• Journal of Microbiology and Biotechnology
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• 제25권2호
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• pp.238-246
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• 2015

A unicellular red microalga was isolated from environmental freshwater in Korea, and its morphological, molecular, and biochemical properties were characterized. Morphological analysis revealed that the isolate was a unicellular biflagellated green microalga that formed a non-motile, thick-walled palmelloid or red aplanospore. To determine the taxonomical position of the isolate, its 18S rRNA and rbcL genes were sequenced and phylogenetic analysis was performed. We found that the isolate was clustered together with other related Haematococcus strains showing differences in the rbcL gene. Therefore, the isolated microalga was classified into the genus Haematococcus, and finally designated Haematococcus sp. KORDI03. The microalga could be cultivated in various culture media under a broad range of pH and temperature conditions. Compositions of the microalgal cellular components were analyzed, and its protein, carbohydrate, and lipid compositions were estimated to be 21.1 ± 0.2%, 48.8 ± 1.8%, and 22.2 ± 0.9%, respectively. In addition, D-glucose and D-mannose were the dominant monosaccharides in the isolate, and its amino acids were composed mainly of aspartic acid, glutamic acid, alanine, and leucine. Moreover, several polyunsaturated fatty acids accounted for about 80% of the total fatty acids in Haematococcus sp. KORDI03, and the astaxanthin content in the red aplanospores was estimated to be 1.8% of the dry cell weight. To the best of our knowledge, this is the first report of an Haematococcus sp. isolated from Korea, which may be used for bioresource production in the microalgal industry.

• Journal of Ginseng Research
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• 제33권1호
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• pp.13-25
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• 2009

연구 결과는 아래와 같다. 풍화토양에서 혈암 및 화강암지역이 높은 원소가, 화강암지역이 낮은 원소가 많았다. 이는 토양내의 이들 원소를 함유할 수 있는 광물의 존재와, 함량으로 설명이 될 수 있다. 밭 토양에서 천매암 지역이 높은 원소가, 화강암 지역은 낮은 원소가 많았다. 즉 이 관계는 토양 내 함유되어 있는 광물내 원소의 특성과, 풍화작용, 인삼의 생육기간 동안 발생하는 토양내의 물리, 화학적 작용 탓으로 설명할 수 있다. 모암에서 혈암이 높은 원소가, 화강암이 낮은 원소가 많았다. 각 암석별 원소 함량 차이는 암석 중 존재하는 광물의 특성으로 설명이 가능하다. 인삼은 각 지역별 함량 비교에서 높은 값이 혈암 지역의 T1, 천매암 지역의 Cs, B, 화강암 지역의 Be, Cd에서 나타났다. 이 들 관계는 토양 중 이들 원소들을 함유하고 있는 광물의 종류 및 함량 차이에 의한 영향과 토양 중에서 원소의 거동에 영향을 주는 물리, 화학적 특성 차이 탓이다. 동일 지역 연생 차이별 성분 비교에서 높은 원소가 혈암 지역은 2 년생, 천매암 및 화강암 지역은 4 년생에서, 타 지역 동일 연생별 평균 비교에서 높은 원소가 2 년생은 혈암지역이, 3, 4년생은 화강암 지역에서 나타났다. 이는 토양 중 광물의 특성, pH, 온도 등의 물리, 화학적 변수들이 특정연령 및 토양 지역에 더 잘 반영되었음을 암시한다. 풍화토와 밭토양의 관계에서 혈암 지역이 대부분 원소에서 풍화토가 높고, 천매암 및 화강암 지역은 밭토양이 높았다. 이는 풍화토는 개방된 상황에서 풍화를 받는 지역인데 반해, 밭토양은 인위적인 영향을 받았고, 해가림 밑에서 인삼이 다년생으로 자라면서 원소들이 인삼 내에서 흡수되고, 해가림 밑에서 풍화에 의한 영향을 받았을 것을 암시한다. 토양과 인삼의 성분과의 관계에 토양이 인삼에 비해 Cs, T1, Be에서 높고, 커다란 차이가 나타났다. 3 토양 중 화강암 지역의 인삼이 다른 두 지역보다 원소 함량에서 토양의 조성에 가까움을 암시한다.

