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Metagenome-Assembled Genomes of Komagataeibacter from Kombucha Exposed to Mars-Like Conditions Reveal the Secrets in Tolerating Extraterrestrial Stresses

  • Lee, Imchang;Podolich, Olga;Brenig, Bertram;Tiwari, Sandeep;Azevedo, Vasco;de Carvalho, Daniel Santana;Uetanabaro, Ana Paula Trovatti;Goes-Neto, Aristoteles;Alzahrani, Khalid J.;Reva, Oleg;Kozyrovska, Natalia;de Vera, Jean-Pierre;Barh, Debmalya;Kim, Bong-Soo
    • Journal of Microbiology and Biotechnology
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    • v.32 no.8
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    • pp.967-975
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    • 2022
  • Kombucha mutualistic community (KMC) is composed by acetic acid bacteria and yeasts, producing fermented tea with health benefits. As part of the BIOlogy and Mars EXperiment (BIOMEX) project, the effect of Mars-like conditions on the KMC was analyzed. Here, we analyzed metagenome-assembled genomes (MAGs) of the Komagataeibacter, which is a predominant genus in KMC, to understand their roles in the KMC after exposure to Mars-like conditions (outside the International Space Station) based on functional genetic elements. We constructed three MAGs: K. hansenii, K. rhaeticus, and K. oboediens. Our results showed that (i) K. oboediens MAG functionally more complex than K. hansenii, (ii) K. hansenii is a keystone in KMCs with specific functional features to tolerate extreme stress, and (iii) genes related to the PPDK, betaine biosynthesis, polyamines biosynthesis, sulfate-sulfur assimilation pathway as well as type II toxin-antitoxin (TA) system, quorum sensing (QS) system, and cellulose production could play important roles in the resilience of KMC after exposure to Mars-like stress. Our findings show the potential mechanisms through which Komagataeibacter tolerates the extraterrestrial stress and will help to understand minimal microbial composition of KMC for space travelers.

Analysis of Distributed Computational Loads in Large-scale AC/DC Power System using Real-Time EMT Simulation (대규모 AC/DC 전력 시스템 실시간 EMP 시뮬레이션의 부하 분산 연구)

  • In Kwon, Park;Yi, Zhong Hu;Yi, Zhang;Hyun Keun, Ku;Yong Han, Kwon
    • KEPCO Journal on Electric Power and Energy
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    • v.8 no.2
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    • pp.159-179
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    • 2022
  • Often a network becomes complex, and multiple entities would get in charge of managing part of the whole network. An example is a utility grid. While the entire grid would go under a single utility company's responsibility, the network is often split into multiple subsections. Subsequently, each subsection would be given as the responsibility area to the corresponding sub-organization in the utility company. The issue of how to make subsystems of adequate size and minimum number of interconnections between subsystems becomes more critical, especially in real-time simulations. Because the computation capability limit of a single computation unit, regardless of whether it is a high-speed conventional CPU core or an FPGA computational engine, it comes with a maximum limit that can be completed within a given amount of execution time. The issue becomes worsened in real time simulation, in which the computation needs to be in precise synchronization with the real-world clock. When the subject of the computation allows for a longer execution time, i.e., a larger time step size, a larger portion of the network can be put on a computation unit. This translates into a larger margin of the difference between the worst and the best. In other words, even though the worst (or the largest) computational burden is orders of magnitude larger than the best (or the smallest) computational burden, all the necessary computation can still be completed within the given amount of time. However, the requirement of real-time makes the margin much smaller. In other words, the difference between the worst and the best should be as small as possible in order to ensure the even distribution of the computational load. Besides, data exchange/communication is essential in parallel computation, affecting the overall performance. However, the exchange of data takes time. Therefore, the corresponding consideration needs to be with the computational load distribution among multiple calculation units. If it turns out in a satisfactory way, such distribution will raise the possibility of completing the necessary computation in a given amount of time, which might come down in the level of microsecond order. This paper presents an effective way to split a given electrical network, according to multiple criteria, for the purpose of distributing the entire computational load into a set of even (or close to even) sized computational loads. Based on the proposed system splitting method, heavy computation burdens of large-scale electrical networks can be distributed to multiple calculation units, such as an RTDS real time simulator, achieving either more efficient usage of the calculation units, a reduction of the necessary size of the simulation time step, or both.

