• 한국식품위생안전성학회지
• /
• 제16권3호
• /
• pp.241-246
• /
• 2001

서울시를 비롯한 6개 도시의 음식점과 활어를 판매하는 어시장 등에서 활어조에 사용하는 해수에 대하여 병원성 비브리오균의 분포, 이화학적특성 및 위생지표세균 등 환경인자와의 관계를 비교하였다. 시험해수에서 전염병균인 Vibrio cholerae O1은 전혀 검출되지 않았고, V. parahaemolyticus는 가장 빈번한 검출되었는데 검출율은 69.2%이었다. 그리고 V. vulnificus 및 V. cholerae non-O1 등 병원성 비브리오균이 검출된 활어조의 해수는 그렇지 않은 해수에 비하여 수온과 탁도는 높았고, 염분과 pH는 낮았으며 생균수, 대장균군 및 분변계 대장균이 휠씬 높게 검출되어 이러한 환경인자들과의 관련성이 시사되었다.

• The Plant Pathology Journal
• /
• 제40권3호
• /
• pp.235-250
• /
• 2024

During the infection process, plant pathogenic fungi encounter plant-derived oxidative stress, and an appropriate response to this stress is crucial to their survival and establishment of the disease. Plant pathogenic fungi have evolved several mechanisms to eliminate oxidants from the external environment and maintain cellular redox homeostasis. When oxidative stress is perceived, various signaling transduction pathways are triggered and activate the downstream genes responsible for the oxidative stress response. Despite extensive research on antioxidant systems and their regulatory mechanisms in plant pathogenic fungi, the specific functions of individual antioxidants and their impacts on pathogenicity have not recently been systematically summarized. Therefore, our objective is to consolidate previous research on the antioxidant systems of plant pathogenic fungi. In this review, we explore the plant immune responses during fungal infection, with a focus on the generation and function of reactive oxygen species. Furthermore, we delve into the three antioxidant systems, summarizing their functions and regulatory mechanisms involved in oxidative stress response. This comprehensive review provides an integrated overview of the antioxidant mechanisms within plant pathogenic fungi, revealing how the oxidative stress response contributes to their pathogenicity.

• 한국응용곤충학회지
• /
• 제53권2호
• /
• pp.141-147
• /
• 2014

소나무재선충은 현재까지 도입된 국가의 토착수종인 소나무류를 모두 치사시키는 것으로 알려져 있으나, 고사목으로부터 추출 배양된 계통 중에는 소나무에 피해를 주지 않는 계통이 존재한다. 이러한 저병원성 계통 선충의 사전접종이 고병원성 소나무재선충에 대한 소나무의 저항성에 미치는 영향을 조사하기 위하여 연구가 수행되었다. 치수에 저병원성 소나무재선충을 선접종한 결과, 선접종에 의하여 소나무재선충에 대한 저항력이 유도되었다. 그 유도저항성은 1년 후까지 유지되었으며, 저병원성 선충의 반복 감염은 보다 저항성 유도 효과가 큰 것으로 나타났다. 소나무재선충에 의하여 고사한 후 경과기간이 3개월 이하인 피해목 및 피해진행목에서 분리된 선충은 모두 고병원성이었으며, 고사 후 2-3년이 경과한 벌채목에서 분리된 선충 5계통 중 4계통은 저병원성이었다. 저병원성 선충이 소나무 치수에 인공접종 되었을 때 초기 정착률은 고병원성 선충과 차이가 없었으나, 접종 30일 후에 수목내의 부위별 개체수를 조사한바 저병원성 선충의 증식률은 고병원성보다 현저히 낮았다. 저병원성 선충의 잿빛곰팡이병 균사배지에서의 선충증식 속도는 계통에 따라 차이가 있었으며, 증식속도가 빠른 계통은 대체로 고병원성 선충의 증식속도와 차이가 없었다.