• Journal of Plant Biology
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• 제11권2호
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• pp.1-30
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• 1968

The Han River was investigated monthly from April through September, 1967 at Chun-chon reservoir, Shin-yean, So-yang, Chung-pyong reservoir, Yang-soo-ri and Gi-doo-won to clarify the phytoplankton and to study its seasonal variation. At the same time, vertical distribution of phytoplankton was also studied from June through September at Chun-chon and Chung-pyong reservoir. The results obtained are as follows: As the environmental conditions, water temperature, dissolved oxygen, pH, and transparency were determined. The plankton samples collected from six stations were identified and classified by Engler's classification system. It resulted in 4 phyla, 4 classes, 3 subclasses, 13 orders, 27 families, 5 tribes, 71 genera, 222 species, 1 subspecies, 51 varieties and 2 forms. The total number of phytoplankton identified was 276. Of them, 84 species, 1 subspecies 26 varieties, 2 forms were recorded in this paper for the first time. Considering with the habitat of the total number of phytoplanktons, the highest percentage of Limnoplankton existed in the Chung-pyong reservoir. It suggests that hte Chung-pyong reservoir is becoming to have the character of lake. The planktons appeared commonly during six months at six station belong to Chrysophyta including 22 species in 11 genera. Among these 22 species, Navicula, consists of 6 species, was the most abundant. The number of species identified uniquely at each station were 5 species in the Chun-chon reservoir, 6 in Chung-pyong and Yang-soo-ri, 8 at Shin-yean, 11 at So-yang, and 18 at Gi-doo-won. The number of species occurred at all stations were 29 in spring, 42 in Autumn and during investigation period. The maximum production of total phytoplankton was generally shown in the late spring and the early fall with differences in quantity at six collecting statins. In the Chun-chon reservoir, the high concentration of approximately 1, 190, 000 cells per litter in the months of August and September was due to the increase of Chrysophyte genus, Melosira and the highest concentration of approximately 2, 000, 000 in July at Gi-doo-won was caused Chlorophyte genus, Secenedesmus. The Chun-chon reservoir of three years old was different from the Chung-pyong reservoir of 25 years old with respect to increase in Melosira genus in August and September. After the completion of the Ui-am reservoir, though there was an increase in total density, the number of species of phytoplankton was not changed. It suggests that Ui-am reservoir is similar to the Chun-chon reservoir under the influence of the latter. The density of total phytoplankton was generally high at the depth of 2m to 5m for the two reservoirs.

• Korean Chemical Engineering Research
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• 제44권5호
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• pp.547-554
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• 2006

응집공정에서 교반조건과 응집제 주입농도에 따른 알루미늄 가수분해종 변화에 대한 실험결과 다음과 같은 결론을 얻을 수가 있었다. 알루미늄 표준용액을 이용하여 모노머성 알루미늄과 페론 반응을 살펴본 결과 반응초기에 급격한 반응률을 보이며 반응시간 3분 정도에 평형에 도달함을 알 수 있었다. 순수의 경우 교반시간에 따른 영향은 거의 나타나지 않고 있으며 거의 일정한 반응률을 보이고 있었다. 상수원수의 경우 입자상 물질과 유기물의 존재함에 따라 응집제 주입시 수중에서 형성되는 알루미늄 가수분해종이 입자상 물질 및 유기물과 우선적으로 반응하기 때문에 형성되는 알루미늄 가수분해 종에 대한 반응률이 교반시간에 따라 다르게 나타났다. 응집제 주입량이 증가할수록 페론과 반응율이 빠르게 일어나나 일정한 시간이 경과한 후에 반응율을 살펴보면 응집제 주입량이 증가할수록 반응이 느리게 나타났다. 순수의 경우 교반시간에 따른 Ka 값은 교반시간이 증가할수록 Ka 값은 감소함을 알 수 있으며 응집제 주입량의 영향은 크게 나타나지 않고 있다. 그러나 Kb의 경우 응집제 주입량이 증가할수록 반응속도 상수값이 낮아지는 경향을 보이고 있으며, 마찬가지로 교반시간이 증가할수록 Kb 값은 감소함을 알 수 있다. 상수원수를 사용한 경우 순수와 마찬가지로 교반시간에 따른 Ka, Kb값 은 교반시간이 증가할수록 감소하였다. 그러나 응집제 주입량이 증가할수록 Ka 값은 감소하였다.

