To explore cattail pollen powder as a functional food ingredient, we analyzed the general components of pollen powder, tested changes in the physical properties of dough containing the powder, and investigated the process ability of powder-containing dough in bread making by adding 3%, 6%, or 9% by weight of pollen powder to wheat flour. Cattail pollen powder consisted of (all w/w) 12.7-13.2% water, 15.7-17.8% crude protein, 1.3% crude fat, 7.5-7.7% free sugar, 14.7-18.6% crude fiber, 3.4-4.9% pollen, and 49.7-55.9% soluble nitrogen-free extract (NFE). Analysis of the physical properties of dough mixed with pollen powder showed that as more pollen powder was added, the absorption rate increased, but dough stability decreased. With increasing levels of cattail pollen powder, the falling number decreased, and amylase activity increased. Fermentability was highest in dough made with 3% by weight of pollen powder, and the bread product made from such dough had the greatest volume. As more cattail pollen powder was added, the moisture activity in dough tended to decrease to a greater extent than seen in control dough, and this tendency increased with time. We found that longer storage periods were associated with greater hardness and springiness, which indicated degradation in product quality. Therefore, it is suggested that bread products containing cattail pollen powder should be consumed within 3 days of preparation. In a taste survey, bread baked with 3% (w/w) cattail pollen powder scored highest in all questionnaire items.
Next-generation sequencing (NGS) is a high-throughput technique for sequencing large numbers of DNA fragments that are prepared from a genome. This sequencing technique has been used to elucidate whole genome sequences of living organisms and to analyze complementary DNA (cDNA) or chromatin immunoprecipitated DNA (ChIPed DNA) at the genome level. After NGS, the use of proper tools is important for processing and analyzing data with reasonable parameters. However, handling large-scale sequencing data and programing for data analysis can be difficult. The Galaxy platform, a public web service system, provides many different tools for NGS data analysis, and it allows researchers to analyze their data on a web browser with no deep knowledge about bioinformatics and/or programing. In this study, we explain the procedure for preparing chromatin immunoprecipitation-sequencing (ChIP-seq) libraries and steps for analyzing ChIP-seq data using the Galaxy platform. The data analysis steps include the NGS data upload to Galaxy, quality check of the NGS data, premapping processes, read mapping, the post-mapping process, peak-calling and visualization by window view, heatmaps, average profile, and correlation analysis. Analysis of our histone H3K4me1 ChIP-seq data in K562 cells shows that it correlates with public data. Thus, NGS data analysis using the Galaxy platform can provide an easy approach to bioinformatics.
Objectives: This study examined the practices of a community meal program for older adults in rural areas during the agricultural off-season. Methods: A survey was conducted from December 12 to December 22, 2016. Self-administered questionnaires were distributed to 150 cooking volunteers, who had participated in the community meal program in 50 villages. A total of 114 responses were returned from 44 villages and used for data analysis. In addition, in-depth interviews were conducted with the volunteers of eight villages. Results: Most of the cooking volunteers were 50 years old or older and they participated in serving older adults meals for good will. The cooking volunteers perceived that the older adults in their community did not eat various foods, had difficulties in grocery shopping, and frequently consumed salty foods. During the agricultural off-season, 40.9% of villages served the older adults meals 6-7 days a week and 95.5% provided meals for lunch. An average of 21 to 40 older adults were served meals in each village. The cooking volunteers reported that the food preparation and meal service times were sufficient, recipes provided were useful, and menus met the preference of the older adults. At the end of the program, they felt proud of serving meals for older adults in the community. An increased awareness of healthy eating, interest in health, and consumption of nutritious meals, a decrease in loneliness among older adults, and the promotion of fellowship in the community were rated highly. The cooking volunteers expected additional support for cooking personnel and insisted that the program should be provided for the entire agricultural off-seasons. Conclusions: The community meal program during the agricultural off-season for the elderly in rural areas was effective in improving the dietary life of older adults, relieving their feelings of isolation, and promoting fellowship of the community. The volunteers felt workload due to a shortage of volunteers but answered that they were rewarded by helping older adults in their community.
