• Title/Summary/Keyword: pre-treatment method

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Sample pre-treatment for measurement of $^{129}$I in radwastes (방사성폐기물 중 $^{129}$I 측정을 위한 시료의 전처리)

  • Ke Chon Choi;Sun Ho Han;Jee Kwang Yong;Ki Seop Choi
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.3 no.1
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    • pp.49-56
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    • 2005
  • Many different kinds of radwastes are discharged from the nuclear power plants, and $^{129}$I is included in these radwastes. Recovery test of $^{129}$I was evaluated for different radwastes(dry active waste, sludge, spent resin and simulated evaporator bottom). Recovery of $^{129}$I for dry active waste by acid leaching with $1.8\%$ NaClO was $74.3\%$$(RSD,\;2.2\%)$ and l291 for spent rein by alkali fusion method with KOH as a flux agent was $87.7\%$$(RSD,\;0.9\%$), respectively. iodide in simulated evaporator bottom containing a high concentration of borate was adsorbed with anion exchange resin at pH 7 phosphate buffer solution. Recovery of $^{129}$I for anion exchange resin was $92.5\%$ and not affected up to 1,200 $\mu$g/mL $H_3BO)3$(as a Boron). Recovery of $^{129}$I for the spent resin from nuclear power plant was $87.2\%$ $(RSD,\;1.2\%)$.

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Anti-inflammatory Effects of Quercetin and Vitexin on Activated Human Peripheral Blood Neutrophils - The effects of quercetin and vitexin on human neutrophils -

  • Nikfarjam, Bahareh Abd;Hajiali, Farid;Adineh, Mohtaram;Nassiri-Asl, Marjan
    • Journal of Pharmacopuncture
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    • v.20 no.2
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    • pp.127-131
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    • 2017
  • Objectives: Polymorphonuclear neutrophils (PMNs) constitute the first line of defense against invading microbial pathogens. Early events in inflammation involve the recruitment of neutrophils to the site of injury or damage where changes in intracellular calcium can cause the activation of pro-inflammatory mediators from neutrophils including superoxide generation, degranulation and release of myeloperoxidase (MPO), productions of interleukin (IL)-8 and tumor necrosis factor ${\alpha}$ ($TNF-{\alpha}$), and adhesion to the vascular endothelium. To address the anti-inflammatory role of flavonoids, in the present study, we investigated the effects of the flavonoids quercetin and vitexin on the stimulus-induced nitric oxide (NO), $TNF-{\alpha}$, and MPO productions in human neutrophils. Methods: Human peripheral blood neutrophils were isolated, and their viabilities were determined by using the Trypan Blue exclusion test. The polymorphonuclear leukocyte (PMNL) preparations contained more than 98% neutrophils as determined by morphological examination with Giemsa staining. The viabilities of cultured neutrophils with various concentrations of quercetin and vitexin ($1-100{\mu}M$) were studied using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assays. Neutrophils were cultured in complete Roswell Park Memorial Institute (RPMI) medium, pre-incubated with or without quercetin and vitexin ($25{\mu}M$) for 45 min, and stimulated with phorbol 12-myristate 13-acetate (PMA) ($10^{-7}M$). NO production was carried out through nitrite determination by using the Griess method. Also, the $TNF-{\alpha}$ and the MPO productions were measured using enzyme-linked immunosorbent assay (ELISA) kits and MPO assay kits. Results: Neutrophil viability was not affected up to a concentration of $100{\mu}M$ of quercetin or vitexin. Both quercetin and vitexin significantly inhibited $TNF-{\alpha}$, NO, and MPO productions in human neutrophils (P < 0.001). Conclusion:The present study showed that both quercetin and vitexin had significant anti-inflammatory effects. Thus, treatment with either quercetin or vitexin may be considered as a therapeutic strategy for treating patients with neutrophil-mediated inflammatory diseases.

