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Effects of parental folate deficiency on the folate content, global DNA methylation, and expressions of FR${\alpha}$, IGF-2 and IGF-1R in the postnatal rat liver

  • Mejos, Karen Kay;Kim, Hye Won;Lim, Eun Mi;Chang, Namsoo
    • Nutrition Research and Practice
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    • v.7 no.4
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    • pp.281-286
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    • 2013
  • We examined the effect of parental folate deficiency on the folate content, global DNA methylation, folate receptor-alpha (FR${\alpha}$), insulin-like-growth factor-2 (IGF-2) and -1 receptor (IGF-1R) in the liver and plasma homocysteine in the postnatal rat. Male and female rats were randomly fed a folic acid-deficient (paternal folate-deficient, PD and maternal folate-deficient, MD), or folic acid-supplemented diet (paternal folate-supplemented, PS and maternal-folate-supplemented, MS) for four weeks. They were mated and grouped accordingly: $PS{\times}MS$, $PS{\times}MD$, $PD{\times}MS$, and $PD{\times}MD$. Pups were killed on day 21 of lactation. The hepatic folate content was markedly reduced in the $PD{\times}MD$ and $PS{\times}MD$ and $PD{\times}MS$ as compared with the $PS{\times}MS$ group. The hepatic global DNA methylation was decreased in the $PD{\times}MS$ and $PS{\times}MD$ groups as much as in the $PD{\times}MD$ group, and all the three groups were significantly lower as compared to the $PS{\times}MS$ group. There were no significant differences in the hepatic FR${\alpha}$, IGF-2 and IGF-1R expressions among the groups. Positive correlations were found between the hepatic folate content and global DNA methylation and protein expressions of FR${\alpha}$, IGF-2 and IGF-1R, whereas an inverse correlation was found between hepatic folate content and plasma homocysteine level in the 3-week-old rat pup. The results of this study show that both paternal and maternal folate deficiency at mating can influence the folate content and global DNA methylation in the postnatal rat liver.

The Effect of Paternal and Maternal Helicopter Parenting on the Career Preparation Behavior of High-School Students: Sequential Mediation Effects of Academic Achievement Attribution and Career Decision-Making Self-Efficacy (아버지와 어머니의 헬리콥터 부모역할이 고등학생의 진로준비 행동에 미치는 영향: 학업성패귀인과 진로결정자기효능감의 순차적 매개효과)

  • Yoon Seo Kim;Min Ju Kang
    • Human Ecology Research
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    • v.61 no.3
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    • pp.401-414
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    • 2023
  • This study examined the sequential mediation effects of academic achievement attribution and career decision-making self-efficacy on the effect of paternal and maternal helicopter parenting on high-school students' career preparation behavior. A total of 285 (119 male and 166 female) Korean high-school students in the second grade participated in the study. Research variables were measured using the Career Preparation Behavior Scale (Kim, 1997), Helicopter Parenting Scale (LeMoyne & Buchanan, 2011), Attribution Questionnaire (Weiner, 1979), and Career Decision-Making Self-Efficacy Scale-Short Form (Betz et al., 1996). To examine the sequential mediating effect, data analysis was performed using SPSS 29.0 and PROCESS MACRO (v4.2) Model 6. The results revealed no correlations between helicopter parenting and academic failure attribution. However, higher paternal and maternal helicopter parenting were found to indirectly reduce high-school students' career preparation behavior through lower internal academic success attribution (effort and ability) and higher external academic success attribution (task difficulty and luck), which reduced career decision-making self-efficacy. These findings can be employed to develop more effective intervention programs comprising career guidance for adolescents, which emphasizes the negative effect of helicopter parenting. This study expands the research field, as previous findings on helicopter parenting mostly focus on college students.

Effect of Adolescents' Perceived Parental Blame on Learned Helplessness: The Sequential Mediating Effects of Maladaptive Metacognitive Beliefs and Rumination (청소년이 지각한 부모의 비난이 학습된 무기력에 미치는 영향에서 역기능적 메타인지신념과 반추의 순차적 매개효과)

  • Jiyoon Kang;Min Ju Kang
    • Human Ecology Research
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    • v.62 no.1
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    • pp.101-120
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    • 2024
  • This study aimed to examine the effect of adolescents' perceived parental blame (criticism) on learned helplessness and to examine whether maladaptive metacognitive beliefs and rumination sequentially mediate the relationship between parental blame and learned helplessness. The participants were 316 adolescents (Mean age=16.7, SD=0.75; 137 male, 179 female) attending grades 1st and 2nd in high school in South Korea. The participants were selected using a snowball sampling method, while the data was collected via an online self-report questionnaire. This survey was completed by the participants and analyzed using SPSS 28.0, Amos 26.0 (IBM Co., Armonk, NY), and PROCESS macro version 4.2 (Model 6; Hayes, 2022). The main results are summarized as follows. Firstly, the adolescents' perceived paternal and maternal blame indicated significant direct effects on learned helplessness. Secondly, rumination mediated the effect of paternal and maternal blame on learned helplessness. Lastly, paternal and maternal blame significantly affected learned helplessness through the sequential mediating effects of maladaptive metacognitive beliefs and rumination. This study elucidates the causal structure among the various factors influencing learned helplessness in adolescents, focusing on parental blame, maladaptive metacognitive beliefs, and rumination. Furthermore, considering the verified sequential mediating effects of maladaptive metacognitive beliefs and rumination in the relationship between adolescents' perceived parental blame and learned helplessness, these findings suggest that modifying maladaptive metacognitive beliefs may help to reduce learned helplessness among adolescents who perceive high levels of parental blame.

