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Antimicrobial Plant Extracts as an Alternative of Chemical Preservative: Preservative Efficacy of Terminalia chebula, Rhus japonica (gallut) and Cinnmomum cassia Extract in the Cosmetic Formular (가자, 오배자, 계피 추출물을 이용한 화장품 제형에서의 방부효과)

  • Cho, Eun-Mi;Bae, Jun-Tae;Pyo, Hyeong-Bae;Lee, Geun-Su
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.34 no.4
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    • pp.325-331
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    • 2008
  • This study was carried to investigate the efficiency of antimicrobial plant extracts as natural preservative in the cosmetic formulations. Ethanol extracts of different plants were tested using the disc diffusion (paper disc) method and the minimum inhibitory concentration (MIC) method for their antimicrobial activity against the common poultry pathogens. Terminalia chebula and Rhus japonica (gallut) extracts exhibited antibacterial activity against Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli. Cinnmomum cassia extract exhibited antifungal activity against Candida albicans and Aspergillus niger while the remaining plant extracts showed no activity. A study of the preservative efficacy of the cosmetic formular containing the T. chebula, R. japonica and C. cassia extracts demonstrated sufficient preservative efficacy against bacteria and eukaryotic test microbes. Also, the cosmetic formulations containing antimicrobial plant extracts more effectively inhibited the microoranisms than the mixture of traditional chemical preservatives. These results suggest that the mixture of antimicrobial plant extracts, T. chebula, R. japonica and C. cassia is incorporated as preservative in the cosmetic formulation and the mixture have considerable effect on its efficacy.

Seasonal Dynamics of Enzymetic Activities and Functional Diversity in Soils under Different Organic Managements (시용 유기물을 달리한 토양에서 미생물 군락의 효소활성과 기능적 다양성의 계절적 변화)

  • Park, Kee-Choon;Kremer, Robert J.
    • Korean Journal of Soil Science and Fertilizer
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    • v.42 no.4
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    • pp.307-316
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    • 2009
  • Soil microbial activity and diversity are affected by organic sources applied to improve soil quality and fluctuate seasonally. We investigated the effects of municipal compost (MC), poultry litter (PL), and cover crops of spring oats and red clover (RC) on soil enzyme activities, and soil bacterial community-level physiological profiling (CLPP) in a Mexico silt loam in North Central Missouri, USA. Temporal patterns of these parameters were observed by periodic five soil sampling from spring to fall over a two year period. MC increased soil dehydrogenase (DH) activity consistently beginning about three months after MC application; fluorescein diacetate (FDA) hydrolytic activity significantly began to increase by the September of the first year but fluctuated during the following period. DH activity responded more directly to the amount or properties of organic residues in soils while FDA hydrolysis and CLPP were generally influenced by composition of organic sources, and enzyme activities and CLPP showed seasonal variation, which depended on organic sources and soil moisture. MC and cover crops may be useful organic sources for enhancing general soil microbial activity and altering soil microbial diversity, respectively. Because microbial activities and diversity are dynamic and subject to seasonal changes, the effects of organic amendments on these parameters should be investigated frequently during a growing season.

Effects of a Biological Amendment on Chemical and Biological Properties and Microbial Diversity in Soils Receiving Different Organic Amendments (각기 다른 유기물이 투여된 토양에서 토양의 화학적, 미생물학적 특성과 미생물의 다양성에 미치는 생물비료의 효과)

  • Park, Kee-Choon;Kremer, Robert J.
    • Korean Journal of Soil Science and Fertilizer
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    • v.40 no.4
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    • pp.234-241
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    • 2007
  • Biological amendments consisting of suspensions of selected microorganisms are often used in conjunction with various organic materials for amending soils to improve soil quality and plant growth. The effects of the biological amendment on chemical and biological properties of soil were investigated for a biological amendmentalone and when combined with different organic materials includingmunicipal compost (MC), poultry litter (PL), and cover crops (red clover (RC) and spring oats). A liquid preparation of a biological amendment called Effective Microorganisms was sprayed on the tested plots three times over a two-year period. Effective Microorganisms alone did not influence pH, K, or organic matter content in soil. However, increases in P in PL-treated soils in fall of both years andCa in MC-treated soil in fall 2001, and decreases in Ca, Mg, and cation exchange capacity (CEC) in RC-planted soil were associated with EM. Increased dehydrogenase(DH) activitiesassociated with Effective Microorganismswere only detected in July (P=0.0222) and October (P=0.0834) for RC-planted soils in the first year. Fluorescein diacetate (FDA) hydrolysisappeared to be enhanced by Effective Microorganisms in soils untreated or treated with MC and oatsbut only sporadically during the sampling period. FDA hydrolysis in both PL- and RC-treated soils as well as DH activity in PL-treated soils decreased with Effective Microorganisms treatment. Effective Microorganisms did not influence substrate utilization patterns expressed by the BIOLOG assay. We conclude that Effective Microorganisms effects on soil chemical and biological properties varied depending on the added organic materials. Effective Microorganisms periodically increased soil DH activity and FDA hydrolysis with RC and with MC plus oats, respectively.

