International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
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Effects of $CoCl_2$ on Osteogenic Differentiation of Human Mesenchymal Stem Cells

  • Moon, Yeon-Hee (Dental Science Research Institute, School of Dentistry, Chonnam National University) ;
  • Son, Jung-Wan (Dental Science Research Institute, School of Dentistry, Chonnam National University) ;
  • Moon, Jung-Sun (Dental Science Research Institute, School of Dentistry, Chonnam National University) ;
  • Kang, Jee-Hae (Dental Science Research Institute, School of Dentistry, Chonnam National University) ;
  • Kim, Sun-Hun (Dental Science Research Institute, School of Dentistry, Chonnam National University) ;
  • Kim, Min-Seok (Dental Science Research Institute, School of Dentistry, Chonnam National University)
  • Received : 2013.07.15
  • Accepted : 2013.08.10
  • Published : 2013.09.30
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Abstract

Objective. To investigate the effects of the hypoxia inducible factor-1 (HIF-1) activation-mimicking agent cobalt chloride ($CoCl_2$) on the osteogenic differentiation of human mesenchymal stem cells (hMSCs) and elucidate the underlying molecular mechanisms. Study design. The dose and exposure periods for $CoCl_2$ in hMSCs were optimized by cell viability assays. After confirmation of $CoCl_2$-induced HIF-$1{\alpha}$ and vascular endothelial growth factor expression in these cells by RT-PCR, the effects of temporary preconditioning with $CoCl_2$ on hMSC osteogenic differentiation were evaluated by RT-PCR analysis of osteogenic gene expression, an alkaline phosphatase (ALP) activity assay and by alizarin red S staining. Results. Variable $CoCl_2$ dosages (up to $500{\mu}M$) and exposure times (up to 7 days) on hMSC had little effect on hMSC survival. After $CoCl_2$ treatment of hMSCs at $100{\mu}M$ for 24 or 48 hours, followed by culture in osteogenic differentiating media, several osteogenic markers such as Runx-2, osteocalcin and osteopontin, bone sialoprotein mRNA expression level were found to be up-regulated. Moreover, ALP activity was increased in these treated cells in which an accelerated osteogenic capacity was also verified by alizarin red S staining. Conclusions. The osteogenic differentiation potential of hMSCs could be preserved and even enhanced by $CoCl_2$ treatment.

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References

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