitors, exhibit additional pharmacological actions in primary cultured rat cortical cells, we investigated the effects on neuronal injuries induced by glutamate or N-methyl-D-aspartate (NMDA), $\beta$-amyloid fragment ( $A_{{beta}25-35)}$), and various oxidative insults. Both tacrine and donepezil did not significantly inhibit the excitotoxic neuronal damage induced by glutamate. However, tacrine inhibited the toxicity induced by NMDA in a concentration-dependent fashion. In addition, tacrine significantly inhibited the $A_{{beta}25-35)}$-induced neuronal injury at the concentration of 50 $\mu$M. In contrast, donepezil did not reduce the NMDA- nor $A_{{beta}25-35)}$-induced neuronal injury. Tacrine and donepezil had no effects on oxidative neuronal injuries in cultures nor on lipid peroxidation in vitro. These results suggest that, in addition to its anticholinesterase activity, the neuroprotective effects by tacrine against the NMDA- and $A_{{beta}25-35)$-induced toxicity may be beneficial for the treatment of AD. In contrast, the potent and selective inhibition of central acetylcholinesterase appears to be the major action mechanism of donepezil.

• 가족자원경영과 정책
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• 제12권4호
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• pp.63-77
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• 2008

This study was performed to develop the basic framework of multicultural art education programs for female foreign immigrants married to Korean males. First, these programs aim to lessen their cultural variance in acculturizing in Korea, and thereafter enhance their emotional stability and improve their creative ability to integrate cultural diversity into family life. The primary objective of this study was to offer a suitable education program and evaluation criteria for the program; the research has been done through person-to person interviews with 53 subjects using a series of questionnaires composed of 8 five-point Likert-Scaled items. As for the process of the interview, the subjects were asked about their satisfaction with each program item twice in order to comparetheir impressions before and after program participation. The satisfaction scores were analyzed using frequency distribution. In addition, the program effects were also evaluated by coordinators and developers from their subjective comments through periodical evaluation meetings. From the results, it is observed that art education programs are beneficial for improving female immigrants' emotionaland cultural integration. In addition, experts determined that the programs were helpful for improving their human relationship and acculturization in Korea. Finally, it is concluded that multicultural education programs are a primary fundamental infra-structural way of integrating diverse cultures and improving communication exchanges, and therefore, improve family stability among the multicultural families in Korea.