Browse > Article

Genetic Diversity of High-Quality Rice Cultivars Based on SSR Markers Linked to Blast Resistance Genes  

Huhn-Pal Moon (작물시험장)
Publication Information
KOREAN JOURNAL OF CROP SCIENCE / v.49, no.3, 2004 , pp. 251-255 More about this Journal
Abstract
The epidemics of rice blast which occurred in south parts of Korea during the period from 1999 to 2001 and damaged several high quality rice cultivars developed using "Milyang 95" and/or "Milyang 96" as a parent. Genetic diversity of 23 rice cultivars including "Milyang 95" and it's relatives was assessed using 54 simple sequence repeats (SSR) markers reported to be linked to major blast resistance genes. Fifty-four SSR markers representing fifty-seven loci in the rice genome detected polymorphism among the 23 cultivars and revealed a total of 170 alleles with an average of 3.0 alleles per primer, The number of amplified bands ranged from 1 to 7. Several SSR markers including RM249, RM206 and OSR20 were informative for assessing the genetic diversity of relatively closed japonica rice cultivars. The 23 cultivars were classified into four groups by cluster analysis based on Nei's genetic distances, and the cultivars developed from same parents showed a tendency to cluster together that is consistant with genealogical information. High quality rice cultivars, Daesanbyeo, Donganbyeo, and Milyang 95 belonged to the same cluster, At the loci, RM254 and OSR32, all of the cultivars derived from the crosses using "Milyang 95" shared same alleles, suggesting that these japonica cultivars might carry alleles that are identical by descent. Evaluation of 23 rice cultivars against blast needs to be confirmed regarding the relationship between genotype and blast resistance.p between genotype and blast resistance.
Keywords
genetic diversity; high quality; rice; simple sequence repeats (SSRs); blast; genetic background;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Ji, H. S., H. J. Koh, S. U. Park, and S. R. McCouch. 1998. Varietal identification in japonica rice using microsatellite DNA markers. Korean J. Breeding 30(4) : 350-360
2 National Academy of Sciences. 1972. Genetic vulnerability of major crops. National Academy of Science, Washington, DC.
3 Wu, K. S. and S. D. Tanksley. 1993. Abundance, polymorphism and genetic mapping of microsatellites in rice. Mol. Gen. Genet. 241 : 225-235   DOI   PUBMED
4 Han, S. S., J. D. Ryu, H. S. Shim, S. W. Lee, Y. K. Hong, and K. H. Cha. 2001. Breakdown of resistance of rice cultivars by new race Kl1117a and race distribution of rice blast fungus during 1999-2000 in Korea. Korean Research in Plant Disease 7(2) : 86-92
5 Kwon, S. J., S. N. Ahn, H. C. Choi, and H. P. Moon. 1999. Evaluation of genetic relationship and fingerprinting of rice varieties using microsatellite and RAPD markers. Korean J. Crop Science 44(2) : 112-116
6 Kim K. H., S. Y. Cho, H. P. Moon, and H. C. Choi. 1994. Breeding strategy for improvement and diversification of grain quality in rice (in Korean). Korean J. Breeding 26 (Supp. 2) : 3-19
7 Kwon, S. J., S. N. Ahn, J. P. Suh, Y. C. Cho, H. C. Hong, Y. K. Kim, H. G. Hwang, H. P. Moon, and H. C. Choi. 2000a. Genetic mapping and identification of QTLs related to agronomic traits in a japonica cross of rice. Proc. 1st Plant Mol. Genet. Breeding, Poster 12. Seoul Nat'l Univ., Suwon, Korea(Sept. 1, 2000)
8 Kwon, S. J., S. N. Ahn, J. P Suh, H. C. Hong, Y. K. Kim, H. G. Hwang, H. P. Moon, and H. C. Choi. 2000b. Genetic diversity of Korean native rice varieties. Korean J. Breeding 32(2): 186-193
9 McCouch, S. R., S. Temnykh, A. Lukashova, J. Coburn, G. DeClerck, S. Cartinhour, S. Harrington, M. Thomson, E. Septiningsih, M. Semon, P. Moncada, and Li Jiming. 2001. Microsatellite markers in rice: abundance, diversity, and applications. In: Rice Genetics IV p 117-135
10 Kwon, S. J., S. N. Ahn, C. I .Yang, H. C. Hong, Y. K. Kim, J. P. Suh, H. G. Hwang, H. P. Moon, and H. C. Choi. 2000c. Genetic diversity of Korean rice cultivars. In:Abstract. International Rice Genetics Symposium, International Rice Research Institute, Los Banos, Philippines, Oct. 2000. p41
11 Temnykh, S., G. DeClerck, A. Lukashova, L. Lipovich, S. Cartinhour, and S. R. McCouch. 2001. Computational and experimental analysis of microsatellites in rice (Oryza sativa L.): frequency length variation, transposon association, and genetic marker potential. Genome Res. 11:1441-1452   DOI   ScienceOn
12 Nei, M. 1973. Analysis of gene diversity in subdivided population. Proc. Natl. Acad. Sci. U.S.A. 70(12): 3321-3323   DOI   ScienceOn
13 Panaud O., X. Chen, and S. R. McCouch. 1996. Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Olyza sativa L.). Mol. Gen. Genet. 252 : 597-607
14 Sebastian, L. S., L. R. Hipolito, and J. S. Garcia. 1998. Molecular marker analysis of the contribution made by landraces to modem Philippine rice cultivars. SABRAO J. Breed. and Genet. 30(2) : 73-82
15 Han, S. S., S. H. Choi, D. S. Ra, and M. Y. Eun. 1988. Analysis of rapid increase of rice blast fungus race Kl-409 in Korea. Korean J. Plant Phathology 14 : 705-709
16 Kwon, S. J., S. N. Ahn, H. C. Hong, Y. C. Cho, J. P. Suh, Y. K. Kim, K. H. Kang, S. S. Han, H. C. Choi, H. P. Moon, and H. G. Hwang. 2002. Identification of DNA markers linked to resistance genes to rice blast (Pyricularia grisea Sacc.) Korean J. Breeding 34(2): 105-110
17 Olufowote, J. O., Y. Xu, X. Chen, W. D. Park, H. M. Beachell, R. H. Dilday, M. Goto, and S. R. McCouch. 1997. Comparative evaluation of within-cultivar variation of rice (Oryza sativa L.) using microsatellite and RFLP markers. Genome 40: 370-378   DOI   ScienceOn
18 Ahn, S. N., Y. K. Kim, H. C. Hong, S. S. Han, S. J. Kwon, H. C. Choi, H. P. Moon, and S. R. McCouch. 2000. Molecular Mapping of a New Gene for Resistance to Rice Blast (Pyricularia gresea Sacc.). Euphytica 116: 17-22   DOI   ScienceOn
19 Causse, M. A., T.M. Fulton, Y. G. Cho, S. N. Ahn, J. Chunwongse, K. S. Wu, J. H. Xiao, Z. H. Yu, P. C. Ronald, S. E. Harrington, G. Second, S. R. McCouch, and S. D. Tanksley. 1994. Saturated molecular map of the rice genome based on an interspecific backcross population. Genetics 138 : 1251-1274