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Production of yuzu granules using enzyme treated yuzu pulp powder and evaluation of its physiochemical and functional characterization (유자박 식이섬유를 이용한 유자과립 제조 및 이화학적 특성조사)

  • Seong, Hyeon Jun;Lee, Bo-Bae;Kim, Duck-Hyun;Lee, Seung-Hyun;Ha, Ji-Young;Nam, Seung-Hee
    • Korean Journal of Food Science and Technology
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    • v.53 no.4
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    • pp.382-390
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    • 2021
  • In this study, solubilized yuzu pulp powder (EYP) was produced using enzyme treated yuzu pulp powder (YP) and used to manufacture yuzu granules (0-20% EYP content). The physicochemical, product stability, and functional properties of Yuzu granules were compared among five enzyme treatments. Among the five treatments, CL had the highest YP solubilization yield (48.68%). Microstructural observation of EYP using FE-SEM revealed that its surface became irregular and porous after enzymatic treatment. Compared to YP, EYP had 2 times lower insoluble dietary fibers and 3 times lower hemicellulose and cellulose content. Among the yuzu granules, IV (yuzu granules with 15% EYP) had an excellent water and oil holding capacity and flowability. IV granule had the highest narirutin and hesperidin content of 3.4 mg and 2.2 mg/g DW, respectively and the highest antioxidant (68.4%) and tyrosinase inhibitory activities (82.5%). Therefore, EYP or granule with EYP can be used as a functional component in food industry or pharmaceutical field.

Economic Analysis, Growth and Pests of Wheat (Triticum aestivum L.) in Gelatin·Chitin Microorganisms-treated Organic Culture (젤라틴·키틴분해미생물을 이용한 밀 유기재배와 관행재배의 생육, 병해충 발생조사 및 경제성 분석)

  • Ahn, Philip;Lee, Jiho;Cha, Kwang-Hong;Seo, Dong-Jun;An, Kyu-Nam;Yoon, Chang-Yong;Kim, Kil-Yong;Jung, Woo-Jin
    • Korean Journal of Organic Agriculture
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    • v.29 no.2
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    • pp.223-240
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    • 2021
  • This study was carried out to investigate the economic value of organic wheat production using gelatin·chitin microorganisms in Gwangsan-gu, Gwangju city. The soil condition of experiment field was clay loam Jisan series. The organically cultivated fields were sprayed gelatin and chitin degrading bacteria. The test was performed at conventionally cultivated field and organically cultivated field. Emergence of weed on organically cultivated field was significantly higher than conventionally cultivated field which sprayed herbicide before seeding. Weed emergence have a critical impact on grain yield. Occurrence of diseases and insect pests were higher than conventionally cultivated fields. In 2019, the amount of lodging in conventionally cultivated field were higher than conventionally cultivated field. In 2020, lodging and wet injury were occur in both field. Comparing yield element between organically and conventionally cultivated experimental area, grain yield in organically cultivated field was shown slightly higher amount than conventionally cultivated field. However in the actual yield of 2019, organically cultivated field shows 20% deceased yield because of overgrown weed. In 2020, weed emergence and yellow mosaic virus by wet injury cause 30% decease in the grain yield in organically cultivated field. Content of protein, carbohydrates, ash, water and fat in the grain were not different significance. In 2019, net incomes of conventionally cultivated wheat was 461,031 won/0.1 ha while organically cultivated wheat was 443,437 won/0.1 ha. In the rate of income, conventionally cultivated field was 83.0% as against organically cultivated field (73.3%). In 2020, net incomes of organically cultivated wheat was 437,812 won/0.1 ha while conventionally cultivated wheat was 418,281 won/0.1 ha. In the rate of income, conventionally cultivated field was 81.6% as against organically cultivated field (73.0%).