• 한국응용곤충학회지
• /
• 제7권
• /
• pp.69-75
• /
• 1969

본 시험은 오이류 덩굴쪼김병균의 병원성을 조사하기 위하여 실시하였으며 공시균 24균주를 오이, 참외, 김치 참외, 멜론, 수박 및 수세미오이에 접종한 결과는 다음과 같다. 1. 수박분리균은 수박가 멜론에 대하여 강한 병원성을 나타냈고 그 중에는 김치참외 흑은 참외에 대해서도 병원성을 나타낸 것이 있었다. 2. 오이분리균은 병원성에 따라 네가지 형으로 나눌 수 있는데 오이에 대해서 강하게 병원성을 나타내면서 참외, 김치참외 및 멜론을 감염하는 것이 있었으며, 오이에 대해서는 병원성이 없거나 아주 약하게 나타난 것이 김치참외에 대해서는 강한 병원성을 나타낸 것이 있었다. 또 참외, 김치참외 및 멜론에 대해서만 강한 병원성을 나타내고 오이에 대해서는 전혀 병원성이 없는것과 오이, 참외, 김치참외, 멜론에 대해서는 강한 병원성을 나타내는 한편 수박에 대해서도 중간정도의 병원성을 나타내는 것이 있었다. 3. 참외 분리균은 병원성에 따라 네 가지 형으로 나눌 수 있는데 참외, 김치참외 및 멜론을 강하게 침해하는 것과 참외, 김치참외, 멜론은 물론 수박에 대해서도 병원성이 있는 것이 있었다. 또 김치참외 및 멜론에 대해서 병원성이 약하게 나타난 것과 멜론에 대해서는 강하게 병원성을 나타내고 수박을 약하게 침해하는 것이 있었다. 4. 오이류 덩굴쪼 병균은 병원성에 따라 수박덩굴 쪼김병균, 오이덩굴쪼김병균 및 멜론덩굴쪼김 병균으로 대별할 수 있었으나 공시돈 오이류 덩굴쪼김병균간의 병원성이나 기생범위가 반드시 일정하기 않기 때문에 몇 개의 form specine로 재정리하고 오이류에서 선발된 몇가지 판별품종에 대한 병원성에 따라 race로 나누어야 할 것이다.

• 대한한의학원전학회지
• /
• 제21권1호
• /
• pp.1-11
• /
• 2008

Jangjahwa(張子和) was influenced by "Hwangjenaegyeong(黃帝內經)" and Yuhagan(劉河間)'s theory, and other classics. Also, his clinical experience was helpful to theorize his thought. Thus, he improved medical theory by combining previous medical theory and his own experience. The essence of his thought is the importance of pathogenic Gi[邪氣] as the cause of disease and is Sambeop(三法) of Hantoha(汗吐下) as the methodology for removing pathogenic Gi[邪氣] away. He regarded pathogenic Gi as the cause of disease, and eliminated pathogenic Gi for the remedy. Namely, Sambeop(三法) of Hantoha(汗吐下) was selected as the best efficient method for driving pathogenic Gi away. Sambeop of Jangjahwa(張子和) have different meaning from previous one. Traditionally, Diaphoretic Therapy[汗法] was regarded as therapy for exogenous disease[外感病], and its effect was regarded as Balhanhaepyo(發汗解表). Emetic therapy[吐法] was throwing up Dameumsuksik(痰飮宿食) of stomach and above diaphragm. Purgation therapy[下法] means Tongbyeon(通便), Hajeok(下積), Sasil(瀉實), Chuksu(逐水) were regarded as therapy for Yangmyeongsiljeung(陽明實證) of Sanghan(傷寒). He submitted a new extensive concept of Sambeop adding traditional one, and expanded the application range of Sambeop. All methods, can cause circulation of Gihyeol(氣血) by opening the 'Hyeonbu(玄府)', like Moxibution therapy[灸薰], Steaming[蒸], Washing[洗],Heat therapy[慰], Cauterization[烙], Acupuncture therapy[鍼刺], Stone needling, Physical and breathing exercise[導引], Massage[按摩] were regarded as Diaphoretic Therapy[汗法]. Especially, he thought that Diaphoretic Therapy and venesection[瀉血] have same medical implication. If we examine the process of pushing out pathogenic Gi[邪氣] by means of Sambeop(三法), we can find the intermediation, that is circulation of Gihyeol(氣血). Its meaning is implied in the word of 'opening Hyeonbu(玄府)'. He thought that the circulation of Gihyeol(氣血) is the key to control health. Gihyeol(氣血) was circulated well under the physiological balance, but it was not circulated well under the invasion of pathogenic Gi[邪氣]. In other words, pathogenic Gi is the immediate cause of bad circulation of Gihyeol(氣血) and disease. Naturally, the doctor must remove pathogenic Gi that cause bad circulation for healing by means of Sambeop(三法). In my opinion, because the ultimate goal of Jangjahwa(張子和) was circulation of Gihyeol(氣血) by removing pathogenic Gi[邪氣], the concept of Sarnbeop(三法) could be expanded.

• 한국환경보건학회지
• /
• 제46권3호
• /
• pp.353-358
• /
• 2020

Objectives: An outbreak of pathogenic Escherichia coli food poisoning in Korea was first reported in 1998. They have continued to occure since then. This study was performed to describe the long-term trend in pathogenic E. coli food poisoning occurrences in Korea and examine the relation with climate factors. Methods: Official Korean statistics on food poisoning outbreaks and meteorological data for the period 2002-2017 were used. Pearson's correlation analysis was employed to establish the relationship between outbreaks of pathogenic E. coli food poisoning and meteorological factors. The influence of meteorological factors upon the outbreaks was analyzed by regression analysis. Results: During the study period, pathogenic E. coli food poisoning ranked second for the number of outbreaks (excluding unknowns) and first for the number of cases. Average temperature, the highest and lowest temperatures, precipitation, number of days with rainfall, and humidity all had a significant correlation with monthly number of outbreaks of pathogenic E. coli food poisoning (p<0.001). It was found that the lowest and highest temperatures and precipitation had a significant influence on the monthly number of outbreaks of food poisoning (p<0.001). These variables together explained 42.1% of the total variance, with the lowest temperature having the greatest explanatory power. Conclusion: These results show that food poisoning incidences may have been influenced by climate change, especially warming. The results also suggest that pathogenic E. coli infections are now an important public health issue in Korea since it is one of the countries where climate change is occurring rapidly.