Microbial Inactivation of Chicken Cage Litter by Aqueous Chloride Dioxide (이산화염소수를 이용한 계사 내 깔짚의 미생물 수 저감화)

  • Yu, Dong-Jin;Kim, Hyun-Jin;Song, Hyeon-Jeong;Shin, Yoon-Ji;Chae, Hyun-Seok;Song, Kyung-Bin
    • Food Science and Preservation
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    • v.18 no.1
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    • pp.98-102
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    • 2011
  • We evaluated microbial inactivation in chicken cage litter, to ensure microbial safety, using aqueous chloride dioxide. Contamination by coliforms, Escherichia coli, Listeria spp., yeasts and molds, total aerobic bacteria, and Salmonella spp. was detected in fresh cage litter, and microbial populations increased if litters were repeatedly used. Aqueous $ClO_2$ treatment (500 ppm) significantly decreased the populations of coliforms, E. coli, Listeria spp., yeasts and molds, total aerobic bacteria, and Salmonella spp. in all litter samples tested. In particular, aqueous $ClO_2$ treatment on fresh litter reduced the initial populations of coliform, E. coli, Listeria spp., yeasts and molds, and total aerobic bacteria by 4.47, 1.29, 1.23, 3.24, and 5.2 log CFU/g, respectively. In addition, when litters used for 1 and 5 weeks were tested, treatment significantly reduced microbial populations. The results suggest that aqueous $ClO_2$ treatment is useful to reduce microbial hazards in chicken cage litter and to improve the microbial safety of slaughtered chickens.

Acaricidal Activity of (E) - Anethole Derived from Illicium verum and Its Analogues against Dermanyssua gallinae Adults (닭진드기 성충에 대한 대회향 유래 아네톨 및 유사 화합물들의 살비활성)

  • Jo, Hyeong-Chan
    • Korean journal of applied entomology
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    • v.48 no.2
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    • pp.263-268
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    • 2009
  • The acaricidal activities of Illicium verum fruit-derived materials against adults of Dermanyssus gallinae were examined using the direct contact application method. Based on laboratory tests, an acaricidal constituent of I. verum fruit was determined because of its potent activity. Results were compared with those of the currently used acaricides such as dichlorvos, diazinon, and carbaryl. The acaricidal principle of I. verum fruit was identified as (E)-anethole using a GC-MS. lts acaricidal activity was compared with those of 12 compounds having a similar chemical moiety. Based on the $LD_{50}$ values, the acaricidal activities of (+)-or-(-)-neomenthol were the strongest (0.01 $mg/cm^2$) and (E)-anethole, (+)-or-(-)-menthol, (${\pm}$)-isoborneol, (-)-menthone, and (lS)-endo-(-)-bomeol showed similar results (0.02 $mg/cm^2$), and (1R)-(+)-camphor and (+)-menthone also gave good activities (0.03 and 0.04 $mg/cm^2$, respectively). These compounds showed more toxic acaricidal activities than diazinon and carbaryl, 0.05 and > 0.2 $mg/cm^2$, respectively, but were not comparable to that of dichlorvos with 0.0002 $mg/cm^2$. These results indicate that the I. verum fruit-derived materials and tested compounds descried as poultry red mites-control agents could be useful for managing field populations of D. gallinae.

Identification of SNPs in Highly Variable Lysozyme Gene in Korean Native Chicken Populations (한국 재래닭의 고변이 Lysozyme 유전자의 SNP 확인)

  • Hoque, M.R.;Kang, B.S.;Lim, H.K.;Choi, K.D.;Lee, J.H.
    • Korean Journal of Agricultural Science
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    • v.37 no.3
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    • pp.399-404
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    • 2010
  • Single nucleotide polymorphisms (SNPs) in chicken lysozyme (LYZ) gene were investigated in this study. The identification of SNPs in both exon and intron in LYZ gene has led to understanding of evolution for the domestic chicken populations. A total of 24 samples from two Korean native commercial chicken populations (CCPs) were used for the initial identification of SNPs by mixing three DNA samples for sequencing experiments. By comparing with red jungle fowl (RJF), two commercial chicken populations have 18 common polymorphisms. Between two commercial chicken populations, 15 polymorphisms were identified. Of the 33 polymorphisms identified, two indels (21 and 4 bp) were found. Whereas, only one polymorphism in exon 2 at the bp position 1426 was a non-synonymous substitution (p.Ala49Val), indicating the amino acid changes. The identified non-synonymous substitution (p.Ala49Val) is located close to the catalytic sites of the enzyme, which might affect its activity. In our investigation, the polymorphisms in LYZ gene can provide broad ideas for the variation of Korean native chicken populations from the ancestor of chicken breeds as well as the some biological functions of the LYZ gene.

