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Oxygen Toxicity of Superoxide Dismutase-Deficient Saccharomyces cerevisiae by Paraquat (Paraquat에 의해 유도된 Superoxide Dismutase 결핍 효모의 산소 독성)

  • 김지면;남두현용철순허근
    • KSBB Journal
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    • v.10 no.5
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    • pp.561-567
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    • 1995
  • Using superoxide dismutase (SOD)-deficient mutants of Saccharomyces cerevisiae, the oxygen toxicity induced by paraquat was studied. In aerobic culture condition, yeasts lacking MnSOD (milochondrial SOD) showed more significant growth retardation than CuZnSOD (cytoplasmic SOD)-deficient yeasts. However, not so big differences in growth pattern of those mutants compared with wild type were observed under anaerobic condition. When exposed to paraquat, the growth of yeasts lacking CuZnSOD was severely affected by higher than 0.01mM of paraquat in culture medium. By the analysis of several cellular components ivolved in free radical generating and scavenging system, it was found that, under aerobic condition, the content of lipid peroxides in cell membrane as well as cellular activity of glutathion peroxidase of CuZnSOD-deficient mutants was increased in the presence of paraquat, although significant decrease of catalase activity was observed in those stratns. In MnSOD-deficient yeast, however, increment in cellular activity of glutathion peroxldase and catalase by paraquat was observed without any deterioration of membrane lipid. It implies that the lack of mitochondrial SOD could be compensated by both of glutathion peroxldase and catalase, but that only glutathion peroxidase might act for CuZnSOD in cytoplasm. In contrast, all of SOD-deficient mutants showed a significant decrease in catalase activity, but slight increase in the activities of glutathion peroxidase, when cultivated anaerobically in the medium containing paraquat. Nevertheless, any significant changes of lipid peroxides in cell membranes were not observed during anaerobic cultivation of SOD-deficient mutants. It suggests that a little amount of free radicals generated by paraquat under anaerobic condition could be sufficiently overcome by glutathion peroxidase but not by catalase.

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Basic Analysis for Social Spreading of Family Archives (가족아카이브의 사회적 확대를 위한 시론적 분석)

  • Kim, Myoung-hun
    • The Korean Journal of Archival Studies
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    • no.66
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    • pp.229-265
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    • 2020
  • This study examined the necessity of establishing a family archive as a means for the recovery of the family community and the direction for establishing the family archive as a social culture. With the lack of research on family archives worldwide, this study analyzed family archives focusing on three areas. The first is a review on the necessity of family archives. To this end first, studies in the fields of sociology and family studies were analyzed to understand the situation in which the crisis of the family community intensified in the recent social environment, and based on this analysis, the necessity of establishing a family archive using records in Korean society was suggested. The second is case studies of National Archives of advanced countries for social expansion of family archives. In the case of advanced countries in Western, family archives are closely related to family history or genealogy research, and more than half of visitors to archives are occupied by family history or genealogy researchers. This is because National Archives of Western countries provide a variety of services for building family archives. The third is an analysis of the meaning and characteristics of family archives. This is because in order for the family archive to become a pan-social recording culture, it is necessary to establish the values and roles of the family archive along with its own meaning and characteristics different from the existing public records. Accordingly, the aim was to establish the concept and goal of the family archive, and to explore the functions and values of the family archive in comparison with the existing theories of archives. As a result of the analysis focusing on these three areas, the family archive needs to focus on 'culture', not 'institution', and 'utilization' rather than 'management'. Theories and methodologies of archival science have been developed with an emphasis on systematically managing and preserving a vast amount of records like public records, and based on highly specialized knowledge, records management has been established as an institution. However, in order to spread the family archive socially, it must be established as a culture or lifestyle that can be practiced by all ordinary citizens in the process of daily life. Prior to the management and preservation of professional records, all members of the family must understand the meaning contained in the records. It is necessary to prioritize use so that they can be shared.

