• 대한본초학회지
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• 제22권2호
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• pp.103-108
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• 2007

Objectives : A morphological classification among Pinelliae Tuber, Typhonii Flagelliformis Rhizoma and Arisaematis Rhizoma was made through microscopic observation. Method : The slice of the tested material made by paraffin section technique was colored with Safranine Malachite Green contrast methods, and then observed and photographed by olymphus-BHT. Result : 1. Pinelliae Tuber is like a ball with a $1{\sim}1.5cm$ diameter, with a concave apex and pockmarked root trace around. 2. Typhonii Flagelliformis Rhizoma is somewhat oval or like a cone, with a $4{\sim}8mm$ diameter, a $7{\sim}15mm$ height, a projected round apex, and a pin-pointed back. 3. Arisaematis Rhizoma is somewhat flat round with a $2{\sim}2.5cm$ diameter, a little than Pinelliae Tuber, and its apex has incomplete lobopodium and root trace. 4. Fundamental tissues of Pinelliae Tuber, Typhonii Flagelliformis Rhizoma, and Arisaematis Rhizoma have starch grains and mucilage cells, and their mucilage cells include the raphides of calcium oxalate. Conclusion : Internal forms of Pinelliae Tuber, Typhonii Flagelliformis Rhizoma, and Arisaematis Rhizoma are difficult to distinguish from one another, but Pinelliae Tuber and Arisaematis Rhizoma can be distinguished by their external forms and sizes, and Pinelliae Tuber and Typhonii Flagelliformis Rhizoma were distinguished by the forms of apex.

• Biomolecules & Therapeutics
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• 제17권4호
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• pp.422-429
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• 2009

Invasion and metastasis is the main cause of cancer mortality. Angiogenesis is a prerequisite for the tumor growth and metastasis. Matrix metalloproteinases (MMPs) are the key enzymes playing in the invasive growth and metastasis of cancer as well as angiogenesis. Xanthohumol, a prenylated chalcone of the Hop plant (Humulus lupulus L), has been reported to suppress cancer invasion and angiogenesis. In the present study, we investigated the antiinvasive effects of xanthohumol (1) and its synthetic derivatives, 4'-O-methylxanthohumol SEM ether (2), xanthohumol C (3), and xanthohumol C MOM ether (4) in relation to MMP expression in HT-1080 human fibrosarcoma cells. The compound 1 and its derivative, 3 and 4, significantly inhibited serum-induced HT-1080 cell invasion, and 12-O-tetradecanoylphorbol-13-acetate (TPA)-enhanced activity and expression level of MMP-2 and MMP-9 in a concentration-dependant manner. In addition, they inhibited TPA-enhanced expression of MT1-MMP with relatively weak inhibition in tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2 level. The compound 1 significantly decreased the cell viability, whereas the derivatives, 2 and 3 showed no cytotoxicity, and compound 4 showed slight cytotoxicity in the cells. Furthermore, in a chick chorioallantoic membrane (CAM) assay, the derivatives 3 and 4 dose-dependently suppressed vascular endothelial growth factor (VEGF)-induced angiogenesis, which is similar to that of compound 1. Taken together, the results indicate that compounds 3 and 4 may be valuable anti-angiogenic agents in the treatment of chronic diseases such as cancer and inflammation working through suppression of MMP-2 and MMP-9.

• 한국컴퓨터산업학회논문지
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• 제8권1호
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• pp.23-28
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• 2007

현대인의 과로, 과음, 흡연으로 인한 근육에 스트레스를 주는 자세가 많다. 한 자세로 오래 앉아 있는 시간이 많으므로 근육통은 근육 류머티즘이나 결합 조직염, 근통증으로, 환부를 누르면 경결이 있고 아프다. 근육자체에는 병적변화가 없고 결합 조직염의 경우는 그 근육 주위에 있는 근육막이나 힘줄 신경초 등의 결합조직에 류머티즘 변화가 나타난다. 전형적인 비관절성 류머티즘이기도 하다. 과격한 운동이나 몸에 익숙지 않은 일을 무리하게 했을 경우에 볼 수 있다. 이로인해 본 연구에서는 설정된 주파수가 변화없이 지속되는 파형과 초음파 자극처리장치의 특성을 모드별 주파수 파형으로 연속모드, 10모드, 25모드, 50모드 등으로 다양하게 환자의 상태라 따라 구현이 가능하였다. 실험을 통해 초음파, 저주파장비에 대한 기초기준을 정할 수 있는 샘플을 확립 나름대로 정리하여 기준을 확립하고자 한다.