A Study on the Botany of New Natural Habitats of Abeliophyllum distichum Nakai in the Byeonsanbando National Park (변산반도국립공원 내 새로운 미선나무 자생지의 식물학적 연구)

  • Oh, Hyun Kyung;Soh, Min Seok;Rho, Jae Hyun
    • Korean Journal of Heritage: History & Science
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    • v.44 no.2
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    • pp.4-25
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    • 2011
  • This study was performed in 2010 to examine the flora and vegetation structure and chemical characteristics of soil in the growing community of Abeliophyllum distichum, located in the Byeonsanbando National Park. This Abeliophyllum distichum community has more individual numbers in Cheongrim-ni and Jungkye-ri, Byeonsan-myeon, and Buan-gun area, which is designated as a Natural Monument (No. 370), and also where the habitat conditions for Abeliophyllum distichum is more favorable. The authors recorded 100 taxa with 45 families, 82 genus, 93 species, 4 varieties, and 3 forms. Among them, species such as Abeliophyllum distichum (critically endangered), Asarum maculatum (near threatened) and Chionanthus retusa (near threatened), which are categorized as rare plants, were recorded. According to the list of Korean endemic plants, 4 taxa, particularly Philadelphus schrenckii, Abeliophyllum distichum, Weigela subsessilis, and Lonicera subsessili, were recorded. The community of Abeliophyllum distichum is located in the northwest slope of Baekcheon watershed and the community is comprised of healthy soil. The community structure was classified into three: the Castanea crenata community, Zelkova serrata community, and Quercus serrata community. The Castanea crenata community is composed of the Cornus walteri, Platycarya strobilacea, Zelkova serrata, Rhamnella frangulioides, arranged in terms of importance percentage. The Zelkova serrata community is composed of Celtis sinensis, Quercus aliena, Styrax japonica, and Acer pseudo-sieboldianum, also according to importance percentage. As for the Quercus serrata community, it is composed of Quercus variabilis, Castanea crenata, and Prunus sargentii, also arranged in terms of importance percentage. The importance percentage of Abeliophyllum distichum is 6.6% in the Castanea crenata community, 5.6% in the Zelkova serrata community and 5.1% in the Quercus serrata community. Moreover, in order of chemical characteristics of soil pH, electrical conductivity, available phosphoric, organic matter, and exchangeable cation (K, Ca, Mg) are analyzed. The No. 3 site was relatively higher than other districts of the same chemical characteristics of soil.

Microbiological Safety Evaluation on Ice Cream and Ice Pop Products (빙과류의 품목별 제품의 미생물학적 안전성 평가)

  • Yu, Jeong-Wan;Kim, So-Hyun;Hong, Dong-Lee;Kim, Hyeon-Jae;Jeong, Eun-Joo;Lee, Jae-Hwa;Yang, Ji-Young;Lee, Yang-Bong
    • Journal of Food Hygiene and Safety
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    • v.34 no.4
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    • pp.367-373
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    • 2019
  • In order to evaluate the microbiological safety of ice cream products, the total viable bacterial counts were measured in 6 kinds of ice pops, 5 kinds of non-milk fat ice cream, and 5 kinds of milk fat ice cream, sold in local markets. In addition, E. coli, S. aureus, B. cereus, and L. monocytogenes were artificially inoculated in three types of ice cream products and stored at $-5^{\circ}C$, $-10^{\circ}C$, and $-18^{\circ}C$, respectively, and after inoculation, viable cells were measured periodically. As a result of the total viable count, about 1~2 log CFU/mL was detected in 16 kinds of ice cream products. As a result of inoculation with microorganisms at various temperatures, the number of viable cells decreased as the storage period became longer, and the higher the storage temperature, the faster the microorganisms died. Especially, the microorganisms were killed faster in the ice pop products than in the other ice cream products, and the microorganisms were killed relatively slower in the milk ice cream. L. monocytogenes and S. aureus were relatively stable in frozen conditions compared to other microorganisms. The microbial contamination of commercial ice cream was lower than the allowable standard of the Korean Food Code. Microorganisms did not proliferate when the microorganism was inoculated at freezing temperature. Therefore, it is expected that the microbiological safety of frozen foods will be ensured if the sanitary control and disinfection of raw materials are thoroughly carried out during the production of frozen confections and the temperature control during distribution and storage is well maintained.

