• 한국수자원학회:학술대회논문집
• /
• 한국수자원학회 2006년도 학술발표회 논문집
• /
• pp.12-17
• /
• 2006

Natural rivers have water purification functions called Gravel Contact Oxidation Process, which decontaminate river water by biological absorption, oxidation and degradation on riverbed gravels. This function has been developed and applied to many small/medium-sized urban rivers in Japan as one of the direct river water purification methods. However the method hasn't been verified in the tropics, which have different climate conditions and river characteristics. A preliminary experiment carried out at a polluted urban tributary in the outskirts of Kuala Lumpur, Malaysia where an increasing attention has been paid to river environment, showed a good applicability to the tropical conditions as a technically practical water purification measure with some maintenance cares for sludge management.

• 통합자연과학논문집
• /
• 제6권4호
• /
• pp.193-196
• /
• 2013

Haemoglobin is an interesting physiologically significant protein composed of specific functional prosthetic haem and globin moieties. In recent decades, there has been substantial interest in attempting to understand the structural basis and functional diversity of avian haemoglobins (Hbs). Towards this end, purification, crystallization, preliminary X-ray diffraction and molecular-replacement studies have been carried out on Amaurornis phoenicurus Hb. Crystals were grown by the hanging drop vapor-diffusion method using PEG 2000 and NaCl as precipitants. The crystals belonged to the primitive monoclinic system $P2_1$, with unit-cell parameters $a=65.33{\AA}$, $b=93.14{\AA}$, $c=98.54{\AA}$, ${\beta}=100.48^{\circ}$; a complete data set was collected to a resolution of $2.6{\AA}$. The Matthews coefficient of $2.30{\AA}^3Da^{-1}$ for the crystal indicated the presence of two ${\alpha}_2{\beta}_2$ tetramers in the asymmetric unit.

• 한국식품과학회지
• /
• 제48권6호
• /
• pp.542-547
• /
• 2016

The thermal properties of ramie leaf ${\beta}$-amylase (RBA) were examined to develop a novel process for enzyme purification. The thermostability of RBA extract prepared from ramie leaf powder was examined at various temperatures. RBA activity decreased slightly, whereas other carbohydrate-active enzymes, such as $\small{D}$-enzyme, were rapidly inactivated during 30 min incubation at $60^{\circ}C$. When the heat-treated extract was incubated with various substrates, maltose was produced exclusively as the major product, whereas the untreated crude extract produced maltose and other maltooligosaccharides. In sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis, fewer protein bands were observed for the heat-treated extract than the untreated extract, indicating that the thermostable RBA was partially purified and other thermolabile enzymes were eliminated. Thus, the treatment of the RBA extract at $60^{\circ}C$ for 30 min resulted in 5.4-fold purification with a recovery yield of 90%.

• 한국축산식품학회지
• /
• 제38권1호
• /
• pp.52-63
• /
• 2018

Cheese consumption has been gradually increased in China. However, both the manufacturing process of cheese and the utilization of its main by-product were not well developed. Based on the sensory evaluation, Box-Behnken Design (BBD) was performed in the present study to optimize the cheese processing, which was proved more suitable for Chinese. The optimal parameters were: rennet 0.052 g/L, start culture 0.025 g/L and $CaCl_2$ 0.1 g/L. The composition analysis of fresh bovine milk and whey showed that whey contained most of the soluble nutrients of milk, which indicated that whey was a potential resource of cyclic adenosine-3', 5'-monophosphate (cAMP). Thus, the cAMP was isolated from whey, the results of high-performance liquid chromatography (HPLC) analysis showed that the macroporous adsorption resins (MAR) D290 could increase the concentration of cAMP from $0.058{\mu}mol/mL$ to $0.095{\mu}mol/mL$. We firstly purified the cAMP from the whey, which could become a new source of cAMP.

• 한국지반환경공학회 논문집
• /
• 제17권10호
• /
• pp.63-71
• /
• 2016

국내 폐석면 오염지역의 복원공사는 대부분이 토양개량 공법인 복토와 환토 공법을 일률적으로 적용하고 있으나 토양개량 방법만으로는 석면오염을 근원적으로 해결할 수가 없어 새로운 대체기술에 대한 연구가 수행되어오고 있다. 본 연구에서는 풍력장치를 이용한 실내시험 결과를 종합하여 실제 현장에 적용할 수 있는 풍력정화장치를 설계 제작하였으며, 규회석을 이용한 예비시험 및 실제 석면오염토를 이용하여 농경지토양에 대한 정화효율에 대한 연구를 수행하였다. 석면오염토에 대한 현장시험결과 토출직후 석면함유량은 모두 0.25% 미만으로 정화기준을 만족하였으며, 청토회수율 90% 이상을 확보한 것으로 분석되어 향후 석면오염토양 정화분야의 적용성이 뛰어난 것으로 판단된다.

• Asian Pacific Journal of Cancer Prevention
• /
• 제15권20호
• /
• pp.8685-8688
• /
• 2014

The objective of the present study was to investigate cloning, expression, and functions of the recombinant protein, Siva1. Siva1 gene was synthesized by RT-PCR from HCT116 cells. Plasmids were cleaved with the restriction endonuclease, BamH1/Sal1 and products were connected to pQE30, which underwent cleavage by BamH1/Sal1. The recombinant plasmid, pQE30-Siva1, was identified after digestion with restriction endonucleases followed by transformation into E. coli M15. Expression of Siva1 was induced by IPTG and identified by SDS-PAGE following purification with affinity chromatography. The results showed that size of Siva1 was 12 kDa, consistent with the molecular weight of the His-Siva1 fusion protein. Functional test demonstrated that Siva1 significantly inhibited the invasion and migration of HCT116 cells. It may thus find clinical application for control of cancers.

