• Title/Summary/Keyword: pH adjustment

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Landfill Leachate Treatment and Boron Removal by Reverse Osmosis (RO막을 이용한 매립지 침출수 처리 및 붕소 제거)

  • Jung, Soojung;Na, Sukhyun;Bae, Sangok;Lim, Byungjin
    • Journal of Korean Society on Water Environment
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    • v.28 no.5
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    • pp.657-662
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    • 2012
  • This study was carried out to evaluate the removal rate of organic and inorganic matters from landfill leachate using pre-treatment process as coagulation and limonite adsorption, and membrane process as RO (reverse osmosis) and NF(nanofiltration). By adding limonite adsorption as pre-treatment process, about 40% of organic matters in leachate was removed through pre-treatment process and 74.7% of boron was removed after RO process without pH adjustment. The rejection rate of boron in RO process mainly depends on the pH and increased at pH value of 10. RO process was performed as two stage system adjusting pH condtion to 7 and 10 in second RO stage for boron removal. Most (>90%) of TOC, Cl- and inorganic matters as Ca was rejected in first RO stage, the residue was rejected in second RO and the rejection rate was above 97%. Considering economic efficiency of operation cost, NF substituted for the first RO and total removal rate of TOC was above 90%. Through RO system toxicity to Daphnia in leachate was removed completely.

Effect of Cryoprotectants on Quality Properties of Chicken Breast Surimi Manufactured by pH Adjustment (냉동변성방지제 조건에 따른 닭가슴살 수리미의 저장특성)

  • Jin, S.K.;Kim, I.S.;Choi, Y.J.;Park, G.B.;Yang, H.S.;Kim, B.G.
    • Journal of Animal Science and Technology
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    • v.50 no.1
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    • pp.99-110
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    • 2008
  • This study was conducted to determine the effect of pH adjustment and the addition of cryoprotectants on the storage properties of chicken breast surimi. Surimi as a control was prepared with Alaska pollack by two times of washing treatment and addition of cryoprotectants(4% sugar, 5% sorbitol and 0.3% polyphosphate). Three types of surimi were manufactured by different cryoprotectant conditions(T1: 5% sorbitol+0.3% polyphosphate, T2: 4% sugar+5% sorbitol+0.3% polyphosphate, T3: 2% salt+4% sugar+5% sorbitol+0.3% polyphosphate) and frozen after adjusting the surimi to pH 11.0. The amino acid and saturated fatty acid composition were significantly higher in the control. EAA(essential amino acid), FAA(amino acid in relation to flavor), SAAA(amino acid in relation to saccarinity) and FRAA (fragrant amino acid) were significantly higher in the control. The TBARS(thiobarbituric acid reactive substances) and VBN(volatile basic nitrogen) were increased with storage times. TBARS and VBN values were significantly higher in the control and T3 than the other treatments. The VBN was significantly higher in control and T3 than other surimi samples. Chicken breast surimi adjusted to pH 11.0 had higher in microorganisms than the control, and T1 sample had the highest total plate count. Sensory evaluations were significantly higher in the control and T3 than the other samples. Especially, the overall acceptability of T3 was similar to the control.

pH Effect at Thermophilic Solubilization Pretreatment of Food Waste in Two Phase Anaerobic Digestion (2상 혐기성 소화에서 음식물쓰레기의 고온 가용화 전처리 pH 영향)

  • Lee, Won-Soo;Kang, Young-Jun;Seo, Gyu-Tae
    • Journal of Korean Society of Environmental Engineers
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    • v.38 no.8
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    • pp.452-458
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    • 2016
  • The study on pH control at the themophilic solubilization (pretreatment process) was investigated in order to improve the methane gas production of two phase anaerobic digestion of food waste. From a batch experiment, it was observed that the solubilization efficiency was increased from 26.2% to 47.1% and 55.6% by the pH increament from $4.20{\pm}0.40$ (without pH control) to $7.00{\pm}0.50$, and $12.00{\pm}0.50$, respectively. However there was immaterial increase (8.5%) in solubilization efficiency when the pH was increased from $7.00{\pm}0.50$ to $12.00{\pm}0.50$. The two phase anaerobic digestion system was operated for laboratory scale experiment under the solubilization condition of pH $4.20{\pm}0.40$ (Run1) and $7.00{\pm}0.50$ (Run2). Higher soluble chemical oxygen demand (SCOD) and total volatile fatty acid (TVFA) concentration were observed in Run2 throughout the system resulted by the solubilization effect at the pH $7.00{\pm}0.50$. The TVFA concentration in acidogenic reactor was 18.4 g/L which was 1.8 times higher than the result of Run1. Consequently the methane gas production was enhanced to 0.333 L/g VS in the methanogenic reactor, which is 18% higher than the result (0.282 L/g VS) of Run1.

