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Cultural Characteristics of Ectomycorrhizal Mushrooms

  • Jeon, Sung-Min;Ka, Kang-Hyeon
    • 한국균학회소식:학술대회논문집
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    • 2015.11a
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    • pp.16-16
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    • 2015
  • Ectomycorrhizal (ECM) mushrooms play a major role in plant growth promotion through symbiotic association with roots of forest trees. They also provide an economically important food resource to us and therefore they have been studied for their artificial cultivation for decades in Korea. We have secured bio-resources of ECM mushrooms from Korean forests and performed their physiological studies. To investigate the cultural characteristics, the fungi were cultured under different conditions (medium, temperature, pH of the medium, inorganic nitrogen source). More than 90% of total 160 strains grew on three solid media (potato dextrose agar, PDA; sabouraud dextrose agar, SDA; modified Melin-Norkrans medium, MMN). The rate of mycelial growth on malt extract agar (MEA) was lower than those of three media (PDA, SDA, MMN). None of the Tricholomataceae strains grew on MEA. Many strains of ECM mushrooms were able to grow at the temperature range of $15{\sim}25^{\circ}C$ on PDA, while they showed poor growth at $10^{\circ}C$ or $30^{\circ}C$. In particular, the growth rates of both Gomphaceae and Tricholomataceae were significantly lower at $10^{\circ}C$ than at $30^{\circ}C$. The optimal pH of many strains was pH 5.0 when they cultured in potato dextrose broth (PDB). Fifty-seven percent of tested strains grew well on medium containing ammonium source than nitrate source. Many strains of Tricholomataceae showed a notable growth on ammonium medium than nitrate medium. Twenty-three percent of strains preferred nitrate source than ammonium source for their mycelial growth. The production and activity of two enzymes (cellulase and laccase) by ECM fungi were also assayed on the enzyme screening media containing CMC or ABTS. Each strains exhibited different levels of enzymatic activities as well as enzyme production. The number of laccase-producing strains was less than that of cellulase-producing strains. We found that 77% of tested strains produced both cellulase and laccase, whereas 2% of strains did not produce any enzymes. The morphological characteristics of mycelial colony were also examined on four different solid media. Yellow was a dominant color in mycelial colony and followed by white and brown on all culture media. ECM mushrooms formed mycelial colonies with a single or multiple colors within a culture medium depending on the strains and culture media. The most common shape of mycelial colony was a circular form on all media tested. Other families except for Amanitaceae formed an irregular colony on MMN than PDA. All strains of Tricholomataceae did not form a filamentous colony on all media. The pigmentation of culture media by mycelial colonies was observed in more than 50% of strains tested on both PDA and SDA. The degree of pigmentation on PDA or SDA was higher than MMN and brown color was dominant than yellow color. The production of exudates from mycelial colony was higher on PDA than MMN. Brown exudates were mainly produced by many strains on PDA or SDA, whereas transparent exudates were mainly produced by strains on MMN. We observed the mycelial colonies with a single or multiple textures in just one culture plate. Wrinkled or uneven colony surfaces were remarkably observed in many strains on PDA or SDA, while an even colony surface was observed in many strains on MMN. Sixty percent of Tricholomaceae strains formed wrinkled surface on PDA. However, they did not form any wrinkle on MMN plate. Cottony texture was observed in mycelia colonies of many strains. Velvety texture was often observed in the mycelial colonies on SDA than PDA and accounted for 60% of Suillaceae strains on SDA.

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Microbial quality of fresh vegetables in restaurants around school (학교주변식당 신선 채소류의 미생물 오염도 조사)

