• Journal of Plant Biology
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• v.37 no.2
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• pp.167-173
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• 1994

Poly(A)+ RNA was selected from Canavalia lineata root nodule RNA through oligo(dT) cellulose column and used for construction of a cDNA library using λgt10-EcoRI arms. The size of the library was 7.2$\times$105 pfu/mL. A full length leghemoglobin (Lb) cDNA clone, pCILb1(687 bp) isolated with soybean Lb probe, contained one open reading frame (ORF) of 447 bp with 54 bp plus 186 bp at 5' and 3' untranslated region, respectively. A consensus sequence of plant translation start region (AAAATGGG) was found at 5' untranslated region, and two polyadenylation-related sequence (AATAAA, AATAAG) and a conserved motif between them (gACTTGTT) were found upstream of poly(A)+ tail consisted of 13 (A)s at 3' untranslated region. The ORF encoded a polypeptide consisted of 149 amino acids with a molecular weight of 16.2 kD. Deduced amino acid sequences showed high degree of homology values with those of other Lbs ranging from 66% (Casuarina glauca) to 85% (Glycine max).

• Journal of Industrial Technology
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• v.28 no.B
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• pp.53-57
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• 2008

From the extensive screening for small cryptic plasmid among about 23 lactic acid bacteria (LAB), 2.4 kb of cryptic plasmid was isolated from Lactobacillus farciminis strain KCTC 3681 and named as pLF24. The plasmid pLF24 was a circular molecule of 2,396 base-pairs in length with a G+C content of 38%. Two protein-coding sequences could be predicted. ORF1 and ORF2 showed homologies to plasmids of gram-positive bacteria. The replication protein coded by ORF2 and the plus origin, were similar to replication regions of other gram-positive bacteria as shown in plasmids such as pLH2, pLS141-1 and pLC2. The nucleotide sequence of pLF24 was deposited into Genbank data base with an accession number of EU429343. The newly isolated plasmid can be used for construction of shuttle vector in Lactobacillus bacteria.

• Journal of Korean Association for Spatial Structures
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• v.16 no.4
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• pp.133-140
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• 2016

SLIM AU(A plus U-shaped) composite beam had been developed for not only reducing the story height in residential and commercial building, but also saving the cost of floor construction. The structural performance and economic feasibility was sufficiently approved by means of structural experiments and analytical studies. Even though fire resistance of the SLIM AU composite beam was evaluated throughout furnace fire test, the fire performance of the composite beam using finite element analysis is not analysed yet. Therefore the predictions of fire resistance simulations with loading as well as temperature distribution of the composite beam are summarized in this paper.

• Journal of Korean Association for Spatial Structures
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• v.16 no.4
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• pp.53-58
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• 2016

SLIM AU(A plus U-shaped) composite beam was developed for reducing the story height in the residential buildings, and saving the cosrtuction cost of floor structures. Structural performance and economic feasibility of the composite beam have been sufficiently approved through the structural experiments and the analytical studies. However, the verification for fire safety is necessary for the practical application of the composite beam. The fire resistance tests with and without loading were performed for the fire safety verification, and the test results were summarized in this paper.

• Proceedings of the SAREK Conference
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• 2009.06a
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• pp.1412-1417
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• 2009

BIM(Building Information Modeling) is considered a new paradigm and a powerful method in building design, construction and maintenance. However, it is still hard to implement environmental performance analysis using BIM in design stages. This study aims to reveal practical problems and evaluate interoperability between building design information and performance analysis with case studies. Three case studies are performed using two environmental analysis programs (IES/VE, EnergyPlus) and BIM-based architectural design programs (Revit, GoogleSketchUp) in this paper.

• Journal of the Korean Mathematical Society
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• v.50 no.4
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• pp.865-877
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• 2013

The disjoint union of mapping class groups of surfaces forms a braided monoidal category $\mathcal{M}$, as the disjoint union of the braid groups $\mathcal{B}$ does. We give a concrete and geometric meaning of the braidings ${\beta}_{r,s}$ in $\mathcal{M}$. Moreover, we find a set of elements in the mapping class groups which correspond to the standard generators of the braid groups. Using this, we can define an obvious map ${\phi}\;:\;B_g{\rightarrow}{\Gamma}_{g,1}$. We show that this map ${\phi}$ is injective and nongeometric in the sense of Wajnryb. Since this map extends to a braided monoidal functor ${\Phi}\;:\;\mathcal{B}{\rightarrow}\mathcal{M}$, the integral homology homomorphism induced by ${\phi}$ is trivial in the stable range.

