• Title/Summary/Keyword: plant transfer

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Efficient Plantlet Regeneration via Callus Formation from Leaf Segment of Lilium Oriental Hybrid 'Casa Blanca'

  • Kim Mi-Sun;Jeon Jae-Heung;Youm Jung-Won;Kim Jae-Hyun;Lee Byung-Chan;Kang Won-Jin;Kim Hyun-Soon;Joung Hyouk
    • Journal of Plant Biotechnology
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    • v.7 no.2
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    • pp.129-134
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    • 2005
  • Callus induction from a leaf explant has been achieved in Lilium Oriental hybrid 'Casa Blanca'. The highest frequency of callus induction was obtained on MS medium supplemented with 0.5 mg/L BA and 2.0 mg/L NAA after 2 months of culture. The cultures maintained continuously without change in color and type of callus when they cultured in the dark. Plantlet regeneration with a high frequency was achieved from induced calli on the same medium. A number of shoots are formed from one cluster of callus, and bulblets developed into intact plantlets after transfer to hormone-free MS medium. No phenotypic variations were observed among regenerants. Enhancement in plantlet regeneration via callus formation would be expected to facilitate the efficiency of transformation of this Oriental hybrid 'Casa Blanca'.

Methods for Introduction of the Atmospheric Nitrogen Fixing Ability to Plants

  • PreiningerE;BokaK;ZatykoJ;KoranyiP;GyurjanI
    • Journal of Plant Biotechnology
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    • v.1 no.1
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    • pp.31-38
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    • 1999
  • An artificial symbiosis was established between diazotropic Azomonas insignis and strawberry (Fragaria x ananassa). The partnership was created by in vitro techniques through callus induction and organogenesis. The basis of this partnerships is the bacterial dependence on the plants metabolic activity, using maltose in the medium as a carbon and energy source which can be utilized by the plant cells only. The presence of bacteria in the intercellular spaces of the callus tissues and regenerated plants was proven by microscopic techniques. Nitrogenase activity could also be detected in the plant tissues. For successful and high frequency introduction of bacteria to the plant tissues, biolistic gun method was used. On the basis of the DNA transfer method, Azotobacter vinelandii bacteria were delivered directly into strawberry tissues by the particle bombardment. This was the first use of living bacteria as microprojectils for bombardment of plant tissues. The treatment was successful, the presence of bacteria in the developing callus tissue and regenerated plants were detected by light and electron microscopy.

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A Gain-Phase Loop Shaping Method of QFT using TLS (TLS를 이용한 QFT의 이득-위상 루프형성법)

  • Kim, Ju-Sik;Jeong, Su-Hyun
    • The Transactions of the Korean Institute of Electrical Engineers P
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    • v.51 no.2
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    • pp.94-98
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    • 2002
  • QFT(Quantitative Feedback Theory) is a very practical design technique that emphasizes the use of feedback for achieving the desired system performance tolerances in despite of plant uncertainty and disturbance. The gain-phase loop shaping procedure of QFT is employed to design controller, until the bounds at desired frequencies are satisfied. This paper presents a transfer function synthesis using TLS(Total Least Squares) and offers a loop shaping method with the suggested technique. An example illustrates a feasibility of the presented algorithm.

Gradational Risk Management in Procurement Phase for Plant Projects (플랜트 프로젝트의 구매조달 단계별 리스크 분석에 관한 연구)

  • Na, Sung-Yub;Moon, Seung-Jae;Yoo, Hoseon
    • Plant Journal
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    • v.5 no.3
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    • pp.59-65
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    • 2009
  • In the plant EPC project, the procurement work can be considered as the most important part that affects the success or failure of project as it has the closest mutual influence in every execution process of the project. Procurement takes up generally 60% ~ 70% of the entire project budget and the current trend of plant EPC project becoming larger in scale and a variety of process technology being applied. So, it is needed to identify the risks that are expected to occur according to the stages of procurement work, namely, proposal stage, manufacturer selection and purchasing agreement stage, expediting the process management of manufacturer stage, inspection stage and logistics & transportation stage. The management of observation and control of the risks should be performed with the response techniques which are 'avoid',' transfer'and' mitigate'.

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Somatic Embryogenesis and Plant Regeneration in Tissue Cultures of Artemisia annua L.

  • Choi, Pil-Son;Min, Sung-Ran;Ko, Suk-Min;Liu, Jang-R.
    • Journal of Plant Biotechnology
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    • v.34 no.3
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    • pp.197-200
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    • 2007
  • Mature seeds of Artemisia annua L. were placed onto Murashige and Skoog's (MS) medium supplemented with $4.52\;{\mu}M$ 2,4-dichlorophenoxyacetic acid (2,4-D). After 6 weeks of culture, off-white, compact calluses were formed on the plumule of seedlings at a frequency of 5.9%. Calluses were subcultured on the same medium. After an additional 2 weeks of subculture, calluses produced a few somatic embryos at a frequency of 28.8%. Upon transfer to MS basal medium, calluses producing a few somatic embryos gave rise to numerous somatic embryos, which subsequently developed into plantlets. Plantlets were successfully transplanted to potting soil and grown to maturity in a greenhouse.

