• International Journal of Oral Biology
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• 제38권3호
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• pp.111-119
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• 2013

Objective. To investigate the effects of the hypoxia inducible factor-1 (HIF-1) activation-mimicking agent cobalt chloride ($CoCl_2$) on the osteogenic differentiation of human mesenchymal stem cells (hMSCs) and elucidate the underlying molecular mechanisms. Study design. The dose and exposure periods for $CoCl_2$ in hMSCs were optimized by cell viability assays. After confirmation of $CoCl_2$-induced HIF-$1{\alpha}$ and vascular endothelial growth factor expression in these cells by RT-PCR, the effects of temporary preconditioning with $CoCl_2$ on hMSC osteogenic differentiation were evaluated by RT-PCR analysis of osteogenic gene expression, an alkaline phosphatase (ALP) activity assay and by alizarin red S staining. Results. Variable $CoCl_2$ dosages (up to $500{\mu}M$) and exposure times (up to 7 days) on hMSC had little effect on hMSC survival. After $CoCl_2$ treatment of hMSCs at $100{\mu}M$ for 24 or 48 hours, followed by culture in osteogenic differentiating media, several osteogenic markers such as Runx-2, osteocalcin and osteopontin, bone sialoprotein mRNA expression level were found to be up-regulated. Moreover, ALP activity was increased in these treated cells in which an accelerated osteogenic capacity was also verified by alizarin red S staining. Conclusions. The osteogenic differentiation potential of hMSCs could be preserved and even enhanced by $CoCl_2$ treatment.

• 정보처리학회논문지A
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• 제10A권2호
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• pp.137-140
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• 2003

최근에 CG 반복법을 이용하여 레이레이 계수를 최소화함으로써 대칭행렬의 내부고유치를 구하는 방법이 개발되었다 그리고 이 방법은 병렬계산에 매우 적합하다. 적절한 준비행렬의 선택은 수렴속도를 향상시킨다. 우리는 본 연구에서 이를 위한 병렬준비행렬들을 비교한다. 고려된 준비행렬들은 Point-SSOR, 다중색채하의 ILU(0)와 Block SSOR이다. 우리는 128개의 노드를 가진 CRAY-T3E에서 구현하였다. 프로세서간의 통신은 MPI 리이브러리를 사용하였다. 최고 512$\times$512 행렬까지 시험하였는데 이 행렬들은 타원형 편미분방정식의 근사화에서 얻어졌다. 그 결과 다중색채 Block SSOR이 가장 성능이 우수한 것으로 판명되었다.

• 약학회지
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• 제41권6호
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• pp.829-836
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• 1997

Studies on the effect of quinones on cardiac function has been conducted with normal hearts. But not with injured hearts, I.e. ischemia/reperfusion-injured heart. Quinone compounds are known to produce oxygen free radicals during metabolism, and for this reason, quinones are implicated in the aggravation of ischemia/reperfusion injury or cardioprotection, as in the case of ischemic preconditioning depending on the experimental conditions. The present study was carried out to examine the effect of 2-chloro-3-(4-cyanophenylamino)-1.4-naphthoquinone (NQ-Y15) on cardiac function of ischemic/reperfused and normal rat hearts. In isolated perfused hearts, various functional parameters such as left ventricular developed pressure (LVDP), left ventricular end-diastolic pressure (EDP) and maximum positive and negative dP/dt ($[\pm}dP/dt_{max}$), time to contracture, heart rate (HR) and coronary flow rate (CFR) were measured before and 30 min after dosing and following 25 min ischemia/30min reperfusion. NQ-Y15 increased LVDP, +dP/$d_{max}$and -dP/$dt_{min}$ by 18%. 30%, and 40%, respectively. There were no significant changes in other haemodynamic parameters. After ischemia/reperfusion injury, pretreatment with NQ-Y15 induced a significant decrease in LVDP and $[\pm}dP/dt_{max}$, but an increase in EDP. LDH-release was not significantly increased. These results suggested that NQ-Y15 may augment the ventricular contractility but it makes hearts more vulnerable to ischemia/reperfusion injury.

