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Reproductive Ecology of the Hard Shelled Mussel, Mytilus coruscus in Western Korea (한국 서해산 홍합, Mytilus coruscus의 번식생태)

  • Lee, Il-Ho;Chung, Ee-Yung;Son, Pal-Won;Shin, Moon-Seup
    • The Korean Journal of Malacology
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    • v.23 no.2
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    • pp.199-208
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    • 2007
  • The gonad index, gonadosomatic index, the condition index, reproductive cycle, first sexual maturity, and sex ratio of the hard shelled mussel, Mytilus coruscus were investigated by using histological analysis and morphometric data. Specimens were collected monthly in Kyeokpo, western Korea from January to December, 2006. The gonad index reached a maximum in February, and minimum in September. Both the monthly variations of the gonad index (GI) and the gonadosomatic index (GSI) reached a maximum in February. After that, their values continually decreased between March and April because of spawning. The monthly variation of the condition index coincides with the GI and the GSI. The spawning period was from February to April, with the main spawning occurring between February and March. The reproductive cycle of this species could be classified into six stages: early active stage (November to January), late active stage (November to February), ripe stage (December to April), partially spawned stage (February to April), degenerative stage (April to October), and resting stage (June to December ). Percentages of sexual maturity was over 50% for the female and male hard shelled mussels that ranges from 40.1 to 50.0 mm in shell length. The percentage was 100% for those that are over 50.1 mm. The sex ratios of females to males over 40.1 mm in shell length were not significantly different from a 1:1 sex ratio $(x^2\;=\;0.63,\;p\;>\;0.05)$. No evidence of hermaphroditism was found in histological sections of any hard shelled mussel examined.

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Gelatinized Carbohydrates in the Diet of Catla catla Fingerlings: Effect of Levels and Sources on Nutrient Utilization, Body Composition and Tissue Enzyme Activities

  • Yengkokpam, Sona;Sahu, N.P.;Pal, A.K.;Mukherjee, S.C.;Debnath, Dipesh
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.1
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    • pp.89-99
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    • 2007
  • A Feeding trial was conducted to study the effects of three different sources and two levels of dietary gelatinized carbohydrate (GC) on nutrient utilization, growth, tissue composition and tissue enzyme activities of fingerlings of Catla catla (15.1-15.3 g). Six isocaloric (17.1-17.5 kJ/g) semi-purified diets were prepared either with rice, corn or tapioca at 40 or 50% GC each. The crude protein (CP) level used in the diet was 35% and 25% for 40% and 50% GC level, respectively to study the protein sparing effect of GC. The degree of gelatinization was higher for corn and tapioca than rice under similar cooking conditions. After a 60-d feeding trial, dry matter, carbohydrate, protein and lipid digestibility were higher in tapioca fed groups at both the levels of GC. However, the highest specific growth rate (SGR) and protein efficiency ratio (PER) were observed in the corn fed groups at 50% GC level indicating better utilization of nutrients from gelatinized corn. Feed conversion ratio (FCR) was almost similar in corn and tapioca fed groups between two levels of GC but in rice fed groups, FCR was lower in 40% GC than 50% GC level. The results indicated higher protein-sparing effect in corn and tapioca fed groups than rice fed groups. The order of gelatinized carbohydrate utilization in Catla catla fingerlings at 50% GC level was corn>tapioca>rice. At 40% GC level, corn and tapioca were comparable and more efficiently utilized than rice. In the corn fed groups, 50% GC was comparable with 40% GC level, whereas in rice and tapioca fed groups the 40% GC was better in terms of nutrient utilization. Liver glycogen content and hepatosomatic index were significantly (p<0.05) higher in those groups fed high GC (50%) irrespective of carbohydrate sources. Higher intestinal amylase and glucose-6-phosphate dehydrogenase activities were observed in higher GC fed groups than the lower GC groups. No mortality was found in any groups at any levels of GC.

