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http://dx.doi.org/10.7732/kjpr.2015.28.6.759

Barley Malt Treated with Enzymes Increases Polyphenol Content and Antioxidant Activity  

Phouthaxay, Phonesavanh (Department of Bio-Health Technology, Kangwon National University)
Yu, Chi Young (Plant Square Co.)
Pang, Yeon Gyu (Department of Biosystem Engineering, Kangwon National University)
Salitxay, Timnoy (Department of Bio-Health Technology, Kangwon National University)
Kim, Sang Heon (Department of Biosystem Engineering, Kangwon National University)
Park, Cheol Ho (Department of Bio-Health Technology, Kangwon National University)
Publication Information
Korean Journal of Plant Resources / v.28, no.6, 2015 , pp. 759-766 More about this Journal
Abstract
The purpose of this study was to improve the functionality of a healthy drink with examining the possibility of manufacturing different enzymes (alpha-, beta-, glucose-amylase) in barley malts (BM) produced in various malting periods. The study showed that enzyme treatment increased significantly total polyphenol content (TPC), DPPH radical scavenging activity and hydroxly radical scavenging activity in malted liquid samples (MLS) which obtained from various malting periods. The highest of TPC were found in Gluco-24M with 1.981 mgTAE/ml, followed by Beta-24M and Alpha-72M with 1.878 mgTAE/ml and 1.845 mgTAE/ml, respectively. The DPPH result revealed that percent of inhibition increased by 71-75% compared to the control. No statistical difference was found between MLS obtained by 24 hr of malting (24 M) and 72 hr of malting (72 M) after enzyme treatment. In addition, an increasing of hydroxyl radical was in the same trend to the TPC and DPPH. The hydroxyl radical scavenging activity of enzyme treated samples was 1,5 times higher than the control. These results suggest the possibility of enzyme application to barley malts obtained in various germination periods for improving quality and functionality of barley malts.
Keywords
Barley malt; Malting period; Enzymatic treatment; Antioxidant; Total polyphenol;
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Times Cited By KSCI : 5  (Citation Analysis)
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1 Bu, S.Y., W.B. Jeon, D.S. Kim, H.Y. Hoe and Y.W. Seo. 2002. Antioxidant activity and total phenolic compounds in grain extracts of wheat, barley, and oat. Korean J. Crop Sci. 47(2):201-207.
2 Bryjak, J. 2003. Gluco-amylase, alpha-amylase and beta-amylase immobilisation on acrylic carriers. Biochemical Engineering Journal 16:347-355.   DOI
3 Braca, A.G., I. Morelli, F. DeiSimone and N.D. Tommasi. 2003. Antioxidant and free radical scavenging activity of flavonol glycosides from different Aconitum species. J. Ethnopharmacol. 86:63-67.   DOI
4 Beta, T., S. Nam, J.F. Dexter and H.D. Sapirstein. 2005. Phenolic content and antioxidant activity of pearled wheat fraction. Cereal Chemistry 82(4):390-393.   DOI
5 Lee, N.Y., Y.K. Kim, I. Choi, S.K. Cho, H.J. Kweon, K.W. Lee, J.N. Hyun, J.S. Choi, K.H. Park, K.J. Kim and M.J. Lee. 2010. Antioxidant activity and biological activity of specialty barley (Horduem vulgare L.) extracts. Korean J. Crop Sci. 55(2):159-164.
6 Lee,Y.T., S.J. Seo and H.G. Chang. 1999. Quality characteristics of barley varieties related to enzymatic activity in malt. Korean J. Food Sci. Technol. 31(6):1421-1426 (in Korean).
7 Laseken, O.O. 1999. Effect of Vernonai amygdalina (bitter leaf) extract on brewing qualities and amino acid profiles of stout drinks from sorhum and barley malts. Food Chem. 64:507-510.   DOI
8 Halliwell, B., J.M.C.Guttridge and O.I. Aruoma. 1987. Analytical Biochemistry 165:215-219.   DOI
9 Jang, H.J., H.J. Bu and S. Lee. 2015. Screening for antioxidative activity of Jeju native plants. Korean J. Plant Res. 28(2):158-167.   DOI
10 Fogarasi, A.L. 2015. A comparative assessment of antioxidant properties, total phenolic content of einkorn, wheat, barley and their malts. Food Chem. 167:1-6.   DOI
11 Evers, A.D. and E.E. McDermot. 1970. Scanning electron microscopy of wheat starch. II. Structure of granules modified by α-amylolysis-preliminary report. Staerke 22:23.   DOI
12 Zielinski, H. and H. Kozlowska. 2000. Antioxidant activity and total phenolics in selected cereal grains and their different morphological fraction. J. Agric. Food Chem. 48:2008-2016.   DOI
13 Cook, N.C. and S. Samman. 2006. Flavonoids-chemistry, metabolism, cardio protective effect, and dietary sources. Nutr. Biochem. 7:66-76.
14 Chon, S.U and Y.M. Kim. 2006. Phytotoxicity and DPPH radical scavenging activity of barley seedling extracts. Korean J. Crop Sci. 51(4):322-328.
15 Chon, S.U., T.S. Kim, J.S. Shin and H.O. Boo. 2008. Antioxidant activity of methanol extracts from root part of Korean salad plants. Korean J. Plant Res.12(6):413-419.
16 Singleton, V.L. and J.A. Rossi. 1965. Calorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. American J. of Enology and Viticulture 16:144-158.
17 Seog, H.M., M.S. Seo, S.R. Kim., Y.K. Park and Y.T. Lee. 2002. Characteristics of barley polyphenol extract (BPE) separated from pearling by-products. Korean J. Food Sci. Technol. 34(5):775-779 (in Korean).
18 Ryu, S.N., S.Z. Park and H.Y. Kim. 2002. Antioxidant activity in rice cultivar, wild rice, and barley. Korean J. Crop Sci. 47(1):54-61.
19 Qingming, Y., P. Xianhui, K. Weiboa,Y. Hong and S.Yidan. 2010. Antioxidant activity of malt extract from barley (Hordeum vulgare L.) toward various oxidative stress in vitro and in vivo. Food Chem. 188:84-89.
20 Maillard, M.N., M.H. Soum, P. Boivin and C. Berset. 1996. Antioxidant activity of barley and malt: Relationship with phenolic content. Lebensmittel Wissenschaft and Technologie. 29:238-244.   DOI