  • Jo, So Hyun;Chung, Hyun-Jung;Lee, Seong Hee;Hwang, Su Jung;Om, Ae-Son;Eun, Jong-Bang
    • Food Science and Preservation
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    • v.20 no.3
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    • pp.424-428
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    • 2013
  • Microbiological contamination of 4 vegetables (garlic, red pepper, perilla leaf and lettuce) collected from 10 restaurants around university was examined. The vegetables were evaluated for total plate count, coliforms, psychrophiles, yeast, and Staphylococcus aureus. The results of total plate count showed the highest value as $5.4{\pm}0.69$ log CFU/g in lettuce, following by $4.8{\pm}1.53$ log CFU/g in red pepper, $4.5{\pm}1.65$ log CFU/g in perilla leaf and $3.4{\pm}1.27$ log CFU/g in garlic. The contamination level of coliforms and psychrophiles were highest in red pepper with maximum as 4.7 log CFU/g and 8.2 log CFU/g, respectively. Red pepper of psychrophiles showed the highest average value as $5.0{\pm}1.82$ log CFU/g followed by $4.2{\pm}1.91$ log CFU/g in lettuce, $4.7{\pm}1.55$ log CFU/g in perilla leaf and $2.4{\pm}2.10$ log CFU/g in garlic. The average number of yeasts were highest in perilla leaf with $4.4{\pm}1.41$ log CFU/g and were lowest in garlic with $0.9{\pm}1.41$ log CFU/g. The contamination level of S. aureus was detected in 27 samples among the total 40 samples with the range of 0.5-5.2 log CFU/g. In conclusion, the microbial quality of the fresh vegetables evaluated in this study was not very good. Therefore, it needs to be enhanced through the good sanitation management and production and distribution methods to improve the safety of fresh vegetables.

Recent Progress in Air-Conditioning and Refrigeration Research: A Review of Papers Published in the Korean Journal of Air-Conditioning and Refrigeration Engineering in 2014 (설비공학 분야의 최근 연구 동향: 2014년 학회지 논문에 대한 종합적 고찰)

  • Lee, Dae-Young;Kim, Sa Ryang;Kim, Hyun-Jung;Kim, Dong-Seon;Park, Jun-Seok;Ihm, Pyeong Chan
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.27 no.7
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    • pp.380-394
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    • 2015
  • This article reviews the papers published in the Korean Journal of Air-Conditioning and Refrigeration Engineering during 2014. It is intended to understand the status of current research in the areas of heating, cooling, ventilation, sanitation, and indoor environments of buildings and plant facilities. Conclusions are as follows. (1) The research works on the thermal and fluid engineering have been reviewed as groups of heat and mass transfer, cooling and heating, and air-conditioning, the flow inside building rooms, and smoke control on fire. Research issues dealing with duct and pipe were reduced, but flows inside building rooms, and smoke controls were newly added in thermal and fluid engineering research area. (2) Research works on heat transfer area have been reviewed in the categories of heat transfer characteristics, pool boiling and condensing heat transfer and industrial heat exchangers. Researches on heat transfer characteristics included the results for thermal contact resistance measurement of metal interface, a fan coil with an oval-type heat exchanger, fouling characteristics of plate heat exchangers, effect of rib pitch in a two wall divergent channel, semi-empirical analysis in vertical mesoscale tubes, an integrated drying machine, microscale surface wrinkles, brazed plate heat exchangers, numerical analysis in printed circuit heat exchanger. In the area of pool boiling and condensing, non-uniform air flow, PCM applied thermal storage wall system, a new wavy cylindrical shape capsule, and HFC32/HFC152a mixtures on enhanced tubes, were actively studied. In the area of industrial heat exchangers, researches on solar water storage tank, effective design on the inserting part of refrigerator door gasket, impact of different boundary conditions in generating g-function, various construction of SCW type ground heat exchanger and a heat pump for closed cooling water heat recovery were performed. (3) In the field of refrigeration, various studies were carried out in the categories of refrigeration cycle, alternative refrigeration and modelling and controls including energy recoveries from industrial boilers and vehicles, improvement of dehumidification systems, novel defrost systems, fault diagnosis and optimum controls for heat pump systems. It is particularly notable that a substantial number of studies were dedicated for the development of air-conditioning and power recovery systems for electric vehicles in this year. (4) In building mechanical system research fields, seventeen studies were reported for achieving effective design of the mechanical systems, and also for maximizing the energy efficiency of buildings. The topics of the studies included energy performance, HVAC system, ventilation, and renewable energies, piping in the buildings. Proposed designs, performance performance tests using numerical methods and experiments provide useful information and key data which can improve the energy efficiency of the buildings. (5) The field of architectural environment was mostly focused on indoor environment and building energy. The main researches of indoor environment were related to the evaluation of work noise in tunnel construction and the simulation and development of a light-shelf system. The subjects of building energy were worked on the energy saving of office building applied with window blind and phase change material(PCM), a method of existing building energy simulation using energy audit data, the estimation of thermal consumption unit of apartment building and its case studies, dynamic window performance, a writing method of energy consumption report and energy estimation of apartment building using district heating system. The remained studies were related to the improvement of architectural engineering education system for plant engineering industry, estimating cooling and heating degree days for variable base temperature, a prediction method of underground temperature, the comfort control algorithm of car air conditioner, the smoke control performance evaluation of high-rise building, evaluation of thermal energy systems of bio safety laboratory and a development of measuring device of solar heat gain coefficient of fenestration system.