A Study on Artificiality Salivary pH and Sugar Fermentation Test of Caries Potentiality Foods (우식유발식품에 의한 인공타액내 pH 변화와 당 분해 효소 활성에 관한 연구)

  • Ji, Yun-Jeong;Park, Su-Kyung
    • Journal of dental hygiene science
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    • v.12 no.4
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    • pp.303-309
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    • 2012
  • This study was designed to provide basic data for developing a standardized caries potentiality index to help to choose snack foods with a low score on the index by investigating adolescents' snack intakes and measuring them in total saccharinity, pH, and acidogenic potential and glucosidase activity of Streptococcus mutans (S. mutans). Total 28 snack foods were selected and measured for total saccharinity (Pocket refractometer PAL-1, ATAGO) and pH with a pH meter (Mentor, Seoul, Korea). Artificial saliva was added to each sample. The experiment group was administered with S. mutans (ATCC 3692), cultured in a constant temperature incubator at $37^{\circ}C$, and measured for pH changes over five times including after 10 minutes, 30 minutes, 1 hour, and 24 hours. Each sample of 0.3 ml was added to tubes containing sucrose, galactose, or glucose to measure the glucosidase activity of S. mutans. They were then observed for glucosidase activity with colorimetry after 24 hours of culture in a constant temperature incubator at $37^{\circ}C$. The mean pH by the acidogenic potential of S. mutans was pH 5.33. The experiment group dropped in pH more than the control group due to the increasing acidogenic potential of S. mutans by glucosidase activity, recording pH 5.27 after 10 minutes, pH 5.21 after 30 minutes, pH 5.15 after 1 hour, and pH 4.80 after 24 hours. The observation results of glucosidase activity of S. mutans with colorimetry show that most of the samples were positive in orange and yellow with glucose, sucrose, and glactose recording activity of 78.58%, 75%, and 71.42%, respectively.

Irradiation enduced In-plane magnetization in Fe/MgO/Fe/Co multilayers

  • Singh, Jitendra Pal;Lim, Weon Cheol;Song, Jonghan;Kim, Jaeyeoul;Asokan, K.;Chae, Keun Hwa
    • Proceedings of the Korean Vacuum Society Conference
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    • 2015.08a
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    • pp.188.1-188.1
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    • 2015
  • For present investigation Fe/MgO/Fe/Co multilayer stack is grown on Si substrate using e-beam evaporation in ultrahigh vacuum. This stack is irradiated perpendicularly by 120 MeV $Ag^{8+}$ at different fluences ranging from $1{\times}10^{11}$ to $1{\times}10^{13}ions/cm^2$ in high vacuum using 15UD Pelletron Accelerator at Inter University Accelerator Centre, New Delhi. Magnetic measurements carried out on pre and post irradiated stacks show significant changes in the shape of perpendicular hysteresis which is relevant with previous observation of re-orientation of magnetic moment along the direction of ion trajectory. However increase in plane squareness may be due to the modification of interface structure of stacks. X-ray reflectivity measurements show onset of interface roughness and interface mixing. X-ray diffraction measurements carried out using synchrotron radiation shows amorphous nature of MgO and Co layer in the stack. Peak corresponding body centered Fe [JCPDS-06-0696] is observed in X-ray diffraction pattern of pre and post irradiated stacks. Peak broadening shows granular nature of Fe layer. Estimated crystallite size is $22{\pm}1nm$ for pre-irradiated stack. Crystallite size first increases with irradiation then decreases. Structural quality of these stacks was further studied using transmission electron microscopic measurements. Thickness from these measurements are 54, 36, 23, 58 and 3 nm respectively for MgO, Fe, MgO, Fe+Co and Au layers in the stack. These measurements envisage poor crystallinity of different layers. Interfaces are not clear which indicate mixing at interface. With increase fluence mixing and diffusion was increased in the stack. X-ray absorption spectroscopic measurements carried out on these stacks show changes of Fe valence state after irradiation along with change of O(2p)-metal (3d) hybridized state. Valence state change predicts oxide formation at interface which causes enhanced in-plane magnetization.

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Dielectric-Spectroscopic and ac Conductivity Investigations on Manganese Doped Layered Na1.9Li0.1Ti3O7 Ceramics (망간이 혼입된 층상구조 Na1.9Li0.1Ti3O7 세라믹스의 유전율 ‒ 분광법과 교류 전도도 측정 연구)