• 한국식품영양과학회지
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• 제2권1호
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• pp.1-21
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• 1973

The muscle of abalone, Notohaliotis discus (REEVE), and top-shell, Turbo cornutus Solander, were examined for protein composition. Then paramyosins which are known as one of the important structural protein of the muscle fibrils were isolated from the both muscle and their physico-chemical properties such as solubility, salting-out behaviour, intrinsic viscosity, ATPase activity, etc. involving amino acid composition and N-terminal amino acid residues were investigated to elucidate phylogenie characteristics more intensively from the viewpoint of comparative biochemistry. The analysis of protein composition resulted in the following estimations: abalone muscle; water-soluble protein of 22 %, salt-soluble protein, 34%, alkali-soluble protein, 20%, and stroma protein, 24%, and top-shell muscle; water-soluble protein of 16%, salt-soluble protein, 30%, alkali-soluble protein, 29%, and stroma protein, 25%, respectively. It is demonstrated in sedimentation analysis that paramyosin and myosin-actomyosin account for approximately 65% and 35% of the salt-soluble protein of abalone, and that the composition of both sediments in top-shell was approximately 70% and 30%, respectively. The ultracentrifugally homogenous paramyosins isolated essentially according to Bailey's ethanol-dried method from both of the muscle showed a $S^{\circ}_{20,w}$ of 3. 14s for abalone and a $S^{\circ}_{20,w}$ of 3.50s for top-shell. The both paramyosins were commonly rich in arginine, aspartic acid, and glutamic acid, while scarcely contained proline and tryptophan, in rough accord with the other paramyosins thus far reported. It is clear that these gastropod paramyosins showed of having the characteristic N-terminal amino acid residues such as N-aspartic acid, N-valine, N-serine, and N-threonine in common. The abalone paramyosin completely salted in with KCl beyond $0.35{\mu}$ and the top-shell paramyosin beyond $0.30{\mu}$. The abalone paramyosin was salted-out between 18% and 30% saturation of ammonium sulphate and the top-shell paramyosin between 22% and 29% saturation. The intrinsic viscosities at abalone and top-shell paramyosins at $25^{\circ}C$ were estimated respectively to be 3.1 dl/g and 2.6 dl/g showing somewhat higher than the values for some other paramyosins from lamellibranchs. In regard with the ATPase activity, the para myosin specimens did not exhibit any significant activity over through the pH conditions of 5 to 9.5. irrespective of the presence of $Ca^{++}$ or $Mg^{++}$. So was the case with the abalone paramyosin prepared by a slightly modified Bailey's wet-extraction method.

• 한국환경과학회지
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• 제29권2호
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• pp.123-132
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• 2020

Seasonal changes in the CO₂ fixation rate and water-use efficiency in the leaves of six evergreen and two deciduous broad-leaved tree species on Jeju Island, Korea, were measured using a portable photosynthesis analyzer, to identify which species are most efficient in taking up CO₂ from the air. The CO₂ fixation rate was high in the deciduous species in spring and summer and decreased in fall, whereas it was high in the evergreen species in summer and fall and decreased in winter. The rate remained high in the deciduous tree Prunus yedoensis from spring to fall (> 7.1 μmol CO₂/m₂/s) and in two evergreen trees, Castanopsis cuspidata var. sieboldii and Cinnamomum camphora, in summer and fall (7.0 9.9 μmol CO₂/㎡/s). Therefore, these tree species fix atmospheric CO₂ effectively. The water-use efficiency was higher in evergreen species than in deciduous species regardless of the season. Exceptionally, it was high in the deciduous species Zelkova serrata in spring and summer (> 100 μmol CO₂/mol H₂O), suggesting that Z. serrata is a useful tree for dry conditions due to its tolerance of water stress. The regressions of the CO₂ fixation rate versus the evaporation rate and stomatal conductance were linear and non-linear, respectively. This suggests that the stomatal activity of leaves plays an important part in CO₂ fixation of plants. In conclusion, C. cuspidata var. sieboldii, C. camphora, and P. yedoensis should be planted along roads or in urban spaces for the greening of cities and mitigation of CO₂ concentrations in the air.

