• Food Science and Preservation
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• v.11 no.3
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• pp.336-341
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• 2004

The influence of temperature and heating time on the stability of anthocyanin pigments in grape juice were investigated. There was no significant differences in soluble solids, acidity and pH of grape juice under various temperature and heating time. Residual total anthocyanin of grape juice was decreased by heating temperature over 100$^{\circ}C$ for 1 min. Delphinidin-3-glucoside and cyanidin-3-glucoside were rapidly decreased by heating treatment, but petunidin-3-glucoside and malvidin-3-glucoside were more stable than others. These results suggests that the optimum quality of grape juice was heated at 90 $^{\circ}C$ for 1 min.

• Food Science and Biotechnology
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• v.14 no.6
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• pp.778-782
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• 2005

Analysis of black soybeans [Glycine max (L.) Merr.; 59 Korean varieties] revealed that 100-seed weights of green cotyledon seeds (33.5 g, n=31) were higher than those of yellow ones (28.9 g, n=28). Contents of delphinidin-3-glucoside (D3G), cyanidin-3-glucoside (C3G), petunidin-3-glucoside (P3G), and total anthocyanins in seed coats of black soybeans were 0.03-4.15, 0.74-18.36, 0.02-1.60, and 0.87-23.52 mg/g, respectively, among which most prominent anthocyanin was C3G (80.9% of total content), followed by D3G (13.6%) and P3G (5.5%). No significant differences were observed in color parameters $a^*$ and $b^*$ between black soybeans with yellow cotyledon (BYC) and green cotyledon (BGC). Total isoflavone content of BGC was higher than that of BYC, and negative correlation was found between total anthocyanin and isoflavone contents.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.6
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• pp.512-515
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• 2004

The purpose of this experiment was to obtain informations about variation of anthocyanin content within black-seeded soybean testa according to cultivation and harvest time. Three varieties, Ilpumgeomjeongkong, Geomjeongkong 2, Tawonkong were tested in this experiment. Seeds were planted on April 15 (early planting), May 15 (optimum planting), and June 15 (late planting), respectively. Harvest time was R6 (full seed), R7 (physiological maturity), and R8 (harvest maturity), respectively. The content of delphinidin-3-glucoside (D3G), cyanidin-3-glucoside (C3G), petunidin-3-glucoside (Pt3G), and total anthocyanin (TA) had all increased gradually from April 15 to June 15 as well as R6 to R8. There was no varietal difference in D3G, but C3G and TA of Geomjeongkong 2 were higher than other two varieties. Pt3G was the highest Tawonkong of varieties. The value of L as anthocyanin color difference had gradually decreased from April 15 to June 15 and R6 to R8, Geomjeongkong 2 among three varieties was the lowest. The value of a was the highest on May 15 and increased gradually from R6 to R8, Geomjeongkong 2 was the lowest. The value of b was the highest on June 15 as late planting and was the highest Geomjeongkong 2 of varieties. The value of b had increased gradually from R6 to R8. D3G, C3G, Pt3G, and TA contents showed mutually high positive correlation and these four characters were represented high negative correlation with L value but showed high positive correlation with a and b values. The value of L showed high negative correlation with a and b values. The value of a showed high positive correlation with b value.

• Fashion & Textile Research Journal
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• v.9 no.1
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• pp.103-112
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• 2007

The purpose of this study was to investgate dyeing, moradnting properties and colorfastness activities of silk, polyamide, acetate treated black soybean extracts. The color of extract by buffer solution (pH=1) red, but the higher pH of extracted black soybean solution the paler of color strength. Black soybean extracts was approved anthocyanin of 3type(Delphinidin3-glucoside, Cyanidin 3-glucoside, Petunidin 3-glucoside) by chemiclal analysis. This anthocynin, in acid condition, is oxinium structure (red color, soluable) but, in alkali condition quinoid structure(dark brown, in soluable). The optimum dyeing condition of black soybean anthocynin was 10min at $30^{\circ}C$, pH -1. Colorfastness to washing and light showed generally low but crocking fastness was excellent.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2021.04a
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• pp.47-47
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• 2021