In the fourth industrial revolution and the era of convergence and integration, on the situation that the internal colleges are needed active change included in the improvement of educational quality, I tested it on the purpose of empirical analysis with SPSS v.18 how colleges' efforts on the first periodic Organization Evaluation And Accreditation System(OEAAS) affects on the Perceived Management Performances on the perspective of BSC. As the test result, the Degree of Awareness of Colleges' Efforts on the OEAAS affects on just Colleges' Learning and on Growth. The Degree Propriety of Preparation of the OEAAS affects on Customer Performance, on Internal Process Performance, and, on Finance Performance. And the Degree of Satisfaction of Internal Assessment affects on all of BSC 4 performances. The results of this research could be used on making the management idea of colleges' performance on the OEAAS. In the future, it would be needed advanced researches which are able to make relatedness to the expanse of management performance with the OEAAS.
Non-fluorinated water repellent coating solutions were obtained using methyltrimethoxysilane (MTMS) and trimethylethoxysilane (TMES) as precursors. The solutions were spin-coated on a cold-rolled steel sheet and cured thermally to prepare water repellent coating films. During this process, the effect of molar ratio of TMES/MTMS was studied for the hydrophobic properties of the coating films. Hydrophobic properties of coating films were characterized using contact angle measurement, surface morphology analysis and infrared spectroscopy. When the molar ratio of TMES/MTMS was varied from 0 to 30, the contact angle of the un-coated cold-rolled steel sheet was $30^{\circ}$, whereas when the molar ratio of TMES/MTMS was 1, the contact angle increased to $104^{\circ}$ and water repellency was significantly improved. In the case of TMES/MTMS molar ratios of 10, 15, 25 and 30, the contact angles of coating films showed $109^{\circ}$, $114^{\circ}$, $117^{\circ}$ and $144^{\circ}$, respectively. At this time, the hydrophobicity of the coating films was improved by the increase of the surface roughness and the content of the methyl component at the coating surface. In particular, when the molar ratio of TMES/MTMS was 30, the overall surface roughness was greatly increased due to the presence of surface particles as well as the water repellency due to methyl groups of TMES, resulting in super hydrophobicity of $144^{\circ}$.
Porous nanofibers comprising hollow ${\alpha}-Fe_2O_3$ nanospheres were prepared by applying both template method and Kirkendall diffusion effect to electrospinning process. During heat-treatment processes, the solid Fe nano-metals formed by initial heat-treatment in the carbon matrix were converted into the hollow structured ${\alpha}-Fe_2O_3$ nanospheres. In particular, PS nanobeads added in the spinning solution were decomposed and formed numerous channels in the composite, which served as a good pathway for Kirkendall diffusion gas. The resulting porous nanofibers comprising hollow ${\alpha}-Fe_2O_3$ nanospheres were applied as an anode material for lithium-ion batteries. The discharge capacities of the nanofibers for the 30th cycle at a high current density of $1.0A\;g^{-1}$ was $776mA\;h\;g^{-1}$. The good lithium ion storage property was attributed to the synergetic effects of the hollow ${\alpha}-Fe_2O_3$ nanospheres and the interstitial nanovoids between the nanospheres. The synthetic method proposed in this study could be applied to the preparation of porous nanofibers comprising hollow nanospheres with various composition for various applications, including energy storage.
Membrane proteins are involved in many common diseases, including heart disease and cancer. In various disease states, such as cancer, abnormal signaling pathways that are related to the membrane proteins cause the cells to divide out of control and the expression of membrane proteins can be altered. Membrane proteins have the hydrophobic environment of a lipid bilayer, which makes an analysis of the membrane proteins notoriously difficult. Therefore, this study evaluated the efficacy of two different methods for optimal membrane protein extraction. High-speed centrifuge and reagent-based method with a -/+ filter aided sample preparation (FASP) were compared. As a result, the high-speed centrifuge method is quite effective in analyzing the mitochondrial inner membranes, while the reagent-based method is useful for endoplasmic reticulum membrane analysis. In addition, the function of the membrane proteins extracted from the two methods were analyzed using GeneGo software. GO processes showed that the endoplasmic reticulum-related responses had higher significance in the reagent-based method. An analysis of the process networks showed that one cluster in the high-speed centrifuge method and four clusters in the reagent-based method were visualized. In conclusion, the two methods are useful for the analysis of different subcellular membrane proteins, and are expected to assist in selecting the membrane protein extraction method by considering the target subcellular membrane proteins for study.
