• The Journal of Korean Medicine
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• v.29 no.1
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• pp.200-206
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• 2008

Objective : To establish a standard for discriminating between deficiency syndrome and excess syndrome and selecting reinforcing or reducing method. Methods :Deficiency syndrome and excess syndrome were divided into excess of pathogenic factor with sufficiency of vital energy syndrome, weakness of pathogenic factor with deficiency of vital energy syndrome and excess of pathogenic factor with deficiency of vital energy syndrome. Documentary survey was done for each case. Results : Excess of pathogenic factor with sufficiency of vital energy syndrome is an excess syndrome and a reducing method must be used. Weakness of pathogenic factor with deficiency of vital energy syndrome is a deficiency syndrome and a reinforcing method must be used. Excess of pathogenic factor with deficiency of vital energy syndrome is related to deficiency syndrome and a reinforcing method must mainly be used. Conclusions :Deficiency or sufficiency of vital energy is the standard for discriminating between deficiency syndrome and excess syndrome and selecting a reinforcing or reducing method.

• Korean Journal of Food Science and Technology
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• v.30 no.6
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• pp.1432-1438
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• 1998

The gamma-radiation sensitivity of eight kinds of pathogenic bacteria related to beef was investigated in frozen cells $(-18^{\circ}C)$ with 0.1 M phosphate buffer and inoculated cells in beef. In frozen cells, D10 values of pathogenic bacteria related to beef were $0.07{\sim}0.69$ kGy, and inactivation factors were $2.90{\sim}42.86$ at the radiation doses of $2{\sim}3$ kGy. Beef was inoculated with Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, Enterobacter aerogenes, Vibrio parahaemolyticus, Staphylococcus aureus, Listeria monocytogenes, and Aeromonas hydrophila. Inoculated beef samples were packaged in air and irradiated at 0.005 to 3.0 kGy. Ninety percent of the viable pathogenic bacteria in beef was eliminated by doses of $0.1{\sim}0.61$ kGy at room temperature, and the inactivation factors were $3.28{\sim}30.0$ kGy at the radiation doses of $2{\sim}3$ kGy. Therefore, irradiation is considered to be an effective method to control pathogenic bacteria in beef.

• The Journal of Korean Medical History
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• v.15 no.1
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• pp.3-10
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• 2002

Acupuncture and moxibustion of theory had begun in the "Nei-Jing" and "Nan-Jing". But definite acupuncture needling techniques had been investigated after Jin and Yuan Dynasty. After Ming Dynasty, much more scholars had studied about definite acupuncture needling techniques. So various techniques and theorys about how to select and operate needling points appeared in print. "Zi-Wu-Liu-Zhu-Zhen-Jing", "Zhen-Jing-Zhi-Nan", "Shen-Ying-Jing", "Zhen-Jiu-Da-Quan" and so forth are representative books. But these books include mutually different techniques, so then scholars would be thrown into confusion. So I have analysed acupuncture and moxibustion of theory into Ki(vital energy)-blood's circulation, disease region, time, sex, restorative and restrictive techniques. As a result, I have known that every restorative and restrictive techniques and theories are connected with Ki(vital energy)-blood's circulation and practical application methods are decided by sex, time, disease region, pathogenic energy.

• Microbiology and Biotechnology Letters
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• v.46 no.3
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• pp.225-233
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• 2018

In this exploratory study, eight types of microalgae from different phyla (Chlamydomonas reinhardtii, Chlorella species, Haematococcus pluvialis, Porphyridium purpureum, Porphyridium cruentum, Isochrysis species, Isochrysis galbana, and Pavlova lutheri) were tested for their antibacterial activities against eight target pathogenic bacterial strains. The agar well diffusion method and broth micro dilution assay were conducted to estimate the antibacterial activity. Microalgae cell-free supernatants, exopolysaccharides (EPS), water, and organic solvent extracts were used for inhibition analysis. EPS extracted from P. lutheri showed activity against Bacillus subtilis and Pseudomonas aeruginosa. Inhibition zone diameters of 14-20 mm were recorded on agar plates, while the minimum inhibitory concentrations in the broth micro dilution assay were $0.39-25mg\;ml^{-1}$. During this study, haptophyte microalgae, Isochrysis species, and P. lutheri extracts showed the highest activity against most of the tested pathogenic bacterial strains, while most of the extracts were active against the important foodborne pathogen P. aeruginosa. This study showed promising results regarding important microalgae phyla, which will further aid research related to extracts and exploitation of bioactive metabolic compounds in the food and pharmaceutical industries.