Analysis of Liquiritigenin, an Aglycone of Liquiritin in Licorice by High Performance Liquid Chromatography (감초 중 리퀴리티게닌 분석법 개발 및 함량분석)

  • Lee, Jong-Hwa;Ze, Keum-Ryon;Kim, Do-Hoon;Park, Ju-Young;Shim, Young-Hoon;Kim, Jong-Hwan;Lim, Sook;Shin, Jin-Seon;Kim, In-Seon;Kim, Ji-Yeon;Seong, Sang-Hyun;Jang, Seung-Yeup;Kim, Dong-Seup;Seong, Rack-Seon
    • Korean Journal of Pharmacognosy
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    • v.40 no.4
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    • pp.309-314
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    • 2009
  • Licorice(Glycyrrhizae Radix et Rhizoma) is recorded as the root of Glycyrrhiza uralensis Fischer or Glycyrrhiza glabra Linne or Glycyrrhiza inflata Bat.(Leguminosae) in Korean Pharmacopoeia $9^{th}$ edition (KP9) and Chinese Pharmacopoeia 2005(CP2005), Glycyrrhiza uralensis Fischer or Glycyrrhiza glabra Linne in Japanese Pharmacopoeia 2005(JP2005). It is established the content standard of Glycyrrhizin 2.5 % and liquiritin 1% in KP9 and CP2005. But, according to the reports, all Licorice species were not sufficient for content standard of liquiritin 1.0% for licorice in KP9 and CP2005. It shows different content of liquiritin among G. uralensis, G. glabra and G. inflata. Also, it was reported liquiritin, liquiritin apioside are transformed into liquiritigenin by human internal flora. Therefore, we have studied for the pre-treatment condition and analytical method of liquiritigenin; It was good efficinet in 2M HCl reflux(1 hr) for hydrolysis and in methylene chloride for solvent fractionation. And 1% acetic acid in DW(A) and acetonitrile(B) with gradient condition as a mobile phase was most effective in HPLC analytical condition. According to these experimental methods, we have anlayzed content of liquiritigenin about 77 Licorice sample. In this research, it was also examined the content of liquiritin and liquiritigenin for Glycyrrhizae Radix related growing area. According to the results, we suggested the content standard of glycyrrhizin more than 2.5%, liquiritigenin more than 0.7%(after hydrolysis) of licorice.

Thoracoscopic Talc Pleurodesis of Malignant Pleural Effusion (악성늑막삼출증의 흉강경 탈크늑막유착술)

  • 손동섭;기양수
    • Journal of Chest Surgery
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    • v.31 no.3
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    • pp.266-270
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    • 1998
  • The management of malignant pleural effusion is a difficult problem. Tetracycline was probably the most commonly used sclerosing agent, but parenteral tetracycline is no longer available. This prospective study was designed to determine the efficacy of insufflated talc in producing plerodesisin patients with malignant pleural effusions. Fifteen patients managed between March 1995 and March 1997 underwent thoracoscopy and had 4 gm sterile asbestos-free talc insufflation throughout the pleural space. The mean interval between the procedure and last follow-up was 101 days(range: 25-233 days). The results of treatment were clinically and radiologically compared with pre-operative status. The results were as follows: 1. The patients were 6 men and 9 women with a mean age of 55.1 years(range: 31-74 years). The primary tumors were lung cancer in 10 patients, breast cancer in two, hepatoma in one, stomach cancer in one, and adenocarcinoma of unkown origin in one. 2. Cancer cell positive ratio was 36% in sputum cytology study and 100% in thoracoscopic biopsy. 3. Symptoms improved in 94% of patients treated by talc pleurodesis. 4. After 30 days of the procedure, there was radiologically no recurrence of plural fluid in 94% patients. 5. There were wound infection in one, respiratory failure in one. In conclusion, the insufflation of 4 gm sterile asbestos-free talc in the pleural space is an effective method to control malignant pleural effusions and patients selection is important factor for reexpansion the lung.