Growth Conditions and Maintenance of Natural Monument Old Big Trees in Gangwondo (강원도 내 천연기념물 노거수의 생육환경 및 관리현황)

  • Heo, Bok Soo;Kim, Hyo Jeong;Lee, Won Ho;Kang, Hee Jin
    • Korean Journal of Heritage: History & Science
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    • v.44 no.1
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    • pp.182-195
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    • 2011
  • Old big tree transcends the simple meaning of trees as they are the natural monuments that embody the people's history and culture of this land. The Cultural Heritage Administration of Korea (CHA) defines and protects old big tree based not only on the size of the tree but also on its definitive cultural and natural factors such as value, implications, and originality. This research aims to identify and analyze the habitation and measurements, the soil conditions, as well as the protective facilities of 14 old big tree spread throughout the Gangwondo. The research examined the soundness of the arboreal form, the degree of damage on the bark, as well as the quantity of leafs levels to evaluate the overall condition of growth and development. Then, the tree height, the eastwest and south-north width of the crown, the sternum circumference, and the root circumference were recorded to identify the measurements of the selected old big tree. Also, the research analyzed 9 elements such as soil texture, pH levels, and organic contents in order to determine the scientific characteristics of soil upon which the trees draw nutrition. Finally, the research evaluated supporting pillars, lightning rods, iron pulling-facilities, as well as notice boards to study the protective facilities of old big tree. The research procedures revealed that 78.57% of the old big tree identified as natural monuments in the Gangwondo have average to above average habitation conditions. Also, the average tree height reaches 18.80m above ground while the average root circumference measures 7.34m. Moreover, the results of the scientific analyses conducted on the soil elements determined that most chemical values were within the defined levels, with the exception of phosphoric acid levels falling within the hyper-nutritious range. Finally, although most of the protective facilities did support the habitation of the Old big tree, there were some supporting pillars and iron pulling-facilities that actually damaged the trees. This research means to provide foundational reference data for the analysis of the habitation and management conditions of natural monument old big tree within the Gangwondo. A follow-up topic led by the results of this research is the identification and analysis of correlating environmental elements that affect the habitation of the old big tree.

Studies on sterile filters in the preparation of N-13 ammonia injection (N-13 암모니아 주사액 제조 시 멸균필터의 흡착율 차이에 관한 비교 평가)

  • Oh, Chang Bum;Kim, Si Hwal;Cha, Min Jung;Shin, Jin;Ji, Yong Gi;Choi, Sung Ook
    • The Korean Journal of Nuclear Medicine Technology
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    • v.23 no.1
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    • pp.64-68
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    • 2019
  • Purpose In the preparation process for N-13 Ammonia injections, there were radioactive medicines adsorbed on filters remarkably. Hereby, we have compared the adsorption rate and quality test on Millex GS filter and Satorious Minisart filter, both representatively hydrophilic sterilizing filters, also evaluated which filter is more accommodative for N-13 Ammonia injection. Materials and Methods The filters used for sterilization of N-13 Ammonia injections were Millex GS filter($0.22{\mu}m$) mand Satorious Minisart filter ($0.2{\mu}m$), which are generally used to strain aqueous solutions. After the N-13 Ammonia passes through each sterilization filter, the adsorption rate of the filter (n=10) is determined by measuring not only the radioactivity through the filter also the amount of radioactivity remaining in it using a Dose Calibrator. The N-13 Ammonia injections after each filter is tested by the quality control test to conform to the Samsung Medical Center standard. Results The ratio of radioactivity passed through Millex GS indicated $29.0{\pm}17.6%$. Satorious Minisart filters output was $80.9{\pm}3.2%$, respectively. Each ratio of radioactivity adsorbed on the sterile filter was $71.0{\pm}17.6%$ for Millex GS and $19.1{\pm}3.2%$ for the Satorious Minisart filters, respectively. Furthermore, on the ratio of filtered radioactivity, Using Satorious Minisart filter showed about 2.8 times higher than using Millex GS filter. The quality testing of N-13 Ammonia injections through each filter met the Samsung Medical Center standard. Conclusion The Millex GS filter is composed of cellulose acetate and cellulose nitrate, whereas the Satorious Minisart filter if composed only of cellulose acetate. Therefore, the presence of cellulose nitrate in the membrane seems to have made differences. Therefore, the use of Satorious Minisart filter in the preparation of N-13 Ammonia injection solution minimized the loss of radioactive medicines due to filter adsorption, thereby improving the synthesis yield.

