Archives of Plastic Surgery

Korean Society of Plastic and Reconstructive Surgeons (대한성형외과학회)
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An Developmental Study of Artificial Skin Using the Alginate Dermal Substrate: Preliminary Report

알지네이트 진피지지체 인공피부 개발: 예비보고

  • Park, Dae Hwan (Department of Plastic and Reconstructive Surgery, Daegu Catholic University) ;
  • Shin, Jeong Im (School of Medicine & Tissue Engineering Center, Daegu Catholic University)
  • 박대환 (대구가톨릭대학교 의과대학 성형외과학교실) ;
  • 신정임 (대구가톨릭대학교 의과대학 조직공학센터)
  • Received : 2005.07.29
  • Published : 2006.01.10
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Abstract

Alginate, a polymer of guluronic and mannuronic acid, is used as a scaffolding material in biomedical applications. The research was to produce highly-purified alginate from seaweeds and to evaluate the efficacy of alginate as dermal substrate. Our alginate purification method showed a production rate as high as 25%. The purified alginate contained little polyphenol contents and endotoxin, proteins. For study of wound healing, full thickness skin defects were made on the dorsal area of the animal models. And then alginate, fibroblast-growth-factor mixed alginate, alginate-collagen complex, vaseline gauze as control were applied on the wound, respectively, and were evaluated grossly and histopathologically. For biocompatibility test, alginate and alginate-collagen complex discs were implanted on the back of Sprague-Dawly rats. Four weeks after implantation, the animals were examined immunologically against alginate and collagen. Alginate and FGF-mixed alginate, alginate-collagen complex group showed statistically higher percentage of wound contraction and wound healing than control group(p<0.05). Alginate-collagen complex group and FGF-mixed alginate group showed statistically higher percentage of wound healing than alginate group. The experiment of biocompatibility and immunologic reaction against impanted alginate or collagen needs more investigation. Highly-purified alginate from seaweeds by our purification method, showed the effect of wound healing, and addition of FGF or collagen increases the alginate's wound healing effect. It shows the possibility of alginate as a dermal substrate.

Keywords

Acknowledgement

Supported by : 한국학술진흥재단

References

  1. Jeong JH, Kim KH, Kim HH, Moo YM, Lee DJ, Choi JH, Kim SY: Injectable cartilage tissue engineering techniques utilizing fibrin glue, liquid alginate and pluronic F-127. J Korean Soc Plast Reconstr Surg 30: 465, 2003
  2. Srnidsrod 0, Skjak-Bnek G: Alginate as immobilization matrix for cells. Trends Biotechnol 8: 71, 1990 https://doi.org/10.1016/0167-7799(90)90139-O
  3. Grandolfo M, D'Andrea P, Paoletti S, Martina M, Silvestrini G, Bonucci E, Vittur F: Culture and differentiation of chondrocytes entrapped in alginate gels. Calcif Tissue Int 52: 42, 1993 https://doi.org/10.1007/BF00675625
  4. Paige KT, Cima LG, Yaremchuk MJ, Vavanti JP, Vacanti CA: Injectable cartilage. Plast Reconstr Surg 96: 1390, 1995 https://doi.org/10.1097/00006534-199511000-00024
  5. Yeo, G, Lee CH, Rhie JW, Wee 55: The role of alginate sponge on growth and differentiation of preadipocytes. J Korean Soc Plast Reconstr Surg 28: 210, 2001
  6. van Susante JL, Buma P, van Osch GJ, Versleyen D, van der Kraan PM, Van der Berg WB, Homminga GN: Culture of chondrocytes in alginate and collagen carrier gels. Acta Orthop Scand 66: 549, 1995 https://doi.org/10.3109/17453679509002314
  7. Thie M, Schlumberger W, Rauterberg J, Robenek H:Mechanical confinement inhibits collagen synthesis in gelcultured fibroblasts. Eur J Cell BioI 48: 294, 1989
  8. Hilliou F, Pairault J, Dominice J, Redziniak G: Growth and diffentiation of 3T3-F442A preadipocytes in threedimensional gel of native collagen. Exp Cell Res 177: 372, 1988 https://doi.org/10.1016/0014-4827(88)90470-3