• Korean Journal of Environmental Biology
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• v.22 no.4
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• pp.487-493
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• 2004

To investigate the antifungal activity related protein in pesticidal bacteria, a bacterial strain LTD was isolated from soil collected at Gimje in Jeonbuk province, Korea, and identified as Bacillus subtilis LTD based on a API50 CHB kit and 168 rDNA sequencing. It has an antifungal activity against 9 plant pathogenic fungi in a paper disc assay. The antifungal activity- deficient mutant, B. subtilis mLTD was induced at a 5 kGy dose of $^{60}Co$ gamma radiation. Using the two-dimensional electrophoresis and the matrix assisted laser desorption ionization time-of-flight mass spectrometry, the comparison analysis of proteins between the wild and mutant were performed. A major intracellular serine proteinase IspA (MW: 32.5 kDa), a NAD (P) H dehydrogenase (MW: 20.0 kDa), and a stage II sporulation protein AA, SpoIIAA (MW: 14.3kDa) were detected only in the B. subtilis LTD. These results suggested that the functions of these proteins found only in the B. subtilis LTD could. be closely related to the antifungal activity against plant pathogenic fungi.

• Food Science and Preservation
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• v.16 no.6
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• pp.965-970
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• 2009

PCR technology has been widely used to detect and quantify microbial pathogens in foodstuffs, because the technique is rapid, sensitive, and selective. In this study, detection of contaminating pathogenic bacteria on shells of chicken eggs was performed using both a commercial multiplex polymerase chain reaction (PCR) kit and a viable count method employing a selective medium. The PCR kit was capable of detecting Campylobacter jejuni, Escherichia coli O157:H7, Staphylococcus aureus, Bacillus cereus, Vibrio parahaemolyticus, Listeria monocytogenes, Yersinia enterocolitica, Salmonella species, and Shigella species. Using the PCR method, five bacterial species were detected from 30 samples (33.3%) of 90 batches of eggs commercially available in a market. PCR products from B. cereus, S. aureus, L. monocytogenes, Y. enterocolitica, and E. coli O157:H7 were detected, and the numbers and frequencies of positive samples were 17 (18.8%), 12 (13.3%), 15 (16.6%), 16 (17.7%),and 4 (4.4%), respectively. None of any Salmonella species, C. jejuni, V. parahaemolyticus, or Shigella species was detected in this study. The results of PCR testing were confirmed using a typical viable count method employing a selective medium. We suggest that the multiplex polymerase chain reaction (mPCR) assay is a rapid and reliable method for detection of pathogenic bacteria contaminating eggs.

• International journal of advanced smart convergence
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• v.4 no.1
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• pp.11-17
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• 2015

Fouling in heat exchanger becomes a major problem of dairy industry and it increases the production cost. These are lost productivity, additional energy, additional equipment, chemical, manpower, and environmental impact. Fouling also introduces the risk of food safety due to the improper heating temperature which allow the survival of pathogenic bacteria in milk, introducing biofilm formation of pathogenic bacteria in equipments and spreading the pathogenic bacteria to milk. The aim of this study is to determine the fouling rate during pasteurization process in heat exchanger of pasteurized milk produced by Village Cooperative Society (KUD) "X" in Malang, East Java Indonesia by using empirical modeling. The fouling rate is found as $0.3945^{\circ}C/h$ with the heating process time ranged from 0 to 2 hours and temperature difference (hot water inlet temperature and milk outlet temperature) ranged from 0.654 to $1.636^{\circ}C$. The fouling rate depends on type and characteristics of heat exchangers, time and temperature of process, milk type, age of milk, seasonal variations, the presence of microorganism and more. This results will be used to plan Cleaning In Place (CIP) and to design the control system of pasteurization process in order to maintain the milk outlet temperature as standard of pasteurization.