Histopathological features of pacific whiteleg shrimp, Litopenaeus vannamei, infected with Infectious Myonecrosis Virus (IMNV) with an emphasis on micro-traumas and inflammatory responses in muscle tissues (전염성근괴사증바이러스(IMNV)를 인위감염 시킨 흰다리새우(Litopenaeus vannamei)의 근육에서 나타난 미세 손상과 염증반응에 대한 조직병리학적 특성 연구)

  • HyoEun, Lee;YoungSook, Kim;JinHyeon, Jang;WonJoo, Chun;GaYoung, Choi;Bambang, Hanggono;SuMi, Kim
    • Journal of fish pathology
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    • v.35 no.2
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    • pp.167-176
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    • 2022
  • We injected infectious myonecrosis virus (IMNV) to pacific whiteleg shrimp, Litopenaeus vannamei, and observed closely with using light microscope and transmission electron microscope (TEM) for 4-8 days post infection (dpi). As clinical signs, abdominal bodies had mild opaque muscles at 5 dpi. And the mortality was shown at 6 dpi. At 8 dpi, most injected shrimps had severe opaque muscles and humped back that cause of movement disorder. As results of histopathological examinations, local parts of abdominal body muscle had muscle fiber hyalinization, muscle fiber atrophy, rounded muscle fibers, myofibrillar hypertrophy in size, a decrease in number of myofibrils and phagocytosis from the sarcolemmas by multiple hemocytes at 4 dpi. Especially, myofibrillar hypertrophy appeared at the whole or random part of single muscle fiber not in specific locations like the center or edge of muscle fiber. At 6-7 dpi, multiple muscle necrosis, muscle fiber segmentation, myofibril lysis ap- peared and a few hemocytes were infiltrated at lesions. At 8 dpi, extensive muscle necrosis, multiple myofibril lysis and muscle fiber atrophy were shown, and very few hemocytes were infiltrated. In early stage of infection, local viral myositis with zenker's degeneration were shown. These lesions appeared multiply after the early stage. In late stage of infection, extensive coagulative muscle necrosis appeared with few of inflammatory response such as hemocytes infiltration. The lack of hemocytes infiltration response at the late stage might be disadvantage for Litopenaeus vannamei to defense against IMNV and to recover, because hematocytes (granulocyte, semi-granulocyte) eliminate pathogen and damaged tissues from infection sites and help recover. As results of the TEM observation, IMNVs that had nonenveloped icosahedral capsid which was 30-40 nm diameter were in myofibril and beside tubules of sarcoplasmic reticulum and moved to the certain direction. The micro-tears and micro-trau- mas in myofibrils caused muscle fiber necrosis. And semi-granulocytes engulfed IMNVs to eliminate virus.

A Study on the Remodeling Plans of Rural Villages for a Pleasant Settlement Environment (쾌적한 정주환경 조성을 위한 농촌마을 리모델링 추진 방안 연구)

  • Kim, Sang Bum;Son, Ho Gi;Shin, Min Ji
    • Journal of recreation and landscape
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    • v.6 no.2
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    • pp.27-36
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    • 2012
  • This study, presents the details of a propulsion system for a systematic approach to the remodeling of rural villages as part of the pilot project that will be implemented in 2013. Studies on remodeling rural villages have focused mainly on various concepts of remodeling purposes and functions, and on the size and allocation standards of the facilities, such as rural houses, village rest areas, or public facilities. Thus, research is required on the establishment of a plan to restructure rural spaces and suggestions regarding the standards applicable at the local sites. This study employed an upward access system to prepare for the remodeling project and encourage the local residents' participation. As a result, the local residents voluntarily participated in drawing up a comprehensive plan for the village maintenance settings and forms, and for making the arrangements, by discussing the pros and cons of the plan, or through the forum. Based on this result, the local government examined the project in terms of its specifics, size, timing, budgetary appropriation, and anticipated results in order to implement the project, and also provided aid from the field experts to review the plan for the space and facility plans, and the legal system to establish the remodeling plan for the rural villages. Thus, this study attempts to contribute toward improving the quality of the rural villager's lives through a comprehensive remodeling of the whole rural village, not through an individual remodeling method.

