• Asian Pacific Journal of Cancer Prevention
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• 제17권6호
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• pp.2735-2739
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• 2016

Curcumin, is a polyphenol from Curcuma longa (turmeric plant), is a polyphenol that belongs to the ginger family which has long been used in Ayurveda medicines to treat various diseases such as asthma, anorexia, coughing, hepatic diseases, diabetes, heart diseases, wound healing and Alzheimer's. Various studies have shown that curcumin has anti-infectious, anti-inflammatory, anti-oxidant, hepatoprotective, thrombosuppressive, cardio protective, anti-arthritic, chemo preventive and anti-carcinogenic activities. It may suppress both initiation and progression stages of cancer. Anticancer activity of curcumin is due to negative regulation of inflammatory cytokines, transcription factors, protein kinases, reactive oxygen species (ROS) and oncogenes. This review focuses on the different targets of curcumin to treat cancer.

• 한국자원식물학회지
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• 제31권3호
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• pp.195-201
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• 2018

This study was executed to evaluate the phenolic content, DPPH radical scavenging rate, and the cytotoxic effect in human cancer cell, 3T3-L1 cell from C. lanceolata extracts at various ethanol concentration. Total polyphenol and flavonoid content of the C. lanceolata at various ethanol concentration showed the high amount in 70%, 100% ethanol extract. The DPPH radical scavenging activity progressively increased in a dose-dependent manner, and showed the highest in 100% ethanol extract. The cytotoxic effect against human cancer cell of the C. lanceolata was higher in 50% and 70% ethanol extracts. In particular, the cytotoxic effect in MCF-7 cell was relatively higher than in other cells. The $IC_{50}$ (concentration causing 50% cell death) value showed the highest on MCF-7 cell ($538.39{\mu}g/m{\ell}$ in 70% ethanol extract, and exhibited significant activity against Hela cell ($637.87{\mu}g/m{\ell}$, Calu-6 cell ($728.64{\mu}g/m{\ell}$. The extract of 70% ethanol at $1,000{\mu}g/m{\ell}$ exhibited a pronounced cytotoxic effect on 3T3-L1 cell comparable to that of the other extracts, and reduced in a concentration-dependent manner.

• 한국자원식물학회지
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• 제28권6호
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• pp.759-766
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• 2015

The purpose of this study was to improve the functionality of a healthy drink with examining the possibility of manufacturing different enzymes (alpha-, beta-, glucose-amylase) in barley malts (BM) produced in various malting periods. The study showed that enzyme treatment increased significantly total polyphenol content (TPC), DPPH radical scavenging activity and hydroxly radical scavenging activity in malted liquid samples (MLS) which obtained from various malting periods. The highest of TPC were found in Gluco-24M with 1.981 mgTAE/ml, followed by Beta-24M and Alpha-72M with 1.878 mgTAE/ml and 1.845 mgTAE/ml, respectively. The DPPH result revealed that percent of inhibition increased by 71-75% compared to the control. No statistical difference was found between MLS obtained by 24 hr of malting (24 M) and 72 hr of malting (72 M) after enzyme treatment. In addition, an increasing of hydroxyl radical was in the same trend to the TPC and DPPH. The hydroxyl radical scavenging activity of enzyme treated samples was 1,5 times higher than the control. These results suggest the possibility of enzyme application to barley malts obtained in various germination periods for improving quality and functionality of barley malts.

• 한국환경농학회지
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• 제41권1호
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• pp.50-54
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• 2022

