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http://dx.doi.org/10.5010/JPB.2007.34.3.197

Somatic Embryogenesis and Plant Regeneration in Tissue Cultures of Artemisia annua L.  

Choi, Pil-Son (Eugentech Inc., Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Min, Sung-Ran (Plant Genome Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Ko, Suk-Min (Eugentech Inc., Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Liu, Jang-R. (Plant Genome Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Publication Information
Journal of Plant Biotechnology / v.34, no.3, 2007 , pp. 197-200 More about this Journal
Abstract
Mature seeds of Artemisia annua L. were placed onto Murashige and Skoog's (MS) medium supplemented with $4.52\;{\mu}M$ 2,4-dichlorophenoxyacetic acid (2,4-D). After 6 weeks of culture, off-white, compact calluses were formed on the plumule of seedlings at a frequency of 5.9%. Calluses were subcultured on the same medium. After an additional 2 weeks of subculture, calluses produced a few somatic embryos at a frequency of 28.8%. Upon transfer to MS basal medium, calluses producing a few somatic embryos gave rise to numerous somatic embryos, which subsequently developed into plantlets. Plantlets were successfully transplanted to potting soil and grown to maturity in a greenhouse.
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