• Journal of the Korean Solar Energy Society
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• v.30 no.6
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• pp.1-9
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• 2010

Apartment balcony has been remodeled since the government permitted remodeling in January 2006.But extended balcony has great impact on building heat gain and loss. Therefore It has problems such as increase of heating and cooling energy. So $\underline{t}echnical$ solutions about window solar gain in summer is an urgent matter. The Purpose of this study is to evaluate energy performance of a blind in a double-skin facade in residential buildings by using EnergyPlus program. The results show that slat angles of $90^{\circ}$ is best in energy performance if we do not consider daylight. Poorly daylighted living room needs electric light and it also causes high cooling load. On the other hand, the results show that the application of blinds controlled automatically is best for energy performance when we consider daylight. Blind slat angles of $50\sim60^{\circ}$ have best performance when blinds are controlled in this angle throughout the day on a clear day in August. Blind slat angles of $0\sim30^{\circ}$ have best performance when blinds $\underline{are\;controlled}$ in this angle throughout the day on a cloudy day (more than 7 of total sky cover) in August.

• Journal of Microbiology and Biotechnology
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• v.27 no.9
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• pp.1586-1592
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• 2017

Some promoters were isolated and characterized from the genome of Leuconostoc mesenteroides SY2, an isolate from kimchi, a Korean traditional fermented vegetable. Chromosomal DNA of L. mesenteroides SY2 was digested with Sau3AI and ligated with BamHI-cut pBV5030, a promoter screening vector containing a promoterless cat-86. Among E. coli transformants (TFs) resistant against Cm (chloramphenicol), 17 were able to grow in the presence of $1,000{\mu}g/ml$ Cm and their inserts were sequenced. Transcription start sites were examined for three putative promoters (P04C, P25C, and P33C) by primer extension. Four putative promoters were inserted upstream of a promoterless ${\alpha}$-amylase reporter gene in $pJY15{\alpha}$. ${\alpha}$-Amylase activities of E. coli TFs containing $pJY15{\alpha}$ (control, no promoter), $pJY03{\alpha}$ ($pJY15{\alpha}$ with P03C), $pJY04{\alpha}$ (with P04C), $pJY25{\alpha}$ (with P25C), and $pJY33{\alpha}$ (with P33C) were 66.9, 78.7, 122.1, 70.8, and 99.3 U, respectively. Cells harboring $pJY04{\alpha}$ showed 1.8 times higher activity than the control. Some promoters characterized in this study might be useful for construction of food-grade expression vectors for Leuconostoc sp. and related lactic acid bacteria.

• Journal of Convergence for Information Technology
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• v.9 no.1
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• pp.54-60
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• 2019

The recent climate change is exacerbating the external thermal environment and increasing the amount of energy used in building. Energy Plus was used to evaluate low energy technology performance of buildings responding to climate change. The test types of basic building(control) and evaporative mist system + basic building(EMS), and the analysis results of each type are compared. Energy performance evaluation result, Cooling peak load were EMS reduction compared to control is about 9%. Annual cooling load per unit area were EMS reduction compared to control is about 17%. Annual energy use per unit area were EMS reduction compared to control is about 10%. Therefore, the effect of the evaporative cooling system is considered to be good through energy reduction technology of building, according to the amount and distance of the evaporative mist system in the future research on building energy performance evaluation should be carried out.

• Journal of the Architectural Institute of Korea Structure & Construction
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• v.36 no.5
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• pp.177-185
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• 2020

The building energy performance indicator, called Energy Performance Index (EPI), has been used for the past decades in South Korea. It has a list of design variables assigned with weighting factors (a, b). Unfortunately, the current EPI method is not performance-based but very close to a prescriptive rating. With this in mind, this study aims to propose a new performance-based EPI method. For this purpose, a global sensitivity analysis method, Sobol, is employed. The Sobol method is suitable for complex nonlinear models and can decompose all the output variance due to every input. The Sobol sensitivity index of each variable is defined as 0 to 1 (0 to 100%), and the sum of all sensitivity indices is equal to 1 (100%). In this study, an office building was modeled using EnergyPlus and then the Latin Hypercube Sampling (LHS) was conducted to generate a surrogate model to EnergyPlus. The sensitivity index was suggested to replace weight (a) in the existing EPI. In addition, the discrete weight (b) in the existing EPI was replaced by a set of continuous regression functions. Due to the introduction of the sensitivity index and the continuous regression functions, the new proposed approach can provide far more accurate outcome than the existing EPI (R²: 0.83 vs. R²: 0.01 for cooling, R²: 0.66 vs. R²: 0.01 for total energy). The new proposed approach proves to be more rational, objective and performance-based than the existing EPI method.