• Biomedical Science Letters
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• v.16 no.3
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• pp.187-192
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• 2010

The aim of this study was to compare the results of morphological phenotypic examination and molecular biological method, randomly amplified polymorphic DNA (RAPD) using clinical isolates from Korean patients infected with dermatophytes. A total of 44 clinical isolates have been collected by Korean Collection Medical Fungi (KCMF) and those were subjected for the RAPD analysis. The results showed that the fungal strains that have RG1 band patterns were identified as Trichophyton rubrum by the neighbour-joining analysis. Additionally, other fungal strains that have RG3 band patterns were identified as Epidermophyton floccosum and RG5 band patterns were identified as Micorsporum gypseum. Even though molecular biological method such as RAPD are not necessary, it might be useful when some strains have similar morphological characteristics or when same species has phenotypic variations. In this study, therefore, we targeted internal transcribed spacer (ITS) regions of fungal rDNA, performed RAPD and then compared with morphological phenotypic examination.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.1
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• pp.7-10
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• 2002

The breeding data relating to Hariana herd spread over 18 years (1979-96) were analysed to estimate genetic, phenotypic and environmental changes in characters of economic importance which might have taken place during the several years of selective breeding practiced in the herd. The average genetic changes in a given character were estimated by four methods. The phenotypic trends observed for different economic traits were not significant. On changing the method of estimation, magnitude and direction of genetic trends changed. Comparison of estimates of genetic trends by different methods showed that adjustments for biases due to non-random allotment of dams with respect to their age and merit suggested by Powell and Freeman (1974) were useful for increasing the precision of the estimates. Hence, this method was found to be the best method for estimation of genetic trends. The estimate of genetic trends by this method were 4.03${\pm}$6.21 days, 3.24${\pm}$5.33 kg, 0.15${\pm}$0.43 days, 0.09${\pm}$0.59 days, 0.01${\pm}$0.02 kg and 0.01${\pm}$0.01 kg for age at first calving, first lactation milk yield, first lactation length, first calving interval, first lactation milk yield per day lactation length and first lactation milk yield per day of calving interval, respectively.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.7
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• pp.931-936
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• 2009

Body weights of 862 Angora goats between birth and 36 months of age, recorded on a semiyearly basis from 1988 to 2000, were used to estimate genetic, permanent environmental and phenotypic covariance functions. These functions were estimated by fitting a random regression model with 6th order polynomial for direct additive genetic and animal permanent environmental effects and 4th and 5th order polynomial for maternal genetic and permanent environmental effects, respectively. A phenotypic covariance function was estimated by modelling overall animal and maternal effects. The results showed that the most variable coefficient was the intercept for both direct and maternal additive genetic effects. The direct additive genetic (co)variances increased with age and reached a maximum at about 30 months, whereas the maternal additive genetic (co)variances increased rapidly from birth and reached a maximum at weaning, and then decreased with age. Animal permanent environmental (co)variances increased with age from birth to 30 months with lower rate before 12 months and higher rate between 12 and 30 months. Maternal permanent environmental (co)variances changed little before 6 months but then increased slowly and reached a maximum at about 30 months. These results suggested that the contribution of maternal additive genetic and permanent environmental effects to growth variation differed from those of direct additive genetic and animal permanent environmental effects not only in expression time, but also in action magnitude. The phenotypic (co)variance estimates increased with age from birth to 36 months of age.

• Journal of Plant Biotechnology
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• v.43 no.1
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• pp.1-11
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• 2016

Single nucleotide polymorphism (SNP) is an abundant form of genetic variation within individuals of species. DNA polymorphism can arise throughout the whole genome at different frequencies in different species. SNP may cause phenotypic diversity among individuals, such as individuals with different color of plants or fruits, fruit size, ripening, flowering time adaptation, quality of crops, grain yields, or tolerance to various abiotic and biotic factors. SNP may result in changes in amino acids in the exon of a gene (asynonymous). SNP can also be silent (present in coding region but synonymous). It may simply occur in the noncoding regions without having any effect. SNP may influence the promoter activity for gene expression and finally produce functional protein through transcription. Therefore, the identification of functional SNP in genes and analysis of their effects on phenotype may lead to better understanding of their impact on gene function for varietal improvement. In this mini-review, we focused on evidences revealing the role of functional SNPs in genes and their phenotypic effects for the purpose of crop improvements.

• Archives of Pharmacal Research
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• v.29 no.2
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• pp.113-122
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• 2006

Among the effector molecules connected with the group of cell surface receptors, Ras proteins have essential roles in transducing extracellular signals to diverse intracellular events, by controlling the activities of multiple signaling pathways. For over 20 years since the discovery of Ras proteins, an enormous amount of knowledge has been accumulated as to how the proteins function in overlapping or distinct fashions. The signaling networks they regulate are very complex due to their multiple functions and cross-talks. Much attention has been paid to the pathological role of Ras in tumorigenesis. In particular, human tumors very frequently express Ras proteins constitutively activated by point mutations. Up to date, three members of the Ras family have been identified, namely H-Ras, K-Ras (A and B), and N-Ras. Although these Ras isoforms function in similar ways, many evidences also support the distinct molecular function of each Ras protein. This review summarizes differential functions of Ras and highlights the current view of the distinct signaling network regulated by each Ras for its contribution to the malignant phenotypic conversion of breast epithelial cells. Four issues are addressed in this review: (1) Ras proteins, (2) membrane localization of Ras, (3) effector molecules downstream of Ras, (4) Ras signaling in invasion. In spite of the accumulation of information on the differential functions of Ras, much more remains to be elucidated to understand the Ras-mediated molecular events of malignant phenotypic conversion of cells in a greater detail.