A Study on Effects of Breeding Combination for Feeding and Economic Analysis in Broiler Stock (육용종계의 교배조합이 실용계의 사양과 경제성에 미치는 영향)

  • 박준영;오세정
    • Korean Journal of Poultry Science
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    • v.7 no.1
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    • pp.31-42
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    • 1980
  • In order to identify the best superior cross combination of breeder stocks for broiler production, combining ability test and analyses of phenotypic performances for parent stocks were examined on records of 1,440 broiler chicken which were produced from 4 parental strains and 3 maternal strains at Hanhyup Poultry Breeding Farm from September 28, 1978 to January 5, 1979. The results obtained were as follows; 1. There was not found heterosis effect in viability but it seems to be desirable to select Hubbard strain in paternal line to improve viability. 2. As the paternal and maternal lines, selection of Ross strain showed the best paternal and maternal performance and the best general combining ability in body weight at 8 weeks of age is expected to be able to improve body weight of it s crossbred And the most superior cross combinations based on the specific combining ability and performance of rack crossbred were identified as Hubbard x Ross ana Ross x Hypeco crossbreds. 3. The best paternal and maternal lines on the smallest feed consumption for 8 weeks were Hubbard and Ross strains, and Hypeco strain, respectively. Especially Hubbard x Hypeco cross combination was proved as the smallest feed consumption compared with other cross combinations. 4. In feed requirement per Kg body weight increase, Hubbard strain for paternal line, Hypeco strain for naternal line, and cross combinations of Hubbard x Hypeco, Hubbard x Ross and Ross x Hypeco were certified as the most superiors. 5. Also superior cross combinations of Hubbard x Hypeco and Hubbard x Ross earned the most profit per bird through economic analysis. According to results as shown above, this experiment seems to be able to reach a such conclusion that production of superior cross combinations Hubbard x Ross, Hubbard x Hypeco and Ross x Hypeco through selection of Ross and Hubbard strains to paternal line and Hypeco and Ross strains for maternal line may become to considerable improvement for important economic characters of broiler; viability, body weight, feed consumption and feed requirement.

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Identification of True Full Sib Progenies of Japanese Red Pine via cpSSR Haplotyping (cpSSR haplotype에 근거한 소나무 전형매차대목(全兄妹次代木) 검정(檢定))

  • Hong, Yong-Pyo;Kwon, Hae-Yun;Han, Sang-Urk;Choi, Wan-Yong;Kim, Yong-Yul
    • Journal of Korean Society of Forest Science
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    • v.94 no.3 s.160
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    • pp.178-182
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    • 2005
  • To identify the seedlings from controlled pollination between one paternal tree and three maternal trees of Japanese red pine, cpSSR markers of the paternally inherited haploid genome were analyzed in two year old 114 seedlings of full sib families. Individual specific DNA fingerprint like haplotypes of the parental trees were determined by PCR with three cpSSR primers. Haplotypes of the 114 seedlings were also identified by PCR with the same primers. On the basis of the comparison of cpDNA haplotypes of the 114 seedlings with those of the parental trees, 14 seedlings revealed to have distinguished haplotypes from those of the paternal tree. It was tentatively concluded that they were generated via pollination with the non-paternal trees. A seedling of Gangwon30 revealing non-paternal haplotype might have been generated via self pollination with the pollens of maternal tree through improper emasculation or contamination during artificial pollination. DNA fingerprint like cpSSR profiles observed in this study could be successfully applied to the various plant forensic analyses, such as identification of siblings of individual trees, asexually reproduced ramets of a specific clone, vegetatively propagated individuals via tissue culture, and pure full sib progenies.

Paternal Childcare Time for Preschool Children and Its Determinants on Working and Nonworking Days (미취학자녀를 둔 아버지의 근무일과 비근무일의 자녀돌봄시간과 영향 요인 - 맞벌이 여부 및 돌봄유형별 차이를 중심으로 -)

  • Kim, Yookyung
    • Journal of Family Resource Management and Policy Review
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    • v.26 no.2
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    • pp.71-84
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    • 2022
  • This study analyzed 2019 time survey data from the National Statistical Office to examine the childcare behaviors of fathers with preschool children and their determinants, considering types of care and working/nonworking days. The main results of this study are as follows. First, paternal childcare time of nonworking days was three times more than that of working days, and the participation rate was also higher on nonworking days. Second, there was no significant difference in the amount of time spent on primary care and developmental care by fathers, whether from dual- or single-income families. Third, it seems that fathers adjust their participation in childcare between working days and nonworking days in consideration of the mother's time availability. Fourth, the variables related to childcare needs had a significant influence on paternal childcare time on both working and nonworking days. Fathers' developmental care time was not explained by the independent variables entered into the regression analysis. As a result of the study, it is necessary to reduce fathers' working hours and increase family-friendly systems to increase fathers' participation in childrearing. Fathers' perception of parental responsibility must also be changed.