• BMB Reports
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• 제36권5호
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• pp.433-441
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• 2003

A cDNA clone for a salicylic acid-induced gene in Chinese cabbage (Brassica rapa subsp. pekinensis) was isolated and characterized. The cabbage gene, designated Br-sil1 (for $\underline{B}$rassica $\underline{r}$apa $\underline{s}$alicylate-$\underline{i}$nduced $\underline{l}$lipase-like 1 gene), encodes a putative lipase that has the family II lipase motif GDSxxDxG around the active site serine. A database search showed that plant genomes have a large number of genes that contain the family II lipase motif. The lipase-like proteins include a myrosinase-associated protein, an anther-specific proline-rich protein APG, a pollen coat protein EXL, and an early nodule-specific protein. The Br-sil1 gene is strongly induced by salicylic acid and a non-host pathogen, Pseudomonas syringae pv. tomato, that elicits a hypersensitive response in Chinese cabbage. Treatment of the cabbage leaves with BTH, methyl jasmonate, or ethephon showed that the Br-sil1 gene expression is induced by BTH, but not by methyl jasmonate or ethylene. This indicates that the cabbage gene is activated via a salicylic acid-dependent signaling pathway. An examination of the tissue-specific expression revealed that the induction of the Br-sil1 gene expression by BTH occurs in leaves and stems, but not in roots and flowers. Without the BTH treatment, however, the Br-sil1 gene is not expressed in any of the tissues that were examined.

• 한국환경복원기술학회지
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• 제23권3호
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• pp.63-79
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• 2020

The present study was to investigate the growth response and nutrient absorption in three willow species (Salix gracilistyla, S. koreensis and S. chaenomeloides) treated with nitrogen source (NH₄⁺:NO₃^- rate; 200:0, 150:50, 100:100, 50:150, 200:0) for a period of 90days. The height, dry weight and chlorophyll contents of three species of willows were found to be highest at 50:150 (NH₄⁺:NO₃^-) treatment. NO₃^--N was more effective than NH₄⁺-N in the early growth of three species of willows. The increase in percentage of NO₃^--N handling, T-N, NO₃^--N in plant tissue increased. The analysis of C, N and mineral content in various parts revealed that the amounts of C, N, K, Ca and Mg were higher in leaves than those in the stems and the roots. However, the amount of NO₃^--N and P were higher in roots than those in the leaves and the stems. Salix koreensis was excellent, followed by S. chaenomeloides and S. gracilistyla in absorption of nitrate nitrogen. Higher percentage of NO₃^--N, the amounts of T-N, NO₃^--N, P₂O₅, K, Na, Ca, and pH in soil were decreased.

• 한국식품영양학회지
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• 제22권2호
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• pp.159-165
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• 2009

E. japonica is a well-known medicinal plant in Japan. The leaves of E. japonica were reported to have a hypoglycemic action. However, seeds of E. japonica are discarded and not used. To elucidate for anti-diabetic effects of E. japonica, Type 2 diabetic mice were allocated to control group, E. japonica leaf, and seed extract group. Animals were fed a 2018S Teklad global 18% protein rodent diet. Animals were received daily oral injections of E. japonica leaf or seed extract at a dose of 200 mg/kg body weight for 6 weeks. Body weight, food intake and water intake, and total adipose tissue weight of animals were significantly reduced by feeding of E. japonica leaf extract. All E. japonica extract groups significantly decreased fasting blood glucose, glycosylated hemoglobin levels, size of adipocytes and serum adiponectins. However, they did not have a beneficial effect on the serum triglyceride and cholesterol in the diabetic animals. These results suggest that E. japonica seed and leaf extracts have a antidiabetic effect by controlling of blood glucose and decrease of size of adipocytes in db/db mice and seed extract is more effective in hypoglycemic action than leaf extract.

• 대한약침학회지
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• 제22권4호
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• pp.239-247
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• 2019

Objectives: Allium jesdianum (Aj) is a medicinal plant that has highlighted pharmacological features. In this study, the effects of Aj extract were examined on acetaminophen (APAP)-induced hepatic failure in rats. Methods: Methanolic fraction of hydro-alcoholic extract of Aj was obtained by silica gel column chromatography method. Animals were randomly divided into four groups each containing six rats and treated by gavage as follows: the first and second groups received normal saline, the third and fourth groups were received with 50 and 100 mg/kg of Aj extract, respectively. After two consecutive weeks, the groups 2-4 were given a single dose of APAP (2 g/kg). After 48 hours, blood and liver samples were collected for biochemical and histological examinations. Results: The findings of the study demonstrated that APAP caused a significant increase in ALT (P < 0.001), AST (P < 0.001), LDH (P < 0.001), ALP (P < 0.001) serum levels, hepatic lipid peroxidation (LPO; P < 0.001) and nitric oxide (NO; P < 0.001). In this regard, APAP led to the depletion of the total antioxidant capacity (TAC; P < 0.001), glutathione and total thiol groups (TTGs; P < 0.001), and structural change in the liver. In the Aj extract groups, a considerable improvement was found in the hepatic function alongside the histopathologic changes. Conclusion: This investigation indicated that the influential effects of Aj extract in APAP-induced hepatic failure might depend on its effect on improving oxidant/antioxidant balance in hepatic tissue.