Conditioned Media of RAW 264.7 Cells Stimulated with Phellinus linteus Extract Regulates the Epithelial-mesenchymal Transition in Prostate Cancer Cells (상황버섯에 의해 활성화된 RAW 264.7 대식세포주 배양액의 인간 전립선암 세포주의 epithelial-mesenchymal transition 조절)

  • Kang, Taewoo;An, Hyun-Hee;Park, Sul-Gi;Yu, Sun-Nyoung;Hwang, You-Lim;Kim, Ji-Won;Ahn, Soon-Cheol
    • Journal of Life Science
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    • v.29 no.8
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    • pp.904-915
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    • 2019
  • Prostate cancer (PCa) is one of the most metastatic tumor. Although hormone therapy or surgical castration is mostly conducted to treat PCa, it has a lot of side effects. Recently, many researchers have been exploring the tumor microenvironment to remedy these circumstances. Immune cells, especially macrophages, are an important composition of the tumor microenvironment. Under normal conditions, macrophages exhibit mild tumoricidal activity against tumors. However, once activated by interferon gamma or lipopolysaccharides, macrophages can kill cancer cells directly or indirectly by secreting cytokines and chemokines. In this study, murine macrophage RAW 264.7 cells were treated with Phellinus linteus extract. To analyze their pro-inflammatory phenotype, we were used several assays such as a real-time polymerase chain reaction, an enzyme-linked immunosorbent and nitric oxide assay. Prostate cancer cells were treated with the RAW 264.7-conditioned media, which was identified as a pro-inflammatory nature, for 48 h, and the expression of epithelial-mesenchymal transition (EMT)-related genes was determined. Not only N-cadherin, Snail, Twist, Slug, and Cadherin 11, which are mechenchymal-related proteins, were decrease, but epithelial marker of E-cadherin was increased. In addition, the mRNA level of vimentin, ccl2, and vegfa were decreased, as the EMT is closely related to the migration and invasion of cancer cells. In conclusion, the RAW 264.7-conditioned media stimulated with P. linteus extract inhibited migration and invasion and regulated the EMT pathway in human prostate cancer cells.

The Vegetation Effect of under Neutralizing Layer Type on the Acid Drainage Slope (산성배수 비탈면의 중화층 종류에 따른 녹화효과)

  • Cho, Sung Rok;Kim, Jae Hwan
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.39 no.4
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    • pp.493-503
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    • 2019
  • This study is composed of nine treatments [Control : "no neutralizing layer+vegetation layer" 3 cm, Treatment 1 : "no neutralizing layer+vegetation layer" 5 cm, Treatment 2 : "no neutralizing layer+vegetation layer" 7 cm, Treatment 3 :"neutralizing layer (cement 3 %)+ vegetation layer (cement 1 %)" 3 cm, Treatment 4 : "neutralizing layer (cement 3 %)+vegetation layer (cement 1 %)" 5 cm, Treatment 5 : "neutralizing layer (cement 3 %)+vegetation layer (cement 1 %)" 7 cm, Treatment 6 : "neutralizing layer [$(Ca{\cdot}Mg)CO_3$] +vegetation layer" 3 cm, Treatment 7 : "neutralizing layer [$(Ca{\cdot}Mg)CO_3$]+vegetation layer" 5 cm, Treatment 8 : "neutralizing layer [$(Ca{\cdot}Mg)CO_3$]+vegetation layer" 7 cm] to find out the vegetation effects according to neutralizing layer types of the acid drainage slope. There were no significant differences observed in soil hardness and soil moisture content of neutralizing layer type while highly difference of moisture content was observed according to the neutralizing and vegetation layer thickness. As for soil acidity, strong acid was shown in the control, treatment 1 and treatment 2. Neutralizing effects were outstanding in treatments of 3, 4, 5 (cement treatment group), 6, 7 and 8 (limestone treatment group). Concerning plants growth characteristics, surface coverage rates, number of germinating woody plants, plant height, and plant root status, there were excellent effects observed in the experimental groups mixed with cement (treatments 3, 4 and 5) and limestone (treatments 6, 7 and 8). At the initial stage, however, plant roots were negatively affected in cement layer treatments of 3, 4 and 5. However, no difference was shown in each layer thickness on the acid drainage slope whereas 3~5 cm thickness neutralizing layer was appropriate in consideration of economic feasibility.

