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Quantitative Analysis of Antioxidants in Korean Pomegranate Husk (Granati pericarpium) Cultivated in Different Site  

Kwak, Hye-Min (Division of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Kyungpook National University)
Jeong, Hyun-Hee (Division of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Kyungpook National University)
Song, Bang-Ho (College of Teachers, Kyungpook National University)
Kim, Jong-Guk (School of Life Sciences & Biotechnology, Kyungpook National University)
Lee, Jin-Man (Department of Herb & Food Science, Kyungpook college of Sciences)
Hur, Jong-Moon (Division of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Kyungpook National University)
Song, Kyung-Sik (Division of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Kyungpook National University)
Publication Information
Applied Biological Chemistry / v.48, no.4, 2005 , pp. 431-434 More about this Journal
Abstract
The quantitative analytical method for major antioxidants, ellagic acid and punicalagin, in pomegranate husk (Granati pericarpium) were established by HPLC. The optimal HPLC conditions were as follows: Column; Agilent Zorbax Eclipse XDB-C18 ($4.6{\times}150mm,\;5{\mu}m$), mobile phase; 1% formic acid in water (A) and 1% formic acid in MeCN (B) (gradient elution of 5% to 100% B for 50 min), flow rate; 0.8 ml/min., detection; UV 254 nm. The optimal pre-treatment conditions for HPLC analysis were as follows: 5 g of pomegranate husk in 100 ml of 95% EtOH, refluxed for 3 h. Under these analytical conditions, punicalagin and ellagic acid contents in Korean pomegranates husks which were cultivated in five different sites were determined. As results, the ellagic acid and punicalagin (as a mixture of ${\alpha-\;and\;{\beta}-anomer$) contents were the highest in Haepyung pomegranate husk $(15.27{\mu}g/mg)$ and Jangsung pomegranate husk $(16.21{\mu}g/mg)$, respectively.
Keywords
antioxidant; ellagic acid; punicalagin; quantitative analysis; pomegranate husk;
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