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Identification of True Full Sib Progenies of Japanese Red Pine via cpSSR Haplotyping  

Hong, Yong-Pyo (Division of Forest Genetic Resources, Korea Forestry Research Institute)
Kwon, Hae-Yun (Division of Forest Genetic Resources, Korea Forestry Research Institute)
Han, Sang-Urk (Division of Forest Genetic Resources, Korea Forestry Research Institute)
Choi, Wan-Yong (Division of Forest Genetic Resources, Korea Forestry Research Institute)
Kim, Yong-Yul (Division of Forest Genetic Resources, Korea Forestry Research Institute)
Publication Information
Journal of Korean Society of Forest Science / v.94, no.3, 2005 , pp. 178-182 More about this Journal
Abstract
To identify the seedlings from controlled pollination between one paternal tree and three maternal trees of Japanese red pine, cpSSR markers of the paternally inherited haploid genome were analyzed in two year old 114 seedlings of full sib families. Individual specific DNA fingerprint like haplotypes of the parental trees were determined by PCR with three cpSSR primers. Haplotypes of the 114 seedlings were also identified by PCR with the same primers. On the basis of the comparison of cpDNA haplotypes of the 114 seedlings with those of the parental trees, 14 seedlings revealed to have distinguished haplotypes from those of the paternal tree. It was tentatively concluded that they were generated via pollination with the non-paternal trees. A seedling of Gangwon30 revealing non-paternal haplotype might have been generated via self pollination with the pollens of maternal tree through improper emasculation or contamination during artificial pollination. DNA fingerprint like cpSSR profiles observed in this study could be successfully applied to the various plant forensic analyses, such as identification of siblings of individual trees, asexually reproduced ramets of a specific clone, vegetatively propagated individuals via tissue culture, and pure full sib progenies.
Keywords
Japanese red pine; artificial pollination; full sib progenies; cpSSR; haplotyping;
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