Fundamental Study on the Evapo- transaration Requirements of Patty rice Plant (벼 용수량계획상의 엽면증발량 및 주간수면 증발량에 관한 기초적인 연구)

  • 김철기
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.11 no.2
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    • pp.1651-1660
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    • 1969
  • The purpose of this study is to find out the reasonable amount of evapo-transpiration required for the paddy rice plant during the whole growing season. So. On the basis of the experimental data concerning the evapo-transpiration form 1966 to 1968, the author obtained the follow results. 1) The leaf area index in the densely planted plo is generally higher than that in the conventionally planted one during the first half of growing season So, the coefficient of transpiration in the former plot is somewaht higher than in the latter, and the coefficient of water surface evaporation under the plant cover has the inverse relation between both plots. 2) It is unreasonable that coefficient of evapo-transpiration is applied to the calculation of the evapo-transpiration requirements of each growing stage, because a degree of variation in meteorological factors and in the thickness of the plant growth is involved in it. 3) It is most reasonable that the rate of transpiration and of the water surface evapoation is applied to the calculation of the transpirated amount and evaporated one in each growing stage, because it shows almost constant value in spite of any meteorological conditions in so far as the variety of rice, planted density and control of applying fertilizer are sanme and the disease and blight are negligible. 4) The ratio of the amount of transpiration to the weight of the whole air dried yields has the tendency of decreasing as that of the yields increases having almost constant value despite the amount of pan evaporation; and the value is about 210 when the weight of root parts is included to that the yields. 5) Although the required amount of transpiration during the whole growing season can be calculated with the above ratio, Fig. 7 showing the relation between the amount of transpiration and the weight of the yields is more reasonable and will be convinient to find it. And the requirements of water surface evaporation during the same season can also be directly found witht theweight air dried straw refering to Fig. 8.

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Solubility Improvement of Cuttle Bone Powder Using Organic Acids (유기산처리에 의한 갑오징어갑 분말의 가용성 개선)

  • KIM Jin-Soo;CHO Moon-LAE;HEU Min-Soo;CHO Tae-Jong;AN Hwa-Jin;CHA Yong-Jun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.36 no.1
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    • pp.11-17
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    • 2003
  • As a pan of a study on effective use of seafood processing by-products, such as cuttle bone as a calcium source, we examined on the kind of organic acid (acetic acid and lactic acid), reaction concentration (mole ratio of calcium to mole of organic acid), reaction temperature $(20\~60^{\circ}C)$ and reaction time (6$\~$24 hours) as reaction conditions for the solubility improvement of cuttle bone powder. The high soluble cuttle bone powder was also prepared from the optimal reaction conditions and partially characterized. From the results on examination of reaction conditions, the high soluble cuttle bone powder was prepared with 0.4 in mole ratio of a calcium to mole of a acetic acid at room temperature for 12 hours, Judging from the patterns of IR and X-ray diffraction, the main component of the high soluble cuttle bone powder was presented as a form of calcium acetate, and a scanning electron micrograph showed an irregular form. The soluble calcium content in the high soluble cuttle bone powder was $5.3\%$ and it was improved about 1,380 times compared to a raw cuttle bone powder. For the effective use of the high soluble cuttle bone powder as a material for a functional improvement in processing, it should be used after the calcium treatment at room temperature for about 1 hour in tap water or distilled water. from these results, we concluded that it is possible to use the high soluble cut시e bone powder as a material for a functional improvement in processing.

Contributions of Ionic Strength, pH, and Replacing Cations to the Cation Exchange Capacities of Soils (치환양(置換陽) ion의 종류(種類) 및 pH 가 토양(土壤)의 양(陽) ion 치환용량(置換容量)에 미치는 영향(影響))

