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Quantitative Analysis of Antioxidants in Korean Pomegranate Husk (Granati pericarpium) Cultivated in Different Site  

Kwak, Hye-Min (Division of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Kyungpook National University)
Jeong, Hyun-Hee (Division of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Kyungpook National University)
Song, Bang-Ho (College of Teachers, Kyungpook National University)
Kim, Jong-Guk (School of Life Sciences & Biotechnology, Kyungpook National University)
Lee, Jin-Man (Department of Herb & Food Science, Kyungpook college of Sciences)
Hur, Jong-Moon (Division of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Kyungpook National University)
Song, Kyung-Sik (Division of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Kyungpook National University)
Publication Information
Applied Biological Chemistry / v.48, no.4, 2005 , pp. 431-434 More about this Journal
Abstract
The quantitative analytical method for major antioxidants, ellagic acid and punicalagin, in pomegranate husk (Granati pericarpium) were established by HPLC. The optimal HPLC conditions were as follows: Column; Agilent Zorbax Eclipse XDB-C18 ($4.6{\times}150mm,\;5{\mu}m$), mobile phase; 1% formic acid in water (A) and 1% formic acid in MeCN (B) (gradient elution of 5% to 100% B for 50 min), flow rate; 0.8 ml/min., detection; UV 254 nm. The optimal pre-treatment conditions for HPLC analysis were as follows: 5 g of pomegranate husk in 100 ml of 95% EtOH, refluxed for 3 h. Under these analytical conditions, punicalagin and ellagic acid contents in Korean pomegranates husks which were cultivated in five different sites were determined. As results, the ellagic acid and punicalagin (as a mixture of ${\alpha-\;and\;{\beta}-anomer$) contents were the highest in Haepyung pomegranate husk $(15.27{\mu}g/mg)$ and Jangsung pomegranate husk $(16.21{\mu}g/mg)$, respectively.
Keywords
antioxidant; ellagic acid; punicalagin; quantitative analysis; pomegranate husk;
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1 Zwart, L. L., Meerman, J. H. N., Commander, N. M. and Vemeulen, N. P. E. (1996) Biomarkers of free radical damage applications in experimental animals and humans. Free Radical Biol. Med. 26, 202-226   DOI   ScienceOn
2 Halliwell, B., Murcia, M. A., Chirico, S. and Aruoma, O. I. (1995) Free radicals and antioxidants in food and vivo: what they do and how they work. Crit. Rev. Food Sci. Nutr. 35, 7-20   DOI   ScienceOn
3 Meydani, S. N., Wu, D., Santos, M. S. and Hayek, M. (1995) Antioxidants and immune response in aged persons: overview of present evidence. Am. J. Clin. Nutr. 62, 14625-14765
4 Chang, S. T., Wu, J. H., Wang, S. Y., Kang, P. L., Yang, N. S. and Shyur, L. F. (2001) Antioxidant activity of extracts from Acacia confusa bark and heartwood. J. Agric. Food Chem. 49, 3420-3424   DOI   ScienceOn
5 Schubert, S. Y., Lansky, E. P. and Neeman, I. (1999) Antioxidant and eicosanoid enzyme inhibition properties of pomegranate seed oil and fermented juice flavonoids. J. Ethnopharmacol. 66, 11-17   DOI   ScienceOn
6 Gil, M. I., Tomas-Barberan, F. A, Hess-Pierce, B., Holcroft, D. M. and Kader, A. A. (2000) Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. J. Agric. Food Chem. 48, 4581-4589   DOI   ScienceOn
7 Oyangui, Y. (1989) SOD and active oxygen modulators. Nihon Igakukan, Tokyo, pp. 17
8 Wang, S. Y. and Jiao, H. (2000) Scavenging capacity of berry crops on superoxide radicals, hydrogen peroxide, hydrogen peroxide, hydroxly radicals, and singlet oxygen. J. Agric. Food Chem. 48, 5677-5684   DOI   ScienceOn
9 Bengendi, L., Benes, L., Durackova, Z. and Ferencik, M. (1999) Chemistry, physiology and pathology of free radicals. Life Sci. 65, 1864-1874
