• Journal of Crop Science and Biotechnology
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• v.11 no.1
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• pp.51-56
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• 2008

Azuki bean has never been commercially grown in Thailand, due in part to a lack of suitable varieties. A core collection of 114 azuki bean accessions, originally from different parts of China(northern, central, southern) representing the germplasm of Chinese land races, were evaluated in the experimental field of the Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, China from June to October 2004. The same experiment was repeated at Kamphaeng Saen campus of Kasetsart University, Thailand from February to May 2005. Yield, yield components, and agronomic traits were recorded in all accessions in order to identify certain genotypes for further investigation. The statistical parameters that were used as indicators of phenotypic variation were mean, coefficient of variability(CV), correlation coefficient(r), range, mean difference, and phenotypic clustering of the accessions. The results indicated that the azuki bean varieties planted in Kamphaeng Saen were shorter, earlier in growing duration, and lower in plant height, seed yield per plant, 100-seed weight, and pods per plant as compared to when they were grown in China. This discrepancy was caused largely by the combined effect of temperature, rainfall, and day length. The traits that were rather stable in both locations were branches per plant and seeds per pod. Azuki bean varieties from northern China showed higher response to the changing environments compared with those from central and southern China. Some agronomic traits showed high correlation coefficient between the environments in Thailand and China. The CV of agronomic traits in both locations were ranked in descending order as follows: seed yield per plant, pods per plant, branches per plant, plant height, 100-seed weight, seeds per pod, and growing duration. The CV of seeds per pod and branches per plant were almost the same in both locations. Yield per plant in China correlated well(r=0.75) with pods per plant, but not with the other traits. Based on their response to both environments, the azuki bean accessions can be broadly divided into four groups, viz. northern 1, northern 2, central, and southern. This implied that there was more diversity, but probably less stability among the accessions originating from northern China.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2002.11a
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• pp.29-30
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• 2002

The goal of this research was to develop the effectiveness of in vitro culture method for A. formosanus and study the environment in vitro conditions affecting on growth. The first series of experiments were examined to investigate the response of three different basal media, MS (Murashige and Skoog, 1962), Knudson (KC; Knudson, 1946) and modified hyponex on growth and multiplication during in vitro culture. Multiple shoot proliferation was induced in shoot tip explants on Hyponex (H3) media supplemented with BA (1 mg1$\^$-1/) or TDZ (1-2 mg1$\^$-1/). Addition of activated charcoal (1%) to the TDZ containing medium promoted rapid shoot tip proliferation (11.1 shoots per explant) but the same medium had an opposite effect resulting in poor proliferation in the nodal explants. However, the regenerated shoots had slow growth rate and failed to elongate. This problem was overcome by transferring the shoot clumps to a hormone free H3 media supplemented with 2% sucrose and 0.5% activated charcoal. Using bioreactor culture for scaling up was also shown the best way for multiple shoot induction and growth of this plant. The second series of experiments was studied to investigate the effect of physical environment factors on growth of in vitro plantlets. The Anoectochilus formosanus plantlets were cultured under different air exchange rate (0.1, 0.9, 1.2h$\^$-1/), without sucrose or supplement 20g.1$\^$-1/ (photoautotrophic or photomixotrophic, respectively), and different photosynthesis photon flux (40, 80, 120 ,${\mu}$mol.m$^2$.s$\^$-1/- PPF). Under non-enrichment CO$_2$ treatment, slow growth was observed in photoautotrophical condition as compared with photomixotrophical condition on shoot height, fresh weigh and dry weight parameters; High air exchange (1.2.h-l) was found to be inadequate for plant growth in photomixotrophical condition. On the contrary, under CO$_2$, enrichment treatment, the plant growth parameters were sharply (visibly) improved on photoautotrophic treatments, especially on the treatment with air exchange rate of 0.9.h-1. The growth of plant in photoautotrophic condition was not inferior compared with photomixotrophic, and the best growth of plantlet was observed in treatment with low air exchange rate (0.9.h-1). Raising the PPF level from 80 to 120${\mu}$mol.m$\^$-2/.s$\^$-1/ decreased the plant height, particularly at 120${\mu}$mol.m$\^$-2/.s$\^$-1/ in photoautotrophic condition, fresh weight and dry weight declined noticeably. At the PPF of 120${\mu}$mol.m$\^$-2/,s$\^$-1/, chlorophyll contents lowed compared to those grown under low PPF but time courses of net photosynthesis rate was decreased noticeably. Light quality mainly affected morphological variables, changes of light quality also positively affected biomass production via changes in leaf area, stem elongation, chlorophyll content. Plant biomass was reduced when A. formosanus were grown under red LEDs in the absence of blue wavelengths compare to plants grown under supplemental blue light or under fluorescent light. Stem elongation was observed under red and blue light in the present experiment. Smaller leaf area has found under blue light than with other lighting treatments. Chlorophyll degradation was more pronounced in red and blue light compared with white light or red plus blue light which consequent affected the photosynthetic capacity of the plant. The third series of experiment were studied to investigate the effect of physical environment factors on growth of ex vitro plants including photosynthesis photon flux (PPF), light quality, growing substrates, electrical conductivity (EC) and humidity conditions. In the present experiments, response of plant on PPF and light quality was similar in vitro plants under photosynthesis photon flux 40${\mu}$mol.m,$\^$-2/.s$\^$-1/ and white light or blue plus red lights were the best growth. Substrates testing results were indicated cocopeat or peat moss were good substrates for A. formosanus growth under the greenhouse conditions. In case of A. formosanus plants, EC is generally maintained in the range 0.7 to 1.5 dS.m-1 was shown best results in growth of this plant. Keeping high humidity over 70% under low radiation enhanced growth rate and mass production.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 2000.04a
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• pp.97-104
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• 2000

