• Korean Journal of Plant Taxonomy
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• v.40 no.3
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• pp.186-191
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• 2010

Four unrecorded species (Barbella flagellifera, Isothecium hakkodense, Mnium orientale and Fissidens gardneri) and with one unrecorded genus, Barbella, are reported new to the Korean moss flora. B. flagellifera (Meteoriaceae) was found on a tree at alt. 300-350 m of parasitic Seogeomun-oreum Volcano on Jeju Island. The species are mainly distributed in subtropic regions. I. hakkodense (Lembophyllaceae) was found around the top of Seongin-bong on Ulleung Island. This species is similar to I. subdiversiforme, however, it can be distinguished by the morphologies of branch leaves and alar cells. M. orientale (Mniaceae) was collected from Mt. Jiri. This is similar to M. heterophyllum, however, it can be distinguished by plant size, number of teeth in leaf margin and cell size on leaf. F. gardneri (Fissidentaceae) was found along the valley of Mt. Palyeong, Jeollanam Province. This species is similar to F. obscurirete, but distinguished by the size of the plants and their leaves as well as the costal lengths of the leaves.

• CELLMED
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• v.11 no.3
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• pp.14.1-14.9
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• 2021

Background: Cuscuta reflexa Roxb is a member of the Cuscutaceae family, and in Unani medicine, it is known as Aftimoon. It is a parasitic plant that can be found growing abundantly on various host plants in India up to 3000 metres in altitude during the rainy season. Unani physicians have been using it for years to cure a variety of illnesses, including psychiatric illnesses like melancholia, schizophrenia, and epilepsy. It has been used to cure hepatitis, palpitations, and skin disorders, among other things. Objective of the study: To evaluate anti-anxiety effect of Cuscuta reflexa Roxb in Swiss Albino mice of either sex. Materials and Methods: A total of 24 Swiss Albino mice weighing 25-35 g were used in this study. Animals were chosen at random and held in their cages for at least 7 days in a standard setting. Group A was given regular saline as a vehicle, Group B was given a hydro alcoholic extract of the lower dose of the test drug, Group C was given a hydro alcoholic extract of the higher dose of the test drug, and Group D was given the standard drug Diazepam 5 mg/kg orally. Aftimoon as hydro alcoholic extract (200 mg/kg and 400 mg/kg body wt.) was given in single and double doses and observed for 7 days. Results: For each parameter in each category, mean and standard deviations were computed. For multiple group comparisons, a one-way ANOVA was used, followed by Turkey's post hoc test. (p<0.05) was used as the significance standard. Conclusion: These results advocate that the Aftimoon as double dose (400 mg/kg body wt.) revealed anti-anxiety effect similar to standard drug.

• The Korean Journal of Pesticide Science
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• v.20 no.1
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• pp.56-65
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• 2016

Pine wood nematode, Bursaphlenchus xylophilus is a fatal damaging pest, infecting pinewood trees and is one of the serious forest pest in Korea. Trunk injection is one of the most effective method to protect the live pine trees from nematode infection. The aim of this research was to reveal the efficacy of the nematicidal materials from 46 Kyrgyzstan, 88 Vietnam plant materials and 21 active materials (${\alpha}$-terpinene, ${\alpha}$-pinene, ${\beta}$-thujaplicin, cinnamaldehyde, eugenol, emodin, geraniol, limonone, methyl palmitate, matrine, myrcene, methyl gallate, nicotine, quassin, resveratrol, rotenone, thymol, thujaplicin, terthiophene, tuberstemonine, ${\gamma}$-terpinene) isolated from plants for using as trunk injection agents. Eclipta prostrata extract was highly effective against pine wood nematode in plant extracts and terthiophene has the best nematicidal activity from active materials isolated from plants. Foliar spray of terthiophene on Japanese black pine (Pinus thunbergii) tree reduced proliferation of inoculated pine wood nematode at 22 days after treatment, however no efficacy was found over 22 days. Eclipta prostrata extract and terthiophene can be used as nematicide for further nematicidal efficacy test against any other plant parasitic nematodes and trunk injection efficacy against pine wood nematode.