10 Nakayama, T., Niimi, T., Osawa, T. and Kawakishi, S. (1992) The protective role of polyphenols in cytotoxicity of hydrogen peroxide. Mutat. Res. 281, 77-80   DOI   ScienceOn
11 Shim, S.-M., Choi, S.-W. and Bae, S.-J. (2001) Effects of Punica granatum L. fractions on quinone reductase induction and growth inhibition on several cancer cells. J. Kor. Soc. Food Sci. Nutr. 30, 80-85
12 Steinberg, D. (1991) Antioxidants and atherosclerosis: a current assessment. Circulation 84, 1420-1425
13 Poyrazogiu, E., Gokmen, V. and Atrik, N. (2002) Organic Acids and Phenolic Compound in Pomegranates (Punica granatum L.) Grown in Turkey. J. Food Composit. Anal. 15, 567-575
14 McCord, J. M. and Fridovich, I. (1969) Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein). J. Biol. Chem. 244, 6049-6055
15 Hamilton, R. J., Kalu, C., Prisk, E., Padley, F. B. and Pierce, H. (1997) Chemistry of free radicals in lipids. Food Chem. 60, 193-199   DOI   ScienceOn
16 Lu, Y., Foo, Y. (2001) Antioxidant activities of polyphenols from sage (Salvia ofjicinals). Food Chem. 75, 197-202   DOI   ScienceOn
17 Lodovici, M., Guglielmi, F., Meoni, M. and Dolara, P. (2001) Effect of natural phenolic acids on DNA oxidation in vitro. Food Chem. Toxicol. 39, 1205-1210   DOI   ScienceOn
18 Proctor, P. H. (1992) Free radicals and human disease. In Handbook of free radicals and antioxidants in medicine. Vol. I, CRC Press, Boca Raton, FL. pp. 17
19 Ames, B. M., Shigena, M. K., Hagen, T. M. (1993) Oxidants, antioxidants and the degenerative diseases of aging. Proc. Natl. Acad. Sci. 90, 7915-7922   DOI   ScienceOn
20 Gackowski, D., Kruszewski, M., Jawien, A., Ciecierski, M. and Olinski, R. (2001) Further evidence that oxidant stress may be a risk fator responsible for the development of atherosclerosis. Free Radical Biol. Med. 31, 542-547   DOI   ScienceOn
21 Kwak, H.-M., Jeon, S.-Y., Sohng, B.-H., Kim, J.-K, Lee, J.-M., Lee, K-B., Jeong, H.-H., Hur, J.-M. (2005) $\beta$-Secretase (BACEl) inhibitors from pomegranate peel. Arch. Pharm. Res. submitted
22 Lodovici, M., Guglielmi, F., Meoni, M. and Dolara, P. (2001) Effect of natural phenolic acids on DNA oxidation in vitro. Food Chem. Toxicol. 39, 1205-1210   DOI   ScienceOn
23 Burda, S. and Oleszaw, W. (2001) Antioxidant and antiradical activities of flavonids. J. Agric. Food Chem. 49, 2774-2779   DOI   ScienceOn
24 Hollman, P. C. H. and Katan, M. B. (1999) Dietary flavonoids: intake, health effects and bioavailalility. Food Chem. Toxicol. 73, 937-942
25 Noda, Y., Kaneyuki, T., Mori, A and Packer, L. Antioxidant activities of pomegranate fruit extract and its anthocyanidins: delphinidin, cyanidin, and pelagonidin. J. Agric. Food Chem. 50, 166-171   DOI   ScienceOn
26 Aviram, M., Dornfeld, L., Rosenblat, M., Volkova, N., Kaplan, M., Coleman, R., Hayek, T., Presser, D. and Fuhrman, B. (2000) Pomegranate juice consumption reduces oxidative stress, atherogenic modifications to LDL, and platelet aggregation: studies in humans and in atherosclerotic apolipoprotein E-deficient mice. Am. J. Clin Nutr. 71, 1062-1076
27 Fuhrman, B., Volkova, N., Rosenblat, M. and Aviram, M. (2000) Lycopene synergistically inhibits LDL oxidation in combination with vitamin E, glabridin, rosmarinic acid, carnisic acid, or galic. Antioxidants Redox Signaling 2, 491-506   DOI   ScienceOn
28 Koh, J.-H., Hwang, M.-O., Moon, J.-S., Hwang, S.-Y. and Son, J.- Y. (2005) Antioxidative and antimicrobial activities of pomegranate seed extract. Kor. J. Food Cookery Sci. 21, 171-179
29 Singh, R., Murthy, K. C. and Jayaprakasha, G. (2002) Studies on the antioxidant activity of pomegranate (P. granatum) peel and seed extract using in vitro models. Agric. Food Chem. 50, 81-86   DOI   ScienceOn