An Experimental Study on the Seismic Performance of Shear Connections and Rib Plate H Beam to Column Connections (전단접합 및 리브 플레이트로 보강한 H형 보-기둥 접합부의 내진성능에 관한 실험적 연구)

  • Oh, Kyung Hyun;Seo, Seong Yeon;Kim, Sung Yong;Yang, Young Sung;Kim, Kyu Suk
    • Journal of Korean Society of Steel Construction
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    • v.17 no.5 s.78
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    • pp.569-580
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    • 2005
  • The postbeam joint connection of the existing steel structure moment flexible frame system did not produce sufficient seismic resistance during the earthquakes in Northridge and Kobe, and it sustained brittle fracturing on the joint connection. This study was performed to execute the high-tensile bolt share connection of H-beams web and the full-scale experiment as a parameter of the existing reinforcement of H-flange rib, by making the shape of the existing joint connection. This experiment was performed to determine the extent of the decrease of the number of high-tensile bolts and how to improve workability of the two-phase shear connection of web beam. In addition, this study was performed to enhance the seismic resistant capacity through the enforcement of rib plates. As a result of the experiment of two-phase shear connection of H-beam web and of joint connection to be reinforced by rib plates, the results of this study showed that the initial stiffness, energy-dissipation capacity, and rotational capacity of plasticity was higher than the existing joint connection. As to the rate of increasing the strength and deformation capacity, there were differences between the tension side and compression side because of the position of shear tap. However, as a whole, they have shown excellent seismic resistant capacity. Also, all the test subjects exceeded 4% (rate of delamination), about 0.029 rad (total plastic capacity), and about 130% (maximum strength of joint connection) of fully plastic moment for the original section. Accordingly, this study was considered as it would be available in the design more than the intermediate-level of moment flexible frame.

Axial Collapse Behaviour of Ship's Stiffened Panels considering Lateral Pressure Load (횡하중을 고려한 선체보강판넬의 압축 붕괴거동에 관한 연구)

  • Ko, Jae-Yong;Park, Joo-Shin
    • Journal of Navigation and Port Research
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    • v.31 no.3 s.119
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    • pp.235-245
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    • 2007
  • Stiffened steel plates are basic structural members on the deck and bottom structure in ship, offshore. It has a number of one sided stiffeners in either one or both directions, the latter structure was called grillage structure. At the ship structural desgn stage, one of the major consideration is evaluation for ultimate strength of the hull girder. In general, it is accepted that hull girder strength can be represented by the local strength of the longitudinal stiffened panel. In case of considering hogging condition in a stormy sea, stiffened panel was acting on the bottom structure under axial compressive load induced hull girder bending moment, also simultaneously arising local bending moment induced lateral pressure load. In this paper, results of the structural analysis have been compared with another detailed FEA program and prediction from design guideline and a series analysis was conducted consideration of changing parameters for instance, analysis range, cross-section of stiffener, web height and amplitude of lateral pressure load subjected to combined load (axial compression and lateral pressure load). It has been found that finite element modeling is capable of predicting the behaviour and ultimate load capacity of a simply supported stiffened plate subjected to combined load of axial compression and lateral pressure load It is expected that these results will be used to examine the effect of interaction between lateral pressure and axial loads for the ultimate load-carrying capacity based on the Ultimate Limit State design guideline.

Analysis of Attached Bacterial Communities of Biological Activated Carbon Process Using DGGE Method (DGGE 기법을 이용한 생물활성탄 공정의 부착 박테리아 군집분석)