A Study on Lee Hae-Rang's Realism and Direction Standpoint - Focusing on The Performance Direction of Text "Hamlet" - (이해랑의 리얼리즘과 연출 관점에 대한 소고 - 텍스트 "햄릿" 공연 연출을 중심으로 -)

  • Ahn, Jang whan
    • (The) Research of the performance art and culture
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    • no.22
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    • pp.327-370
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    • 2011
  • Shakespeare's text "Hamlet" was first introduced in Korea in the first part of 1920s by Hyeon Cheol via 『Gaebyeok』. Its performance of whole acts was realized in Kinema Theater in Daegu by the direction of Lee Hae-Rang (translated by Han Lo-Dan) in September, 1951, during the Korean War. Since then, a variety of performances were carried out by numberless performing artists and performing groups in the 1960s, 1970s, 1980s and 1990s. The purpose of this study was, among numberless performing artists and performances appeared in the history of performance of "Hamlet", to examine Lee Hae-rang's direction standpoint of "Hamlet", which has been one of the mainstays since the 1950s. For this, among many performances directed by Lee Hae-rang, the investigator referred to the performing scripts and performance criticisms for the opening performance of Drama Center in 1962 and the performances in HOAM Art Hall in 1985 and 1989, focusing on the text "Hamlet" performance in 1951. In the second chapter, the concept, standpoint and background of realism, the base of his theatrical activities in his lifetime, were examined. In the third chapter, before analyzing his direction standpoint for text "Hamlet", the traditional and modern concept of text was summarized and a variety of standpoints and viewpoints for the text were analyzed. And based on the above summary and analysis, his direction standpoint was analyzed and examined, thus presenting a clue for the discussion on the position of Shakespeare's text "Hamlet" directed by Lee Hae-rang in the Korean history of performance and its performance aesthetics.

Running to Change Prejudice into Hope - A Qualitative Case Study on Academically talented Children in Residential Care - (편견을 희망으로 바꾸는 달리기 - 학업성취 우수 시설보호아동에 관한 질적 사례연구 -)

  • Kim, Seohyun;Yang, Eunbyeor;Chung, Ick-Joong
    • Korean Journal of Social Welfare
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    • v.69 no.4
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    • pp.177-202
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    • 2017
  • We usually expect that children in residential care are not able to have excellent academic achievement, even though their school achievement in adolescence is crucial as a part of whole lifespan development. The purpose of this study is to carefully understand characteristics and experiences of only a few academically talented children in residential care and to find out the practical suggestion to support the academic performance of children in residential care. For this purpose, we had interviewed eight children in depth and analyzed the data using a qualitative case study method. As a result, we found a total of 21 subcategories and 5 categories. The categories included that 'always being faithful despite being not fast', 'believing myself when I face limitations', 'conflict in high support and high expectation', 'sometimes refusing to support on me, but I am leaning on my mind', 'relieving anxiety by studying'. In conclusion, we found that the central theme of 'running to change prejudice into hope' were found through the cases with excellent academic achievement. Based on the results, we suggested the guidelines to consider when developing and providing the academic support services for children in residential care.

Geochemical Implication of Rare Earth Element pattern and Rb-Sr mineral isochron from consituent minerals in the Naedeokri-Nonggeori granite, Yeongnam Massif, Korea (영남육괴 북동부 내덕리-농거리 화강암내 구성광물의 희토류원소 분포도 및 Rb-Sr 광물연대의 지구화학적 의의)

  • Seung-Gu Lee;SeungRyeol Lee
    • Korean Journal of Mineralogy and Petrology
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    • v.36 no.2
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    • pp.125-134
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    • 2023
  • The Naedeokri and Nonggeori granites are early Proterozoic granites of the Taebaek-Sangdong area in the northeastern part of the Yeongnam Massif. In this paper, rare earth elements (REEs) concentrations of the minerals in Naedeokri and Nonggeori granites and Rb-Sr mineral isochron age are reported. Except zircon, the constituent minerals such as mica, feldspar, quartz, and tourmaline show LREE-enriched and HREE-depleted REE patterns with relatively large Eu negative anomaly. However, zircon has geochemical characteristic of LREE- and HREE-enriched REE pattern with large Eu positive anomaly. This pattern suggests that zircon should be hydrothermal zircon due to deuteric hydrothermal alteration. In addition, the Rb-Sr mineral age of Naedeokri granite indicates an age value of 1.814±142(2σ) Ma. The Rb-Sr whole rock age including pervious data of Naedeokri and Nogggeori granite indicates an age value of 1,707±74(2σ) Ma. This value is younger than the Sm-Nd isochron of 1.87 Ga, indicating that the Rb-Sr isotope system may be re-homogenized by hydrothermal alteration during the transition from a magmatic to a hydrothermal system.

