• Journal of Nutrition and Health
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• v.49 no.2
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• pp.63-71
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• 2016

Purpose: Skin pH, an indicator of skin health, is maintained by various organic factors, which include lactate, free amino acid (FAA), and free fatty acid (FFA). As skin ages or with illness, skin pH becomes less acidic, and functional food has been developed to maintain the acidic pH of skin. In this study, we determined the dietary effect of green tea extract (GTE) on skin pH of photo-aged mice, as measured by epidermal levels of lactate, FAA, and FFA. The protein expression and activity of lactate dehydrogenase (LDH), an enzyme of pyruvate reduction for lactate generation, was further determined. Methods: Albino hairless mice were fed a control diet (group UV+) or a diet with 1% GTE (group GTE) in parallel with UV irradiation for 10 weeks. A normal control group was fed a control diet without UV irradiation for 10 weeks (group UV-). Results: Skin pH was higher (less acidic) in group UV+ than in group UV-. In parallel, epidermal levels of lactate and FFA, as well as of LDH protein expression and activity, were reduced in group UV+. Dietary supplementation of GTE (group GTE) reduced skin pH to similar to the level of group UV-, and inversely increased epidermal levels of lactate, LDH protein expression and activity, but not of FFA. Although epidermal levels of FAA were similar in groups UV- and UV+, it was increased in group GTE to a level higher than in group UV-. In further analysis of major FFA, epidermal levels of palmitic acid [16:0], oleic acid [18:1(n-9)], and linoleic acid [18:2(n-6), but not of stearic acid [18:0] in group GTE were similar to or lower than those in group UV+. Conclusion: Dietary GTE normalized skin pH with increased levels of lactate and FAA, as well as with increased protein expression and activity of LDH in the epidermis of UVB irradiated hairless mice.

• Microbiology and Biotechnology Letters
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• v.41 no.4
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• pp.449-455
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• 2013

Human skin is constantly exposed to environmental conditions such as UV rays, polluted air, and chemical products. UV rays, in particular, affect skin in many ways causing wrinkles, fine wrinkles, rough skin, and xeroderma through a skin aging process. The purpose of this study was to investigate the anti-wrinkling effect of Juniperus rigida Sieb., derived from a common cedar tree found the world over. Measuring the elastase to investigate wrinkling efficacy, it was shown that at a concentration level of $1,000{\mu}g/ml$ of the two extracts, the water extract exhibited a lower than 10% inhibition activity, while the ethanol extract exhibited a 68.5% inhibition activity. Collagenase inhibition activity in the water extract and ethanol extract were 44.9% in the former and 97.2% in the latter extract, which in the case of the ethanol extract, is similar to ascorbic acid (99.6%). Moreover, measuring the biosynthesis of collagen by fibroblast, a concentration level of $50{\mu}g/ml$ of ethanol extract produced 151.52% of biosynthetic promotion, proving that the ethanol extract acts as a superb anti-wrinkling agent. The result of an investigation conducted on the influence of the ethanol extract on MMP-1 caused by UVA showed that at a concentration level of $1,00{\mu}g/ml$ of the ethanol extract of J. rigida Sieb a 67.1% inhibition activity was noted. At a concentration level of $50{\mu}g/ml$ of the ethanol extract of J. rigida Sieb a 35% and 39% inhibition ratio to MMP-1 protein and mRNA were observed respectively, thereby restraining the appearance of the collagen breakdown enzyme MMP-1 and wrinkle creation by skin photo-aging.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.42 no.1
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• pp.75-85
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• 2016