• 한국추진공학회지
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• 제17권6호
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• pp.48-58
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• 2013

4개 화학종의 케로신 대체 모델과 다양한 상태 방정식을 이용하여 초임계 압력 조건의 스월 인젝터에서의 열역학 상태량 및 유동 특성을 연구하였다. 상태방정식에 따른 열역학 물성치의 정량적인 비교와 함께 large eddy simulation 및 예조건화 기법을 적용하여 스월 인젝터에서의 유동 특성 해석이 수행되었다. 초임계 스월 인젝터 내에서 열역학 물성치를 비교 조사하였고 상태량 예측 정확도가 인젝터에서의 유동 특성에 상당한 영향을 미치는 것이 관찰되었다. Redlich-Kwong-Peng-Robinson 상태 방정식이 넒은 온도 영역에서 가장 적절한 결과를 제시하였다.

• 한국환경보건학회지
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• 제19권3호
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• pp.1-16
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• 1993

This paper describes an investigation to determine whether the activated sludge (AS) process could be used for the treatment of wastewater at the Union Carbide Coporation (UCC) plant in Seadrift, Texas. This plant presently utilizes a waste stabilization pond (WSP) system for treatment of the wastewater. The treatment system consists of an in-plant primary WSP and two off-plant WSPs (secondary and tertiary WSPs), run in series. The total hydraulic detention time of the WSP system is approximately 150 days. Several laboratory-based treatability studies have been conducted to evaluate the performace of the WSP system and the degradability of specific chemical compounds. From an additional study, it was determined that the WSP system was stressed and occasionally operating near the limit of its treatment capacity. The existing primary WSP plays an important role in the overall treatmemt system, because it not only functions as a pH and organic-strength equalization basin, but also serves as a "preconditioning" basin by fermenting high strength organic wastes to volatile organic acids for subsequent degradation in the escondary WSP. However, in view of pending RCRA legislatin conerning the "proposed organic toxicity characteristics limits" (40 CFR Part261: Federal Register, July, 1988), it is possible that the primary WSP will have to be abandoned in favor of alternative treatment options. Therefore the main purpose of this study was to perform activated sludge treatability evaluations for the development of an alternative to the existing primary WSP treatment ststem. In addition, another purpose was to determine the degradability of bis(2-chloroethyl)ether (Chlorex or CX) and benzene(BZ) in the activated sludge process. The presence of these two chemicals in the wastewater of the plant prompted the question of whatedether they could be degraded in an activated sludge system.

• Parasites, Hosts and Diseases
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• 제58권4호
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• pp.461-466
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• 2020

Toxoplasma gondii is an obligate intracellular protozoan parasite that can invade various organs in the host body, including the central nervous system. Chronic intracranial T. gondii is known to be associated with neuroprotection against neurodegenerative diseases through interaction with host brain cells in various ways. The present study investigated the neuroprotective effects of chronic T. gondii infection in mice with cerebral ischemia experimentally produced by middle cerebral artery occlusion (MCAO) surgery. The neurobehavioral effects of cerebral ischemia were assessed by measurement of Garcia score and Rotarod behavior tests. The volume of brain ischemia was measured by triphenyltetrazolium chloride staining. The expression levels of related genes and proteins were determined. After cerebral ischemia, corrected infarction volume was significantly reduced in T. gondii infected mice, and their neurobehavioral function was significantly better than that of the uninfection control group. Chronic T. gondii infection induced the expression of hypoxia-inducible factor 1-alpha (HIF-1α) in the brain before MCAO. T. gondii infection also increased the expression of vascular endothelial growth factor after the cerebral ischemia. It is suggested that chronic intracerebral infection of T. gondii may be a potential preconditioning strategy to reduce neural deficits associated with cerebral ischemia and induce brain ischemic tolerance through the regulation of HIF-1α expression.

• 한국환경복원기술학회지
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• 제24권2호
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• pp.115-126
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• 2021

Strategic Environmental Impact Assessment (SEA) is a decision-making process taking into account the environmental impact, economic and social impact of policies, plans, and programs at the higher stage prior to the project plan for promoting sustainable development. In this study, we analyzed the process and criteria for selecting appropriate alternatives when establishing development plan in SEA. First, the criteria for estimating changes in ecosystem services following the implementation of development project of industrial complex were presented. Second, alternative evaluations were conducted through an analysis of ecosystem service scenarios to explore suitable alternatives in Anseong. As a result, the environmental quality of selected area as the existing project site deteriorated according to the implementation of the project, and the dimensional reduction technique confirmed that the change in ecosystem service factors in project area was the optimal location. In addition, the results of the scenario assessment to explore suitable alternatives in Anseong City showed that the existing site had large capacity in terms of water quality control services (scenario 1), scenario 2 in terms of preconditioning services, and scenario 3 in terms of water supply services. The guidance of Ecosystem service assessment is expected to be available in decision-making of large-scale strategies (e.g., SEA) and projects by presenting more quantitative criteria for determining the adequacy and location feasibility of development plans and policy plans. This is expected to require various support, including legislation and revision of related laws, believed to be supported by advanced research.

