Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
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Identification of Polyphenol Substances (MP-1) from Seagrass, Phyllospadix Japonica Makino

잘피 게바다말로부터 폴리페놀 물질(MP-1)의 분리 및 동정

  • Kim, Hae-Seon (Marine Industry Research Institute for East sea rim) ;
  • Park, Nyun-Ho (Marine Industry Research Institute for East sea rim) ;
  • Suk, Ho-young (Department of Life Sciences, College of Natural Sciences, Yeungnam University) ;
  • You, Sang-guan (Department of Marine Food Science and Technology, College of Life Sciences, Gangneung-Wonju National University) ;
  • Woo, Jung-Hee (Marine Industry Research Institute for East sea rim)
  • 김해선 (환동해산업연구원) ;
  • 박년호 (환동해산업연구원) ;
  • 석호영 (영남대학교 자연과학대학 생명과학과) ;
  • 유상권 (강릉원주대학교 생명과학대학 해양식품공학과) ;
  • 우정희 (환동해산업연구원)
  • Received : 2022.03.10
  • Accepted : 2022.03.24
  • Published : 2022.03.31
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Abstract

BACKGROUND: Phyllospadix japonica Makino is a perennial plant belonging to the family Zosteraceae. This species is native to the eastern coast of the Korean Peninsula, and it is found attached to rocks on the seashore. As with all seagrass species, P. japonica is also known to play a major role in protecting the marine environment, and studies on its physiological activities suggestive of its antioxidant, antibacterial and anti-inflammatory potentials have been reported. In this study, purification and structural analysis were performed to identify the polyphenol substances derived from P. japonica. METHODS AND RESULTS: An polyphenol substance MP-1 was purified from the 70% aqueous methanol extract of P. japonica using Diaion® HP-20 column chromatography, ODS column chromatography, and medium-pressure liquid chromatography (MPLC). The purified MP-1 was identified as rosmarinic acid having a molecular weight of 360 and a molecular formula of C18H16O8 through electrospray ionization (ESI)-mass and nuclear magnetic resonance (NMR) spectroscopic analysis. CONCLUSION(S): This study highlights the processes used for the identification of the polyphenol substance derived from P. japonica. Rosmarinic acid, the polyphenol derived from P. japonica identified by this study, is a kind of bioactive substance mainly present in plants. These findings provide an important starting point and are valuable for future studies on bioactive substances in seagrass.

Keywords

Acknowledgement

This study was carried out with the supports of R&D program (Project No. FTIS 2021407D10-2125-0101) provided by the Ministry of Oceans and Fisheries, Korea Forest Service (Korea Forestry Promotion Institute) and Gyeongsangbuk-do R&D project.

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