Pectin, a naturally occurring polysaccharide, has in recent years attracted considerable attention. Its benefits are increasingly appreciated by scientists and consumers due to its safety and usefulness. The chemistry and gel-forming characteristics of pectin have enabled to be used in pharmaceutical industry, health promotion and treatment. Yet, it has been rarely used in cosmetics because of its incompatibility with many cosmetic ingredients, including alcohols, and unstable viscosity of pectin gels under various pH and salt conditions. However, low-molecular-weight pectin oligomers have excellent biological activities, and depolymerization of pectin to produce cosmetic ingredients would be very useful. In this study, we attempted the development of cosmetic ingredients using pectin with an excellent effect on human skin. We developed a bio-conversion process that uses enzymatic hydrolysis to produce pectin hydrolysates containing mainly low-molecular-weight pectin oligomers. Gel permeation chromatography was used to determined the ratio of hydrolysis. The molecular weight of the pectin hydrolysates obtained varied between 200 and 2,700 Da. The two newly developed low-molecular-weight pectin hydrolysates, LMPH A and B, had higher anti-oxidative activities than pectin or D-galacturonic. Exposure to UVB radiation induces apoptotic cell death in epidermal cells. Annexin V binding and propidium iodide uptake were measured by flow cytometry to evaluate UVB-induced cell death in HaCaT cells. Both LMPH A and B reduced UVB-induced cell death and increased cell proliferation by 22% and 30% at 0.5% concentration respectively, while pectin had no significant activity. In conclusion, this study suggests that the newly developed low-molecular-weight pectin hydrolysates can be used as safe and biologically active cosmetic ingredients.

• Journal of Fisheries and Marine Sciences Education
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• v.26 no.5
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• pp.935-940
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• 2014

To investigate effect of on sexual maturation in spotted seabass, Lateolabrax maculatus the sexual maturation, photoperiods were established to natural photoperiod, 9L:15D, 11L:13D, 13L:11D and 15L:9D. After rearing for 90 days from August 11 to November 10, gonadosomatic index (GSI) and hormones were analyzed. In the female, GSI was $1.07{\pm}0.09%$ in the natural photoperiod, $1.93{\pm}0.06%$ in 9L:15D, while the GSI of male was $0.75{\pm}0.05%$ in the natural photoperiod, $1.89{\pm}0.12%$ in 9L:15D and $0.22{\pm}0.05%$ in 15L:9D and the lowest in 15L:9D, respectively. The blood estradiol (E2) of female was $226.0{\pm}13.6pg/ml$ in the natural photoperiod, and the highest value of $296.3{\pm}15.0pg/ml$ in 9L:15D. The male $17{\alpha}20{\beta}OHP$ was similar to the female with $196.3{\pm}17.0pg/ml$ in the natural photo period and $133.0{\pm}15.5pg/ml$ in 9L:15D. The testosterone (T) of male was $259.3{\pm}33.1pg/ml$ in the natural photoperiod, but the hightest value of $370.5{\pm}27.5pg/ml$ in 9L:15D. These results provide basic information from sexual maturation of spotted seabass.

• Journal of the Korean Wood Science and Technology
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• v.38 no.4
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• pp.333-340
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• 2010

The Korean Dendropanax lacquer, made from a natural resinous sap from Dendropanax orbifera Lev., was used as a golden and transparent varnish for the traditional artifacts (armor uits, helmets, arrowheads, etc.) to make them be brilliant golden color. The cured film of the acquer has excellent protective properties such as weatherability, water resistance, and nticorrosive. But, one of disadvantages is that takes a long time and much energy to fulfill curing the lacquer. The chemical constituents of the lacquer contained conjugated diene compounds s the photopolymerizable monomers. These monomers easily polymerized in sunlight to form olden-colored, hard-coating films in a short time. Photooxidation may be one of the most mportant reactions in the chemistry of the lacquer. Although the Korean Dendropanax Lacquer hould be dried to a thoroughly dry stage to achieve optimal film properties, curing with elevated emperatures may be required for the protracted curing time at atmospheric temperature. So we ntended to accelerate the curing rate of the lacquer by dual curing of thermal and radiation uring. The effect of thermal initiator on the thermal curing reaction was evaluated by monitoring he changes in double bond peak with FT-IR. Then the curing rate of the lacquer blended with hermal initiator and photoinitiator together was measured during dual curing using a RPT with V spot curing machine. Thermal initiator not only accelerated the curing rate but also improved he physical property. And the curing rate of the Korean Dendropanax lacquer was improved by ual curing method of thermal and UV curing. According to these results, the application area of he Korean Dendropanax lacquer could be expanded to surface coatings for electronic devices uch as mobile phones or electronics.