Evaluating a Review-Level Ground Motion is a key to efficiently perform Seismic Margin Assessment of nuclear power plants whose purpose is to determine a ground motion level for which a plant has high-confidence-of-a-low-probability of seismic-induced core damage and to identify any weaker-link components. In this study a method to obtain RLGMs is reviewed which is recommended by Electric Power Research Institute and implemented to be applied to Limerick site in eastern and central U. S as a case study. This method provides reasonable and site-specific RLGMs as minimum required plant HCLPF for SMA that meet a target mean seismic core-damage frequency based on seismic hazard results and generic values of uncertainty and randomness parameters of the core-damage fragility curves. In addition high-frequency RLGM is justifiably modified to reflect the increased seismic capacity of high-frequency components and spatial variation and incoherence of input ground motion on a basemat of large structures by establishing a method to obtain high0-frequency reduction factors according to EPRI guidelines.

• Journal of Electrical Engineering and Technology
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• v.10 no.1
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• pp.395-400
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• 2015

Obtaining the parameters for PID controllers based on limit cycle information for the process in a relay controlled feedback loop has become an accepted practical procedure. If the form of the plant transfer function is known, exact expressions for the limit cycle frequency and amplitude can be derived so that their measurements, assumed error free, can be used to calculate the true parameter value. In the literature, parameter estimation for an assumed form of the plant transfer function has generally been considered for disturbance free cases, except a recently published work of the author. In this paper additional simulation results are reported on exact parameter estimation from relay autotuning under static load disturbances.

• 제어로봇시스템학회:학술대회논문집
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• 2004.08a
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• pp.1500-1505
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• 2004

This paper presents the design and control of a small Brushless DC (BLDC) Motor for entertainment robots. In order to control the developed BLDC motor, Adaptive Fuzzy Control (AFC) scheme via Parallel distributed Compensation(PDC) is developed for the multi- input/multi-output plant model represented by the Takagi-Sugeno(TS) model. The alternative AFC scheme is proposed to provide asymptotic tracking of a reference signal for the systems with uncertain or slowly time-varying parameters. The developed control law and adaptive law guarantee the boundedness of all signals in the closed-loop system. In addition, the plant state tracks the state of the reference model asymptotically with time for any bounded reference input signal. The suggested design technique is applied to the velocity control of a developed small BLDC motor for entertainment robots.

