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http://dx.doi.org/10.4491/KSEE.2012.34.8.533

Analysis of Attached Bacterial Communities of Biological Activated Carbon Process Using DGGE Method  

Son, Hee-Jong (Busan Water Quality Institute, Water Authority)
Choi, Jin-Taek (Busan Water Quality Institute, Water Authority)
Son, Hyeng-Sik (Department of Microbiology, Pusan National University)
Lee, Sang-Joon (Department of Microbiology, Pusan National University)
Publication Information
Journal of Korean Society of Environmental Engineers / v.34, no.8, 2012 , pp. 533-540 More about this Journal
Abstract
The concentration of organic compounds was analyzed at each step of BAC (biological activated carbon) process though BDOC (biodegradable dissolved organic carbon) total/rapid/slow. Further, bacteria communities and biomass concentrations measured DGGE (denaturing gradirnt gel electrophoresis) and ATP (adenosine triphosphate) methods were analyzed. The bed volume of steady state is different based on assessment of organic compounds removal. Bed volumes at steady state in DOC, $BDOC_{rapid}$ and $BDOC_{total/slow}$ removal were around 27,500, 15,000 and 32,000, respectively. A biomass didn't change after the bed volume reached 22,500 according to analyzing HPC (heterotrophic plate count) and ATP concentration of bacteria. The concentration of HPC and ATP were $3.3{\times}10^8$ cells/g and $2.14{\mu}g/g$, respectively. The number of the DGGE band were only 5 at the bed volume 8,916, but increased up to 11 at the bed volume 49,632. As operation time increase, bacterial group were more diversity. Four bacteria species including Pseudomonas fluorescens, the uncultured bacterium similar to Acinetobacteria, uncultured Novosphingobium sp. and Flavobacterium frigidarium have detected from the early stages and Proteobacteria group were dominantly detected.
Keywords
Biological Activated Carbon (BAC); Attached Bacterial Community; Denaturing Gradirnt Gel Electrophoresis (DGGE); Biomass; Adenosine Triphosphate (ATP); Biodegradable Dissolve Organic Carbon (BDOC); Bed Volume;
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Times Cited By KSCI : 1  (Citation Analysis)
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