Biological Activities of the Extracts from Fruit and Stem of Prickly Pear(Opuntia ficus-indica var. saboten) II.-Effects on Dietary induced Hyperlipidemia

손바닥선인장 열매 및 줄기 추출물의 생리활성(II)-흰쥐의 식이성 고지혈증에 미치는 영향

  • 이정규 (경성대학교 약학대학) ;
  • 이영철 (한국식품개발연구원 농산물이용연구부) ;
  • 문영인 (북제주군 농업기술센터) ;
  • 박희준 (상지대학교 응용식물과학부) ;
  • 한용남 (서울대학교 천연물과학연구소) ;
  • 최종원 (경성대학교 약학대학)
  • Published : 2002.09.30

Abstract

Prickly pear(Opuntia ficus-indica var saboten Makino, Cactaceae) is widely used as folk medicine for burned wound, edema and indigestion. To confirm the hypolipidemic action of the extract from Cactus stem and fruit in dietary hyperlipidemic rats, variations of the lipid- or cholesterol-related components were investigated. As results these changes were significantly prevented by treatments with Cactus extracts. The effects of the extract of fruit was better than those of stem.

Keywords

References

  1. 보건복지부(1997) 1997년도 국민 영양 조사 결과서. p.37, 문영사, 서울
  2. Sakai, Y, Nagase, H., Ose, Y, Sato, T, Kawai, M. and Mizuno, M. (1988) Effect of medicinal plant extracts from Chinese herbal medicines on the mutagenic activity of benzo[$\alpha$]Pyrene. Mutat. Res., 206(3): 327 https://doi.org/10.1016/0165-1218(88)90118-8
  3. Cao, Z. L., Zhu, M., Liu, H. X. and Xiao, Z. M. (1988) Effects of Radix Rehmanniae and processed Radix Rehman-niae on some immune functions in mice. Chung Yao Tung Pao., 13(10): 22, 62
  4. Kubo, M., Asano, T., Shiomoto, H. and Matsuda, H. (1994)Studies on Rehmanniae Radix. I. Effect of 50% ethanolic extract from steamed and dried Rehmanniae Radix on hemorheology in arthritic and thrombosic rats. Biol. Pharm. Bull., 17(9): 1282 https://doi.org/10.1248/bpb.17.1282
  5. Kitagawa, I., Fukuda, Y, Taniyama, T. and Yoshikawa, M. (1995) Studies on crude drug processing. X. On the constituents of Rehmanniae Radix(4): comparison of the constituents of various Rehmanniae Radixes originating in China, Korea and Japan., Yakugaku Zasshi., 115(12): 992 https://doi.org/10.1248/yakushi1947.115.12_992
  6. Kubo M., Asano T., Matsuda H., Yutani S. and Honda S. (1996) Studies on Rehmanniae Radix. Ⅲ. The reladon between changes of consdtuents and improvable effects on hemorheology with the processing of roots of Rehmannia glutinosa. Yakugaku Zasshi., 116(2): 158 https://doi.org/10.1248/yakushi1947.116.2_158
  7. 최종원, 이정규, 이영철, 문영인, 박희준, 한용남(2001) 손바닥선인장 열매 및 줄기 추출물의 생리활성(I)-일반약리검색, 생약학회지, 32(4): 330-337
  8. Han, Y. N., Baik, S. K., Kim, T. H. and Han, B. H. (1987) Antithrombotic acdvides of saponins from Ilex pubescens. Arch. Pharm. Res., 10: 115 https://doi.org/10.1007/BF02857777
  9. Quick, A. J., Stanley-Brown, M. and Bancroft, F. W. (1935) A study of the coagluadon defect in hemophilia and in jaundice. Am. J. Med. Sci. 196: 571
  10. McGowan, M. W., Artiss, J. D. and Strandbergh, D. R. (1983) A peroxidase-coupled method for the colorimetric determination of serum triglycerides. Clin. Chem., 29: 538
  11. Chen, P. S., Toribara, T. Y. and Warnerm, H. (1959) Micro determination of phosphorus. Anal. Chem., 28: 1756
  12. Frings, C. S. and Dunn, R. J. (1970) A colorimetric method of determination of total serum lipids based on the sulfophosphovanillin reaction. J. Chem. Path., 53: 89
  13. Richmond, W. (1976) Use of cholesterol oxidase for assay of total and free cholesterol in serum by condnuous flow analysis. Clin. Chem., 22: 1579
  14. Noma, A., Nakayama, K. N., Kota, M. and Okabe, H. (1978) Simultaneous determination of serum cholesterol in high and low density lipoprotein with use of heparin, $Ca^{2+}$ and an anion exchange resin. Clin. Chem., 24: 1504
  15. Noma, A., Okabe, H., Nakayama, K. N., Ueno, Y. and Shinohara, H. (1979) Improved method for simultaneous determination of cholesterol in high- and low-density lipoproteins. Clin. Chem., 25: 1480
  16. Fridewald, W. T, Levy, R. I. and Fedreicson, D. S. (1979) Estimation of concentration of low density lipoprotein cholesterol in plasma without use of the preparative ultracentrifuge. Clin. Chem., 18: 499