BACKGROUND: Phyllospadix japonica Makino is a perennial plant belonging to the family Zosteraceae. This species is native to the eastern coast of the Korean Peninsula, and it is found attached to rocks on the seashore. As with all seagrass species, P. japonica is also known to play a major role in protecting the marine environment, and studies on its physiological activities suggestive of its antioxidant, antibacterial and anti-inflammatory potentials have been reported. In this study, purification and structural analysis were performed to identify the polyphenol substances derived from P. japonica. METHODS AND RESULTS: An polyphenol substance MP-1 was purified from the 70% aqueous methanol extract of P. japonica using Diaion® HP-20 column chromatography, ODS column chromatography, and medium-pressure liquid chromatography (MPLC). The purified MP-1 was identified as rosmarinic acid having a molecular weight of 360 and a molecular formula of C18H16O8 through electrospray ionization (ESI)-mass and nuclear magnetic resonance (NMR) spectroscopic analysis. CONCLUSION(S): This study highlights the processes used for the identification of the polyphenol substance derived from P. japonica. Rosmarinic acid, the polyphenol derived from P. japonica identified by this study, is a kind of bioactive substance mainly present in plants. These findings provide an important starting point and are valuable for future studies on bioactive substances in seagrass.

• Journal of Microbiology and Biotechnology
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• 제32권2호
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• pp.160-169
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• 2022

In the present study we evaluated the efficacy of a bioformulation of Streptomyces cameroonensis for control of black pod disease in cocoa and enhancement of seedling growth. The formulation developed using talc powder and cassava starch as carriers showed high shelf-life of 1.07 × 10⁶ CFU/g after six months storage at 4℃. The formulation was tested for inhibition of spore germination in Phytophthora megakarya and showed 100% inhibition at 10% (w/v) of formulation. To determine the efficacy of the formulation, we performed an in planta assay in the greenhouse on two hybrids of cocoa seedlings, the tolerant SNK413 × (♂) T79/467 and the susceptible UPA 134× (♂) SCA 12. Detached leaf assay showed a significant reduction in the disease severity index of about 67% for the tolerant hybrid and 55% for the susceptible hybrid compared to non-treated plants. A significant enhancement in stem length, leaf surface area and root weight was observed. Analysis of biochemical markers of defense showed a significant increase in total polyphenol, flavonoid, and total protein contents. There was also significant upregulation of PR-proteins such as chitinases, peroxidases and β-1, 3-glucanases following treatment of both tolerant and susceptible hybrids, though with a higher level of synthesis in the tolerant hybrids. A significant increase was also observed in polyphenol oxidase activities in plants treated with the formulation. This work demonstrated the stability and effectiveness of the S. cameroonensis powder formulation in suppressing black pod disease in cocoa and subsequently enhancing the growth of seedlings.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.212.3-213
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• 2003

Polyphenolic compounds including flavonoids are wide spread in the plant kingdom, and interested recently because epidemiological studies have suggested correlations between the consumption of polyphenol-rich plant foods and the prevention of chronic diseases. Soy is a main source of isoflavonoids which are high dietary intake for the oriental population. In this study, anti-inflammatory action of sophoricoside, an isoflavone glycoside isolated from immature fruits of Sophora japonica (Leguminosae family), has been demonstrated. (omitted)

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.95.1-95
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• 2003

Plant growth promoting rhizobacteria (PGPR) have been shown to suppress phytopthora blight. This suppression has been related to both microbial antagonism and induced resistance. The PGPR isolates were screened by dual culture plate method and most of the isolates were showed varying levels of antagonism. Among the PGPR isolates pyoverdin, pyochelin and salicylic acid producing strains showed the maximum inhibition of mycelial growth of Phytopkhora capsici and increased plant growth promotion in red pepper. PGPR isolates further analysed for its ability to induce production of defence related enzymes and chemicals. The activities such as Phenyle alanin ammonia Iyase (PAL), Peroxidase (PO), Polyphenol oxidase (PPO) and accumulation of phenolics were observed in PGPR pretreated red pepper plants challenged with Phytopkhora capsici. The present study shows that an addition of direct antagonism and plant growth promotion, induction of defense related enzymes involved to enhance resistance against invasion of P. capsici in red pepper.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 1997년도 Proceedings of special lectures on Recent Research Trend of Plant Pathology
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• pp.47-55
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• 1997

Plant growth promoting fungi(PGPF) obtained from zoysiagrass rhizosphere offer dual advantages - induse systemic disease resistance response in cucumber to C. orbiculare infection and cause enhancement of plant growth and increase yield. PGPF protected plants either by colonizing roots or by their metabolites. PGPF offer an advantage by protecting plants for more than 9 weeks and 6 week in the greenhouse and field. PGPF-induced plants limited pathogen spore germination and decreased the number of infection hyphae on the leaf, and increased lignification at places of attempted pathogen infection, thus reducing the pathogen spread. PGPF elicited increased activities of chitinascs, glucanases, peroxidase, polyphenol oxidase, and phenylalanine ammonia lyase to C. orbiculare infection in cucumber plants. The role of PGPF in elevating cucumber defense response to pathogen infection suggests potential application of PGPF as biological control agents.