Effects of pH-treated Fish Sarcoplasmic Proteins on the Functional Properties of Chicken Myofibrillar Protein Gel Mediated by Microbial Transglutaminase

  • Hemung, Bung-Orn;Chin, Koo Bok
    • Food Science of Animal Resources
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    • v.34 no.3
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    • pp.307-315
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    • 2014
  • pH adjustment would be of advantage in improving the water holding capacity of muscle proteins. The objective of this study was to evaluate the addition of fish sarcoplasmic protein (SP) solution, which was adjusted to pH 3.0 or 12.0, neutralized to pH 7.0, and lyophilized to obtain the acid- and alkaline-treated SP samples, on the functional properties of the chicken myofibrillar protein induced by microbial transglutaminase (MTG). The solubility of alkaline-treated SP was higher than that of the acid counterpart; however, those values of the two pH-treated samples were lower than that of normal SP (p<0.05). All SP solutions were mixed with myofibrillar proteins (MP) extracted from chicken breast, and incubated with MTG. The shear stresses of MP with acid- and alkaline-treated SP were higher than that of normal SP. The thermal stability of MP mixture reduced upon adding SP, regardless of the pH treatment. The breaking force of MP gels with acid-treated SP increased more than those of alkaline-treated SP, while normal SP showed the highest value. The MP gel lightness increased, but cooking loss reduced, with the addition of SP. Smooth microstructure of the gel surface was observed. These results indicated that adjusting the pH of SP improved the water holding capacity of chicken myofibrillar proteins induced by MTG.

Study on the Improvement of Land Clearing Methods by Bulldozer & Fertilization of Cleared Soil (불도우저에 의한 개간 공법의 개선과 숙지화에 관한 연구)

  • Hwang, Eun
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.17 no.1
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    • pp.3627-3641
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    • 1975
  • The Government is trying to increase total food grain yield to meet national self sufficiency by means of increasing unit yield as well as extending crop land, and this year he set the target of 321,000 hectare of forest to clear for cropping. This study was carried to investigate the most efficient method of clearing hillock by bulldozer, and successful method to develope yielding potential of newly cleared land in short term. Since the conventional land clearing method is just earth leveling and root removing neglecting top soil treatment, the growth of crop was poor and farmer tends not to care the land. The top-soil-furrowing method is applied through out this study, that is advantageous especially for the land having shallow top soil and low fertility like Korean forest. In this study, various operating method were tried to find out most efficient method separately in connection with the land slope less than 25 percent and over, and several fertilizing methods to develop yielding potential. The results are as follows; 1) For the natural land slope utilization method, applicable to the land having less than 25 percent slope, reverse operating was more efficient than using forward gear of bulldozer. The operating time was 3 hour 32 minutes and 36 seconds using forward gear was 2 hour 32 minutes and 30 seconds for reverse gear operation per 1,000 square meter. 2) Bulldozer having angle blade adjustment needed 7hr 15min. for constructing of terrace per 10a compaire with the one having angle & tilt adjustment needed 6hr 4min for same operations. Specially there is significant difference for operation time of first period (earth cutting) such as bulldozer having angle blade adjustment needed 3hr 56min compaired with the one having angle & tilt adjustment 3hr 59min. In construction of terrace, the bull-dozer having tilting and angle blade adjustment was most suitable and performed efficiently. 3) For the fertilizer application treatment, the grass (Ladino clover) yield in first year was almost same as ordinary field's in the plot applied(N.P.K+lime+manure) while none fertilizer plot showed one tenth of it, and (N.P.K.+lime) applied plot yielded on third. 4) The effect of different land clearing method to yield showed significant difference between each treatment especially in the first year, and the conventional method was the lowest. In the second year, still conventional terracing plot yielded only half of ordinary field while the other plots showed as same as ordinary field's. 5) The downward top soil treatment plot showed most rapid improvement in soil structure during one year physio chemically, it showed increase in pH rate and organic composition, and the soil changed gradually from loam to sand-loam and the moisture content increased against the pF rate, and it gives good condition to grow hay due to the increase of field water capacity with higher available water content. 6) Since the soil of tested area was granite, the rate of soil errosion was increased about 2 to 5 percent influencing in soil structure more sand reducing clay content, and an optimum contour farming method should be prepared as a counter measure of errosion.

