• Preventive Nutrition and Food Science
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• 제9권3호
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• pp.206-212
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• 2004

Prickly pear extract (PPE) was fermented by Lactobacillus rhamnosus LS at 3$0^{\circ}C$ for 2 days. To improve the physicochemical properties of fermented PPE, it was fortified with food polysaccharides (0.2 %) or seaweed calcium before lactic acid fermentation. The viable cell counts, flow behavior, titratable acidity and color stability of fermented PPE were evaluated during 4 weeks of cold storage. Addition of xanthan gum or glucomannan increased the apparent viscosity and acid production, viable cell counts and red color of PPE were also well maintained during the cold storage. However, fermenting PPE with gellan gum resulted in a decrease in relative absorbance, indicating lower color stability. In particular, PPE fortified with carrageenan or alginic acid showed reduced acid production and lower viable cell counts. Addition of seaweed calcium at a 0.1 % level had positive effects on color stability, and helped maintain viable cell counts of 4.1 ${\times}$ 10$^{9}$ CFU/mL. This study demonstrated that xanthan gum could be used as a good thickening agent and stabilizer for retaining viable cell counts and red color during the cold storage in PPE fermented by lactic acid bacteria.

• Preventive Nutrition and Food Science
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• 제9권1호
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• pp.7-13
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• 2004

Lactic acid fermentation of prickly pear extract (PPE) was performed by Lactobacillus rhamnosus LS, Lactobacillus bulgaricus, and Lactobacillus brevis. The PPE was pasteurized to eliminate indigenous microorganisms as well as to dissolve the partially insoluble pulp. The PPE fermented without yeast extract by L. rhamnosus LS exhibited 0.57％ acidity and 3.5${\times}$10$^{8}$ CFU/mL bacteria count. With the addition of 0.2% edible yeast extract the PPE fermented by L. rhamnosus LS exhibited 1.15％ acidity,2.7${\times}$10$^{9}$ CFU/mL bacteria count and 95.0% retention of red color. When 5％ fructose syrup was added, the PPE fermented by L. rhamnosus LS had 1.09% acidity, 6.5${\times}$10$^{8}$ CFU/mL, and 97.7% retention of red color. With 1∼3% (w/v) concentrations of starter, the PPE fermented by L. bulgaricus and L. brevis showed 0.97% and 0.65% acidities, respectively. The viable cell counts from L. rhamnosus LS fermentation were higher compared with those of other LAB. During cold storage at 4$^{\circ}C$, the viable cell count was well maintained for 3 weeks, but then rapidly decreased. The red pigment was highly stable during cold storage for 4 weeks. The pasteurized PPE fortified with 5% fructose syrup, 0.2% yeast extract, and 0.05% CaCO$_3$ was successfully fermented by inoculating with 3% LAB and incubating at 3$0^{\circ}C$ for 2 days. Both viable cell counts and the red color of the fermented PPE were well maintained during cold storage for 3 weeks.

• 생약학회지
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• 제33권3호통권130호
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• pp.230-237
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• 2002

Prickly pear(Opuntia ficus-indica var saboten Makino, Cactaceae) is widely used as folk medicine for burned wound, edema and indigestion. To confirm the hypolipidemic action of the extract from Cactus stem and fruit in dietary hyperlipidemic rats, variations of the lipid- or cholesterol-related components were investigated. As results these changes were significantly prevented by treatments with Cactus extracts. The effects of the extract of fruit was better than those of stem.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1998년도 Proceedings of UNESCO-internetwork Cooperative Regional Seminar and Workshop on Bioassay Guided Isolation of Bioactive Substances from Natural Products and Microbial Products
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• pp.187-187
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• 1998

The objective was to verify biological activities of fruit and stem of prickly pear(Opuntia ficus indica L. var, saboten Makino). We have determined inhibitory activities on enzymes, such as dopamine ${\beta}$-hydroxylase(DBH), monoamme oxidase A and B(MAO-A, B), and antioxidant activity, in vitro. We purchased dried stem powder and lyophilized fruit powder of prickly pear from CheJu Island, and prepared the extracts with 80% of methanol. The fruit extract showed stronger inhibitory effects on MAO-A and -B and antioxidant activity compared. to the stem extract, on fractionation with hexane, ethyl acetate, butanol and water. Both the stem and the fruit extracts with ethyl acetate showed stronger enzyme inhibitory and antioxidant activities than other extracts. Now we are isolating active principles from both ethyl acetate extracts.

• 식품산업과 영양
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• 제9권2호
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• pp.53-59
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• 2004

• 한국영양학회:학술대회논문집
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• 한국영양학회 2003년도 연합학술대회
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• pp.229.2-229.2
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• 2003

• 한국식품영양과학회지
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• 제34권3호
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• pp.408-413
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• 2005