Dietary Habits Contributing to Breast Cancer Risk Among Iranian Women

  • Mobarakeh, Zahra Sheikhi;Mirzaei, Khadijeh;Hatmi, Nadia;Ebrahimi, Mandana;Dabiran, Sohaila;Sotoudeh, Gity
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.21
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    • pp.9543-9547
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    • 2014
  • Background: The aim of this study was to investigate demographic features, dietary habits, and some possible risk factors for being susceptible to breast cancer in Iranian women. Materials and Methods: A study of dietary habits and breast cancer was conducted among 53 Iranian women with histological confirmed disease and 40 matched controls. A dietary habits questionnaire was used to evaluate the pattern of selected food intakes. The risk of cancer was analyzed after adjustment for confounding factors. Age, weight, body mass index (BMI), waist circumference, educational status, parity, lactation, marital status, menopause, history of estrogen therapy, and family history of breast disease or cancer were assessed among participants. Special attention was given to the relationship between consumption of high fat meat, milk, yogurt and cheese as well use of frying oils for frying foods, use of olive/liquid oils for cooking, removing fat from meat and poultry, removing chicken skin and not use of mayonnaise as salad dressing and the risk of breast cancer. Moreover, salad, vegetable and fruit consumption, and eating outdoors owere investigated. Results: Our results revealed significant lower education and higher BMI and waist circumference levels in patients with breast cancer. There was significantly increased breast cancer risk in overweight women in comparison with normal weight (OR=2.91, 95%CI 1.24 to 6.82). High intake of fat dairy products including milk and cheese was found to be a statistically significant factor for increasing breast cancer risk in models adjusting for age, BMI and education. Use of olive/liquid oils for cooking and avoidance of mayonnaise as salad dressing are related to lower risk of breast cancer. The frequency of vegetable and fruit consumption was significantly lower in patients with breast cancer compared to healthy women. Conclusions: Dietary habits might be risk factors for breast cancer among Iranian women. Adoption of a prudent diet could be an appropriate strategy for preventing breast cancer.

Protein and Phosphorus Availabilities of Five Different Dietary Protein Sources in Juvenile Olive flounder (Paralichthys olivaceus) as Determined by Growth Performance and Phosphorus Retention

  • Choi, Se-Min;Kim, Kang-Woong;Wang, Xiaojie;Han, Kyung-Min;Bai, Sungchul C
    • Journal of Aquaculture
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    • v.16 no.2
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    • pp.104-109
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    • 2003
  • The present study aims to evaluate protein and phosphorus availabilities of five different dietary protein sources during the 6-week feeding trial in juvenile olive flounder, Paralichthys olivaceus as determined by growth performance and phosphorus retention. Five diets containing blood meal (BM), poultry by-product (PBP), squid liver powder (SLP), feather meal (FM) and soybean meal (SM) were prepared by mixing a basal diet (BD) with one of five test ingredients at the ratio of 7 to 3. As a reference diet, BD contains three different protein sources such as white fish meal, casein and gelatin. After 2 weeks of the conditioning period, fish initially averaging 2.7$\pm$0.02g (mean$\pm$SD) were randomly distributed into each aquarium as a group of 30 fish reared in the recirculating system. Fish of triplicate groups were fed one of six experimental diets (BD+five test diet). After 6-week feeding trial, pro- tein efficency ratio (PER) of fish fed BM diet was the lowest in experiment groups. While fish fed PBP diet showed a significant higher PER as compared to the FM diet, and fish fed SLP diet and BD were a higher PER than did fish fed PBP diet. However, there was no significant difference in PER among fish fed SLP diet, BD and SM diet, and between SM diet and PBP diet. Phosphorus retention efficiency of bone (PR $E_{b}$) of fish fed BM diet was the lowest in all the diets, and fish fed FM diet showed a higher PE $R_{b}$ than fish fed BD and SM diet. However, there was no significant difference in PER among fish fed FM diet, SLP diet and PBP diet, and among SLP diet, PBP diet, SM diet and BD. These results indicate that SLP could be a suitable protein source for low pollution diets of olive flounder in the future fish feeds market. Furthermore, PBP and SM are available protein source to reduce P waste in the oliver flounder aquaculture with the use of proper mixture of other protein sources and more processing to improve protein availibility of these.ese.