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Development and evaluation of probiotic delivery systems using the rennet-induced gelation of milk proteins

  • Ha, Ho-Kyung;Hong, Ji-Young;Ayu, Istifiani Lola;Lee, Mee-Ryung;Lee, Won-Jae
    • Journal of Animal Science and Technology
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    • v.63 no.5
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    • pp.1182-1193
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    • 2021
  • The aims of this study were to develop a milk protein-based probiotic delivery system using a modified rennet-induced gelation method and to determine how the skim milk powder concentration level and pH, which can affect the rennet-induced intra- and inter-molecular association of milk proteins, affect the physicochemical properties of the probiotic delivery systems, such as the particle size, size distribution, encapsulation efficiency, and viability of probiotics in simulated gastrointestinal tract. To prepare a milk protein-based delivery system, skim milk powder was used as a source of milk proteins with various concentration levels from 3 to 10% (w/w) and rennet was added to skim milk solutions followed by adjustment of pH from 5.4 or 6.2. Lactobacillus rhamnosus GG was used as a probiotic culture. In confocal laser scanning microscopic images, globular particles with a size ranging from 10 ㎛ to 20 ㎛ were observed, indicating that milk protein-based probiotic delivery systems were successfully created. When the skim milk powder concentration was increased from 3 to 10% (w/w), the size of the delivery system was significantly (p < 0.05) increased from 27.5 to 44.4 ㎛, while a significant (p < 0.05) increase in size from 26.3 to 34.5 ㎛ was observed as the pH was increased from 5.4 to 6.4. An increase in skim milk powder concentration level and a decrease in pH led to a significant (p < 0.05) increase in the encapsulation efficiency of probiotics. The viability of probiotics in a simulated stomach condition was increased when probiotics were encapsulated in milk protein-based delivery systems. An increase in the skim milk powder concentration and a decrease in pH resulted in an increase in the viability of probiotics in simulated stomach conditions. It was concluded that the protein content by modulating skim milk powder concentration level and pH were the key manufacturing variables affecting the physicochemical properties of milk protein-based probiotic delivery systems.

Alkaliphilic Endoxylanase from Lignocellulolytic Microbial Consortium Metagenome for Biobleaching of Eucalyptus Pulp

  • Weerachavangkul, Chawannapak;Laothanachareon, Thanaporn;Boonyapakron, Katewadee;Wongwilaiwalin, Sarunyou;Nimchua, Thidarat;Eurwilaichitr, Lily;Pootanakit, Kusol;Igarashi, Yasuo;Champreda, Verawat
    • Journal of Microbiology and Biotechnology
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    • v.22 no.12
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    • pp.1636-1643
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    • 2012
  • Enzymatic pre-bleaching by modification of pulp fibers with xylanases is an attractive approach to reduce the consumption of toxic bleaching chemicals in the paper industry. In this study, an alkaliphilic endoxylanase gene was isolated from metagenomic DNA of a structurally stable thermophilic lignocellulose-degrading microbial consortium using amplification with conserved glycosyl hydrolase family 10 primers and subsequent genome walking. The full-length xylanase showed 78% sequence identity to an endo-${\beta}$-1,4-xylanase of Clostridium phytofermentans and was expressed in a mature form with an N-terminal His6 tag fusion in Escherichia coli. The recombinant xylanase Xyn3F was thermotolerant and alkaliphilic, working optimally at $65-70^{\circ}C$ with an optimal pH at 9-10 and retaining >80% activity at pH 9, $60^{\circ}C$ for 1 h. Xyn3F showed a $V_{max}$ of 2,327 IU/mg and $K_m$ of 3.5 mg/ml on birchwood xylan. Pre-bleaching of industrial eucalyptus pulp with no prior pH adjustment (pH 9) using Xyn3F at 50 IU/g dried pulp led to 4.5-5.1% increase in final pulp brightness and 90.4-102.4% increase in whiteness after a single-step hypochlorite bleaching over the untreated pulp, which allowed at least 20% decrease in hypochlorite consumption to achieve the same final bleaching indices. The alkaliphilic xylanase is promising for application in an environmentally friendly bleaching step of kraft and soda pulps with no requirement for pH adjustment, leading to improved economic feasibility of the process.