• Korean Journal of Ichthyology
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• v.12 no.2
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• pp.118-123
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• 2000

Heritabilities of growth traits, and phenotypic and genetic correlations for rearing masu salmon Oncorhynchus masou in adult stage were described. Genetic parameters were estimated for total length, body weight, and head length of masu salmon at 19 and 23 months of age. The heritabilities of growth traits estimated for sire component at 19 months of age was 0.22 in each trait in 1995, and ranged from 0.19 to 0.36 at 23 months of age in 1996. Phenotypic and genetic correlations were high among growth traits(0.85~0.98, 0.67~1.26, respectively). Family selection or that combined with individual selection is proposed to improve growth of masu salmon.

• Journal of Microbiology and Biotechnology
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• v.24 no.3
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• pp.401-407
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• 2014

Quorum sensing and the stringent response are well-known regulation systems for the expression of virulence genes in enterohemorrhagic Escherichia coli (EHEC). However, how these two systems interact is not well known. E. coli strains with mutations in two regulation systems, ${\Delta}luxS$ (ECM101) and ${\Delta}luxS{\Delta}relA{\Delta}spoT$ (ECM201), and the ${\Delta}luxS$ complement strain to ECM201 (ECM202) were created from EHEC O157:H7 EDL933 to investigate how the regulatory systems interact. The phenotypic changes of the mutant strains were characterized and compared with the wild type. The mutant strains exhibited no obvious growth defects, although acid resistance and cellular cytotoxicity were decreased significantly in all the mutant strains. Phenotypic characterization revealed that mutations in the stringent response system (ECM201 and ECM202) influenced the metabolic (defective utilization of arabinose and L-sorbose) and enzymatic activities (decreased trypsin activity, and increased ${\alpha}$-glucosidase activity). In contrast, the quorum sensing system mutant (ECM101) did not display these phenotypes. The motility of the quorum sensing system mutant (ECM101) was unchanged, but mutation in the stringent response system influenced the motility. Our results suggest that quorum sensing interacts with the stringent response regulation system.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2003.11a
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• pp.113-114
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• 2003

The observed ranges of genetic and phenotypic changes per generation were as follows, for the body weight at 150 days, -3.66 ∼ -8.99 and -27.07 ∼ -63.61 g ； for the body weight at 270 days, -2.89 ∼ -9.69 and 7.39 ∼ -27.45 g； for the age at first egg, -0.31 ∼ -0.57 and -0.23 ∼ -6.67 g ; for the egg weight at the first egg, -0.02 ∼ -0.06 and 0.65 ∼ 0.26 g ； for the egg weight at 270 days, 0.53 ∼ 0.35 and 1.62 ∼ 0.01, respectively. In general, the genetic and phenotypic change were decreased In body weight and the age at first egg and increased in egg weight and the number of egg production.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.276-276
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• 2022

Wheat is one of the most widely grown food crops worldwide. Extreme precipitation and wind disturbances increased due to the abnormal climate, which resulted in increased lodging. Introduction of dwarf genes in wheat significantly increased lodging resistance and productivity in wheat breeding. In this study, we performed the genotyping of dwarfing genes between 'Keumkang' and 'Komac 5' ('Keumkang' mutant). In addition, we investigated the relationship between plant height and several phenotypic characters using F₂ segregation populations derived from crosses between the two varieties. There was no significant difference in phenotypic characters between the two varieties except for plant height. In the genotyping analysis using dwarfing genes, mutations of two dwarfing gene were found to be induced between the two varieties. The four genotypes of the F₂ populations from a crossing between 'Keumkang' and 'Komac 5' were used to compare and evaluate the effects of two dwarfing genes. Plants with two single mutant dwarfing gene and double mutant dwarfing gene revealed reduced plant heights than control plants by 4.5%, 6.9%, and 33.2%, respectively. The phenotype analysis showed that double mutant dwarfing gene affected wheat stem growth as the length decreases from the second node, resulting in decreased plant height. However, there were no significant differences in the agronomic traits between mutant plants and control plant. These results may provide novel information about the effect of double mutant dwarfing gene on plant height, and may help improve lodging tolerance and wheat yield.

• Proceedings of the Korean Society of Crop Science Conference
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• 2020.06a
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• pp.120-120
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• 2020

Based on the simple sequence repeat (SSR) marker, a Korean wheat core collection were established with 616 wheat accessions. Among them, the SNP genotyping for the entire genome was performed using DNA chip array to clarify the whole genome SNP profiles. Consequently, a total of 35,143 SNPs were found and we re-established a mini-core collection with 247 accessions. Population diversity and phylogenetic analysis revealed genetic diversity and relationships from the mini core set. In addition, genome-wide association study (GWAS) was performed on 19 phenotypic traits; ear type, awn length, culm length, ear length, awn color, seed coat color, culm color, ear color, loading, leaf length, leaf width, seeding stand, cold damage, weight, auricle, plant type, heading stage, maturation period, upright habit, and degree of flag leaf. The GWAS was performed using the fixed and random model circulating probability unification (FarmCPU), which identified 14 to 258 SNP loci related to 19 phenotypic traits. Our study indicates that this Korean wheat mini-core collection is a set of germplasm useful for basic and applied research with the aim of understanding and exploiting the genetic diversity of Korean wheat varieties.