• Journal of the Korean Association of Geographic Information Studies
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• v.17 no.4
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• pp.69-85
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• 2014

Carbon stocks of NFI plots can be accurately estimated using field survey information. However, an accurate estimation of carbon stocks in other unsurveyed sites is very difficult. In order to fill this gap, various spatial information can be used as an ancillary data. In South Korea, there is the 1:5,000 forest type map that was produced by digital air-photo interpretation and field survey. Because this map contains very detailed forest information, it can be used as the high-quality spatial data for estimating carbon stocks. In this study, we compared three upscaling methods based on the 1:5,000 forest type map and 5th national forest inventory data. Map algebra(method 1), RK(Regression Kriging)(method 2), and GWR(Geographically Weighted Regression)(method 3) were applied to estimate forest carbon stock in Chungcheong-nam Do and Daejeon metropolitan city. The range of carbon stocks from method 2(1.39~138.80 tonC/ha) and method 3(1.28~149.98 tonC/ha) were more similar to that of previous method(1.56~156.40 tonC/ha) than that of method 1(0.00~93.37 tonC/ha). This result shows that RK and GWR considering spatial autocorrelation can show spatial heterogeneity of carbon stocks. We carried out paired t-test for carbon stock data using 186 sample points to assess estimation accuracy. As a result, the average carbon stocks of method 2 and field survey method were not significantly different at p=0.05 using paired t-test. And the result of method 2 showed the lowest RMSE. Therefore regression kriging method is useful to consider spatial variations of carbon stocks distribution in rugged terrain and complex forest stand.

• Journal of Dental Rehabilitation and Applied Science
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• v.28 no.3
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• pp.245-252
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• 2012

The purpose of this study was to evaluate the influence of glide path on canal centering ratio after instrumentation with different single file systems; WaveOne and Reciproc. Reciproc R25 (VDW), WaveOne Primary (Dentsply Maillefer) and PathFile #13, 16, 19 (Dentsply Maillefer) were used in this study. In no glide path groups, Reciproc files and WaveOne files used for canal preparation without glide path. In glide path groups, the PathFile were used before canal preparation. Methylene blue dye was introduced into the canal to obtain a clear pre-instrumentation image. Pre-instrumentation images and post-instrumentation images were scanned using Epson Perfection V700 Photo scanner (Epson, Nagano, Japan). Transparencies of post-instrumentation images were changed and superimposed on pre-instrumentation images using Adobe Photoshop CS 3 (Adobe Systems Incorporated, San Jose, CA, USA). The centering ratio was calculated for each instrumented canal using the following formula: CR=|X1-X2|/Y. It was statistically analyzed using two-way ANOVA at 95% confidential level. The centering ratio in glide path groups were significant less than it in no glide path groups at 3, 4, 5 and 6 mm level. Except 1 and 6 mm level, WaveOne groups had significant less centering ration than Reciproc groups. At 6 mm level, there was no significant difference between WaveOne and Reciproc. In the limitation of this study, creation of a previous glide path before reciprocating motion instrumentation in curved canal appears to be appropriate and WaveOne system can be used for preparation of curved canal without severe aberrations.

• Korean Chemical Engineering Research
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• v.52 no.3
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• pp.321-327
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• 2014

Polymer dispersed liquid crystal lenses of the cell gap of $11{\mu}m$ and $30{\mu}m$ were made from a uniformly dispersed mixture of 40 wt% NOA65 prepolymer - 60 wt% E7 liquid crystal with the variations of the additional photoinitiator. The photoinitiator, benzophenone of 5.0 wt% was originally in the commercial prepolymer NOA65. In this works, the influence of the benzophenone amount intentionally added in the commercial NOA65 on the electrical properties of polymer dispersed liquid crystal lens for smart electronic glasses. The additional quantities of the photoinitiator were 1, 2, 4, 8 and 16 wt% of the weight of NOA65 - E7 mixture. All the electro-optical properties of the sample with added benzophenone such as the driving voltage, the slope of the linear region, the response time and contrast ratio were more improved than that of commercial NOA65 only. These improvements were due to the increase of the average size of E7 liquid crystal droplets in the samples with the increase of the added benzophenon amount. The liquid crystal droplet size was increased from $5.3{\mu}m$ to $12.2{\mu}m$ when the photoinitiator was added from 0 wt% to 8 wt%. At the same concentration range of the photoinitiator, the driving voltage was ranged from 11.1 V to 17.3 V. The slopes of the linear region were in the range of 10.35~13.96 %T/V, which were more enhanced than that of NOA65 without the additional benzophenone. In particular, though the deteriorations by cell gap of $11{\mu}m$ were so effective to offset the influence of the added benzophenone for both rising and falling response time, it is confirmed that there were still somewhat improvement by the additional benzophenone. Response time and contrast ratios of all the samples with excess benzophenone were slightly enhanced.