Glucomannan과 κ-carrageenan을 혼합하여 액상과당, 해조칼슘, 유기산, 백년초 열매 추출액의 젖산 발효액을 첨가한 젤리를 제조하였다. 백년초 열매 추출액의 젖산발효액은 pH 3.12, 적성산도 1.15%(lactic acid, v/v), 2.7×10/sup 9/CFU/mL의 젖산균을 포함하며 L값 19.6, a값 12.3, b값 1.4를 나타내었다. 젤리 제조시에 백년초 발효액을 4%첨가하였을 때, 견고성은 약간 낮지만 탄력성이 높은 부드러운 젤리의 제조가 가능하였고, 젤리의 색과 맛에서도 좋은 기호도를 나타내었다. 유기산 중 citric acid를 0.25%(v/v) 첨가했을 때 견고성과 탄력성이 좋은 젤리를 형성한 반면, ascorbic acid를 0.25%(v/v) 첨가했을 때는 푸딩과 같은 부드러운 젤리가 형성되었다. κ-carageenan농도에 따른 젤리의 제조에서 0.75%의 κ-carrageenan을 첨가했을 때 젤리의 견고성과 brittleness가 높았고, 0.9% 첨가시에는 오히려 젤리의 견고성이 감소하였다. 관능검사 결과, 0.25%의 citric acid와 30%의 액상과당을 함유하며 0.15%의 glucomannan과 0.6% 또는 0.75%의 κ-carrageenan을 혼합한 젤리의 전반적인 선호도가 높았다.

• 한국식품영양과학회지
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• 제27권5호
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• pp.821-826
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• 1998

Functional properties of mucilage and pigment extracted from Opuntia ficus-indica var. saboten were determined at various temperatures, pHs and alcohol concentrations. The crude mucilage extracted from pickly pear showed pH 4.2, 0.14% total acidity and 8.1% total soluble solid content(w/w, wet basis). Polysaccharide was purified from mucilage extract by isopropanol precipitation. Intrinsic viscosity of polysaccharide was 18.1dl/g and decreased with increasing KCl concentration. Relative viscosity and color stability of mucilage extract were determined with capillary viscometer and spectrophotometer at 534nm, respectively. In additions of 1~20%(v/v) ethanol, the red pigment of mucilage extract was very stable, but relative viscosity, increased gradually. For heating above 7$0^{\circ}C$, the stability of red pigment decreased drastically, but rheological property of mucilage was not changed. During storage, the red pigment was extremely unstable at above pH 8.3. At both pH 3.0 and pH 4.2, the red pigment was the most stable at 4$^{\circ}C$ for 18 days. In the case of storage at 37$^{\circ}C$, pigment of mucilage extract at pH 3.0 was destroyed more quickly than that at pH 4.2. Natural mucilage extract(pH 4.2) showed the good stability of red pigment at 3$0^{\circ}C$ for 10 days.

• 한국식품저장유통학회지
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• 제16권6호
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• pp.953-958
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• 2009

신안산 손바닥선인장 butanol 추출물과 methanol 추출물이 10균주 중 세균에 대하여 항균활성이 강하였으나, 젖산균 및 효모에 대해서는 항균활성이 나타나지 않았다. 부위별 항균활성은 줄기 추출물이 열매 추출물 보다 강한 활성을 나타내었다. 손바닥선인장의 butanol 추출물의 최소저해 농도는 세균은 100 mg/mL 이었고, 효모와 젖산균은 500 mg/mL이었다. 손바닥선인장 추출물의 열 안정성은 $100^{\circ}C$에서 30분 가열시에도 항균활성이 거의 감소되지 않아 열에 안정한 물질로 확인 되었다. 손바닥선인장 butanol 추출물에 함유된 항균활성 물질의 pH 안정성은 산성인 pH 3과 알칼리인 pH 9 에서도 항균활성이 감소되지 않아 산과 알칼리에서도 안정한 물질로 확인되었다. 손바닥선인장의 추출물별 DPPH free radical scavenging activity는 열매와 줄기 모두 water, methanol, butanol, ethyl-acetate 및 diethylether 순으로 나타냈고, 시료 부위별로는 줄기가 열매에 비해 항산화성 활성이 높게 나타냈다. 손바닥선인장의 폴리페놀함량은 열매가 520.57 mg%로 줄기 203.26 mg% 보다 2배정도 높은 함량을 나타내었다.

• 생약학회지
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• 제43권4호
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• pp.308-315
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• 2012

Opuntia humifusa known as the Eastern prickly pear have been used as a treatment of burns, diarrhea, asthma, rheumatism, gonorrhea, and diabetes in alternative medicine. O. humifusa is widely cultivated in the middle and southern provinces of Korea and distributed in North America. The aim of this study is to investigate anti-diabetic effect of O. humifusa stem (OHS) water or 80% MeOH extract using 3T3-L1 adipocytes and db/db mice animal models. OHS 80% MeOH extract at a dose of $250{\mu}g/ml$ significantly increased the glucose uptake and lipid accumulation compared with the control in 3T3-L1 adipocytes. Blood glucose, plasma total cholesterol and triglyceride levels were significantly reduced by oral treatment of OHS 80% MeOH extract (200 mg/kg BW) for 6 weeks in db/db mice. Also, the oral treatment of OHS 80% MeOH extract slightly changed the plasma insulin and insulin resistance levels in db/db mice, but were no significance in comparison to control. Glucose transporter(GLUT)4 expressions of adipose tissue and muscle were significantly increased more than that in the control. Therefore, these results suggest that OHS 80% MeOH extract inhibits the blood glucose level through regulation of lipid profile, insulin resistance, and GLUT4 expression in db/db mice and its diabetic effect is effective more than water extract.