  • Pal, Dharmendra;Pandey, J.L.;Shripal
    • Journal of the Korean Chemical Society
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    • v.53 no.1
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    • pp.42-50
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    • 2009
  • The dielectric-spectroscopic and ac conductivity studies firstly carried out on layered manganese doped Sodium Lithium Trititanates ($Na_{1.9}Li_{0.1}Ti_3O_7$). The dependence of loss tangent (Tan$\delta$), relative permittivity ($\varepsilon_r$) and ac conductivity ($\sigma_{ac}$) in temperature range 373-723K and frequency range 100Hz-1MHz studied on doped derivatives. Various conduction mechanisms are involved during temperature range of study like electronic hopping conduction in lowest temperature region, for MSLT-1 and MSLT-2. The hindered interlayer ionic conduction exists with electronic hopping conduction for MSLT-3. The associated interlayer ionic conduction exists in mid temperature region for all doped derivatives. In highest temperature region modified interlayer ionic conduction along with the polaronic conduction, exist for MSLT-1, MSLT-2, and only modified interlayer ionic conduction for MSLT-3. The loss tangent (Tan$\delta$) in manganese-doped derivatives of layered $Na_{1.9}Li_{0.1}Ti_3O_7$ ceramic may be due to contribution of electric conduction, dipole orientation, and space charge polarization. The corresponding increase in the values of relative permittivity may be due to increase in number of dipoles in the interlayer space while the corresponding decrease in the values of relative permittivity may be due to the increase in the leakage current due to the higher doping.

Genetic Diversity of High-Quality Rice Cultivars Based on SSR Markers Linked to Blast Resistance Genes (도열병 저항성 유전자와 연관된 SSR 마커를 이용한 양질미 품종의 유전적 다양성)

  • Huhn-Pal Moon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.3
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    • pp.251-255
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    • 2004
  • The epidemics of rice blast which occurred in south parts of Korea during the period from 1999 to 2001 and damaged several high quality rice cultivars developed using "Milyang 95" and/or "Milyang 96" as a parent. Genetic diversity of 23 rice cultivars including "Milyang 95" and it's relatives was assessed using 54 simple sequence repeats (SSR) markers reported to be linked to major blast resistance genes. Fifty-four SSR markers representing fifty-seven loci in the rice genome detected polymorphism among the 23 cultivars and revealed a total of 170 alleles with an average of 3.0 alleles per primer, The number of amplified bands ranged from 1 to 7. Several SSR markers including RM249, RM206 and OSR20 were informative for assessing the genetic diversity of relatively closed japonica rice cultivars. The 23 cultivars were classified into four groups by cluster analysis based on Nei's genetic distances, and the cultivars developed from same parents showed a tendency to cluster together that is consistant with genealogical information. High quality rice cultivars, Daesanbyeo, Donganbyeo, and Milyang 95 belonged to the same cluster, At the loci, RM254 and OSR32, all of the cultivars derived from the crosses using "Milyang 95" shared same alleles, suggesting that these japonica cultivars might carry alleles that are identical by descent. Evaluation of 23 rice cultivars against blast needs to be confirmed regarding the relationship between genotype and blast resistance.p between genotype and blast resistance.

A STUDY ON THE EFFECT OF POLYMERIZATION SHRINKAGE OF SEVERAL COMPOSITE RESIN USING STRAIN GAUGE (스트레인 게이지를 이용한 수종 수복재의 중합수축 영향 평가)

  • Lee, In-Cheon;Kim, Jong-Soo;Yoo, Seung-Hoon
    • Journal of the korean academy of Pediatric Dentistry
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    • v.36 no.1
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    • pp.20-29
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    • 2009
  • This study was performed to evaluate the effect of the shrinkage stress induced by polymerization process of several light curing filling materials according to filling methods. High power light curing unit which has a plasma arc lamp was used and filling materials used were Filtek $Z-250^{(R)}$ composite resin, $Dyract^{(R)}$ AP compomer and $Tetric^{(R)}$ Flow flowable composite resin. Cavities were prepared on the permanent molars with width 3 mm, height 3 mm and depth 1.5 mm and the filling materials were filled with 1 step, 2 step layering technique and 3 step oblique filling methods. The results can be summarized as follows; 1. Strain values showed rapid increase from the start of light curing followed by gradual decrease afterwards with time. 2. Although the shrinkage stress value of $Z-250^{(R)}$ were shown to be relatively higher than $Dyract^{(R)}$ AP and $Tetric^{(R)}$ Flow, no statistically significant could be found between tested materials(p>0.05). 3. There were no statistically significant difference between 3 filling methods when using $Dyract^{(R)}$ AP and $Z-250^{(R)}$(p>0.05). 4. There were no statistically significant difference between shrinkage stress values obtained from samples prepared by different filling methods and materials(p>0.05).