• Korean journal of applied entomology
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• v.43 no.4 s.137
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• pp.297-304
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• 2004

The development of Aphidoletes aphidimyza, an aphidophagous gall midge, was studied at various constant temperatures ranging from 15 to $35^{\circ}C$, with $65{\pm}5\%$ RH, and a photo-period of 16L:8D. When A. aphidimyra was fed either on Aphis gossypii or Myzus persicae, it took 43.9 and 44.5 days, respectively, to develop from egg to pupa at $15^{\circ}C$, whereas at $25^{\circ}C$, 14.3 and 15.8 days. The developmental zero was 10.7 and $10.0^{\circ}C$, respectively, while the effective accumuative temperatures were 210.8 and 245.5 day-degrees. The nonlinear shape of temperature-dependent development, shown by A. aphidimyza when fed on either species of the aphids, was well described by the modified Sharpe and DeMichele model. When distribution model of completion time of development for each growth stage was expressed as physiological age and fitted to the Weibull fuction, the completion time of development gradually shortened from egg to larva, and to pupa. In addition, the coefficient of determination $r^2$ ranged between 0.86-0.93 and 0.85-0.94, respectively providing a good approximation of cumulative developmental rates. The life span of adult was 8.7 and 9.2 days at $15^{\circ}C$, and 3.1 and 2.7 days at $30^{\circ}C$, respectively. Egg incubation period was relatively short at $35^{\circ}C$ but hatchability was less than $50\%$ and the mortality of the larva at $35^{\circ}C$ reached $100\%$. At $30^{\circ}C$, the time of development lengthened and the adult longevity was short suggesting ill effect of high temperatures. Even though the life span of adults at $15^{\circ}C$ was relatively long, none moved freely in the rearing cage and no oviposition occurred. Accordingly, in case A. aphidimyza is adopted to suppress phytophagus aphid populations, it could be applicable to cropping systems with ambient temperatures above $20^{\circ}C$ and below $30^{\circ}C$. Within this range, A. aphidimyza adults was observed to be active and oviposit fully.

• The Journal of Korean Academy of Prosthodontics
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• v.52 no.2
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• pp.113-120
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• 2014

This study will evaluate the effectiveness of various pretreatments when fiber-reinforced composite (FRC) post is bonded to endodontically treated tooth with resin cement. Materials and methods: Canal shaping of FRC post (DT Light post, Size 3, Bisco Inc., Schaumburg, IL, USA) was performed on endodontically treated premolars at 1.5 cm from CEJ. Samples were divided into 6 groups of surface treatment after conventional washing and drying to the canal. Total of 24 FRC posts were randomly divided into 6 groups of surface treatment as follows: Group C: control - no surface treatment, Group A: airborne-particle abrasion (Cojet sand, 3M ESPE), Group S: silanization (Bis-silane, Bisco Inc.), Group M: universal primer (Monobond-plus primer, Ivoclar Vivadent Inc.), Group AS: silanization after airborne-particle abrasion, Group AM: universal primer treatment after airborne-particle abrasion. Pretreated fiber posts were cemented with resin-based luting material and photo-polymerized and cut to the thickness of 1 mm. Push-out test using a universal testing machine was performed. Bonding failure strength of post dislodgement was measured and the type of bonding failure was classified. Data were analyzed with Kruskal-Wallis test and multiple comparison groups were performed using Tukey HSD value of rank test (${\alpha}=0.05$). Results: Group AS showed significantly highest bonding strength. Group S, group AM, group A, and group M showed lower bonding strength in order. The control group showed the lowest bonding strength. Conclusion: Surface treatment with silane showed to be the most effective of the surface pretreatment methods for cementation of FRC post. Surface treatment with universal primer showed no significant difference compared with no surface treatment group as for bonding strength.