• 한국수산과학회지
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• 제46권5호
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• pp.607-618
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• 2013

Marine macroalgal community structures and characteristics of ocean environmental factors were examined seasonally at four sites in Jeju Island, Korea, from March to November 2012. A total of 71 macroalgal species were identified, including 9 green, 7 brown, and 55 red algae. Peyssonnelia capensis occurred at all study sites and in all seasons. The average annual biomass of seaweed was 991.84 g wet $wt/m^2$, with seasonal variations from 543.80 g in autumn to 1,284.17 $g/m^2$ in summer. A green alga, Codium coactum, was the dominant species, occupying 21.31% (211.39 $g/m^2$) of the total algal biomass in Jeju Island. Subdominant species were Ecklonia cava and Lithophyllum okamurae, comprising 20.85% (206.75 $g/m^2$) and 19.64% (194.75 $g/m^2$), respectively, of the total algal biomass in Jeju Island. The vertical distribution of subtidal seaweeds was represented by L. okamurae at 5 m depth, C. coactum at 10 m depth, E. cava at 5-10 m depth and P. capensis at the 20 m depth level. In the present study, crustose coralline algae, which predominated on barren ground, were subdominant species at all study sites. Community indices varied between 0.51-0.63 for dominance index (DI), 5.53-8.14 for richness index (R), 0.51-0.63 for evenness index (J'), and 2.04-2.32 for diversity index (H'). On the basis of seaweed biomass and community indices, Sinchang was the best preserved coastal area, showing maximal values in biomass, and evenness- and diversity-indices, and minimal value in the dominance index, representing stable environmental conditions. In contrast, the Onpyung and Topyeong sites, located near tourist venues such as Udo and Seogwipo were relatively poor habitats based on community indices and biomass. The present results could imply that climate changes alter seaweed community structure, and long-term monitoring of the study sites is required.

• 대한한의학회지
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• 제22권2호
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• pp.53-63
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• 2001

목적 : 귀전우 Euonymus alatus (Thunb.) Sieb로부터 얻은 추출물에 대하여 생리활성(항산화 활성)을 검토하였고, $amyloid-{\beta}{\;}(A{\beta})$가 지니는 Rat의 배양 Hepatocyte 세포독성에 대한 세포보호효과 및 세포막 지질의 과산화에 미치는 영향을 검토하였다. 방법 : 귀전우 추출물과 항산화제인 BHT. ascorbic acid 및 ${\alpha}-tocopherol$을 각각 linoleic acid에 소정량 첨가하고 $50^{\circ}C$에서 일정기간 저장하여 linoleic acid의 산화 안정성을 비교 검토하였으며, 또한 유지 자동산화의 촉진인자인 금속이온($Fe^{3+},{\;}Zn^{2+}$) 을 첨가하여 귀전우 추출물의 금속이온봉쇄효과를 조사하였다. 결과 :세포성지질의 과산화의 지표인 malondialdehyde(MDA)생성이 A{\beta}처리로 크게 증가하였으나 세포막파괴에 의한 세포파괴의 전형적인 현상이 귀전우 열수 추출물의 전처리와 후처리로 크게 감소되었다. 귀전우 추출물의 농도에 따른 항산화 효과는 전 농도별에서 높은 항산화 효과를 나타내었다. 그리고 시간 경과에 따른 항산화 효과에 있어서도 정도의 차이는 있지만 각 농도(5mg, 10mg 및 25mg)에서 유도기의 연장효과가 상당히 우수한 것으로 나타났다. 귀전우 추출물과 항산화제와의 비교에 있어서는 합성 항산화제인 BHT 및 ascorbic acid 만큼 우수한 항산화력을 나타내었으며, 천연 항산화제인 ${\alpha}-tocopherol$ 보다는 아주 우수한 것으로 나타났다. 귀전우 추출물의 금속이온 봉쇄효과에 있어서는 linoleic acid에 귀전우 추출물과 $Fe^{3+}$ 및 $Zn^{2+}$을 첨가한 군이 귀전우 추출물을 첨가하지 않은 군보다 항산화 효과가 아주 우수한 것으로 나타나 금속이온 봉쇄력이 강한 것으로 나타났다. 결론 : 귀전우 열수 추출물은 $A{\beta}$ 의한 세포파괴를 감소시키는 활성을 가지며 예방 및 보호효과를 나타내었고, hepatocyte에 대한 보호효과와 세포막지질 과산화의 저해 및 $A{\beta}$처리와 같은 독성에 대한 적응능력 향상을 통한 세포보호효과를 주는 것으로 간세포 보호 등의 임상적 응용에 그 효과가 기대된다.