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Quantitative Analysis of Antioxidants in Korean Pomegranate Husk (Granati pericarpium) Cultivated in Different Site (HPLC에 의한 산지별 한국산 석류과피 중 항산화화합물의 함량분석)

  • Kwak, Hye-Min;Jeong, Hyun-Hee;Song, Bang-Ho;Kim, Jong-Guk;Lee, Jin-Man;Hur, Jong-Moon;Song, Kyung-Sik
    • Applied Biological Chemistry
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    • v.48 no.4
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    • pp.431-434
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    • 2005
  • The quantitative analytical method for major antioxidants, ellagic acid and punicalagin, in pomegranate husk (Granati pericarpium) were established by HPLC. The optimal HPLC conditions were as follows: Column; Agilent Zorbax Eclipse XDB-C18 ($4.6{\times}150mm,\;5{\mu}m$), mobile phase; 1% formic acid in water (A) and 1% formic acid in MeCN (B) (gradient elution of 5% to 100% B for 50 min), flow rate; 0.8 ml/min., detection; UV 254 nm. The optimal pre-treatment conditions for HPLC analysis were as follows: 5 g of pomegranate husk in 100 ml of 95% EtOH, refluxed for 3 h. Under these analytical conditions, punicalagin and ellagic acid contents in Korean pomegranates husks which were cultivated in five different sites were determined. As results, the ellagic acid and punicalagin (as a mixture of ${\alpha-\;and\;{\beta}-anomer$) contents were the highest in Haepyung pomegranate husk $(15.27{\mu}g/mg)$ and Jangsung pomegranate husk $(16.21{\mu}g/mg)$, respectively.

A Comparative Study on the Rules of Origin and Origin Implementation Procedure in KORCHINA FTA and Main Korea's Existing FTAs - Focused on KORUS·KOREU·KORASEAN FTA - (한·중 FTA와 기(旣)체결 주요 FTA의 원산지 규정과 절차 비교연구 - 미국·EU·ASEAN FTA 중심으로 -)

  • LIM, Mok-Sam;LIM, Sung-Chul
    • THE INTERNATIONAL COMMERCE & LAW REVIEW
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    • v.69
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    • pp.589-616
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    • 2016
  • The reviewing of an analysis of the Korea-China FTA due to guidance introduced for the new regulations or exceptional regulations compared to the KORUS, Korea-EU, Korea-ASEAN FTA. Commodity sectors in the Korea-China FTA and the KORUS, Korea-EU, Korea-ASEAN FTA(the majority in the country and trade criteria analysis result) compared and analyzed the results, rules of origin and the customs clearance procedures of origin, preferential tariff rate of origin and the origin preferential specific rules are somewhat difference, but customs and trade facilitation regulations are already quite consistent with the Korea customs system. Relatively important research results were as follows. First, the calculation of the regional value content in KORCHINA FTA is that I'm to use the deduction method can comprehensively reflect a regional value ratio, with respect to the materials acquired originating status as the FTA in the US and EU use the product non it's not to consider the value of the originating materials originating materials can be utilized for intermediate goods. Second, even if a non-treaty country in the middle with the exception of direct transport rules, and acknowledge the country of origin are under customs control, there are provisions for the period are temporarily stored in a non-treaty countries separately, that period goods imported into the non-treaty countries and up to three months from the day. If the situation of the occurrence of force majeure be greater than three months, but has so exceed six months. Third, the materials acquired originating status in the Korea-China FTA not to consider the value of non-originating materials used in its products as the KORUS FTA and Korea-EU FTA, that can be utilized originating materials for intermediate goods. It is expected that higher utilization of rules of origin. Meanwhile, Korea-China FTA has provisions to allow requests for preferential tariff applied on imports Customs declaration of intention to apply pre-condition for a preferential tariff applied to the importer. In other words, if the import customs tariff preference when applying post-intention not to advance is to be noted that any preferential treatment to prevent the later application.