A Study on Start·Stop System at Water Turbine-Generator for Tidal Power Plant (조력발전용 수차발전기의 기동·정지시스템에 관한 연구)

  • Oh, Min-Hwan;Park, Chul-Won
    • The Transactions of the Korean Institute of Electrical Engineers P
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    • v.63 no.2
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    • pp.113-118
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    • 2014
  • Tidal power is one of new and renewable energy sources. Tidal power is generated by using the gap in the water level between the water outside and inside the embankment. All tidal power plant in Korea were being operated by import of turn-key from abroad. The know-how and technology which are the most important to build predictive control system has become increasingly difficult to obtain from advanced countries because most of them avoid to transfer, which the domestic development of the control system is needed. In this paper, a study on start stop system at water turbine-generator for tidal power plant at the beginning of development was presented. For improvement the efficiency and develope of core technology of the start stop system, the technique and characteristics of tidal power, modeling, maximum generation calculation method, and optimal control of joint control system in Sihwa tidal power plant were studied.

Determining thermal comfort properties of coverall worn in the atomic power plant using a sweating thermal manikin and ISO 7730 (발한 Thermal manikin과 국제 표준 7730을 이용한 원자력 발전소 작업복의 열적 쾌적성 판별)

  • 홍성애
    • Journal of the Ergonomics Society of Korea
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    • v.15 no.1
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    • pp.91-103
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    • 1996
  • For determining thermal comfort properties of work suit in an atomic power plant, three different coverall ensembles (PVE, PET/Rayon, PP Nonwoven) were selected and the resistance to dry and evaporative heat transfer were measured for each ensemble by using a sweating thermal manikin. Also, PMV (Predicted Mean Vote) and PPD(Predicted Percentage of Dissatisfied) indices were predicted according to ISO 7730. As a result, ideal environmental conditions in an atomic power plant were suggested to make workers feel thermally comfortable. In addition, ideal intrinsic insulation values of coverall ensembles as a work suit under the present environmental conditions in the at6omic power plant were provided. The information given in this paper can be used to control environmental conditions in the atomic power plant thermally comfortable and to select a proper work suit for providing thermal comfort to the workers.

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Heat transfer enhancement of nanofluids in a pulsating heat pipe for heat dissipation of LED lighting

  • Kim, Hyoung-Tak;Bang, Kwang-Hyun
    • Journal of Advanced Marine Engineering and Technology
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    • v.38 no.10
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    • pp.1200-1205
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    • 2014
  • The effect of nanofluids on the heat transfer performance of a pulsating heat pipe has been experimentally investigated. Water-based diamond nanofluid and aluminium oxide ($Al_2O_3$) nanofluid were tested in the concentration range of 0.5-5%. The pulsating heat pipe was constructed using clear Pyrex tubes of 1.85 mm in inner diameter in order to visualize the pulsating action. The total number of turns was eight each for heated and cooled parts. The supply temperatures of heating water and cooling water were fixed at $80^{\circ}C$ and $25^{\circ}C$ respectively. The liquid charging ratio of the nanofluid was 50-70%. The test results showed that the case of 5% concentration of diamond nanofluid showed 18% increase in heat transfer rate compared to pure water. The case of 0.5% concentration of $Al_2O_3$ nanofluid showed 24% increase in heat transfer rate compared to pure water. But the increase of $Al_2O_3$ nanofluid concentration up to 3% did not show further enhancement in heat transfer. It is also observed that the deposited nanoparticles on the tube wall played a major role in enhanced evaporation of working fluid and this could be the reason for the enhancement of heat transfer by a nanofluid, not the enhanced thermal conductivity of the nanofluid.

Effects of Condensation Heat Transfer Model in Calculation for KNGR Containment Pressure and Temperature Response

  • Eoh, Jae-Hyuk;Park, Shane;Jeun, Gyoo-Dong;Kim, Moo-Hwan
    • Nuclear Engineering and Technology
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    • v.33 no.2
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    • pp.241-253
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    • 2001
  • Under severe accidents, the pressure and temperature response has an important role for the integrity of a nuclear power plant containment. The history of the pressure and temperature is characterized by the amount and state of steam/air mixture in a containment. Recently, the heat transfer rate to the structure surface is supposed to be increased by the wavy interface formed on condensate film. However, in the calculation by using CONTAIN code, the condensation heat transfer on a containment wall is calculated by assuming the smooth interface and has a tendency to be underestimated for safety. In order to obtain the best- estimate heat transfer calculation, we investigated the condensation heat transfer model in CONTAIN 1.2 code and adopted the new forced convection correlation which is considering wavy interface. By using the film tracking model in CONTAIN 1.2 code, the condensate film is treated to consider the effect of wavy interface. And also, it was carried out to investigate the effect of the different cell modelings - 5-cell and 10-cell modeling - for KNGR(Korean Next Generation Reactor) containment phenomena during a severe accident. The effect of wavy interface on condensate film appears to cause the decrease of peak temperature and pressure response . In order to obtain more adequate results, the proper cell modeling was required to consider the proper flow of steam/air mixture.

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