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http://dx.doi.org/10.7744/cnujas.2010.37.3.399

Identification of SNPs in Highly Variable Lysozyme Gene in Korean Native Chicken Populations  

Hoque, M.R. (Department of Animal Science and Biotechnology, Chungnam National University)
Kang, B.S. (Poultry Science Division, National Institute of Animal Science)
Lim, H.K. (The Graduate School of Bio & Information Technology, Hankyong National University)
Choi, K.D. (The Graduate School of Bio & Information Technology, Hankyong National University)
Lee, J.H. (Department of Animal Science and Biotechnology, Chungnam National University)
Publication Information
Korean Journal of Agricultural Science / v.37, no.3, 2010 , pp. 399-404 More about this Journal
Abstract
Single nucleotide polymorphisms (SNPs) in chicken lysozyme (LYZ) gene were investigated in this study. The identification of SNPs in both exon and intron in LYZ gene has led to understanding of evolution for the domestic chicken populations. A total of 24 samples from two Korean native commercial chicken populations (CCPs) were used for the initial identification of SNPs by mixing three DNA samples for sequencing experiments. By comparing with red jungle fowl (RJF), two commercial chicken populations have 18 common polymorphisms. Between two commercial chicken populations, 15 polymorphisms were identified. Of the 33 polymorphisms identified, two indels (21 and 4 bp) were found. Whereas, only one polymorphism in exon 2 at the bp position 1426 was a non-synonymous substitution (p.Ala49Val), indicating the amino acid changes. The identified non-synonymous substitution (p.Ala49Val) is located close to the catalytic sites of the enzyme, which might affect its activity. In our investigation, the polymorphisms in LYZ gene can provide broad ideas for the variation of Korean native chicken populations from the ancestor of chicken breeds as well as the some biological functions of the LYZ gene.
Keywords
Lysozyme gene; SNPs; Indel; Korean native chicken;
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