Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Efficient Plantlet Regeneration via Callus Formation from Leaf Segment of Lilium Oriental Hybrid 'Casa Blanca'

  • Kim Mi-Sun (Plant Cell Biotechnology Lab., Korea Research Institute of Bioscience and Biotechnology) ;
  • Jeon Jae-Heung (Plant Cell Biotechnology Lab., Korea Research Institute of Bioscience and Biotechnology) ;
  • Youm Jung-Won (Plant Cell Biotechnology Lab., Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim Jae-Hyun (Plant Cell Biotechnology Lab., Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee Byung-Chan (Plant Cell Biotechnology Lab., Korea Research Institute of Bioscience and Biotechnology) ;
  • Kang Won-Jin (Plant Cell Biotechnology Lab., Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim Hyun-Soon (Plant Cell Biotechnology Lab., Korea Research Institute of Bioscience and Biotechnology) ;
  • Joung Hyouk (Plant Cell Biotechnology Lab., Korea Research Institute of Bioscience and Biotechnology)
  • Published : 2005.06.01
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Abstract

Callus induction from a leaf explant has been achieved in Lilium Oriental hybrid 'Casa Blanca'. The highest frequency of callus induction was obtained on MS medium supplemented with 0.5 mg/L BA and 2.0 mg/L NAA after 2 months of culture. The cultures maintained continuously without change in color and type of callus when they cultured in the dark. Plantlet regeneration with a high frequency was achieved from induced calli on the same medium. A number of shoots are formed from one cluster of callus, and bulblets developed into intact plantlets after transfer to hormone-free MS medium. No phenotypic variations were observed among regenerants. Enhancement in plantlet regeneration via callus formation would be expected to facilitate the efficiency of transformation of this Oriental hybrid 'Casa Blanca'.

Keywords

References

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