  • Lim, Hyung-Sik;Kwag, Pan-Ju;Kim, Hee-Joong
    • Korean Journal of Soil Science and Fertilizer
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    • v.17 no.2
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    • pp.114-124
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    • 1984
  • Various methods for measuring cation exchange capacity (CEC) of soil were compared and the contributions of ionic strength, pH and replacing cations to the CEC were investigated on Kangweon soils (Pyeongchang soils derived from lime stone : Chuncheon, Weonseong soils from alluvium : Cheolweon soils from basalt). The results were as follows : 1. The CEC measuring method using shaker and centrifuge at saturating, washing and replacing precesses, which are common in determining CEC of soils, appeared to be superior to the other methods using column, filter, or Brown method. 2. For all soil samples, the higher the ionic strength, the higher CEC value was obtained with the fewer saturating processes. However, using monovalent saturating ion on Anmi series soil derived from lime stone, the CEC value decreased when the ionic strength and the number of saturating process increased. 3. The CEC value generally increased with increasing pH. But, Chuncheon soil (Gyuam series from alluvium) having higher Al content showed the abrupt increases of CEC from pH 5.5 to pH 7.5. 4. About 70% of CEC of Kangweon soils were attributed to organic matter. 5. In determining CEC of soils, saturating with 0.5M divalent cation solution 2 to 3 times for Pyeongchang and Weonseong soil, 3 to 4 times for Cheolweon soil, and replacing with 0.25M divalent cation solution about 3 times are thought to be recommendable.

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Manufacture of Spent Layer Chicken Meat Products by Natural Freeze-Drying during Winter (겨울철 자연 동결 건조에 의한 노계 육제품의 제조)

  • Lee, Sung-Ki;Kang, Sun-Moon;Lee, Ik-Sun;Seo, Dong-Kwan;Kwon, Il-Kyung;Pan, Jo-No;Kim, Hee-Ju;Ga, Cheon-Heung;Pak, Jae-In
    • Food Science of Animal Resources
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    • v.30 no.2
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    • pp.277-285
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    • 2010
  • The objective of this study was to manufacture spent layer chicken meat products by natural freeze-drying. The spent layers of chickens that were slaughtered at 80 wk were obtained from a local slaughter house and separated into two halves of carcasses. The samples were divided into the following groups: 1) control (non-curing), 2) curing, and 3) curing with 2% trehalose before drying. The cured meats were placed at $2^{\circ}C$ for 7 d and then transferred to a natural drying spot located in Injae City, Gangwondo, Korea. The experiment was conducted from January to March in 2008. The average temperature, RH, and wind speed were $-1.5^{\circ}C$, 63%, and 1.8 m/sec, respectively. The cured treatments showed higher pH, lower Aw and lower shear force value compared with the control. Based on the results of TBARS (2-thiobarbituric acid reactive substances) level and volatile basic nitrogen value, lipid oxidation and protein deterioration were inhibited in curing treatments during drying. Trehalose acted as a humectant because it maintained a lower water activity despite the relatively higher moisture content during drying. The polyunsaturated fatty acids content and sensory attributes were higher in cured treatments than in the control during drying. Most of the bacterial counts in the treated groups were lower by 2 Log CFU/g after 1 mon of drying, and Salmonella spp. and Listeria spp. were not found in any treatment. There was also no microbial safety problem associated with dried meat products. Based on the results of this experiment, dried meat products could be manufactured from precured spent layer chickens by natural freeze-drying during winter.

Analysis and Evaluation of Glycemic Indices and Glycemic Loads of Frequently Consumed Carbohydrate-Rich Snacks according to Variety and Cooking Method (탄수화물 간식류 식품 및 조리방법에 따른 혈당지수 및 혈당부하지수)