• 한국자원식물학회지
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• 제37권2호
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• pp.110-119
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• 2024

본 연구는 꼬리진달래의 자원식물 개발을 위한 정보를 제공하고자 수행되었다. 꼬리진달래 신초 추출물의 폴리페놀 프로파일링을 통해 유용성분을 선발하고, 추출용매에 따른 유용성분 함량과 항산화 활성을 비교하였다. 꼬리진달래 신초 추출물에서 37개의 폴리페놀 화합물을 확인하였으며, chlorogenic acid, astragalin, myricetin, afzelin의 추출용매별 함량을 비교한 결과, chlorogenic acid의 함량은 에탄올 추출물에서 6.57±0.12 mg/g이었고, astragalin의 함량은 에탄올 추출물에서 2.29±0.02 mg/g, myricetin의 함량은 메탄올 추출물에서 4.77±0.06 mg/g, afzelin의 함량은 메탄올 추출물에서 0.10±0.01 mg/g이었다. 꼬리진달래 에탄올 추출물의 총 폴리페놀 함량은 80.01±2.36 mg/g, 총 플라보노이드 함량은 78.08±3.44 mg/g이었다. 또한 꼬리진달래 메탄올 추출물의 DPPH radical 소거활성 IC₅₀값은 943.57±10.68 mg/L였으며, ABTS radical 소거활성 IC₅₀은 641.60±7.58 mg/L였다. 꼬리진달래는 유기용매 추출 시 유용성분과 항산화 활성이 높았고, 동일 속의 종들과 비교하여 높은 함량의 chlorogenic acid, astragalin, myricetin, afzelin을 함유하고 있어 향후 건강기능성 소재로의 개발이 가능할 것으로 판단된다.

• 한국식품영양과학회지
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• 제39권5호
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• pp.769-775
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• 2010

본 실험에서는 국내에 자생하는 콩과에 속하는 6종의 싸리속 식물의 각 부위별 추출물로부터 총 폴리페놀, 총 플라보노이드 함량을 측정하였고, DPPH, ABTS 라디칼 소거능을 조사하였다. 폴리페놀성 화합물은 고양싸리의 잎(LR-L)에 가장 많이 포함되어 있는 것으로 조사되었고, 플라보노이드 화합물은 풀싸리의 지상부(LTi-A)에서 가장 많이 검출되었다. 이들 시료의 항산화 활성을 조사하기 위해서 DPPH와 ABTS 라디칼에 대한 소거 활성을 조사해 본 결과 DPPH 라디칼에 대해서는 비타민 C보다 소거 활성이 적은 것으로 나타났지만, ABTS 라디칼에 대해서는 비타민 C보다 더 좋은 소거 활성을 보였다. 이들 활성과 폴리페놀, 플라보노이드 화합물간의 상관관계에서는 폴리페놀만이 양의 상관관계를 보여 폴리페놀의 농도에 따라 라디칼 소거 활성이 증가하는 것을 관찰할 수 있었다. 하지만 플라보노이드 화합물과 라디칼 소거 활성 간에는 뚜렷한 상관관계를 볼 수 없어, 싸리속 식물에서는 플라보노이드 화합물보다는 폴리페놀성화합물이 항산화에 더욱 중요한 역할을 하는 것으로 판단할 수 있었다. 이러한 결과는 최근 각광을 받고 있는 천연 항산화제의 개발에 있어 싸리속 식물이 천연 항산화제로서 좋은 소재가 될 수 있음을 보여주고 있다.