A Study of Improvement of Skin Condition and Sensory Efficacy by Periodic Application of L-α-Amino Acid (L-α-아미노산의 주기적 도포에 의한 피부개선 효능과 소비자 체감 효능 연구)

  • Kwon, Koo Chul;Lee, Sung Woo;Ahn, Byungjun;Kang, Nae-Gyu;Park, Sun Gyoo;Park, Sang Wook
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.45 no.1
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    • pp.9-17
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    • 2019
  • In this study, $L-{\alpha}-amino$ acid, which is a constituent of natural moisturizing factor, was applied to skin in periodic cycle to improve skin tone and texture roughness. Based on the polarity of the alkyl group (R) of the $L-{\alpha}-amino$ acid, the acid was categorized into two groups and their efficacy was studied. As a result, it was found that the improvement rate of $L-{\alpha}-amino$ acid complex with polar alkyl group ($L-{\alpha}-AAC-1$) is 21% higher than that of $L-{\alpha}-amino$ acid complex with non-polar alkyl group ($L-{\alpha}-AAC-2$). For clinical trials, emulsions containing $L-{\alpha}-amino$ acid complex ($L-{\alpha}-AAC-1$) were applied to the randomly selected 20 to 40 year old female participants, as an experimental group, on the right facial cheek once per day for 8 weeks, and emulsions without $L-{\alpha}-amino$ acid complex, as a control group, were applied to the left facial cheek in the same way. Improvements in skin tone were measured using $JANUS^{(R)}$ equipment and analyzed using image analysis software. Skin texture improvement was measured and analyzed mechanically using the phaseshift rapid in-vivo measurement of the skin (PRIMOS) equipment. As a result, improvements of skin tone and skin texture were 11.7% and 6.7%, respectively. In addition, a questionnaire survey was conducted to the participants on the aesthetic improvement and the degree of the feeling of skin improvement. The results suggest that $L-{\alpha}-amino$ acid can be used as a cosmetic substance that can provide aesthetic satisfaction through physiological skin tone and texture roughness.

A Study on the Possibility of Recycling Coir Organic Substrates for using Strawberry Hydroponics Media (토마토 폐배지를 딸기 수경재배 배지로 재이용 가능성 연구)

  • Lee, Gyu-Bin;Park, Young-Hoon;Choi, Young-Whan;Son, Beung-Gu;Kim, Jooh-Yup;Kang, Nam-Jun;Kang, Jum-Soon
    • Journal of Korea Society of Waste Management
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    • v.34 no.2
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    • pp.205-213
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    • 2017
  • The current study was performed to investigate the effect of recycling coir substrates on the growth, fruit yield, and quality of strawberry plants. Analysis of physical properties revealed that the pH of a fresh coir substrate was 5.04 while those of substrates reused for one and two years were 5.20 and 5.33, respectively. The electrical conductivity (EC) of a new substrate was as high as $4.58dS{\cdot}m^{-1}$. This can cause salt stress after transplanting. The EC tended to decrease as the substrate was recycled, and the EC of a two-year recycled substrate was $1.48dS{\cdot}m^{-1}$. The fresh substrate had lower nitrogen and calcium concentrations, but higher phosphate, potassium, and sodium concentrations than the recycled coir substrate. The coir substrates recycled for one or two years maintained better chemical properties for plant growth than the fresh substrate. Strawberry growth varied depending on the number of years that the coir substrate was recycled. In general, strawberries grown in substrates that had been reused for two years did better than those grown in substrates that had been reused once or were fresh. Ninety days after transplanting, a plant grown in a substrate that had been reused for two years contained 25 leaves, which was 3.6 more than with a fresh substrate. In addition, the plants grown in a substrate that had been reused for two years exhibited larger leaf areas than those grown in other substrates. Coir substrates that had been reused for one year increased the number and area of leaves, but not as much as the substrate that had been reused for two years. One- and two-year reused coir substrates increased the weight of strawberries produced relative to the unused substrate, but the difference was not statistically significant. The plants grown in two-year reused substrates were longer and wider, as well. Also, the number of fruits per plant was higher when substrates were reused. Specifically, the number of fruits per plant was 28.7 with a two-year reused substrate, but only 22.2 with a fresh substrate. The fruit color indices (as represented by their Hunter L, a, b values) were not considerably affected by recycling of the coir substrate. The Hunter L value, which indicates the brightness of the fruit, did not change significantly when the substrate was recycled. Neither Hunter a (red) nor b (yellow) values were changed by recycling. In addition, there were no significant changes in the hardnesses, acidities, or soluble solid-acid ratios of fruits grown in recycled substrates. Thus, it is thought that recycling the coir substrate does not affect measures of fruit quality such as color, hardness, and sugar content. Overall, reuse of coir substrates from hydroponic culture as high-bed strawberry growth substrates would solve the problems of new substrate costs and the disposal of substrates that had been used once.