Improvement of Quality in Treated Water by the pH Adjustment of Raw Water (원수 pH 조정에 의한 정수 수질 개선)

  • Jeong, Gwan-Jo;Lee, Kyeong-Woo;Kim, Hyun-Hee;Jeong, Eui-Sun;Park, Hyeon;Han, Sun-Hee
    • Journal of Korean Society of Environmental Engineers
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    • v.32 no.5
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    • pp.469-476
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    • 2010
  • The purpose of the study is to find ways to decrease turbidity and residual aluminum by improving the efficiency of coagulation process through controlling the pH of the source water with $CO_2$ when the pH increases by algal bloom or by the characteristics of the source water. Water quality parameters were monitored before and after $CO_2$ addition in February, March, April, and December, when the pH of the source water is over 8.0 and constant regardless of day and night. Water quality parameters closely related with evaluation of treated drinking water quality were monitored in detail, e.g. aluminum, turbidity, particle counts, TOC, THMs, 2-MIB, and geosmin. According to the results, inorganic water quality parameters such as turbidity, particle counts, and aluminum were decreased due to improved efficiency of the coagulation process. It was concluded that the pH of the water in the arrival basin must be controlled below 7.4 by adding $CO_2$ when the pH of the source water increasing. By controlling pH with $CO_2$, the water quality could be maintained within the drinking water quality goal of Seoul City (<30 particle/mL of particle count, <0.05 NTU of turbidity and <0.02 mg/L of aluminum). The change of the pH could not affect the concentrations of DBP's (e.g., THMs, CH, and HAAs) and taste/odor causing compounds (e.g., 2-MIB and geosmin). 2-MIB and geosmin were affected more by their initial concentrations in the source water.

Enhanced Antibacterial Activity of Sodium Hypochlorite under Acidic pH Condition (산성 pH 조건에서 차아염소산나트륨의 항균 활성 향상)

  • Son, Hyeon-Bin;Bae, Won-Bin;Jhee, Kwang-Hwan
    • Microbiology and Biotechnology Letters
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    • v.50 no.2
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    • pp.211-217
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    • 2022
  • Sodium hypochlorite (NaClO) is a disinfectant widely used in hospitals and food industries because of its antimicrobial activity against not only bacteria but also fungi and virus. The antibacterial activity of NaClO lies in the maintenance of a stable hypochlorous acid (HClO) concentration, which is regulated by pH of the solution. HClO can easily penetrate bacterial cell membrane due to its chemical neutrality and the antibacterial activity of NaClO is thought to depend on the concentration of HClO in solution rather than hypochlorite ions (ClO-). In this study, we investigated the antibacterial activity of NaClO according to pH adjustment by means of time kill test and assays of Reactive Oxygen Species (ROS) and adenosine triphosphate (ATP) concentration changes before and after NaClO treatment. We also investigated that the degree of cell wall destruction through field emission scanning electron microscopy (FE-SEM). Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) exposed to 5 ppm NaClO at pH 5 exhibited 99.9% mortality. ROS production at pH 5 was 48% higher than that produced at pH 7. In addition, the ATP concentration in E. coli and S. aureus exposed to pH 5 decreased by 94% and 91%, respectively. As a result of FE-SEM, it was confirmed that the cell wall was destroyed in the bacteria by exposing to pH 5 NaClO. Taken together, our results indicate that the antibacterial activity of 5 ppm NaClO can be improved simply by adjusting the pH.

Establishment of Optimum Deposition Time in Electrophoretic Deposition

  • Kim, Sun-Il;Cho, Sung-Hwan;Lee, Jung-Ki;Kim, Hyung-Sun
    • Proceedings of the Materials Research Society of Korea Conference
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    • 2012.05a
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    • pp.107.2-107.2
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    • 2012
  • We used the electrophoretic deposition (EPD) process to fabricate a glass composite and investigated the EPD parameters to find the optimum deposition time by understanding the relationship among the process parameters of zeta potential (ZP), pH, deposition yield and saturation point. A binder and a dispersing agent were mixed properly with glass frit ($0.2{\sim}25{\mu}m$, d50=$8.77{\mu}m$) in an ethyl alcohol medium for the preparation of the slurry. The pH and ZP were in an inverse relationship to each other due to the generation of $H_3O^+$ ions with the addition of the dispersing agent in the slurry. The acidic nature of the dispersing agent was resulted in a decrease of the pH and an increase of the ZP. Otherwise, the pH increased with the addition of the glass frit in the slurry because $H_3O^+$ ions were absorbed on the glass frit. Therefore, the $OH^-$ ions correspondingly increased. The saturation point of EPD was strongly correlated with the variation of the pH in the slurry; this is caused by a chemical reaction between the ethyl alcohol and the ions that make up the glass frit. An adjustment of the pH variation and the saturation point in the slurry can be established with respect to the optimum deposition time in the slurry.

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