• 한국지하수토양환경학회지:지하수토양환경
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• 제19권5호
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• pp.35-44
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• 2014

In this study, the bio-phytoremediation and phytoremediation technologies were applied to the soils contaminated with total petroleum hydrocarbons (TPH) and heavy metals to evaluate the remediation efficacy from May 2012 to December 2013. Poplar (Populus bonatii Levl.) and Sun Hemp (Crotalaria juncea L.) were selected and planted in phytoremediation practice. These plants were also utilized in the bio-phytoremediation practice, with the addition of earthworm (Eisenia fetida) and petroleum-degrading bacteria (Pseudomonos sp. NKNU01). Furthermore, physiological characteristics, such as photosynthesis rate and maximal photochemical yield, of all testing plants were also measured in order to assess their health conditions and tolerance levels in adverse environment. After 20 months of remedial practice, the results showed that bio-phytoremediation practice had a higher rate of TPH removal efficacy at 30-60 cm depth soil than that of phytoremediation. However, inconsistent results were discovered while analyzing the soil at 100 cm depth. The study also showed that the removal efficiency of heavy metals was lower than that of TPH after remediation treatment. The results from test field tissue sample analysis revealed that more Zinc than Chromium was absorbed and accumulated by the tested plants. Plant height measurements of Poplar and Sun Hemp showed that there were insignificant differences of growth between the plants in remediation plots and those in the control plot. Physiological data of Poplar also suggested it has higher tolerance level toward the contaminated soils. These results indicated that the two testing plants were healthy and suitable for this remediation study.

• Journal of Nutrition and Health
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• 제33권7호
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• pp.703-711
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• 2000

The purpose of this study was to investigate the effect of butanol(BuOH) fraction of Polygonatum odoratum with selenium tr-eatment on blood glucose levels and lipid peroxidations in streptozotocin(STZ) induced diabetric rats. Male Sprague-Dawly rats weighing(180-200g) were divided into five groups : normal STZ-control and three expreimental groups(P, odoratum group P, odo-Se group and Se group) Diabetes mellitus was induced by injection STZ in the tail vein at the dose of 45mg/kg B.W The BuOH fraction of Polygonatum odoratum(500mg/kg. B,W) given orally administered for 14 days. The Se treated group were fed a AIN-76 recommendation diet mixed with Na2Seo3(2mg/kg diet). Diabetic rats showed the lower weight gain compared to the normal rats. the plasma glucose levels of the P. odo-Se group were significantly lower than the other experimental groups. The plasma cholesterol levels were higher in STZ-control and Se groups compared toP.odoratum and P. odo-Se groups and HDL-cholesterol levels were increased in the diabetic experimental groups fed on BuOH fraction of P. odoratum with Se supplementation. The liver and muscle glycogen levels were not significantly differ among all groups. The plasma free fatty acid levels were lower in diabetic experimental groups fed on BuOh fraction of P. odoratum or Se sup-plementation than STZ-control and Se groups. Diabetics rats showed the higher levels of triglyceride in plasma andlower levels in liver compared with the normal group. Supplementation with Se decreased significantly the liver triglyceride level. The MDA levels in liver and kidney were significantly reduced in all the experimental groups. In conclusion administration of BuOH fraction of Polygonatuum odoratum with selenium supplementation reduced blood glucose levels and peroxdative tissue damage in STZ induced diabetic rats showing the possibility of preventiave and therapeutic use of the wild edible plant to the diabetes mellitus.

• 생물환경조절학회지
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• 제13권2호
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• pp.102-106
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• 2004

착색단고추 관비재배 시 토양수분함량이 생육 및 품질에 미치는 영향을 구명하여 생산성 향상을 위한 기초자료로 활용코져 수행하였다. 광합성속도 및 증산속도는 토양내 수분량이 적은 -30 ㎪에서 낮았으며, 토양의 pH와 EC는 수분함량에 따른 큰 변화 없이 안정적이었다. 식물체의 전질소 함량은 -30 ㎪에서 가장 낮았으며, 칼륨, 칼슘, 마그네슘은 -10 kPa에서 가장 높았고, 인산은 처리간에 유의성이 없었다. 정식 60일째의 생육은 토양수분함량이 낮아질수록 저조하였으나 정식 210일째는 토양수분함량간에 유의성이 없었다. 품질은 토양수분함량에 따른 유의성이 없었으나 당도만 -30 ㎪에서 $8.0^{\circ}$Brit로서 가장 높았다. 따라서 착색단고추 관비재배를 위한 토양수분의 함량은 -10 ㎪∼-20 ㎪에서 생육 및 품질이 양호하였다.