This study was carried out to obtain a basic information for the improvement of human health and the development of variety through analysis of organic compounds, contents of three CQA(3-caffeoylquinic acid, 4-caffeoylquinic acid and 5-caffeoylquinic acid) and five anthocyanin (petunidin-3-p-cumaroylrutinoside-5-glucoside, pelargonidin-3-p-cumaroylrutin-oside-5-glucoside, peonidin-3-p-cumaroylrutinoside-5-glucoside, pelargonidin-3-p-feruloyl-rutinoside-5-glucoside and peonidin-3-feruloylrutinoside-5-glucoside)to color potatoes is Hong-young(HY) and Ja-young(JY). The analytical results on organic compounds in color potatoes were shown as follow, The contents of CQA and Anthocyanin of JY variety were shown to be higher than HY, while CQA and Anthocyanin were appeared to be highest in peel of JY. Overall, JY had higher amount of physicochemical properties than HY. The results of this study reveal the quantitative analysis of functional compounds seperated from various kind of potatoes, which will enable the acquisition of new bioactive candidates and the establishment of new profit generation models for farmers.

• Food Science and Biotechnology
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• v.14 no.5
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• pp.672-675
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• 2005

Two anthocyanins were isolated from 1% HCl-20% methanol extracts of KG 97287 black common bean (Phaseolus vulgaris L.) using semipreparative, high-performance liquid chromatography (HPLC). The anthocyanins were identified using a combination of LC/ES-mass spectrometry (MS) and spectroscopic methods of UV-Vis, $^1H-$ and $^{13}C-$ nuclear magnetic resonance (NMR). The chemical structures of these two anthocyanins were elucidated as delphinidin 3-glucoside and petunidin 3-glucoside and their contents in KG 97287 black common bean seed coats were determined to be $2.614{\pm}0.11$ and $0.167{\pm}0.01\;mg/g$, respectively. These contents were lower than reported internationally and we recommend the introduction into Korea of high anthocyanin varieties of black common bean.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.1
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• pp.38-46
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• 2014

The purpose of this study was to investigate the effects of sowing time on the bio-active content of major soybean cultivars in a southern region of South Korea. Experiments were carried out in Naju, Jeonnam Province (latitude $35^{\circ}$ 04'N, longitude $126^{\circ}$ 54'E) from 2008 to 2010. The cultivars grown in summer included Saeol-kong and Tawon-kong and those grown in autumn included Taekwang-kong, Pungsannamul-kong, and Cheongja3, respectively. Sowing dates were May 15 and 30, June 15 and 30, and July 15 of each year. Daidzein content was increased in all the test cultivars grown from later sowing, and the contents of genistein and total isoflavone were increased in all the test cultivars except for Cheongja3. On the other hand, glycitein content showed no significant change in any of the test cultivars across the 5 sowing dates except for Tawon-kong. Cyanidin-3-glucoside (C3G) content was significantly increased in Tawon-kong and Cheongja3, and delphinidin-3-glucoside (D3G) and total anthocyanin contents were significantly increased in Tawon-kong. However, pelargonidin-3-glucoside (Pg3G) and petunidin-3-glucoside (Pt3G) contents showed no significant difference in plants sown across the 5 dates. Lutein content in Cheongja3 was between 2.7 and $3.0{\mu}g/g$ based on sowing date and did not vary significantly, while chlorophyll content increased with later sowing and was significantly higher in the cultivars sown on June 30 and July 15. Crude fat content was higher in Taekwang-kong and Cheongja3 when the cultivars were sown earlier; crude fat did not vary in the other test cultivars based on sowing date. Palmitic acid composition was not affected by sowing date in most of the test cultivars. Stearic acid was increased in Saeol-kong, Tawon-kong, and Cheongja3 with later sowing. Oleic acid and linoleic acid were significantly higher in Saeol-kong at an earlier sowing date. The composition of linolenic acid showed significantly increased dates in most of the test cultivars with later sowing. There were no significant differences in carbohydrate and crude protein content across 5 different sowing dates in most of the test cultivars. However, crude protein content was higher in Saeol-kong when sowing occurred earlier, and was higher in Tawon-kong at later sowing dates.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.6
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• pp.507-511
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• 2004

