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http://dx.doi.org/10.5656/KSAE.2014.03.0.013

Low-pathogenic Pinewood Nematode Found in Dead Trees and Resistance of Pines Induced by Its Pre-inoculation  

Park, Seung-Chan (Department of Forest Resources, Chonnam National University)
Moon, Yil-Sung (Forestry Research Institute)
Kim, Dong-Soo (Forestry Research Institute)
Publication Information
Korean journal of applied entomology / v.53, no.2, 2014 , pp. 141-147 More about this Journal
Abstract
Pinewood nematode (PWN: Bursaphelenchus xylophilus) is known to kill pine tree species that are indigenous to countries where the pest was inadvertently imported, but some cultures from the extraction of dead pines do not damage trees. Experiments were conducted to examine the effect of pre-inoculation of these low-pathogenic pinewood nematode on resistance of pine trees against the pest species. The pre-inoculated pine saplings showed induced resistance which lasted for a year, and repeated inoculation of these low-pathogenic nematodes enhanced tree resistance. All nematode samples extracted from dying or dead pines that had been killed not more than three months before the extraction were pathogenic, and most of those extracted from pines that had been killed 2-3 years before were low-pathogenic. When inoculated in pine saplings, number of low-pathogenic nematodes settled, as studied two days after inoculation, was not different from that of pathogenic ones. However, as studied after 30 days of inoculation, rate of reproduction in low-pathogenic nematodes was far lower than that of pathogenic nematodes. The rate of reproduction of several nematode isolates growing on fungal mat media of Botrytis cinerea varied, but three of four low-pathogenic isolates showed same level of reproduction rates as pathogenic ones.
Keywords
Pinewood nematode; Bursaphelenchus xylophilus; Low-pathogenic; Pre-inoculation; Induced resistance;
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