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Investigation on Optimal Aeration Rate for Minimizing Odor Emission during Composting of Poultry Manure with Sawdust (계분톱밥 퇴비화시 악취발생의 최소화를 위한 적정 공기주입을 구명)

  • Kang, Hang-Won;Park, Hyang-Mee;Ko, Jee-Yeon;Lee, Jae-Saeng;Kim, Min-Tae;Kang, Ui-Gum;Lee, Dong-Chang;Moon, Huhn-Pal
    • Korean Journal of Environmental Agriculture
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    • v.20 no.4
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    • pp.225-231
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    • 2001
  • This study was conducted to find out the optimal aeration rates for minimizing odor emission and for increasing biological activities during composting of livestock manure in the enclosed bench-scale reactor system. It was treated with the mixture of poultry manure and sawdust controlled the initial water content of 60%, then aerated continuously at four different aeration rates (0.1, 0.2, 0.4 and 0.6 L/min/kg dry-solids). The average emitted concentration of ammonia in 0.6 L/min/kg dry-solids during composting reached the level of 40% in comparison with that of 0.2 L/min/kg dry-solids. In cases of sulfur compounds such as hydrogen sulfide, methylmercaptan and ethylmercaptan, their concentrations decreased with increasing aeration rates and the emission time was shortened. But they didn't detect in the treatment of 0.6 L/min/kg dry-solids. The biological activity for composting showed a trend of increasing as aeration rates increased. The treatment of 0.6 L/min/kg dry-solids gave the highest biological activity and the best compost quality.

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Surface Study on the Supported Molten Salt Catalyst (담지된 금속염 혼합물 촉매의 표면 연구)

  • Kim, Jong Pal;Lee, Kwang Hyun
    • Applied Chemistry for Engineering
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    • v.20 no.4
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    • pp.381-385
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    • 2009
  • A basic objective is the preparation and surface studies of supported molten salt catalysts because molten salts can stay as the liquid phase in the range of the ordinary reaction temperature. Many kinds of metal salt mixtures for the formation of molten salt phase are appliable but CuCl and KCl were selected in this study because Cu is considered catalytically reactive in many reactons. The loading of the molten salt was selected as 25 vol% of the total pore volume of ${\gamma}-alumina$ to provide reasonable exposed surface area. The surface structure of catalysts containing molten salts in the ${\gamma}-alumina$ was studied using scanning electron microscopy (SEM) and energy-dispersive spectroscopy (EDS). CuCl and KCl were added into the ${\gamma}-alumina$ using concentrated hydrochloric acid solution by the impregnation technique. The surfaces of the prepared catalysts before and after heat treatments were compared and they suggested that the heat treatment of catalysts helped the formation of molten-salt although the surface compositions of CuCl and KCl were not uniform.

Introgression of Oryza minuta into Rice, Oryza sativa (벼 Oryza sativa x O. minuta 여교배 계통에서 이입 염색체단편 검정)

  • Jin Feng Xue;Kang Kyung-Ho;Kwon Soo-Jin;Jeong Oh-Young;Le Heung Linh;Moon Huhn-Pal;Ahn Sang-Nag
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.6
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    • pp.533-538
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    • 2004
  • An introgression line, WH79006 was produced from a single plant from $BC_5F_3$ families from a cross between Hwaseongbyeo used as a recurrent parent and O. minuta (BBCC, Ace. No. 101154) as a donor parent, which was subsequently self-pollinated for three generations. WH79006 resembled the O. sativa parent, Hwaseongbyeo. However it differed from Hwaseongbyeo in several traits including days to heading, culm length, grain size, spikelets per panicle and fertility. These differences in the traits between WH79006 and Hwaseongbyeo can be attributed to the O. minuta introgressions. To detect the introgressions, 294 SSR markers of known chromosomal position have been used. At least, 28 introgressed chromosomal segments have been identified using SSR markers and they map to all chromosomes except chromosome 2. The size of the introgressed segments ranged from 4 to 35cM. A QTL related to culm length was detected using 75 $F_2$ plants from the Hwaseongbyeo/WH79006 cross. This QTL, cl6 located on chromosome 6 explained $9.6\%$ of the total phenotypic variation in the population. This QTL has not been detected in the previous QTL studies between Oryza sativa cultivars, indicating potentially novel alleles from O. minutan.