An Exploratory Study of Korean Fathering I : Paternal Involvement and Children's Sex Role Orientation (아버지의 역할수행에 관한 탐색적 연구 I : 아버지의 역할참여와 아동의 성역할 지향)

  • Yang, Jang Ae
    • Korean Journal of Child Studies
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    • v.20 no.1
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    • pp.135-145
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    • 1999
  • Little is known about the relationship between fathers and their preadolescent children in Korea or about variations in fathering by SES and sex role orientation. The purpose of this exploratory research was to examine variation in contemporary Korean fathering (e.g., warmth of fathering, frequency of father involvement, and task share of father involvement) and its influence on children's sex role development. One hundred and twenty-nine fathers, mothers, and their 11-13-year-old children completed standardized survey questionnaires addressing their childrearing practices, parental role involvement, spousal support, and sex role orientations. Data were analyzed using MANOVAs, t-test, correlational analyses, and multiple regression analyses. Mothers reported more warmth in parenting than did fathers. Regardless of sex of child middle class fathers reported more warmth than lower class fathers and lower frequency of task share than lower class fathers. Regardless of SES, fathers with working wives reported higher levels of task share of involvement relative to their spouse. Fathers who were more frequently involved with their children tended to receive greater support from their wives for paternal involvement. There were no differences in parenting by sex of child nor was fathering associated with children's sex role orientation. Girls' femininity was related to fathers' masculinity. SES, maternal support, fathers' femininity, parents' education level, and maternal work status had predictive ability for the ecological view that fathering is a dynamic process predicted by personal characteristics as well as contextual factors.

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Relationships among Behavior Problem, Peer Interaction, and Parental Factors in Young Boys and Girls with Atopic Dermatitis (유아의 성별 문제행동과 또래상호작용 및 부모 요인과의 관계: 아토피피부염 유아를 중심으로)

  • Chun, Hui Young
    • Korean Journal of Childcare and Education
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    • v.12 no.6
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    • pp.19-39
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    • 2016
  • The present study examined differences in children's behavior problems and peer interaction, and parental factors including self-esteem, depression and childrearing stress between atopic dermatitis(AD) and normal(non-AD) children in each gender, and analyzed the effects of the parental factors on the two variables of AD children. 165 AD and 1176 non-AD children of age 5 were selected from the 6th year data of the Panel Study on Korean Children. The results from t-test and multiple regression are as follows. First, only AD boys showed a higher level of behavior problems than non-AD boys, but peer interaction showed no difference between AD and non-AD groups. Compared with non-AD children's parents, mothers of AD children showed a higher stress level and mothers of AD boys had a higher depression level. But there were no differences in maternal self-esteem and paternal factors between the groups. Second, AD boys' behavior problems were related to maternal factors and paternal stress, and some characteristics of AD girls' behavior problems were related with some parental variables. But peer interaction of AD children had no relation with parental factors. Third, behavior problems were influenced by maternal stress in AD boys and influenced by maternal depression and parental self-esteem in AD girls.

A Least Squares Regression Model to Detect Quantitative Trait Loci with Polar Overdominance in a Cross of Outbred Breeds: Simulation

  • Kim, Jong-Joo;Dekkers, Jack C.M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.26 no.11
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    • pp.1536-1544
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    • 2013
  • A least squares regression interval mapping model was derived to detect quantitative trait loci (QTL) with a unique mode of genomic imprinting, polar overdominance (POD), under a breed cross design model in outbred mammals. Tests to differentiate POD QTL from Mendelian, paternal or maternal expression QTL were also developed. To evaluate the power of the POD models and to determine the ability to differentiate POD from non-POD QTL, phenotypic data, marker data and a biallelic QTL were simulated on 512 F2 offspring. When tests for Mendelian versus parent-of-origin expression were performed, most POD QTL were classified as partially imprinted QTL. The application of the series of POD tests showed that more than 90% and 80% of medium and small POD QTL were declared as POD type. However, when breed-origin alleles were segregating in the grand parental breeds, the proportion of declared POD QTL decreased, which was more pronounced in a mating design with a small number of parents ($F_0$ and $F_1$). Non-POD QTL, i.e. with Mendelian or parent-of-origin expression (complete imprinting) inheritance, were well classified (>90%) as non-POD QTL, except for QTL with small effects and paternal or maternal expression in the design with a small number of parents, for which spurious POD QTL were declared.