  • Son, Hee-Jong;Choi, Jin-Taek;Son, Hyeng-Sik;Lee, Sang-Joon
    • Journal of Korean Society of Environmental Engineers
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    • v.34 no.8
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    • pp.533-540
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    • 2012
  • The concentration of organic compounds was analyzed at each step of BAC (biological activated carbon) process though BDOC (biodegradable dissolved organic carbon) total/rapid/slow. Further, bacteria communities and biomass concentrations measured DGGE (denaturing gradirnt gel electrophoresis) and ATP (adenosine triphosphate) methods were analyzed. The bed volume of steady state is different based on assessment of organic compounds removal. Bed volumes at steady state in DOC, $BDOC_{rapid}$ and $BDOC_{total/slow}$ removal were around 27,500, 15,000 and 32,000, respectively. A biomass didn't change after the bed volume reached 22,500 according to analyzing HPC (heterotrophic plate count) and ATP concentration of bacteria. The concentration of HPC and ATP were $3.3{\times}10^8$ cells/g and $2.14{\mu}g/g$, respectively. The number of the DGGE band were only 5 at the bed volume 8,916, but increased up to 11 at the bed volume 49,632. As operation time increase, bacterial group were more diversity. Four bacteria species including Pseudomonas fluorescens, the uncultured bacterium similar to Acinetobacteria, uncultured Novosphingobium sp. and Flavobacterium frigidarium have detected from the early stages and Proteobacteria group were dominantly detected.

Numerical Analysis of Natural Convection in Inclined Flat Plate Enclosures (경사진 평판형 밀폐 공간에서의 자연 대류 현상의 수치 해석)

  • Kim, Yong Hyun;Koh, Hak Kyun;Noh, Sang Ha
    • Journal of Biosystems Engineering
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    • v.10 no.1
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    • pp.24-38
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    • 1985
  • 경사진 밀폐 공간에서 마주 보는 두 벽면의 온도 차로 인하여 발생되는 자연 대류 현상은 여러 공학 분야에서 볼 수 있는 중요한 열전달 현상으로서, 최근 들어 평판형 태양열 집열기를 설계하려는 사람들에게 많은 관심의 대상이 되고 있다. 평판형 태양열 집열기의 경우 덮개판으로 부터의 대류 열손실을 감소시킴으로서 집열 효율을 높일 수 있을 뿐만 아니라 사용목적에 따라 소형 집열기를 제작할 수 있어 경제적으로 유리하게 될 것이다. 밀폐된 공간에서 최초에 정지 상태에 있는 얇은 유체층을 하부에서 가열시켜 주면 열팽창 현상이 일어나고, 이것에 의한 부력이 점도나 열전도도 등의 안정화 요인을 극복할 수 있을 정도로 커지면 System이 불안정하게 되어 자연 대류 현상이 수반되며 이 때문에 열전달율이 급격히 증가하게 된다. 이러한 현상의 지배 방정식은 연립 비선형 편미분 방정식으로 특수한 경계 조건외에는 일반적으로 해석적 해를 구하기가 어렵기 때문에 실험적 연구가 많이 실시되어 왔고 이들 결과의 대부분은 전반적인 열전달 특성치만을 구하는데 집중되어 왔다. 본 연구에서는 수치 해석법의 하나인 유한 차분법을 도입하여 이차원으로 가정한 경사진 평판형 밀폐 공간에서의 자연 대류 현상의 지배 방정식을 유한 차분화시켜, $$2.74{\times}10^3\leq_-Gr\leq_-2.0{\times}10^6$$, Pr=0.73, $$15^{\circ}\leq_-a\leq_-150^{\circ}$$, 종횡비는 1, 2, 3, 5, 9에 대하여 정상 상태에서의 해를 구하면서 수치적으로 실험하였다. 본 연구에서 얻어진 결론을 요약하면 다음과 같다. (1) 해석적으로 구하기 어려운 경사진 밀폐 공간에서 자연대류현상의 지배 방정식을 유한 차분법으로 해결할 수 있으며, 대류항과 확산항을 따로 고려한 유한차분법이 효과적임을 확인하였다. (2) 저온과 고온 벽면에서의 온도를 각각 균일하게 놓고 단변으로 이루어진 벽면은 완전히 절연되어 있는 경우에 대하여 수치해를 구한결과, 이전의 해석적 및 실험적 결과와 일치하였으며, 시간의 경과에 따른 온도 및 유선의 변화를 현상학적으로 관찰할 수 있었다. (3) 평균 열전달 계수에 미치는 경사각의 효과를 살펴본 결과 종횡비가 1 인 경우 경사각이 $45^{\circ}$에서, 종횡비가 2, 3, 5, 9인 경우 경사각이 $60^{\circ}$에서 각각 평균 열전달 계수 최대치가 나타났다. (4) Ra수(Rayleigh number) 가 증가될수록, 경사각에 상관없이 평균 열전달 계수도 증가되었다. 한편 Ra수 및 경사각의 변화에 따라 종횡비가 증가될수록 평균 열전달 계수는 경사각이 $90^{\circ}$인 경우를 제외하고는 감소됨을 볼 수 있었다. 경사각이 $90^{\circ}$인 경우, 평균 열전달 계수는 종횡비가 2 인 곳에서 최대치를 얻을 수 있었으며, 종횡비가 계속 증가될수록 평균 열전달 계수는 점차 감소되어짐을 볼 수 있었다.