MASS PRODUCTION OF ROTIFERS FOR THE CULTURE OF FISH AND SOME SHRIMP LARVAE (은어 및 새우류의 유생 사육을 위한 Rotifer의 대량 배양)

  • KIM In-Bae
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.5 no.2
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    • pp.45-49
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    • 1972
  • The following are some results obtained from a series of experiments in rotifer culture and its usage for the food of tiny fish fry: 1, Outdoor concrete ponds, each being $16m^2$, were used to culture the rotifers, Brachionus calyciflorus, and Filinia longiseta. Brachionus calyciflorus usually attained the population of about 100 individuals per ml of pond water. Dipterex was usually applied to control Daphni,a and other crustaceans that generally appear and feed on rotifers. A concentration of 0.16 to 0.2 ppm in the pond water was sufficiently effective to control these natural enimies of rotifers. Poultry dung was very effectively used to multiplicate rotifers. The fertilization ratio was about 8 kg each pond with 30cm depth of water. 2. The tiny rotifer, Filinia longiseta attained a very high population density of about 1,000 individuals per ml of pond water, but they were very sensitive to dipterex, and for this aspect future investigation may be needed. 3. In the outdoor ponds, the multiplication of rotifers significantly decreased when the water temperature falls to about $20^{\circ}C$ in autumn. 4. In the laboratory room, unicellular planktonic algae such as Scenedesmus or Chlorella, as the food of rotifers, were collected from the outdoor ponds by dipping them together with water, and were effectively used for the culture of Brachionus calyciflorus. If the planktonic algae are cultured in specially designed containers, the sun-light would be the most effective means as the source of light. 5. Brachionus calyciflorus cultured in the outdoor ponds by the dipterex controlled method was highly efficient to rear the early fry of marble gourami. The dipterex content mixed in the water to control the crustacean emmies of rotifers sieved no harm to the gourami fish fry.

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Investigation of Main Dishes on Literatures before the 17th Century (17세기 이전 주식류의 문헌적 고찰)

  • Kim, Eun-Mi;Cho, Shin-Ho;Chung, Rak-Won;Choi, Young-Jin;Won, Sun-Im;Cha, Gyung-Hee;Kim, Hyun-Sook;Lee, Hyo-Gee
    • Korean journal of food and cookery science
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    • v.22 no.3 s.93
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    • pp.314-336
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    • 2006
  • In this treatise, we investigated the Korean literature of main dishes before the 17th century : ${\ulcorner}Sangayorock{\lrcorner}$, ${\ulcorner}Sasichanyocho{\lrcorner}$, ${\ulcorner}Soowonjabbang{\lrcorner}$, ${\ulcorner}Yongjechongwha{\lrcorner}$, ${\ulcorner}Dongyoebogam{\lrcorner}$, ${\ulcorner}Domoondaejak{\lrcorner}$, ${\ulcorner}Geebongyouseul{\lrcorner}$, ${\ulcorner}New-Guwhangchalyo{\lrcorner}$, ${\ulcorner}Eumshickdimibang{\lrcorner}$,, ${\ulcorner}joobangmoon{\lrcorner}$, and ${\ulcorner}Yorock{\lrcorner}$. Main dishes were classified 19 kinds of rice, 64 kinds of gruel, 6 kinds of thin gruel, 1 kind of Moori, 1 kind of rice-cake soup, 37 kinds of noodles and 20 kinds of dumpling. Rice varieties were brown rice, rice, hulled rice, millet rice, foxtail millet rice, barley rice, sesame rice and flax seed rice. Gruel ingredients were cereal, beans, vegetables, nuts or seeds, medical plants or hardy wild plants, poultry, pork's kidney, dog's liver or oyster, clam and/or milk Rice-cake soup was 'Tangbyun' in ${\ulcorner}Domoondaejak{\lrcorner}$. Noodles were divided by main flours: wheat flour, buckwheat flour, mung bean flour and other flours. Noodles were made by pressing or cutting method. Pressing method used a mold with a hole. The husk of dumpling was made by wheat flour, buckwheat flour, mixed flour of wheat and buckwheat, sliced fish or meat, and/or a gray mullet. Another special method was 'Sangwha' made by fermentation. Bun stuffing of dumpling used fishes, meat, vegetables, beans, flours and seasonings. Cooking methods of dumpling were boiling, baking, steaming and pan-frying. From this investigation of the Korean main dishes described in the Korean literature before the 17th century, we expect to develop useful recipes for those who are concerned about health and want longevity, and thereby to advance the Korean food culture.