• 제목/요약/키워드: pression formation

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Global Approaches to Identify Genes Involved during Infection Structure Formation in Rice Blast Fungus, Magnaporthe grisea

  • Park, Woo-Bong
    • The Plant Pathology Journal
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    • 제19권1호
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    • pp.34-42
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    • 2003
  • The ascomycete Magnaporthe grisea is a pathogen of rice blast and is known to form specialized infection structures called appressoria for successful infection into host cells. To understand the molecular mechanism underlying infection process, appressorium-related genes were identified through global approaches including EST sequencing, differential hybridization, and sup-pression subtractive hybridization. EST database was generated on >2,000 cDNA clones randomly selected from appressorium stage cDNA library. Large number of ESTs showed homology to known proteins possibly involved in infection-related cellular development (attachment, germination, appressorium formation, and colonization) of rice blast fungus. The 1051 ESTs showing significant homology to known genes were assigned to 11 functional categories. Differential hybridization and suppression subtractive hybridization were applied to identify genes showing an appressorium stage specific expression pattern. A number of genes were selected as up-regulated during appressorium formation compared with the vegetative growing stage. Clones from various cDNA libraries constructed in different developmental stages were arrayed on slide glass for further expression profiling study. functional characterization of genes identified from these global approaches may lead to a better understand-ing of the infection process of this devastating plant disease, and the development of novel ways to protect host plant.

의복 스타일에 따른 인상 형성에서의 순서효과 (The Order Effect on Impressions Formed by the Function of Clothing Style)

  • 김경원;탁혜령;고애란
    • 한국의류학회지
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    • 제28권7호
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    • pp.995-1006
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    • 2004
  • This study examines the effects of clothing style and the order effect on impression formation. The instrument of this study consisted of response scales and stimuli. Fifty-one items of 7-point semantic differential scales, 9 items of demographic traits, and 4 items of subjective evaluation scales were developed. Stimuli were color pictures of a model wearing each of two clothing styles(mannish style, feminine style). The sample include 56 male and female subjects in their twenties and thirties. The experimental design was within-subject design and the half of the sample responded to the mannish style first and the other half responded to the feminine style first. Responses to the semantic differential scales were factor analyzed, and seven factors were identified: intellectuality, sensibility, activity, modesty, competence, display, keenness. There were significant differences between mannish style and feminine style in impression of wearer's age, job, and physical attractiveness as well as wearer's intellectuality and sensibility. It was revealed that the first impression and second impression of wearer's intellectuality, sensibility, modesty, competence, display were differed by the function of the order of stimuli shown to the subjects. Both primacy effect and recency effect of order effect were confirmed, and especially negativity effect was influenced prominently on impression formation.

초현실주의 복식양식에 관한 연구 (A Study on the Surrealism in Fashion)

  • 곽미영
    • 복식
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    • 제26권
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    • pp.233-250
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    • 1995
  • The purpose of this research is to open new horizon in the plastic art centering the clothings, to examine the characteristics of the clothing style of Surrealism closely which is being lighted up again newly in the recent culture that is in a state of flux, and futher to contribute to the cre-ation of future clothes by analyzing Surrealism once again which is considered to have affected most in the aspects of idea and technique from the era of modernism to the era of post modern-ism, to understand and develop the relation of modern style of paintings and clothing which consist of many complicated and diverse elements. As for the method of research, after analyzing the formation and characteristics of painting style of Surrealism, painting style of Surrealism, painting style of Surrealism were classified largely into D paysement and Automatism, which were then reviewed. And the characteristics of fashion style of Surrealism were compared and analyzed with painting style of Surrealism, for this, fashion of Surrealism dur-ing recent five years from 1990 to 1994, among the fashion from Pr t- -Porter concentrated re-view was made for the ones made public at Paris-London Collections. By the result observed through this research, the trompe-l'oeil double image technique of many clothes created by E. Schiaparelli who was strongly influenced by Surrealism in 1930s were succeeded with its inspiration of Surrealism by the radical fashion designers recently and we were able to know that it is being created again by fresh senses. Followings are the discussions on homogenity of the characteristics of fashion style of Surreal-ism with the painting style of Surrealism Therefore if we analyze the characteristics of fashion style of Surrealism, Automatism ex-pression style which takes out only image borrowing the power of unconsciousness, instead of factual transposition, is forming the main cur-rent after 1990s. We can find the fashion style of this Surrealism appear persistently, entirely irrel-evant to the silhouettes of 20th century.

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병원력 차이를 보이는 두 곤충병원세균(Xenorhabdus nematophilus와 Staphylococcus gallinarum)의 면역저하 능력 비교 분석 (Comparative Analysis of Host Insect Immunodepression Induced by Two Entomopathogenic Bacteria, Xenorhabdus nematophilus and Staphylococcus gallinarum, with Differential Pathogenicities)

  • 박영진;김길호;김용균
    • 한국응용곤충학회지
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    • 제42권4호
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    • pp.353-360
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    • 2003
  • 곤충병원세균이 효과적으로 병원력을 발휘하는 데 대상 곤충의 면역저하가 요구될 수 있다. 본 연구는 대상곤충의 면역저하가 병원세균의 살충능력에 직접 연관된다는 가설을 세웠다. 곤충병원세균을 5령 누에(Bombyx mori)의 혈강에 접종하였을 때, X. nematophilus는 다른 곤충병원세균인 Staphyiococcus gallinarum 보다 1,200배 높은 병원력을 보였다. 비록, 두 곤충병원세균은 누에의 혈구세포에 대해서 독성효과를 가지고 있지만, X. nematophiius는 S. gallinarum과 비교해보다 빠르고 높은 세포독성 영향을 보였다. 세포성 면역반응에서 5${\times}$$10^{5}$농도로 세균이 접종되었을 때, 누에는 약 20개 정도의 소낭을 형성하였다. 특히, 살아있는 X. nematophilus가 접종된 누에에서 소낭 형성은 크게 감소하였지만, S. gullinarum은 소낭 형성 감소에 영향을 주지 않았다. 두 세균 모두는 prophenoloxidase (proPO)의 활성화를 억제시켰다. 그러나 X. nematophilus.가 S. gallinarum보다 높게 PO 활성화를 억제시켰다. 라이소자임의 활성유도는 S. gallinarum접종 후 4시간에서 관찰되었으나, X. nematophilus를 접종하였을 때는 라이소자임의 활성이 전혀 관찰되지 않았다 이러한 결과들은 누에에서 X. nematophilus가 S. gallinarum보다 더 큰 면역저하를 유발하여 병원성을 높였다는 것을 보여준다. 따라서 본 연구는 곤충병원세균에 의해서 유발된 면역저하는 곤충병원세균의 병원성과 연관되어 있다는 것을 제시한다.다.