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Mixed Mode Analysis using Two-step Extension Based VCCT in an Inclined Center Crack Repaired by Composite Patching (복합재료 팻칭에 의한 중앙경사균열에서 2단계 확장 가상균열닫힘법을 사용한 혼합모우드해석)

  • Ahn, Jae-Seok;Woo, Kwang-Sung
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.32 no.1A
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    • pp.11-18
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    • 2012
  • This paper deals with the numerical determination of the stress intensity factors of cracked aluminum plates under the mixed mode of $K_I$ and $K_{II}$ in glass-epoxy fiber reinforced composites. For the stress intensity factors, two different models are reviewed such as VCCT and two-step extension method. The p-convergent partial layerwise model is adopted to determine the fracture parameters in terms of energy release rates and stress intensity factors. The p-convergent approach is based on the concept of subparametric element. In assumed displacement field, strain-displacement relations and 3-D constitutive equations of a layer are obtained by combination of 2-D and 1-D higher-order shape functions. In the elements, Lobatto shape functions and Gauss-Lobatto technique are employed to interpolate displacement fields and to implement numerical quadrature. Using the models and techniques considered, effects of composite laminate configuration according to inclined angles and adhesive properties on the performance of bonded composite patch are investigated. In addition to these, the out-of-plane bending effect has been investigated across the thickness of patch repaired laminate plates due to the change of neutral axis. The present model provides accuracy and simplicity in terms of stress intensity factors, stress distribution, number of degrees of freedom, and energy release rates as compared with previous works in literatures.

Application of nonlocal elasticity theory for buckling analysis of nano-scale plates (나노 스케일 판의 좌굴해석을 위한 비국소 탄성 이론의 적용)

  • Lee, Won-Hong;Han, Sung-Cheon;Park, Weon-Tae
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.13 no.11
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    • pp.5542-5550
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    • 2012
  • Third-order shear deformation theory is reformulated using the nonlocal elasticity of Eringen. The equation of equilibrium of the nonlocal elasticity are derived. This theory has ability to capture the both small scale effects and quadratic variation of shear strain through the plate thickness. Navier's method has been used to solve the governing equations for all edges simply supported boundary conditions. Analytical solutions of buckling of nano-scale plates are presented using this theory to illustrate the effect of nonlocal theory on buckling load of the nano-scale plates. The relations between nonlocal third-order and local theories are discussed by numerical results. Further, effects of (i) length (ii) nonlocal parameter, (iii) aspect ratio and (iv) mode number on nondimensional buckling load are studied. In order to validate the present solutions, the reference solutions are used and discussed. The present results of nano-scale plates using the nonlocal theory can provide a useful benchmark to check the accuracy of related numerical solutions.

Improvement Effect and Field Application of Dynamic Replacement Using Crushed Rock (암버력 매립층의 동치환공법 현장 적용성 및 개량효과에 관한 연구)

  • Lee, In-Hwan;Lee, Chul-Hee;Shin, Eun Chul
    • Journal of the Korean Geosynthetics Society
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    • v.18 no.4
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    • pp.1-13
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    • 2019
  • The purpose of this study is to examine the effect of soft ground improvement by dynamic replacement with utilizing crushed rock. In order to understand the ground improvement effect when applying dynamic replacement method with crushed rock, the laboratory test and field test were performed. The internal friction angle and apparent cohesion were derived through direct shear test. The dynamic replacement characteristics were identified by analyzing the weight, drop, and number of blows needed for dynamic replacement. Through the field plate bearing test and density test, the bearing capacity and settlement of the improved ground were measured, and the numerical analysis were conducted to analyze the behavior of the improved ground. In this study, it proposes modified soil experimental coefficient(CDR) to 0.3~0.5 in the dynamic replacement method with crushed rock. Also when applying the dynamic replacement method using crushed rock, the particle size range is less than 100 mm, D90 is less than 80 mm and D15 is more than 30 mm.