• 대한수의학회지
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• 제58권4호
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• pp.211-217
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• 2018

Mesenchymal stem cells (MSCs) are useful candidates for tissue engineering and cell therapy. Physiological cell environment not only connects cells to each other, but also connects cells to the extracellular matrix that provide mechanical support, thus exposing the entire cell surface and activating signaling pathways. Hydrogel is a polymeric material that swells in water and maintains a distinct 3-dimensional (3D) network structure by cross linking. In this study, we investigated the optimized cellular function for canine adipose tissue-derived MSCs (cAD-MSCs) using hydrogel. We observed that the expression levels of Ki67 and proliferating cell nuclear antigen, which are involved in cell proliferation and stemness, were increased in transwell-hydrogel (3D-TN) compared to the transwell-normal (TN). Also, transforming growth factor-${\beta}1$ and SOX9, which are typical bone morphogenesis-inducing factors, were increased in 3D-TN compared to the TN. Collagen type II alpha 1, which is a chondrocyte-specific marker, was increased in 3D-TN compared to the TN. Osteocalcin, which is a osteocyte-specific marker, was increased in 3D-TN compared to the TN. Collectively, preconditioning cAD-MSCs via 3D culture systems can enhance inherent secretory properties that may improve the potency and efficacy of MSCs-based therapies for bone regeneration process.

• 아태비즈니스연구
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• 제12권1호
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• pp.41-56
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• 2021

Purpose - This empirical study examined the influence of post-communist countries sociol-political factor of freedom of expression preconditioning, and trust in agency, mediating performance expectancy of social media users representing the citizens' behavioural intention to utilize social media in a post-communist country, the case of Mongolia. Design/methodology/approach - This research collected 403 valid survey data from citizens those who use social media in Mongolia. The study used Partial Least Squire (PLS) analysis with the research conceptual model founded on the UTAUT model. Findings - The study shown that citizens in post-communist, they strongly willing freedom of expression, which driving as a positive precondition factor, and this has an indirect positive influence, and trust in agency mediates to enhance performance expectancy. Social influence, and effort expectancy factors have direct positive influence on the intention to use of social media systems in the public sector domain of Mongolia. Research implications or Originality - This research proposed a new model to test citizens' intention to use social media as a communication tool to engage with public organizations in the pre-adoption stage of post-communist countries. Theoretically, this research builds up to the unique theoretical contribution with social media by examining a new social media-based third-party intercommunication medium, incorporating intent to utilize for citizens with government in post-communist countries. Practically, this article lays out the directions to aid social media usage for government sector with concerning citizens intentions in the post-communist situation.

• 아태비즈니스연구
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• 제11권2호
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• pp.61-79
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• 2020

Purpose - This article empirically investigated the effects of the socio-political factor of censorship preconditioning, and organizational support, mediating performance expectancy of public sector officials' behavioural intention to utilise social media in a post-communist country, Mongolia. Design/methodology/approach - This study collected 212 survey data from public sector organisations in Mongolia. Using the Partial Least Squire (PLS) method, this study analyzed the proposal model grounded on the UTAUT model. Findings - There are still communist footprints in the form of censorship, which remained as a negative precondition factor, and this has an indirect negative influence, and organisational support mediates to enhance performance expectancy. Effort expectancy and social influence factors have direct positive influence on the use of social media systems in the government domain of Mongolia Research implications or Originality - This study empirically investigated the model of public employees' intention to examine the post-communist countries' cultural, social, economic, and political systems, government organisational environment of the former communist sphere. The cultural factors, censorship and organisational support, to the existing IT adoption UTAUT model were also identified to test the situation of a post-communist country, Mongolia. This study contributes to the new theoretical involvement with social media by testing a new social media-based third-party intercommunication channel, including intent to use in the public service for post-communist countries. This study practically provides the guidelines to promote social media usage for public sector in the post-communist situation.