• Journal of Haehwa Medicine
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• v.16 no.1
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• pp.139-146
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• 2007

1. The etiological causes of Pain in Shoulder and Arm based on literatures of Oriental medicine are attack of wind-heat on the lung, wind cold, damp-heat struggle between the vital energy and pathogenic factor and six pathogenic factors And all these causes are the conception of blockage syndrome, $Q_1$ and blood stagnating in meridian system. 2. Symptoms of Pain in Shoulder and Arm based on literatures of Oriental medicine are shoulder pain, restriction of activity and radiating pain. 3. The treatments of Pain in Shoulder and Arm based on literatures of Oriental medicine are mainly composed of both medical therapy for $B_1$ syndrome due to pathogenic wind, deficiency of both $Q_1$ and blood, consumption of the liver and the spleen, and also acupuncture and moxibustion treatment by selection of acupoint. And those treatments are for treating etiology. And also there are treatments using the meridian system and Twelve Muscle Region and Ashihyeol for the purpose of treating the symptoms. 4. Acupoints such as Gyun-u, Gyun-jung, Goi-ji, Ju-ryo and Bi-no are most used in treating shoulder and arm pain based on based on literatures of Oriental medicine.

• The Journal of Korean Medicine
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• v.16 no.1 s.29
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• pp.251-270
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• 1995

In the Literatual Study on the Dysphonia, the results were as follows. 1. The causes of dysphonia are exogenous pathogenic factors,(specially cold evil)internal damage and meridian in The Yellow Emperor's Canon of Interal Medicine, since then endogenous pathogenic factors are lung-asthenia and deficiency of lung-yin etc. The main causes are disease caused by exogenous evils, general body weakness, emotional stimulation and excess of high voice rescently. 2. The pathogenesis of dysphonia originated from two factors; The first internal damages are consumption of body fluid with the formation of dryness evil resulting from the insufficienty of lung-yin and lung-collaterals damaged by heat-evil caused by deficiency of lung and kidney-yin. The second disease caused by exogenous evils is sluggishness of lung-energy caused by exogenous pathogenic factors. 3. The main relative organ are heart, lung and kidney etc. 4. The prescriptions of wind-cold symptoms are Samyoutang(三拗湯) and Hangsosan(杏蘇散), in the prescriptions of phlegm-heat symptom is Chenginyongphetang(淸咽寧肺湯), in the prescriptions of depressive syndrome due to disorder of vital energy are Sogangkitang(小降氣湯) and Shihochenggantang(柴胡淸肝湯加減), in the prescriptions of consumption of body fluid with the formation of dryness evil resulting from the insufficiency of lung-yin symptoms are Sanghangtang(桑杏湯) and Chenginguphetang(淸咽救肺湯, in the prescriptions of deficiency of lung and kidney-yin are Baekhabgokumtang(百合固金湯) and Maekmigiwhangtang(麥味地黃湯). 5. The treatment of acupunctures are used by LI-4(合谷), H-7(湧泉), Liv-3(太衝), K-3(太谿), Sp-6(三陰交), H-5(通里), GV-15(아門), CV-23(廉泉), S-40(農隆), K-6(照海), L-7(列缺), S-36(足三里) etc.

• Korean Journal of Acupuncture
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• v.20 no.2
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• pp.101-120
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• 2003

This study is performed to investigate the cause, symptom and acupuncture on Dudurugi through the literature of oriental medicine. The findings of this study are as follows: 1. Dudurugi is caused by exogenous pathogenic factors(wind, heat, cold, damp), sthenic inter damage factors(heat accumulated in the intestine and stomach, blood-heat, blood-stasia) and asthenia inter damage factors(asthenia of the spleen and stomach, blood-asthenia, asthenia of energy-blood, yin-asthenia and blood-dryness, yan-asthenia and energy-asthenia). 2. The symptom of Dudurugi is appeared in the skin and its surface is processed apparently or itch. 3. The treatment of Dudurugi was used by expelling the wind with removing pathogen. 4. In the treatment of Dudurugi, The su-yangmyong taejang-kyong of 12 merdians was mainly used and, the kokchi(LI11) of acupoints was most used in the acupuncture and moxibustion. 5. In the treatment of Dudurugi, acupoints of tok-maek and chok-t'aeyangkyong were mainly used in the case of showing symptoms caused by exogenous pathogenic factors and, acupoints of chok-t'aemkyong were mainly used in the case of showing symptoms caused by damp-heat accumulated in the intestine and stomach. When there were any other symptoms accompanied, other acupoints were more used.