• BMB Reports
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• 제52권8호
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• pp.475-481
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• 2019

The evolution of genome editing technology based on CRISPR (clustered regularly interspaced short palindromic repeats) system has led to a paradigm shift in biological research. CRISPR/Cas9-guide RNA complexes enable rapid and efficient genome editing in mammalian cells. This system induces double-stranded DNA breaks (DSBs) at target sites and most DNA breakages induce mutations as small insertions or deletions (indels) by non-homologous end joining (NHEJ) repair pathway. However, for more precise correction as knock-in or replacement of DNA base pairs, using the homology-directed repair (HDR) pathway is essential. Until now, many trials have greatly enhanced knock-in or substitution efficiency by increasing HDR efficiency, or newly developed methods such as Base Editors (BEs). However, accuracy remains unsatisfactory. In this review, we summarize studies to overcome the limitations of HDR using the CRISPR system and discuss future direction.

• 기록학연구
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• 제6호
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• pp.41-72
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• 2002

Electronic environment affects archival community to a great extent. It redefines virtually every stage of archival management form creation to current and semi-current use, to appraisal, and to preservation of records. Faced with the problems caused by ever increasing electronic records, the community is forced to reconsider traditional concepts, approaches, methodologies, even the basic paradigm embedded in archival theory and practice. The present paper discusses the need to reexamine principles and techniques of archival management in the light of digital environment. It also urges archives and archival institutions, the archival profession, or the archival community at large, to participate in this critical enterprise. Success in this endeavor will, eventually, pave the road toward creating, organizing, providing access to, preserving reliably and authentically electronic records and designing proper system for the societal collective memory in recorded digital information.

• 디지털산업정보학회논문지
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• 제14권4호
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• pp.27-33
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• 2018

The recent efforts in academia and industry represent a paradigm shift that will extend the IoT from the home environment so that it is interoperable with the Internet of Vehicles (IoV). IoV is a special kind of IoT. It allows to connect between vehicle and things located in infrastructure. Furthermore, IoV enable to create new intelligent services through collaboration with existing various services such as smart city and connected home. In this paper, we develop a service in order to realize IoV. To this end, we design a novel vehicle service platform which could automatical controlling the IoT device according to drivers' voice. To show practical usability of our proposed platform, we develop a prototype service could be call car-to-thing (C2T). We expect that our proposed platform could eventually contribute to realizing IoV.

• Clinical and Experimental Pediatrics
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• 제62권7호
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• pp.245-251
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• 2019

Hemodynamically significant preterm patent ductus arteriosus (PDA) affects mortality; comorbidities such as necrotizing enterocolitis, intraventricular hemorrhage, and bronchopulmonary dysplasia; and adverse long-term neurodevelopmental outcomes in preterm infants, particularly in very low birth weight infants. However, recent studies have indicated that there is no consensus on the causal relationship between PDA and neonatal outcomes, the benefit of PDA treatment, the factors guiding the need for treatment, and optimal treatment strategies. Such uncertainty has resulted in wide variations in practice for treating preterm PDA between units, regions, and nations. Nowadays, there has been a paradigm shift to more conservative treatment for preterm PDA, and suggestions regarding selective management of preterm PDA considering risk factors and hemodynamic significance are increasing. Neonatologist-performed echocardiography and advances in modalities to assess hemodynamic significance such as biologic markers and near-infrared spectroscopy also help improve the efficacy of selective treatment of preterm PDA.

• 전자통신동향분석
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• 제34권5호
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• pp.91-98
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• 2019

Optical frequency comb generators have been investigated as a signal source capable of generating highly stabilized ultrafast pulse lasers. The precise control of the optical frequency comb spacing by RF clock signals has led to a revolutionary paradigm shift in the precise measurement of time and frequency. Optical frequency combs also have advantages such as stable frequency spacing, stable number of lines, and robustness. Owing to these characteristics, optical frequency combs have been applied to the fields of high precision optical clock, communication, spectroscopy, waveform generation, and astronomy. In this article, we introduce the properties (i.e., generation methods, advantages, and so on) of various optical frequency combs, and discuss the expected future technological trends and applications.