• Archives of Plastic Surgery
• /
• 제33권1호
• /
• pp.21-30
• /
• 2006

Alginate, a polymer of guluronic and mannuronic acid, is used as a scaffolding material in biomedical applications. The research was to produce highly-purified alginate from seaweeds and to evaluate the efficacy of alginate as dermal substrate. Our alginate purification method showed a production rate as high as 25%. The purified alginate contained little polyphenol contents and endotoxin, proteins. For study of wound healing, full thickness skin defects were made on the dorsal area of the animal models. And then alginate, fibroblast-growth-factor mixed alginate, alginate-collagen complex, vaseline gauze as control were applied on the wound, respectively, and were evaluated grossly and histopathologically. For biocompatibility test, alginate and alginate-collagen complex discs were implanted on the back of Sprague-Dawly rats. Four weeks after implantation, the animals were examined immunologically against alginate and collagen. Alginate and FGF-mixed alginate, alginate-collagen complex group showed statistically higher percentage of wound contraction and wound healing than control group(p<0.05). Alginate-collagen complex group and FGF-mixed alginate group showed statistically higher percentage of wound healing than alginate group. The experiment of biocompatibility and immunologic reaction against impanted alginate or collagen needs more investigation. Highly-purified alginate from seaweeds by our purification method, showed the effect of wound healing, and addition of FGF or collagen increases the alginate's wound healing effect. It shows the possibility of alginate as a dermal substrate.

• Biodesign
• /
• 제6권4호
• /
• pp.96-99
• /
• 2018

The gram-positive bacterium Staphylococcus aureus is a common cause of abscesses, sinusitis and food poisoning. The emergence of antibiotic-resistant strains has caused significant clinical issues worldwide. The HslU-HslV complex was first identified as a prokaryotic homolog of eukaryotic proteasomes. HslU is an unfoldase that mediates the unfolding of the substrate proteins, and it works with the protease HslV in the complex. To date, the protein complex has been mostly studied in gram-negative bacteria. In this study, we report the purification and crystallization of the full-length HslU from S. aureus. The crystal diffracted X-rays to a $3.5{\AA}$ resolution, revealing that the crystals belong to space group $P2_1$, with unit cell parameters of a = 166.5, b = 189.6, $c=226.6{\AA}$, and ${\beta}=108.1^{\circ}$. We solved the phage problem by molecular replacement using the structure of HslU from Haemophilus influenzae as a search model. The cell content analysis with this molecular replacement solution revealed that 24 molecules are contained in the asymmetric unit. This structure provides insight into the structural and mechanistic difference of the HslUV complex of gram-positive bacteria.

• 한국식품과학회지
• /
• 제4권4호
• /
• pp.252-258
• /
• 1972

경유(輕油)를 기질(基質)로 한 석유탄화수소발효액(石油炭化水素醱酵液)으로부터 효과적(效果的)으로 균체(菌體)를 회수(回收), 정제(精製)하기 위한 몇가지 지견(知見)과 예비동물사육시험(飼備動物飼育試驗)결과를 요약(要約)하면 다음과 같다. 1) 발효배양액(醱酵培養液)으로부터 균체(菌體) cream의 정치분리(精置分離)는 30분만에 종료(終了)되며 이때 cream의 균체농도(菌體濃渡)는 5.6%였다. 2) pH 9 이상의 강알칼리성에서 균체(菌體) emulsion은 불안정(不安定)하였으며 열처리(熱處理), 동결(凍結)에 의하여 emulsion으로부터 $60{\sim}80%$의 균체(菌體)를 분리(分離)할 수 있었다. 3) Emulsion 파괴(破壞)를 위한 식용계면활성제(食用界面活性劑)로는 트리오가 가장 우수하였으며 그 적합한 농도(濃渡)는 0.3%였다. 이 외에 Aldo, Monogly 5-7 및 G-4도 유효(有效)하였다. 4) NaCl, KCl 등의 할로겐화알칼리금속염이 균체(菌體) emulsion 파괴(破壞)에우수하였으며 트리오와 같은 계면활성제(界面活性劑)와 혼합(混合)하여 사용하므로서 90% 이상의 균체(菌體)를 분리(分離)할 수 있었다. 5) 건조반제품(乾燥半製品)을 hexane-ethyl alcohol 및 iso-propyl alcohol의 공비혼합물(共沸混合物)로 유출(抽出)하는 것이 가장 기대(期待)되는 균체(菌體)의 정제방법(精製方法)이며 추출온도(抽出溫度)는 $58^{\circ}C$, 추출시간(抽出時間)은 30분(分), 균체(菌體) 대(對) 용매비율(溶媒比率)은 1 : 3이 적합하였다. 6) 균체단백질(菌體蛋白質)의 사양실험결과 어백과 5% 대체(代替)한 수준(水準)에시는 대조구(對照區)보다 3.3%의 더 좋은 증체(增體)를 보였으나 8% 대체구(代替區)는 다른 처리구에 비하여 간(肝)중량이 더 무거운 것이 인정되었다.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2002년도 생물공학의 동향 (X)
• /
• pp.500-503
• /
• 2002

In order to improve the physical properties of food such as texture and food self-life. Transglutaminase(mTG) from Streptoverticillum morbarense was prepared. In the preliminary experiments, presence of proteases in the crude enzyme did not improve the texture of dough, which mean the inteference of mTG reaction by the proteases. Among the cation exchange resins tested for the removal of proteases, Monoplus S 100(Bayer, Germany) was the most efficient resin with 20 fold increase in the mTG/protease activity ratio. By further purification steps with a quaternary ammonia salt resin and a gel permeation chromatography, proteases were effectively removed from the preparation. Therefore, the improvement of flour texture was shown by adding the protease-free mTG.