• Journal of Life Science
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• v.25 no.7
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• pp.833-838
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• 2015

Multidrug-resistant super bacterial, fungal, viral, and parasitic infections are major health threaten pathogens. However, to overcome the present healthcare situation, among the leading alternatives to current drugs are antimicrobial peptides (AMPs), which are abundantly produced via various species in nature. AMPs, small host defense proteins, are in charge of the innate immunity for the protection of multicellular organisms such as fish, amphibian, reptile, plants and animals from infection. The number of AMPs identified per year has increased steadily since the 1980s. Over 2,000 natural AMPs from bacteria, protozoa, fungi, plants, and animals have been listed into the antimicrobial peptide database (APD). The majority of these AMPs (>86%) possess 11–50 amino acids with a net charge from 0 to +7 and hydrophobic percentages between 31–70%. This report classified AMP into several categories including biological source, biological functions, peptide properties, covalent bonding pattern, and 3D structure. AMP functions not only antimicrobial activity but facilitates cell biological activity such as chemotatic activity. In addition, fibroblastic reticular cell (FRC) originated from mouse lymph node stroma induced the expression of AMP in inflammatory condition. AMP induced from FRC contained whey acidic protein (WAP) domain. It suggests that the classification of AMP will be done by protein domain.

• Korean journal of applied entomology
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• v.3
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• pp.31-39
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• 1964

The authors studied on the bionomics of the Florida wax scale, Ceroplastes floridensis Comstock and bionomics of two kind of parasites which attack on this Florida wax scale in 1963 to 1964. 1. Ceroplastes floridensis Comstock was heavily infested on the persimmon tree planted in the southern part of Korea, specially at the center of Taegu city. Parasitic ratio of important parasite of Ceroplastes floridensis Comstock, Microterys clauseni was $66.67\%$ and appeared low parasited ratio at the center of the city. 2. The developed eggs of the Ceroplastes floridensis Comstock was appear며 from mid-May to early July. The peak of it was late-May to early June. Average stored eggs inside of a body is 2030. $2030.77\pm0.49.$. 3. The hatching period of the Ceroplastes floridensis Comstock begins from early June to early July. Mid-June was the peak period of it. Pupation period of the male was August 14 to September 7 and adult was emerged on August 20 to September 8. Mean longevity for adult male was 3 days in August but 4 days in September. 4. On the differences of the body- length parasited on the three host plants, persimmon, platanus, and trifoliate orange, the body length parasited on the persimmon was the largest, medium on platanus and the smallest on the trifoliate orange. 5. Microterys clauseni H. Compere parasite on tile Ceroplastes floridensis Comstock with 5 generations in a year. Hibernate in the body of the host as tan·at stage. The first adult appears in middle April. Fifteen days was required for one generation in $21^{\circ}$. The authors observed on the form ovary, female with 6 ovarioles, and mean number of eggs per female was $47.19\pm0.55$. 6. On the parasited individuals of the Microterys clauseni H. Compere a larva was the most numerous and was found 2 individuals or 4 individuals parasite on the one Ceroplastes floridensis Comstock. 7. Coccophagus app. parasited on the only male body of the Ceroplastes floridensis Comstock. The parasitic ratio was $14.831\%$ on persimmon tree in Taegu city.

• Journal of Life Science
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• v.28 no.3
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• pp.369-375
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• 2018

Mycoplasma genitalium has 367 conserved genes and the smallest genome among mono-culturable prokaryotes. Conservative metabolic pathways were examined among M. genitalium and 14 prokaryotes, one hyperthermophilic exosymbiotic archaeon Nanoarchaeum equitans and 13 intracellular eubacteria of plants or insects, with fewer conserved genes than M. genitalium. They have 11 to 71 metabolic pathways, however complete metabolic pathways ranged from 1 to 24. Totally, metabolic pathway hole is very high due to the lack of 45.8% of the enzymes required for the whole metabolic pathways and it could be suggested that the shared metabolic pathway with the host's enzyme would work or the essential substances are host dependent. The number of genes necessary for mass transfer through the cell membrane is also very low, and it may be considered that the simple diffusion or the protein of the host will function in the cell membrane of these prokaryotes. Although the tRNA charging pathway was distributed in all 15 prokaryotes, each has 5-20 tRNA charging genes. This study would give clues to the understanding of the metabolic pathways of intracellular parasitic bacteria of plant and endosymbiotic bacteria of insects, and could provide basic data for prevention of crop damage, development of insect pests and human medicines.