• The Plant Pathology Journal
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• 제35권6호
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• pp.662-673
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• 2019

Root-knot nematodes (RKNs) are among the most destructive plant-parasites worldwide, and RKN control has been attempted mainly using chemical nematicides. However, these chemical nematicides have negative effects on humans and the environment, thus necessitating the search for eco-friendly alternative RKN control methods. Here, we screened nematicidal lactic acid bacteria (LAB) isolated from kimchi and evaluated their efficacy as biocontrol agents against RKNs. Of 237 bacterial strains, Lactobacillus brevis WiKim0069 showed the strongest nematicidal activity against the second-stage juveniles (J2) of Meloidogyne incognita, M. arenaria, and M. hapla and inhibited the egg hatch of M. incognita. The culture filtrate of WiKim0069 had a pH of 4.2 and contained acetic acid (11,190 ㎍/ml), lactic acid (7,790 ㎍/ml), malic acid (470 ㎍/ml), and succinic acid (660 ㎍/ml). An artificial mixture of the four organic acids produced by WiKim0069 also induced 98% M. incognita J2 mortality at a concentration of 1.25%, indicating that its nematicidal activity was derived mainly from the four organic acids. Application of WiKim0069 culture filtrate suppressed the formation of galls and egg masses on tomato roots by M. incognita in a dose-dependent manner in a pot experiment. The fermentation broth of WiKim0069 also reduced gall formation on melon under field conditions, with a higher efficacy (62.8%) than that of fosthiazate (32.8%). This study is the first report to identify the effectiveness of kimchi LAB against RKNs and to demonstrate that the organic acids produced by LAB can be used for the RKN management.

• 생태와환경
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• 제46권2호
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• pp.251-264
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• 2013

2010년 6월부터 2011년 10월까지 고등균류를 조사한 결과, 총 2계 3문 6강 15목 34과 59속 107종이 동정되었으며, 담자균문은 28과 51속 99종, 자낭균문은 5과 7속 7종 및 아메바문은 1과 1속 1종인 것으로 조사되었다. 외생균근성 버섯을 조사한 결과 총 10과 17속 49종 총 801개체가 조사되었으며 이 중 애기꾀꼬리버섯이 198개체로 가장 많이 발생되었고 꾀꼬리버섯 66개체, 색시졸각버섯 53개체 순이었다. 종 분포가 높은 균류는 광대버섯과가 14종으로 가장 많이 나타났으며, 그물버섯과 12종, 무당버섯과 11종 순으로 조사되었다. 조사시기별 분포에서는 7월과 8월에 가장 다양한 외생균근성 버섯이 발생되었는데 이는 높은 온도 및 강수량이 많은 시기와 밀접한 관계가 있는 것으로 나타났다. 조사구별 분포에서는 조사구 I인 소나무 군락 (8과 13속 30종)과 II인 참나무 군락 (8과 12속 30종)에서 가장 높았고, 조사구 IV (1과 1속 1종)와 V의 곰솔 식재지 에서는 발생하지 않았다. 외생균근성 버섯 발생에 영향을 주는 기후환경 요인으로는 대기온도 (평균온도, 최저온도, 최고온도)에서, 토양환경 요인으로는 토양 산도와 유기물함량에서 밀접한 관계가 있는 것으로 나타났다.