  17. Haglund, O., Luostaiinen, R., Wallin, R., Wibell, L. and Saldeen, T. (1991) The effects of fish oil on triglycende, cholesterol, fibdnogen and malon-dialdehyde in humans supplemented with vitamin E. J. Nutr., 121: 165
  18. Tietz, M. W. and Fiereck, E. A. (1966) A specific method for serum lipase determination. Clin. Chem. Acta, 13: 352 https://doi.org/10.1016/0009-8981(66)90215-4
  19. Yagi, K. (1987) Lipid peroxides and human diseases. Chemistry and Physics of Lipid, 45: 337 https://doi.org/10.1016/0009-3084(87)90071-5
  20. Kobatake, Y, Saito, M., Kuroda, K. Kobayashi, S. and Innami, S. (1987) Influence of fish consumption on serum lipid and lipid peroxide concentrations in middle aged subjects. J. Japan Soc. Nutr. & Food Sci., 40: 103 https://doi.org/10.4327/jsnfs.40.103
  21. Oyanagui, Y. (1984) Reevaluation of assay methods and establishment of kit for superoxide dismutase activity. Anal. Biochem., 42: 290
  22. Folch, J., Lees, M. and Stanley, G.H. (1957) A simple method for the isolation and perification of total lipids from animal tissues. J. Biol. Chem., 226: 497
  23. Ichida, T. (1963) The effect of cholesterol-feeding on the tissue lipids. Hokkaido J. Med. Sci., 38: 67
  24. Barma, L. and Triplett, D.A.(1989) Use of the activated partial thromboplastin time for the diagnosis of congenital coagulation disorders: Ploblems and possible solutions, Ric.Clin. Lab., 19(4): 345-354
  25. Surprenant, Y. M. and Zuckerman, S. H. (1989) A novel microtiter plate assay for the quandtation of procoagulant activity on adherent monocytes, macrophages and endothe- lial cells. Thromb. Res., 53: 339 https://doi.org/10.1016/0049-3848(89)90108-4
  26. Kleinsek, D. A., Dugan, R. E., Baker, T. A. and Porter, J. W. (1981) 3-Hydroxy-3-methy1g1utary1 CoA reductase from rat liver. Method in Enzymol, 71(Part C): 462
  27. Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Rendall, R. J. (1951) Protein Measurement with Folin phenol reagent. J. Biol. Chem., 193: 265
  28. Miller, N. E. (1978) The evidence for the antiatherogenidty of high density lipoprotein in man. Lipid, 13: 914 https://doi.org/10.1007/BF02533850
  29. Ross, R. (1986) The pathogenesis of atherosclerosis. New Engl. J. Med., 314: 488
  30. Blankenhom, D. H. (1989) Regression of atherosclerosis dietary and pharmacologic approach. Can. J. Cardiol., 5(4): 206
  31. Hayek, T., Masucci-Magoulas, L., Jiang, X., Walsh, A., Rubin, E- Breslow, J. L. and TaIl, A. R. (1995) Decreased early atherosclerotic lesions in hypertriglyceridemic mice expressing cholesteryl ester protein transgene. J. Clin. Invest., 96(4): 2071 https://doi.org/10.1172/JCI118255
  32. Rodwell, V. W., Nordstrom, J. L. and Mitschelen, J. J. (1976) Regulation of HMG-CoA reductase. Adv. Lipid Res., 14: 1
  33. Hicher, H. F. and Olsen, W. H. (1973) SlimpliSed spectro-photometric assay for microsomal HMG-CoA reductase measurement of Coenzyme A. J. Lipid Research, 14: 625
  34. Amlie, T., Lyberg, A., Kaplun, J. H. and Prydz, H. (1981) Thromboplastin activity of mouse peritoneal macrophages. Thrombosis Research, 24: 61 https://doi.org/10.1016/0049-3848(81)90032-3
  35. Tanaka, H., Andoh, K., Narahara, N., Uchiyama, T, Kubota, T., Takada, M., Kobayashi, N. and Maekawa, T. (1986) The leukocyte membrane and its contribution to thrombosis and haemostasis with special reference to tissue factor. Acta Haematol. Jpn., 49: 1583
  36. Lesnik, R, Rouis, M., Skarlatos, S., Kmth, H. S. and Chapman, M. J. (1992) Uptake of exogenous free cholesterol induces upregulation of tissue factor expression in human monocyte-derived macrophages. Proc. Natl. Acad. Sci., USA., 89: 10370 https://doi.org/10.1073/pnas.89.21.10370
  37. Weis, H. R., Pitas, R. E., Wilson, B. D. and Rodgers, G. M. (1991) Oxidized low-density lipoprotein increases cultured human endothelial cell tissue factor activity and reduces protein C activation. The FASEB Joumal., 5: 2459
  38. Wada, H., Kaneko, T., Wakita, Y, Minamikawa, K., Nagaya, S., Tamaki, S., Deguchi, K. and Shirakawa, S. (1994) Effect of lipoproteins on tissue factor activity and PAI-Ⅱ antigen in human monocytes and macrophages. Int. J. Cardiol., 47: S21 https://doi.org/10.1016/0167-5273(94)90322-0