• Korean Journal of Plant Taxonomy
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• v.43 no.4
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• pp.252-262
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• 2013

During the floristic survey on Phnom Bokor National Park, Kampot, Cambodia, we encountered Balanophora fungosa var. indica, which is a tropical holoparasitic plant. To identify its host species, we collected host roots and trees nearby and tried to identify them using DNA barcoding approach. We applied plastid rbcL and matK gene regions as DNA barcode markers, and successfully amplified and sequenced the markers from 15 host roots and seven tree samples. Obtained host root sequences were identified as Primulaceae, Celastraceae, Myrtaceae, and Oleaceae, while trees nearby are Oleaceae, Myrtaceae, Sapindaceae, Rosaceae, Clusiaceae, Ericaceae, and Lauraceae. At genus level, host species are identified as Myrsine, Euonymus, Syzygium, and Olea, but failed in species discrimination. Myrsine (Primulaceae) and Olea (Oleaceae) are reported here as host species of B. fungosa var. indica for the first time. Further sampling and comparative work, and DNA barcoding will help recognize the biodiversity of the area and host species of Balanophora, together with their evolution.

• Journal of Life Science
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• v.26 no.9
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• pp.1074-1081
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• 2016

Mistletoes are hemi-parasitic plant growing on different host tree and shrubs. They are traditionally used in folkloric medicine for the treatment of diarrhea, cough, diabetes, hypertension, cancer and skin infection. The purpose of this study was to determine the contents of phenolics and antioxidant activity of 70% ethanol, 100% methanol and hot water extracts of Jeju camellia mistletoe (Korthalsella japonica Engl.). Ethanol was most effective in extracting total phenols (7,427 mg gallic acid equivalent (GAE)/100 g) and flavonoid (1,777 mg rutin equivalent (RE)/100 g). The free radical scavenging activity, 1,1-diphenyl-2-picryl hydrazyl (DPPH) (EC₅₀ = 7.8 mg/ml) and hydrogen peroxide (H₂O₂) (EC₅₀ = 1.4 mg/ml), and the capacity for chelating metal ions (EC₅₀ = 8.0 mg/ml) and reducing power (EC₅₀ = 14.9 mg/ml) of the samples also higher in ethanolic extracts. The strong correlation (r² = −0.996～−0.881) between antioxidant capacities and the phenolic contents implied that phenolic compounds are a major contributor to the antioxidant activity of the ethanolic extracts of Jeju camellia mistletoe. As conclusions, Jeju camellia mistletoe contains bioactive substances with a potential for reducing the physiological as well as oxidative stress and this could explain the suggested cancer preventive effect of these plants as well as their protective role on other major diseases.

• Korean Journal of Plant Resources
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• v.28 no.1
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• pp.26-34
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• 2015

This study was conducted to examine the morphological characteristics and variations of the 6 populations of Pourthiaea. villosa (Thunb.) Decne. var. villosa in Korea. The ANOVA results showed that the populations were significantly different in 18 of the 21 quantitative characteristics that were analyzed. In the results of principal component analysis, 6 principal components (PC) represented 68.28% for the total variations. 'Petiole length' (0.764), 'Leaf shape' (0.834), 'Leaf length' (0.753), 'Crown diameter' (0.663), 'Inflorescence width' (0.492), and 'Leaf base shape' (0.721) showed the highest contribution to PC1, PC2, PC3, PC4, PC5, and PC6, respectively. According to the results of cluster analysis, one cluster comprised only 'Goesan-gun Ssanggok Valley' population. 'Namhae-gun Mijori Evergreen Forest' population was grouped with 'Hamyang-gun Sang Forest' population, which was the nearest subgroup. Further, 'Namhae-gun Geum Mountain' population, 'Jindo-gun Cheomchal Mountain' population, and 'Jeju-si Barimae Parasitic volcano' population were placed in the same cluster.

• Korean Journal of Environmental Biology
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• v.40 no.4
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• pp.423-432
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• 2022

The mass-rearing system for Neodryinus typhlocybae as a biological control agent of Metcalfa pruinosa was established. Depending on the density of host nymphs and plants, the average number of cocoons produced by the parasitoids was 5-8 and 70-150 cocoons per leaf and sapling of mulberry, respectively. There is a significant difference in cocoon length between females (6.10-6.46mm) and males (4.20-4.62mm). Sex determination of cocoons before emergence will be helpful for efficiently releasing this parasitoid in fields. The parasitic rate of N. typhlocybae at the semi-field condition was on average 13-17%. The release number of this parasitoid did not affect parasitism. Nevertheless, the population growth rate of M. pruinosa was reduced by increasing the release number of N. typhlocybae. The parasitoid offspring's sex and bivoltine were influenced by the host age. On young host nymphs, the bivoltine portion of parasitoid increased. When parasitized on 4^th or 5^th nymphs, the offspring's female ratio of N. typhlocybae increased. This result may be useful for potentially controlling mass rearing production of parasitoid.