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Fact-finding survey on herbicide use in paddy rice at farmer's level (논 제초제 사용에 대한 농가실태조사)

  • Lee, In-Yong;Park, Jae-Eup;Park, Tae-Seon;Kim, Tae-Wan;Oh, Byung-Youl
    • The Korean Journal of Pesticide Science
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    • v.2 no.2
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    • pp.119-125
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    • 1998
  • A nation-wide fact-finding survey was done to provide the basic information for establishing the rational weed control scheme in paddy rice. A total of 445 respondents including 273 farmers and 172 pesticide dealers was involved in this survey. Dominant weeds in rice paddy field were Echinochloa crus-galli, Eleocharis kuroguwai, Sagittaria pygmaea, Sagittaria trifolia, etc.. Preferred herbicides widely used by farmners were ranked in the following order; butachlor 5GR, pyrazosulfuron-ethyl + molinate 5.07GR, molinate + cinosulfuron 5.08GR, meanwhile, those recommended by pesticide dealers were; pyrazosulfuron-ethyl + molinate 5.07GR, molinate + cinosulfuron 5.08GR, and butachlor 5GR. The guidelines to choose the preferred herbicides was primarily based on the experience, which was occupied about 45%. Approximately 56% of the farmers followed the recommended herbicide dosage and 43% of them applied the herbicides before weed germination (pre-emergence treatment). In addition, most farmers applied herbicides at least two times with systematic application schedule. Application method of granular formulation was broadcasting by hand, while that of sprayable ones was by high-pressure sprayer. Some farmers answered that they had experienced phytotoxicity in the crop by applied herbicides, of which higher experience was observed from the farmers who applied by high-pressure sprayer. It was analyzed significantly that the feasible herbicides to cause phytotoxicity in rice at farmer's level were butachlor 5GR, esprocarb + pyrazosulfuron-ethyl 5.07GR and bensulfuron-methyl + molinate 5.17 GR.

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An The Effects of Backyard Gardening Activities of Children's Nutrition Knowledge and Eating Habits (텃밭가꾸기 활동이 유아의 영양지식 및 식습관에 미치는 영향)

  • Koo, Ja-Lim;Han, Sang-Kil
    • The Journal of the Korea Contents Association
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    • v.20 no.7
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    • pp.292-301
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    • 2020
  • This study explored the changes in nutrition knowledge and eating habits formation of infants through vegetable gardening activities in early childhood education institutions. children from H nursery school located in Suwon-si, Gyeonggi-do, and H nursery school located in Hwaseong-si, Gyeonggi-do, were divided into 18 experimental groups and 18 people from comparison group and pre-post analysis was conducted. The research tool analyzed the data collected using the nutrition knowledge measuring tool and the eating habits measuring tool using the program SPSS, and the analysis method was conducted with t-test(verification) to verify the homogeneity of the experimental group and the comparative group. Analysis result, first. Infants aged 3 to 4 in experimental groups who have experienced vegetable gardening activities have higher nutritional knowledge than infants in comparison groups. In the case of nutrition knowledge, there was no statistically significant difference between the experimental group and the comparative group before the vegetable gardening activities were carried out. But after the experimental treatment, the experimental group showed statistically significant differences in improving the concept of nutritional knowledge (the reason for eating food, the type and function of food, hygiene, and garden) in the post-examination of the comparative group. Second, it can be seen that infants aged 3 to 4 in experimental groups who experienced vegetable gardening activities showed significant differences in table manners and eating behavior among sub-factors of eating habits compared to infants in comparison groups who had never experienced them. In conclusion, vegetable gardening activities are educationally useful in terms of expecting positive changes in the nutrition knowledge and eating habits formation of infants.

