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http://dx.doi.org/10.3347/kjp.2014.52.6.613

Flavonoids Modulate the Proliferation of Neospora caninum in Glial Cell Primary Cultures  

Matos, Rosan Barbosa De (Laboratorio de Neuroquimica e Biologia Celular, Instituto de Ciencias da Saude, Universidade Federal da Bahia - UFBA)
Braga-de-Souza, Suzana (Laboratorio de Neuroquimica e Biologia Celular, Instituto de Ciencias da Saude, Universidade Federal da Bahia - UFBA)
Pitanga, Bruno Pena Seara (Laboratorio de Neuroquimica e Biologia Celular, Instituto de Ciencias da Saude, Universidade Federal da Bahia - UFBA)
Silva, Victor Diogenes Amaral Da (Laboratorio de Neuroquimica e Biologia Celular, Instituto de Ciencias da Saude, Universidade Federal da Bahia - UFBA)
Jesus, Erica Etelvina Viana De (Laboratorio de Neuroquimica e Biologia Celular, Instituto de Ciencias da Saude, Universidade Federal da Bahia - UFBA)
Pinheiro, Alexandre Morales (Laboratorio de Bioquimica e Imunologia Veterinaria, Universidade Federal do Reconcavo da Bahia, Centro de Ciencias Agrarias Ambientais e Biologicas, Campus da Universidade)
Costa, Maria De Fatima Dias (Laboratorio de Neuroquimica e Biologia Celular, Instituto de Ciencias da Saude, Universidade Federal da Bahia - UFBA)
El-Bacha, Ramon Dos Santos (Laboratorio de Neuroquimica e Biologia Celular, Instituto de Ciencias da Saude, Universidade Federal da Bahia - UFBA)
Ribeiro, Catia Suse De Oliveira (Laboratorio de Neuroquimica e Biologia Celular, Instituto de Ciencias da Saude, Universidade Federal da Bahia - UFBA)
Costa, Silvia Lima (Laboratorio de Neuroquimica e Biologia Celular, Instituto de Ciencias da Saude, Universidade Federal da Bahia - UFBA)
Publication Information
Parasites, Hosts and Diseases / v.52, no.6, 2014 , pp. 613-619 More about this Journal
Abstract
Neospora caninum (Apicomplexa; Sarcocystidae) is a protozoan that causes abortion in cattle, horses, sheep, and dogs as well as neurological and dermatological diseases in dogs. In the central nervous system of dogs infected with N. caninum, cysts were detected that exhibited gliosis and meningitis. Flavonoids are polyphenolic compounds that exhibit antibacterial, antiparasitic, antifungal, and antiviral properties. In this study, we investigated the effects of flavonoids in a well-established in vitro model of N. caninum infection in glial cell cultures. Glial cells were treated individually with 10 different flavonoids, and a subset of cultures was also infected with the NC-1 strain of N. caninum. All of the flavonoids tested induced an increase in the metabolism of glial cells and many of them increased nitrite levels in cultures infected with NC-1 compared to controls and uninfected cultures. Among the flavonoids tested, 3',4'-dihydroxyflavone, 3',4',5,7-tetrahydroxyflavone (luteolin), and 3,3',4',5,6-pentahydroxyflavone (quercetin), also inhibited parasitophorous vacuole formation. Taken together, our findings show that flavonoids modulate glial cell responses, increase NO secretion, and interfere with N. caninum infection and proliferation.
Keywords
Neospora caninum; glia; flavonoid; nitric oxide (NO);
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