• Journal of Yeungnam Medical Science
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• 제38권3호
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• pp.183-193
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• 2021

Since its discovery in 1780, lactate has long been misunderstood as a waste by-product of anaerobic glycolysis with multiple deleterious effects. Owing to the lactate shuttle concept introduced in the early 1980s, a paradigm shift began to occur. Increasing evidence indicates that lactate is a coordinator of whole-body metabolism. Lactate is not only a readily accessible fuel that is shuttled throughout the body but also a metabolic buffer that bridges glycolysis and oxidative phosphorylation between cells and intracellular compartments. Lactate also acts as a multifunctional signaling molecule through receptors expressed in various cells and tissues, resulting in diverse biological consequences including decreased lipolysis, immune regulation, anti-inflammation, wound healing, and enhanced exercise performance in association with the gut microbiome. Furthermore, lactate contributes to epigenetic gene regulation by lactylating lysine residues of histones, accounting for its key role in immune modulation and maintenance of homeostasis.

• Journal of Preventive Medicine and Public Health
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• 제54권1호
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• pp.81-84
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• 2021

The delivery of high-quality antenatal care is a perennial global concern for improving maternal and neonatal outcomes. Antenatal care is currently provided mainly on a one-to-one basis, but growing evidence has emerged to support the effectiveness of group antenatal care. Providing care in a small group gives expectant mothers the opportunity to have discussions with their peers about certain issues and concerns that are unique to them and to form a support system that will improve the quality and utilization of antenatal care services. The aim of this article is to promote group antenatal care as a means to increase utilization of healthcare.

• 한국정보처리학회:학술대회논문집
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• 한국정보처리학회 2013년도 춘계학술발표대회
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• pp.175-178
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• 2013

Mobile Virtual Desktop Infrastructures (mVDI) are gaining popularity in cloud computing by allowing mobile devices to execute their mobile applications in a cloud server instead of relying on physical mobile devices. Consolidating many users into mVDI environment can significantly lower IT management expenses and enables new features such as "available-anywhere" desktops. However, there are many barriers to broad adoption including the slow performance of virtualized I/O, CPU scheduling interference problems. In this paper, we will discuss about mVDI with the current issues, the corresponding solutions and challenges.

• 식품과학과 산업
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• 제51권2호
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• pp.114-126
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• 2018

The production of high quality oil to meet new standard needs a 'next generation' innovative oil refining tool in paradigm shift. 'Nanoneutralization' using controlled hydrodynamic cavitation-assisted Nanoreactor is successfully being introduced and commercialized into edible oil industry and it plays a key driver for sustainable development of food processing. This emerging technology using bubble dynamics as a consequence of Bernoulli's principle by hydrodynamic cavitation in Venturi-designed multi-flow through cell is radically changing the conventionally chemical-oriented neutralization. Nanoneutralization derived by the creation of nanometer-sized bubbles formed through scientifically structured geometric channels under high pressure has been proven to improve mass transfer and reaction rate so substantially reduce the chemicals required for refined vegetable oil and to increase oil yield while even improving oil quality. More researches on science behind this revolutionary technology will help usto better understand the principle and process hence makes its potential applications expandable in extraction, refining and modification of fats and oils processing.

• 방사성폐기물학회지
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• 제19권1호
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• pp.61-74
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• 2021

A paradigm shift in the government's energy policy was reflected in its declaration of early closure of old nuclear plants as well as cancellation of plans for the construction of new plants. To this end, unit 1 of Kori Nuclear Power Plant was permanently shut down and is set for decommission. Based on these changes, the off-site transport of spent fuels from nuclear power plants has become a critical issue. The purpose of this study is to develop an optimized method for transportation of spent fuels from Kori Nuclear Power Plant's units 1, 2, 3, and 4 to an assumed interim storage facility by simulating the scenarios using the Flexsim software, which is widely used in logistics and manufacturing applications. The results of the simulation suggest that the optimized transport methods may contribute to the development of delivery schedule of spent fuels in the near future. Furthermore, these methods can be applied to decommissioning plan of nuclear power plants.