This study analyzes the technology billionaires with the combination of technology commercialization studies and billionaire research. However, we will not discuss individual billionaires, but discuss the general features of technology billionaires. This study assumes that the answers to the technology commercialization studies are in the technology billionaire researches. In other words, unlike the technology commercialization studies so far, it can be said that it is a retrospective approach of technology commercialization to see the process and the beginning from the result. The conclusion of this study is as follows. First, technology billionaires are in the middle rank among industries, but their wealth is the best. Second, in the technology sector, four 20s are self-made billionaires. It is a matter of securing technological opportunities, not a long training and preparation. Third, the determinants of technology billionaires are population size and venture capital investment. This means technological efforts and sufficient market conditions are the basis for the development of technology billionaires. Fourth, only high income is not the determinant of technology billionaires. There are many small countries that are very rich but can not utilize technology. Technology billionaires can appear in countries with a minimum of US $ 20,000, unless the country has a huge population such as China and India. Sixth, technology billionaires in the diversified business, classified as Chaebol in low-income countries become scarce in the countries over the US $ 40,000. Billionaires will increase rapidly from the mid-2000s. However, this is not explained by the income increase effect. The world's income increase has been slowing since the 1980s. The market economy effect of the socialist countries is not the reason, because it is limited to some socialist countries. Ultimately, the main reason is the new technology opportunity called the IT paradigm.
Journal of the Korean Institute of Traditional Landscape Architecture
/
v.36
no.4
/
pp.105-112
/
2018
This study aims to apply 3D scanning technology to the field of landscape planning design. Through this, 3D scans were conducted on Soswaewon Garden and Seongrakwon Gardens to find directions for traditional landscape planning and designs. The results as follows. First, the actual measurement of the traditional garden through a 3D scan confirmed that a precise three-dimensional modeling of ${\pm}3-5mm$ error was constructed through the merging of coordinate values based on point data acquired at each observation point and postprocessing. Second, as a result of the 3D survey, the Soswaewon Garden obtained survey data on Jewoldang House, Gwangpunggak Pavilion, the surrounding wall, stone axis, and Aeyangdan wall, while the Seongnakwon Garden obtained survey data on the topography, rocks and waterways around the Yeongbyeokji pond area. The above data have the advantage of being able to monitor the changing appearance of the garden. Third, spatial information developed through 3D scans could be developed with a three-dimensional drawing preparation and inspection tool that included precise real-world data, and this process ensured the economic feasibility of time and manpower in the actual survey and investigation of landscaping space. In addition, modelling with a three-dimensional 1:1 scale is expected to be highly efficient in that reliable spatial data can be maintained and reprocessed to a specific size depending on the size of the design. In addition, from a long-term perspective, the deployment of 3D scan data is easy to predict and simulate changes in traditional landscaping space over time.
Titania has become the most applicable material for photocatalytic application. Nevertheless, titania has the weak point in its wide band gap energy that is mainly activated by UV irradiation. There have been vast research challenges in order to make the wide band gap energy of titania narrow that could be activated in the presence of visible light. Various modifications of titania surface were popular because titania needs to change its surface to respond in visible light. Among the methodological approaches, N-doping to titania can be the alternative candidate because it is facile process and eco-friendly. The activated electron from valence band in N-doped $TiO_2$ migrates to conduction band in the presence of visible light irradiation, which shows photocatalytic activity as well. In this study, focused on the evaluation of nitrogen state after N-doping through brief review. Arguments are still existed in nitrogen states and their different effects on photocatalytic activity. In particular, two nitrogen states are generally reported; substitutional and interstitial states. The research articles regarding N-doped $TiO_2$ are continuously appearing because the potential application of water split in visible light is still fascinate. The future of N-doped $TiO_2$ is also presented by referrals based on various literature.
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