Historian Samuel Eliot Morison and Writing History of United States Naval Operations in World War II (해양사가 새뮤얼 엘리엇 모리슨(Samuel Eliot Morison)의 해전사 서술과 그 현대적 의미 - 『제2차 세계대전기 미국 해군 작전사』를 중심으로 -)

  • Kim, Hyun-Seung
    • Strategy21
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    • s.42
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    • pp.53-82
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    • 2017
  • Samuel Eliot Morison (1887-1976) was one of the pre-eminent historians of his generation. He was not only a famous historian at that time, but also was promoted to the rank of Admiral in U.S. Navy Reserve. Fifteen volume History of United States Naval Operations in World War II was published between 1947 and 1962, was not only a comprehensive report on the Navy's projection of power over two oceans, but a classic of historical literature that stands as the definitive treatment of it subject. Although he was fifty-five when war come to America in December 1941. Samuel Eliot Morison was determined to play a role. A professor at Harvard at the time, he joined volunteering for duty in the Navy. An experienced sailor, Professor Morison had earlier sailed that same routes taken by Christopher Columbus while researching his biography, Admiral of the Ocean Sea, which appeared in January 1942 th much acclaim and later got a Pulitzer Prize. Thus Morison plunged into the war, crossing the Atlantic aboard a destroyer. He assumed himself as "Parkman on the sea", tried to follow Parkman's historiographic method, not only participatory history but also literary style. And during writing History of United States Naval Operations in World War II, He emphasized two principles, publicity and objectivity. In terms of publicity, he always worried about who read history and why. In his pamphlet, "History as a Literary Art", he asserted it is useless if readers do not read a history which historians wrote. So he thought historians have forgotten that there is an art of writing of history. Therefore, he built his narratives around brightly rendered visuals and used the present tense to describe actions he witnessed firsthand, he wrote of the U.S. combat in very vividly. But strongly driven by publicity, he sometimes lost his balance in writing the naval history. For instance, the naval history became the focus of criticism for its prejudiced comments about the commanders. Also some reviewers asserted he did not secure the objectivity on writing the naval history. Although he sometimes deliberately torpedoed the objectivity of his work for strengthening publicity, by writing an extensive U.S. naval history, he introduced maritime history and naval history to the public widely. Until in early twentieth century, U.S. historians usually had been focusing their effort to the traditional areas, for example politic, economy, and etc. His intensive effort on the operations of U.S. Navy in World War II aroused a public interest in maritime and naval history. In conclusion, through using literary style and realistic narratives, historian Morison wrote a naval history for all the people which could appealed to the public.

Gas Chromatographic Performances for Simultaneous Determination of Multi-pesticide Residues and Extraction of Pesticides with Three Partition Solvents (잔류농약 다성분 동시분석을 위한 기체 크로마토그래피 분석성능과 3종 분배용매에 의한 농약추출)

  • Kim, Chan-Sub;Kim, Jin-Bae;Im, Geon-Jae;Park, Hyun-Ju;Lee, Young-Deuk
    • The Korean Journal of Pesticide Science
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    • v.13 no.3
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    • pp.133-147
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    • 2009
  • In order to develop the simultaneous analytical method for pesticide residues which ones in soil are rapidly and inexpensively measured by, many analytical methods for individual pesticide residue and several methods for multi-pesticide residues in agricultural commodities and food were reviewed. Various gas chromatographic conditions and pre-treatment procedures were intensively investigated and modified. And then new optimum procedure was established and its proficiency was validated. The response on detectors of simultaneous determination of 180 pesticides out of pesticides used in Korea was tested. The number of pesticides that their limits of detection (LOD) equal or lower than $0.05\;mg\;kg^{-1}$ were 137 pesticides on ECD, 170 pesticides on NPD and 179 pesticides on ECD/NPD integrated. The retention times of most pesticides ranged appropriately between 10 minutes and 40 minutes in the proposed chromatographic conditions. But about 90 pesticides eluded between 20 minutes and 30 minutes and so they were not resolved enough. In partition with dichloromethane, ethyl acetate/hexane (1:1) and ethyl acetate, number of pesticides whose extraction efficiency ranged from 70% to 120%, were 163, 154 and 147, respectively.