• Food Science and Biotechnology
• /
• 제18권5호
• /
• pp.1243-1252
• /
• 2009

User-friendly software was developed to determine the shelf-life of perishable Korean seasoned side dishes in real time based on growth models of spoilage and pathogenic microorganisms. In the program algorithm, the primary spoilage and fastest-growing pathogenic organisms are selected according to the product characteristics, and their growth is simulated based on the previously monitored or recorded temperature history. To predict the growth of spoilage organisms with confidence limits, kinetic models for aerobic bacteria or molds/yeasts from published works are used. Growth models of pathogenic bacteria were obtained from the literature or derived with regression of their growth rate data estimated from established software packages. These models are also used to check whether the risk of pathogenic bacterial growth exceeds that of food spoilage organisms. Many example simulations showed that the shelf-lives of the examined foods are predominantly limited by the growth of spoilage organism rather than by pathogenic bacterial growth.

• Journal of Dairy Science and Biotechnology
• /
• 제39권2호
• /
• pp.68-77
• /
• 2021

This study aimed to evaluate the effects of antibacterial activity of Tibetan mushroom exopolysaccharide against foodborne pathogenic bacteria Staphylococcus aureus 305, Listeria monocytogenes ATCC19114, Escherichia coli O157:H7 ATCC42894 and Escherichia coli O55. The yield of exopolysaccharide isolated from Tibetan mushroom culture was 620 mg/L. The antibacterial activity of exopolysaccharide against foodborne pathogenic bacteria exhibited 15 mm and 12 mm clear zone against S. aureus 305 and L. monocytogenes ATCC 19114, respectively. However, no clear zone was observed against E. coli O157:H7 ATCC 42894 and E. coli O55. In conclusion, exopolysaccharide isolated from Tibetan mushroom culture have the antibacterial activity only against Gram-positive foodborne pathogenic bacteria.

• 대한한의학원전학회지
• /
• 제19권3호
• /
• pp.421-426
• /
• 2006

Objective To learn if there were different characteristics of tongue demonstration between diseases caused by exogenous pathogenic factor and disorder of internal organs. Methods Choosing 3059 cases from 51 medical records of Qing Dynasty, in which 1397 cases were diagnosed as diseases caused by exogenous pathogenic factor and 1110 were disorder of internal organs. Contrasting the constituent ratio of tongue demonstration using chi-square test. Results There were significant deviation between diseases caused by exogenous pathogenic factor and disorder of internal organs in color of tongue, color of fur and quality of fur (p<0.01). Conclusions the characteristics of tongue demonstration in diseases caused by exogenous pathogenic factor were high frequency of bright red tongue, yellow fur, blank fur and dry fur; and which in disorder of internal organs were high frequency of white tongue, thin fur, greasy fur and exfoliative fur.

• Journal of Microbiology and Biotechnology
• /
• 제17권7호
• /
• pp.1191-1197
• /
• 2007

The detection and kinetics of mucosal pathogenic bacteria binding on polysaccharide ligands were studied using a surface plasmon resonance biosensor. The kinetic model applied curve-fitting to the experimental surface plasmon resonance sensorgrams to evaluate the binding interactions. The kinetic parameters for the mucosal pathogenic bacteria (Pseudomonas aeruginosa, Pseudomonas fluorescens, Serratia marcescens) with the alginate ligand were determined from a kinetic model. In addition, the binding interactions of the mucosal pathogenic bacteria with polysaccharide binding pairs (Pseudomonas aeruginosa/alginate, Streptococcus pneumoniae/pneumococcal polysaccharide, Staphylococcus aureus/pectin) were also compared with their kinetic parameters. The rate constants of association for Pseudomonas aeruginosa with the alginate ligand were higher than those for Pseudomonas fluorescens. Serratia marcescens had no detectable interaction with the alginate ligand. The adhesion affinity of Pseudomonas aeruginosa with alginate was higher than that for the other binding pairs. The binding affinities of the pathogenic bacteria with their own polysaccharide were higher than that of Staphylococcus aureus with pectin. Measuring the contact angle was found to be a feasible method for detecting binding interactions between analytes and ligands.