  • Kim, Do Yeon;Lee, Hansongyi;Choi, Eun Young;Lim, Hyunjung
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.1
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    • pp.14-23
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    • 2015
  • This study examined the glycemic indices (GIs) and glycemic loads of carbohydrate-rich snacks in Korea according to variety and cooking method. The most popular carbohydrate snacks (corn, potatoes, sweet potatoes, chestnuts, and red beans) from the Korean National Health and Nutrition Examination Survey nutrient database were cooked using a variety of conventional cooking methods (steaming, baking, porridge, puffing, and frying). The GIs of foods were measured in 60 healthy males after receiving permission from the University Hospital institutional review board (KMC IRB 1306-01). Blood glucose and insulin levels were then measured at 0, 15, 30, 60, 90, and 120 min after consuming glucose, and each test food contained 50 g of carbohydrates (corn: 170.0 g, potatoes: 359.7 g, sweet potatoes: 160.3 g, chestnuts: 134.8 g, red beans: 73.1 g). GI values for test foods were calculated based on the increase in the area under the blood glucose response curve for each subject. Steamed potatoes ($93.6{\pm}11.6$), corn porridge ($91.8{\pm}19.5$), baked sweet potatoes ($90.9{\pm}9.6$), baked potatoes ($78.2{\pm}14.5$), steamed corn ($73.4{\pm}9.9$), and steamed sweet potatoes ($70.8{\pm}6.1$) were shown to be considered high GI foods, whereas baked chestnuts ($54.3{\pm}6.3$), red bean porridge ($33.1{\pm}5.5$), steamed red beans ($22.1{\pm}3.2$), fried potatoes ($41.5{\pm}7.8$), and ground and pan-fried potatoes ($28.0{\pm}5.1$) were considered as low GI foods. The results suggest that the cooking method of carbohydrate-rich snacks is an important determinant of GI values.

Physico-chemical and Sensory Characteristics of Cooked Sausage Substituted with KCl or MgCl2 for NaCl (KCl 또는 MgCl2의 NaCl 대체 소시지의 이화학적 및 관능적 특성)

  • Jin, Sang-Keun;Kim, Il-Suk;Hur, In-Chul;Nam, Sang-Hae;Kang, Suk-Nam;Shin, Daekeun
    • Journal of agriculture & life science
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    • v.45 no.5
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    • pp.81-89
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    • 2011
  • This study was carried out to investigate changes in physicochemical and sensory properties of cooked sausages replaced sodium chloride (NaCl) to potassium chloride (KCl) or magnesium chloride ($MgCl_2$) during storage for 30 days under $4^{\circ}C$. All sausages were prepared with different combination of salts as follow; CTL (1.5% NaCl), KCL (0.9% NaCl+0.6% KCl), MCL (0.9% NaCl+0.6% $MgCl_2$), KML (0.9% NaCl+0.3% KCl+0.3% $MgCl_2$) and PST (1.5% PanSalt). Among sausages moisture content in KML was the highest (p<0.05). Lightness and redness in CTL were lower than those of other treatments, but MCL and KML containing $MgCl_2$ showed higher CIE $L^*$ and $a^*$ values than CTL. The pH in CTL was the highest during storage, however, no significant difference was determined between two treatments, MCL and KML (p>0.05). Crude fat content and water holding capacity (WHC), hardness and cohesiveness of MCL sausages were higher than those of CTL. In sensory characteristics of cooked sausages, saltness in MCL was the lowest during 10 and 20 days of storage (p<0.05). Yellowness in PST was lower than other treatmeants. Gumminess and chewiness of texture property of sausages from MCL and KML were higher than CTL. The results indicate that the replacement of NaCl by KCl improved texture, but meat color was not improved as expected. In contrast, the replacement of NaCl by $MgCl_2$ enhanced color, texture and WHC, whereas partial replacement of NaCl by $MgCl_2$ must reduce bitter taste as compared to sausages manufactured with a NaCl only. Therefore, $MgCl_2$ may be a salt replacing NaCl in cooked pork sausages.

A study on the fire characteristics according to the installation type of large smoke exhaust port in a small cross sectional tunnel fire (소단면 대심도 터널 화재시 대배기구의 설치형태에 따른 화재특성 연구)