• 제어로봇시스템학회:학술대회논문집
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• 2004.08a
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• pp.256-261
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• 2004

This paper presents a simple two-degree of freedom PID tuning table based on the CDM design method. The structure of the control system will be composed of plant, P or PI or PID controller and a pre-filter. The finalized formula can be used based on the experimental test of the plant in the same manner as the Ziegler-Nichols' second method. That is; users just need to find the critical gain and critical period experimentally and the parameters of the P, PI or PID controller with the pre-filter can be obtained by substituting the values of critical gain and critical period in the tuning table. The simulation results of the control systems utilizing the proposed controllers compared with those using the Ziegler-Nichols' second method will also be demonstrated.

• 제어로봇시스템학회:학술대회논문집
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• 2004.08a
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• pp.536-539
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• 2004

In this paper, a brief discussion on I-PD position controller design for step motor drive is presented. The proposed method mainly focuses on the robustness property of the controller, which is very important for this type of system in which the variation of external load affects plant parameters. It is considered in this paper that two types of controller design methods namely; Coefficient Diagram Method (CDM), and arbitrary Pole Assignment Method (PAM) are treated and compared them. The control plant chosen for our study is a SM inherently is comprised of some non-linear elements. A the scope of the design method is limited to only linear time invariant systems, the SM modeling is approximated to linear system.

• Asian Journal of Turfgrass Science
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• v.15 no.1
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• pp.9-14
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• 2001

Callus formation and plant regeneration from the seeds of zoysiagrass cv. Zenith was tested on MS basal medium supplemented with various concentrations of 2,4-dichlorophenoxyacetic acid(2,4-D) and of several cytokinins. A concentration of 1mg/L 2,4-D on medium stimulated callus formation. In the presence of 5mg/L 2,4-D, addition of 1mg/L kinetin significantly enhanced callus formation and plant regeneration over 2,4-D alone. To transfer foreign DNA into turfgrass, parameters for the bombardment of embryogenic callus with the particle bombardment were partially optimized using transient expression assay of a $chimeric \beta$-glucuronidase(GUS) gene driven by the CaMV 35S promoter. GUS gene was strongly expressed at helium pressure 1,100 psi and 6~9cm target distance.

• The Korean Journal of Ecology
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• v.24 no.6
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• pp.381-384
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• 2001

A preliminary comparative histodimensional studies, such as wood to bark ratio, total area of parenchyma cells and bast fibers, height and diameter of vascular rays, pith diameter and phenological parameters, height of the plant, perimeter of the stem, dry weight of the stem and leaves have been investigated for the low and high rubber bearing Guayule plants growing in same and different climatic regions of India. A plausible correlation is found between the percentage of rubber content and anatomical characters in the investigated varieties.

• Journal of Environmental Science International
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• v.19 no.12
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• pp.1335-1341
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• 2010

Voltammetric diagnostics of pesticide thiram was studied in plant leafs in vivo fluid with DNA immobilized on a carbon nanotube electrode (DCE). Sensor properties of carbon nanotube (CE) and DNA immobilized nanotube were compared. DCE was more effective than CE in target detecting. The parameters such as pH strength, stripping accumulation, amplitude, and increment potential were examined to find the optimum condition for detection of pesticide thiram in a sesame leaf. The optimized conditions were as follows 550 Hz frequency, 0.15 V amplitude, 0.005 V increment potential, -1.2 V initial potential, 4.78 pH, 500 sec accumulation time. Under optimum condition, the detection limit of thiram was attained at 0.01ng/L.