This research was carried out to offer the basic informations about new varietal breeding for specific use and physiological characteristics through investigation of detection, content variation and color difference of anthocyanin individual pigments within seed coats in domestic black soybean. The seed of thirteen cultivars such as Geomjeongkong 1, Geomjeongkong 2, Seonheugkong, Tawonkong, Ilpumgeomjeongkong, Geomjeongolkong, Cheongjakong, Jinju 1, Heugcheongkong, Juinunikong-Y, Juinunikong-G, Geomjeongkong 3, Geomjeongkong 4 was tested. C3G (cyanidin-3-glucoside) was detected in only Geomjeongkong 1 and Seonheugkong but D3G (delphinidin-3-glucoside) and C3G were found in Heugcheongkong. The rest cultivars that there were three anthocyanins such as D3G, C3G, and Pt3G (petunidin-3-glucoside). Anthocyanin content of tested cultivars showed a high variation. The ranges of D3G, C3G, Pt3G, and TA (total anthocyanin) contents were $0.55\~2.63mg/g,\;2.77\~8.38mg/g,\;0.38\~5.65mg/g,\;and\;3.32\~16.92mg/g$, respectively. These contents showed variation among cultivars as well as variation between two years, 2001-2002. As a result of variation of anthocyanin color difference, the ranges of L (lightness), a (redness), and b (yellowness) as Hunter's value were $34.09\~42.89,\;12.77\!22.85,\;and\;5.36\~12.10$, respectively, and these color differences showed variation among cultivars and also variation between two years, 2001-2002. D3G, C3G, Pt3G, and TA showed reciprocally a positive correlation being representive of high significance.

• Korean Journal of Plant Resources
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• v.34 no.6
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• pp.542-554
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• 2021

In this study, we cultivated 115 Soritae and 86 Yakkong black soybean landraces collected from ten different locations in Korea. Then, the variations of three yield components (one-hundred seeds weight (HSW), number of seeds per pod (SPP), and yield per plant (YPP)) and three anthocyanins (cyanidin-3-O-glucoside (C-3-O-G), delphinidin-3-O-glucoside (D-3-O-G) and petunidin-3-O-glucoside (Pt-3-O-G)) were investigated according to landrace type and collection area. Both yield components and anthocyanin contents significantly varied between the soybeans demonstrating genetic differences. Soritae landraces had the highest average HSW and TAC, whereas Yakkong landraces displayed the highest average SPP irrespective of collection area. Relatively, Yakkong landraces from Gyeongsangnam-do (1697.29 mg/100 g) and Soritae landraces from Gyeonggi-do (2340.94 mg/100 g) had the highest average TAC. Principal component analysis clearly separated Soritae and Yakkong landraces. Moreover, TAC and C-3-O-G showed positive and significant associations in both Soritae (r = 0.972) and Yakkong (r = 0.885) landraces, while yield components showed negative or weak correlations with each other. Overall, ten landraces were identified as important resources owing to their high yield (>150 g/plant) and high level of TAC (>2300 mg/100g). This study could lay foundations to molecular level investigations and reinforce the use of Yakkong and Soritae landraces during cultivar development.

• Food Science and Biotechnology
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• v.18 no.6
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• pp.1470-1475
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• 2009

The seed coat of the black soybean contains 3 main anthocyanins such as delphinidin-3-O-$\beta$-glucoside, cyanidin-3-O-$\beta$-glucoside, and petunidin-3-O-$\beta$-glucoside. As a part of our effort on discovering and breeding new black soybean cultivars which possesses specific anthocyanin component rich, we determined the anthocyanin profiles of the 2 cultivars recently developed soybean cv. Gaechuck #1 and cv. Gyeongsang #1, using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and compared their content and identity with those of previously known 10 cultivar controls. The Cosmosil-$5C_{18}$-AR-II column were selected for the analysis because of the best peak separation. The column temperature was set up at $35^{\circ}C$. The mobile phase consisting of water containing 0.5%(v/v) formic acid and methanol gave good separation between the 3 anthocyanin analytes and internal standard (quercetin 3-O-$\beta$-rutinoside) and peaks with suppressed tail. The MS/MS spectra of each individual anthocyanin standard were detected in positive electron spray ionization (ESI) modes. It was disclosed that the anthocyanin contents of the soybean cv. Gaechuck#1 and cv. Gyeongsang#1 are roughly higher than those of the 10 controls.