  • Choi, Pan-Gyu;Baek, Doo-San;Yoo, Ji-Oh;Kim, Chang-Yong
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.21 no.1
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    • pp.201-210
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    • 2019
  • Recently, due to the efforts to mitigate traffic congestion and expansion of space efficiency, the construction of underground roads has been increased in big-scale cities. Since tunnels in the city have a higher chance for a fire leading to a great tragedy during a severe traffic jam than mountain tunnels, it is highly likely that it will be constructed as a tunnel, having a small cross section, for small vehicles. However, if they are constructed as such small-vehicle tunnels, it would be possible to reduce the design fire intensity while the concentration of harmful gases would increase due to a reduction in the small cross sectional area, led by a decrease in the tunnel height. In this study, behaviors of fire smoke by the installation interval and format of large-scale exhaust-gas ports were examined and compared in the analysis of temperatures and CO concentrations of a tunnel and its results were as the following. Although there were no significant differences in the smoke spreading distance between installation intervals, but in this study, 100 m was found to be the most effective installation interval. The smoke exhaustion performance was found to be excellent in the order of $4m{\times}3m$, $6m{\times}2m$, and $3m{\times}2m$ (2 lane) of the smoke spreading distance. Although there was no significant difference in the smoke spreading distance between formats of large-scale exhaust-gas ports, it was found that the smoke spreading distance was larger than other cases when it was $3m{\times}2m$ in the fire growing process. The analysis of smoke spreading distances by the aspect ratio showed that a smoke spreading distance was shorted when its the smoke spreading distance was found to be shorter when its traverse distance was relatively longer than its longitudinal distance.

Southeast Asian Hindu Art from the 6th to the 7th Centuries (6-7세기의 동남아 힌두 미술 - 인도 힌두미술의 전파와 초기의 변용 -)

  • Kang, Heejung
    • The Southeast Asian review
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    • v.20 no.3
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    • pp.263-297
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    • 2010
  • The relics of the Southeast Asian civilizations in the first phase are found with the relics from India, China, and even further West of Persia and Rome. These relics are the historic marks of the ancient interactions of various continents, mainly through the maritime trade. The traces of the indic culture, which appears in the historic age, are represented in the textual records and arts, regarded as the essence of the India itself. The ancient Hindu arts found in various locations of Southeast Asia were thought to be transplanted directly from India. However, Neither did the Gupta Hindu Art of India form the mainstream of the Gupta Art, nor did it play an influential role in the adjacent areas. The Indian culture was transmitted to Southeast Asia rather intermittently than consistently. If we thoroughly compare the early Hindu art of India and that of Southeast Asia, we can find that the latter was influenced by the former, but still sustained Southeast Asian originality. The reason that the earliest Southeast Asian Hindu art is discovered mostly in continental Southeast Asia is resulted from the fact that the earliest networks between India and the region were constructed in this region. Among the images of Hindu gods produced before the 7th century are Shiva, Vishnu, Harihara, and Skanda(the son of Shiva), and Ganesha(the god of wealth). The earliest example of Vishnu was sculpted according to the Kushan style. After that, most of the sculptures came to have robust figures and graceful proportions. There are a small number of images of Ganesha and Skanda. These images strictly follow the iconography of the Indian sculpture. This shows that Southeast Asians chose their own Hindu gods from the Hindu pantheon selectively and devoted their faiths to them. Their basic iconography obediently followed the Indian model, but they tried to transform parts of the images within the Southeast Asian contexts. However, it is very difficult to understand the process of the development of the Hindu faith and its contents in the ancient Southeast Asia. It is because there are very few undamaged Hindu temples left in Southeast Asia. It is also difficult to make sure that the Hindu religion of India, which was based on the complex rituals and the caste system, was transplanted to Southeast Asia, because there were no such strong basis of social structure and religion in the region. "Indianization" is an organized expansion of the Indian culture based on the sense of belonging to an Indian context. This can be defined through the process of transmission and progress of the Hindu or Buddhist religions, legends about purana, and the influx of various epic expression and its development. Such conditions are represented through the Sanskrit language and the art. It is the element of the Indian culture to fabricate an image of god as a devotional object. However, if we look into details of the iconography, style, and religious culture, these can be understood as a "selective reception of foreign religious culture." There were no sophisticated social structure yet to support the Indian culture to continue in Southeast Asia around the 7th century. Whether this phenomena was an "Indianization" or the "influx of elements of Indian culture," it was closely related to the matter of 'localization.' The regional character of each local region in Southeast Asia is partially shown after the 8th century. However it is not clear whether this culture was settled in each region as its dominant culture. The localization of the Indian culture in Southeast Asia which acted as a network connecting ports or cities was a part of the process of localization of Indian culture in pan-Southeast Asian region, and the process of the building of the basis for establishing an identity for each Southeast Asian region.