Resistance Factors of Driven Steel Pipe Piles for LRFD Design in Korea (LRFD 설계를 위한 국내 항타강관말뚝의 저항계수 산정)

  • Park, Jae Hyun;Huh, Jungwon;Kim, Myung Mo;Kwak, Kiseok
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.28 no.6C
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    • pp.367-377
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    • 2008
  • As part of study to develop LRFD (Load and Resistance Factor Design) codes for foundation structures in Korea, resistance factors for static bearing capacity of driven steel pipe piles were calibrated in the framework of reliability theory. The 57 data sets of static load tests and soil property tests conducted in the whole domestic area were collected and these load test piles were sorted into two cases: SPT N at pile tip less than 50, SPT N at pile tip equal to or more than 50. The static bearing capacity formula and the Meyerhof method using N values were applied to calculate the expected design bearing capacities of the piles. The resistance bias factors were evaluated for the two static design methods by comparing the representative measured bearing capacities with the expected design values. Reliability analysis was performed by two types of advanced methods: the First Order Reliability Method (FORM), and the Monte Carlo Simulation (MCS) method using resistance bias factor statistics. The target reliability indices are selected as 2.0 and 2.33 for group pile case and 2.5 for single pile case, in consideration of the reliability level of the current design practice, redundancy of pile group, acceptable risk level, construction quality control, and significance of individual structure. Resistance factors of driven steel pipe piles were recommended based on the results derived from the First Order Reliability Method and the Monte Carlo Simulation method.

An Analysis on Reasoning of 4th-Grade Elementary School Students in Comparing Unlike Fraction Magnitudes (초등학교 4학년 학생들의 이분모 분수 크기 비교에 나타나는 추론 분석)

  • Yoon, Chaerin;Chang, Hyewon
    • Education of Primary School Mathematics
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    • v.26 no.3
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    • pp.181-197
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    • 2023
  • The importance of reasoning processes based on fractional concepts and number senses, rather than a formalized procedural method using common denominators, has been noted in a number of studies in relation to compare the magnitudes of unlike fractions. In this study, a unlike fraction magnitudes comparison test was conducted on fourth-grade elementary school students who did not learn equivalent fractions and common denominators to analyze the reasoning perspectives of the correct and wrong answers for each of the eight problem types. As a result of the analysis, even students before learning equivalent fractions and reduction to common denominators were able to compare the unlike fractions through reasoning based on fractional sense. The perspective chosen by the most students for the comparison of the magnitudes of unlike fractions is the 'part-whole perspective', which shows that reasoning when comparing the magnitudes of fractions depends heavily on the concept of fractions itself. In addition, it was found that students who lack a conceptual understanding of fractions led to difficulties in having quantitative sense of fraction, making it difficult to compare and infer the magnitudes of unlike fractions. Based on the results of the study, some didactical implications were derived for reasoning guidance based on the concept of fractions and the sense of numbers without reduction to common denominators when comparing the magnitudes of unlike fraction.

Target Reliability Indices of Static Design Methods for Driven Steel Pipe Piles in Korea (국내 항타강관말뚝 설계법의 목표 신뢰도지수)

  • Kwak, Kiseok;Huh, Jungwon;Kim, Kyung Jun;Park, Jae Hyun;Lee, Juhyung
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.28 no.1C
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    • pp.19-29
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    • 2008
  • As a part of study to develop LRFD (Load and Resistance Factor Design) codes for foundation structures in Korea, reliability analyses for driven steel pipe piles are performed and the target reliability indices are selected carefully. The 58 data sets of static load tests and soil property tests conducted in the whole domestic area were collected and analyzed to determine the representative bearing capacities of the piles. The static bearing capacity formula and the Meyerhof method using N values are applied to calculate the expected design bearing capacity of the piles. The resistance bias factors were evaluated for the two static design methods by comparing the representative bearing capacities with the design values. Reliability analysis was performed by two types of advanced methods: First Order Reliability Method (FORM), and Monte Carlo Simulation (MCS) method using resistance bias factor statistics. The static bearing capacity formula exhibited relatively small variation, whereas the Meyerhof method showed relatively high inherent conservatism in the resistance bias factors. Reliability indices for safety factors in the range of 3 to 5 were evaluated respectively as 1.50~2.89 and 1.61~2.72 for both of the static bearing capacity formula and the Meyerhof method. The target reliability indices are selected as 2.0 and 2.33 for group pile case and 2.5 for single pile case, based on the reliability level of the current design practice and considering redundancy of pile group, acceptable risk level, construction quality control, and significance of individual structure.